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PENGARUH 2-iP, BA, 2,4-D, DAN TDZ PADA EMBRIOGENESIS SOMATIK IN VITRO KOPI ROBUSTA UNGGUL LAMPUNG Dwi Hapsoro; Dwi Setiawan; Rahmadyah Hamiranti; Yusnita Yusnita
Jurnal Agrotek Tropika Vol 7, No 3 (2019): JAT September 2019
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Pengaruh 2-iP, BA, 2,4-D, dan TDZ pada Embriogenesis Somatik In Vitro Kopi Robusta Unggul Lampung. Embriogenesis somatik in vitro kopi Robusta terdiri dari empat tahap: induksi kalus primer, induksi kalusembriogenik, regenerasi embrio somatik, dan regenerasi planlet. Penelitian bertujuan mempelajari pengaruh zat pengatur tumbuh (ZPT) terhadap pembentukan kalus primer kopi Robusta unggul Lampung klon Komari. Eksplan potongan daun ditanam pada media induksi kalus primer dengan penambahan (mg/l): BA 1; 2-iP 1; 2-iP 1 + 2,4-D 0,5 ;2-iP 1 + 2,4-D 1 ;TDZ 1 + 2,4-D 0,5; dan TDZ 1 + 2,4-D 1 sebagai perlakuan. Percobaan menggunakan rancangan acak lengkap dengan tiga ulangan, 3 botol kultur per ulangan, 5 eksplan per botol. Hasil percobaan menunjukkan, pada 4 minggu setelah tanam, semua perlakuan dapat menginduksi kalus primer 100%, namun waktu terbentuknya kalus pertama kali dan bobot segar kalus primer berbeda-beda. Kalus primer paling cepat terbentuk pada perlakuan 2-iP (14,7 hari setelah tanam), sedangkan paling lambat (16,0-16,3) pada perlakuan TDZ + 0,5-1,0 mg/L 2,4-D dan BA 1 mg/l. Bobot segar kalus primer tertinggi didapat pada perlakuan 2-iP + 2,4-D (96-110 mg/eksplan) dan terkecil pada perlakuan 2-iP atau BA saja (24-18 mg/eksplan). Kalus primer berkembang menjadi kalus embriogenik waktu dipindah ke media induksi kalus embriogenik. Subkultur kalus embriogenik ke media regenerasi menghasilkan embrio somatik.

Pengaruh Spektrum Cahaya dan Lama Perendaman Benih Terhadap Pertumbuhan dan Hasil Microgreen Bunga Matahari (Helianthus annuus L.) Feni Maulida Fajrin; Rizka Novi Sesanti; Erie Maulana; Sismanto Sismanto; Rahmadyah Hamiranti
Journal of Horticulture Production Technology Vol 1 No 1 (2023)
Publisher : Politeknik Negeri Lampung

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Sunflowers are plants from the Asteraceae family which are popular as ornamental plants, as well as consumed in the form of microgreens. The problem with microgreen cultivation is that it requires light as a substitute for sunlight and is difficult to imbibition because it has a hard skin. The aims of this study were (1) to obtain the best light spectrum color on the growth and yield of sunflower microgreens, (2) to obtain the best soaking time for sunflower microgreen growth and yields, (3) to obtain the best interaction of light spectrum treatments and soaking time seed. The research method was arranged using a split plot experimental design in a randomized block design with 3 repetitions. The main plot are the light spectrum and the sub plots are the soaking time of the seeds. Variables observed: germination time, germination percentage, germination rate, microgreen height, sample weight per plot. The results showed that the white light spectrum produced the best germination percentage and germination rate, while the blue light spectrum produced the best microgreen height. Seed soaking time of 12 hours produced the best percentage of germination, germination rate, and weight per plot, while soaking time of 24 hours produced the best germination time and microgreen height. Blue light spectrum and 24 hours soaking time produced the best interaction on microgreen height, and weight per plot. White light spectrum and 12-hour soaking produced the best interaction on germination percentage.

Pengaruh Jenis Media Tanam dan Interval Pemberian Pupuk Daun Terhadap Pertumbuhan Plantlet Anggrek Dendrobium Blue Twist x Dendrobium Biggibum pada Tahap Aklimatisasi dan Pembesaran Pretty Aulia Dewi Fortuna; Sismanto Sismanto; Desi Maulida; Lisa Erfa; Ratih Rahhutami; Rahmadyah Hamiranti
Journal of Horticulture Production Technology Vol 1 No 2 (2023)
Publisher : Politeknik Negeri Lampung

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Dendrobium orchids have the potential to be developed because they have a variety of types, shapes and sizes. The choice of planting media and fertilization is an important factor in the success of orchid propagation, especially at the acclimatization stage. The aim of this research were determine the type of planting media and the best interval for giving foliar fertilizer and determine the best interactions and combinations of treatment of the type of planting media and the interval for giving foliar fertilizer on the growth of dendrobium orchid plantlets in the acclimatization and enlargement stages. This research used a factorial Randomized Block Design (RAK) with the first factor being the type of planting media and the second factor being the interval of foliar fertilizer application. The first factor includes M1: wood charcoal, M2: cocopeat, M3: wood charcoal + cocopeat 1:1, M4: wood charcoal + cocopeat 1:2, and M5: wood charcoal + cocopeat 1:3. The second factor includes P1: once every 4 days, P2: once every 8 days, P3: once every 12 days. The results of the research showed that the cocopeat planting media type provided the best percentage of acclimatization success for the Dendrobium Blue twist x Dendrobium Biggibum orchids. Providing foliar fertilizer at 8-day intervals provides the best increase in plantlet height and leaf width at the acclimatization and plantlet enlargement stages of Dendrobium Blue twist x Dendrobium bigibbum orchids. There was no interaction between the use of planting media type and the interval of foliar fertilizer application.

PEMBEKALAN KEWIRAUSAHAAN PNS PRA PURNABAKTI MELALUI PENYULUHAN BUDIDAYA HIDROPONIK SKALA RUMAH TANGGA DI LINGKUNGAN PEMERINTAH METRO Ali, Fahri; Tiara, Dede; Safitri, Betari; Hamiranti, Rahmadyah
Jurnal Pengabdian Nasional Vol 4 No 1 (2023)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jpn.v4i1.3702

Kegiatan pengabdian ini dilaksanakan di Local Education Center (LEC) Kartikatama, Metro, yang difasilitasi oleh Pemerintah Kota Metro untuk para pra purnabakti PNS di Lingkungan Pemerintah Kota Metro. Permasalahan utama yang dihadapi oleh cara pensiunan ini adalah hilangnya peran individu yang telah lama menjadi bagian dari dirinya, hal ini akan memicu stress, cemas, dan bahkan depresi jika individu tidak siap untuk menghadapi masa pensiun. Kegiatan pengabdian ini bertujuan untuk memberikan pengetahuan serta keterampilan tentang hidroponik skala rumah tangga pada PNS yang akan memasuki masa pensiun, sehingga dapat memunculkan jiwa wirausaha serta dapat berkarya di lingkungan masyarakat. Metode yang digunakan pada kegiatan ini adalah ceramah dan praktik langsung untuk menyemai benih sayuran, meracik nutrisi, dan merakit instalasi hidroponik. Selain itu dilakukan juga evaluasi untuk melihat ketercapaian kegiatan dan rencana tindak lanjut setelah kegiatan selesei. Target utama dari kegiatan ini adalah budidaya sayuran hidroponik skala rumah tangga pada kelompok PNS pra purnabakti di Lingkungan Pemerintah Kota Metro. Hasil dari kegiatan ini yaitu meningkatnya pengetahuan dan keterampilan peserta mengenai budidaya hidroponik skala rumah tangga, yang tergambar dari terpasangnya instalasi hidroponik sederhana oleh para peserta kegiatan. Instalasi yang telah terpasang diberikan kepada peserta agar peserta dapat terus berlatih dan menerapkan ilmu yang telah didapatkan selama kegiatan, sehingga tercipta kemandirian pangan (kebutuhan sayur skala rumah tangga terpenuhi) dan pada akhirnya terbentuk jiwa kewirausahaan di bidang sayuran berbasis hidroponik.

HYDROPONIC EXTENSION OF DRIP IRRIGATION METHOD FOR FRUIT AT PT GREAT GIANT PINEAPPLE Sismanto, Sismanto; Ali, Fahri; Safitri, Betari; Tiara, Dede; Prajaka, Nanang Wahyu; Hamiranti, Rahmadyah
Jurnal Pengabdian Nasional Vol 4 No 1 (2023)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jpn.v4i1.3708

PT Great Giant Pineapple (GGP) is the 3rd largest pineapple producer in the world. The cultivationactivities carried out by PT Great Giant Pineapple (PT GGP) still use a conventional system. PT. GGPdoes not yet have facilities and infrastructure that support the development of plant cultivation with ahydroponic system so that cooperation with Politeknik Negeri Lampung (Polinela) is needed in itsdevelopment. The problems faced by PT GGP, especially in the Research & Development section,can be resolved by conducting counseling on hydroponic drip irrigation methods for fruit, especiallymelon plants. By holding hydroponic counseling activities on drip irrigation methods for fruit at PTGreat Giant Pineapple, it is hoped that it can increase the knowledge and understanding of workersand staff at PT GGP on the cultivation and installation of hydroponic drip irrigation methods on melonplants. So that the development of hydroponic melon cultivation techniques with drip irrigationmethods can be carried out independently by partners.

Morfogenesis Anggrek Dendrobium ‘Gradita 31’ dengan Penggunaan Benzyladenine atau Thidiazuron Hamiranti, Rahmadyah; Prajaka, Nanang Wahyu; Yeni, Yeni; Erfa, Lisa; Maulida, Desi
Journal of Horticulture Production Technology Vol 1 No 2 (2023)
Publisher : Politeknik Negeri Lampung

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Dendrobium Gradita 31 has superior characteristics that are in demand by the market in Indonesia, so seeds with sameness quality, the same characteristics as the parent and in large quantities are needed. Tissue culture is one solution to support the availability of seeds in large quantities and have a high level of sameness quality. The process of regulating the type of ZPT and concentration in the culture media does not always produce the same regeneration pathway. This research aims to determine the regeneration pathway and to obtain the best type and concentration of cytokinin to support its regeneration pathway. This study used a completely randomized design with 3 replications. The first factor is the type of cytokinin, BA and Tdz. The second factor is the concentration of Benzyladenine and Thidiazuron, 1, 2 and 3 mg/l.. The data was analyzed using analysis of variance, and mean value was tested using tukey test 5%. The results of this study indicate that the regeneration pathway in the formation of adventitious shoots was indirect embryogenesis. The use of Tdz 3 mg/l was able to produce the highest percentage of somatic embryo formation and the highest number of somatic embryos. The fastest shoot formation time, shoot formation percentage and the highest number of shoots per clump were found BA 2 mg/l.

Morfogenesis Anggrek Dendrobium ‘Gradita 31’ dengan Penggunaan Benzyladenine atau Thidiazuron Hamiranti, Rahmadyah; Prajaka, Nanang Wahyu; Yeni, Yeni; Erfa, Lisa; Maulida, Desi
Journal of Horticulture Production Technology Vol 1 No 2 (2023)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Dendrobium Gradita 31 has superior characteristics that are in demand by the market in Indonesia, so seeds with sameness quality, the same characteristics as the parent and in large quantities are needed. Tissue culture is one solution to support the availability of seeds in large quantities and have a high level of sameness quality. The process of regulating the type of ZPT and concentration in the culture media does not always produce the same regeneration pathway. This research aims to determine the regeneration pathway and to obtain the best type and concentration of cytokinin to support its regeneration pathway. This study used a completely randomized design with 3 replications. The first factor is the type of cytokinin, BA and Tdz. The second factor is the concentration of Benzyladenine and Thidiazuron, 1, 2 and 3 mg/l.. The data was analyzed using analysis of variance, and mean value was tested using tukey test 5%. The results of this study indicate that the regeneration pathway in the formation of adventitious shoots was indirect embryogenesis. The use of Tdz 3 mg/l was able to produce the highest percentage of somatic embryo formation and the highest number of somatic embryos. The fastest shoot formation time, shoot formation percentage and the highest number of shoots per clump were found BA 2 mg/l.

Produksi Nutrisi Hidroponik ABC Mix Hayati Sesanti, Rizka Novi; Ali, Fahri; Darma, Wika Anrya; Tiara, Dede; Rahhutami, Ratih; Yeni, Yeni; Prajaka, Nanang Wahyu; Febria, Dila; Safitri, Betari; Hamiranti, Rahmadyah; Putri, Sekar Utami; Maulida, Desi
Journal of Horticulture Production Technology Vol 2 No 1 (2024)
Publisher : Politeknik Negeri Lampung

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ABC mix biological hydroponic nutrition is an innovation by developing the AB Mix hydroponic nutrition formulation (based on 100% chemicals) in collaboration with Liquid Organic Biofertilizer/LOB from LOB Plant PT. Great Giant Pineapple (PT.GGP). This invention is created in order to solve the problem of industries that want to produce hydroponic nutrients based on liquid organic biological fertilizer to reduce the concentration of chemicals in hydroponic nutrients. The aim of this invention are (1) to develop hydroponic nutrient products consisting of chemical fertilizers and biological fertilizers so that the use of chemicals can be reduced, (2) to produce patents granted regarding hydroponic nutrition based on chemical fertilizers and biological fertilizers, and (3) to apply the results of the invention to society and industry that requires the invention. The ABC Mix biological hydroponic nutrition product already has a patent with number IDS000006120 and has been implemented at Miftahul Huda Islamic Boarding School 606 through community service activities.

Perbanyakan Anggrek Dendrobium â€˜Gradita 31â€™melalui Embriogenesis Somatik Hamiranti, Rahmadyah
Savana Cendana Vol 9 No 2 (2024): Savana Cendana (SC) - April 2024
Publisher : Fakultas Pertanian, Sains, dan Kesehatan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/sc.v9i2.2383

Dendrobium tissue culture through somatic embryogenesis process is a solution for mass propagation. The concentration of PGR and the composition of basic media are important factors in somatic embryogenesis. This research aimed to obtain the best type of basic media and BA concentration for regeneration process through somatic embryogenesis on Dendrobium 'Gradita 31' orchid. This research used a completely randomized design with 3 replications arranged factorial. The first factor was the type of basic media, Â½ MS and MS. The second factor was concentration of cytokinin BA 1, 2 and 3 mg/L combined with NAA 0.5 mg/L. Each experimental unit consisted of 2 culture bottles, each contained 2 explants. The data from each variable was analyzed using analysis of variance, and continued with the BNJ test at a significance level of 0,05. The results of the research showed that the type of basic media had no significant effect on all variables. Concentration 2 mg/L of BA gave the highest response to the variables of percentage of somatic embryo formation, number of SE per explant and number of shoots per clump.

Acclimatization of Micro Shoot-tip Cutting Red Palestine Fig on Several Planting Media Compositions Erfa, Lisa; Hamiranti, Rahmadyah; Maulida, Desi
ABEC Indonesia Vol. 12 (2024): 12th Applied Business and Engineering Conference
Publisher : Politeknik Negeri Bengkalis

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Acclimatization is a critical stage in plant micropropagation. Many plantlets resulting from tissue culture cannotsurvive when acclimatized. External environmental conditions will affect the ability of acclimatized plants to survive. Apart from climatic conditions (temperature and sun intensity), the type of media and its composition of the planting mediacan also influence the ability of plantlets to survive and grow. This research aims to: 1) see effect of media plantingcomposition on acclimatization of fig micro shoot-tip cutting, and 2) find the best composition of acclimatization mediatreament for the success and growth of acclimatized fig from micro shoot-tip cutting. The research was carried out using aRandomized Block Design with 7 treatments, and each treatment was repeated 4 times. Each experimental unit was plantedwith 6 cuttings of fig plants resulting from in vitro multiplication. The data were subjected to analysis of variance, anddifferences between acts were tested with Tukey Test at the 5% level. The results showed: 1) The media plantingcomposition were success to influence acclimatization of fig micro shoot-tip cutting, 2) The best composition ofacclimatization media treament for the success and growth of fig micro shoot-tip cutting is top soil+cocopeat, and 3) Thehighest percentage of survival rate on fig micro shoot-tip cutting acclimatization (75%) obtained on top soil+cocopeatmedia followed by top soil media (45%).

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