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Phytochemical properties and antibacterial activity of green tea leaf extract from gunung gambir jember against Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans: an experimental study Meilawaty, Zahara; Ermawati, Tantin; Sari, Desi Sandra; Ningsih, Indah Yulia; Rachmawati, Dessy; Nulend, Jenneke Klein
Padjadjaran Journal of Dentistry Vol 36, No 3 (2024): November 2024
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol36no3.58316

ABSTRACTIntroduction: Green tea leaves are abundant in bioactive components, including catechins, flavonoids, and polyphenols. These compounds are known for their antibacterial, anti-inflammatory, and antioxidant properties. In addition, green tea also contains minerals, such as Calcium, Phosphor, Potassium, Magnesium, and Sulphur. However, the precise mineral content and anti-bacterial properties of green tea against Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans from Gunung (Mount) Gambir in Jember Regency are currently unknown. This study aimed to analyze the phytochemical composition and antibacterial activity of green tea leaf extract from Gunung Gambir, Jember against Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. Methods: The bioactive constituents of green tea leaf extract were identified using a UV-Vis spectrophotometer to measure its total flavonoid content (TFC) and total phenolic compounds (TPC). Mineral identification was performed using flame atomic absorption spectrometry. The disc diffusion method was used to assess antibacterial activity by quantifying the diameter of the inhibition zone in cultures of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans treated with green tea leaf extract at concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, and 100%. Anaerobic conditions were created by incubating the Petri dishes in a desiccator for 24 hours at 37°C. One-Way Anova test was used to analyze the data, and differences were considered statistically significant at p<0.05. Subsequently, a LSD test was carried out (p<0,05). Results: The TPC value was 1.607±0.742 mg GAE/g, while the TFC value was 99.146 ± 0.307 mg GAE/g. Calcium appeared to be the most abundant mineral in the tea extract, measured at 31.65 ppm. The inhibitory power of green tea leaves extract against both bacteria was not significantly different (p>0.05). Conclusion: Green tea leaves extract from Gunung Gambir Jember Regency in Indonesia is rich in phenolic compounds, flavonoids, and certain minerals, such us Phosphor, Calium, Magnesium, Sulphur and Calcium. These constituents are known to possess biological and antibacterial activity against Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans.KEYWORDS Antibacterial, flavonoid, green tea extract, minerals, phenol, Jember

Microbiological Profile in Oral Cavity Infection in Diabetic Rats with Periodontitis Ermawati, Tantin; Indriana, Tecky; Hamzah, Zahreni; Suhartini, Suhartini; Rachmawati, Dessy
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.60-71

Background: Diabetes melitus is a chronic metabolic disease due to the inability of the pancreas to produce insulin or the body cannot use insulin effectively.Periodontitis is one of the complications of microvascular disorders that ranks second in oral cavity diseases. One of the aerobic bacteria thought to play a role in the severity of diabetes melitus is Staphylococcus aureus, while the pathogenic anaerobic bacteria in periodontal disease are Treponema, Bacteroides, Porphyromonas, Prevotella, Capnocytophaga, Peptostreptococcus, Fusobacterium, Actinobacillus, and Eikenella. The study aimed to determine the microbiological profile in oral cavity infections of rats with diabetes melitus and periodontitisMethod: This study was experimental laboratories on male wistar rats induced by diabetes melitus and periodontitis using wire ligature on mandibular molars, which were divided into 3 treatment groups: group I (control), group II (diabetes melitus) and group III (diabetes melitus and periodontitis). Periodontitis (wire) rats were treated for 7 days and saliva was collected to identify the microbiological profile of the oral cavity. Result: Identification indicated the presence of genus and species of bacteria in the saliva of rats in 3 groups, gram positive: Staphylococcus intermedius, Staphylococcus cohnii subsp.urelyticus. Gram negative: Chromobacterium violaceum, Kleibseilla pneumoniae, Eikenella corrodens, Enterobacter sakazaki and Chryseobacterium meningosepticum. Conclusion: The number of bacterial colonies in the treatment group of diabetes melitus and periodontitis rats was greater than the treatment group of diabetes melitus rats and the control group. Gram staining results found groups of gram positive and gram negative bacteria.

Microbiological Profile in Oral Cavity Infection in Diabetic Rats with Periodontitis Ermawati, Tantin; Indriana, Tecky; Hamzah, Zahreni; Suhartini, Suhartini; Rachmawati, Dessy
Odonto : Dental Journal Vol 11, No 1 (2024): July 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.1.60-71

Background: Diabetes melitus is a chronic metabolic disease due to the inability of the pancreas to produce insulin or the body cannot use insulin effectively.Periodontitis is one of the complications of microvascular disorders that ranks second in oral cavity diseases. One of the aerobic bacteria thought to play a role in the severity of diabetes melitus is Staphylococcus aureus, while the pathogenic anaerobic bacteria in periodontal disease are Treponema, Bacteroides, Porphyromonas, Prevotella, Capnocytophaga, Peptostreptococcus, Fusobacterium, Actinobacillus, and Eikenella. The study aimed to determine the microbiological profile in oral cavity infections of rats with diabetes melitus and periodontitisMethod: This study was experimental laboratories on male wistar rats induced by diabetes melitus and periodontitis using wire ligature on mandibular molars, which were divided into 3 treatment groups: group I (control), group II (diabetes melitus) and group III (diabetes melitus and periodontitis). Periodontitis (wire) rats were treated for 7 days and saliva was collected to identify the microbiological profile of the oral cavity. Result: Identification indicated the presence of genus and species of bacteria in the saliva of rats in 3 groups, gram positive: Staphylococcus intermedius, Staphylococcus cohnii subsp.urelyticus. Gram negative: Chromobacterium violaceum, Kleibseilla pneumoniae, Eikenella corrodens, Enterobacter sakazaki and Chryseobacterium meningosepticum. Conclusion: The number of bacterial colonies in the treatment group of diabetes melitus and periodontitis rats was greater than the treatment group of diabetes melitus rats and the control group. Gram staining results found groups of gram positive and gram negative bacteria.

EFFECTIVENESS OF ROBUSTA COFFEE BEAN EXTRACT GEL ON COLLAGEN FIBERS DENSITY IN POST-GINGIVECTOMY WOUND HEALING Ermawati, Tantin; Harmono, Happy; Kartikasari, Dania
Odonto : Dental Journal Vol 8, No 1 (2021): July 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Gingivectomy is a periodontal surgical procedure that eliminates excess gingival tissues by excision, usually to remove a gingival pocket which leads to scars and recontours the gingival to its proper anatomical form. Wound healing is a process to restore the missing functions and structures of a tissue due to injuries or inflammations. Robusta coffee bean contains many bioactive compounds that can accelerate the wound healing process. The study aimed to determinate the effectiveness of robusta coffee bean extract gel with concentration 40%,50%,60% on collagen fibers density in post-gingivectomy wound healing Method: 36 Wistar rats were divided into 9 groups: control, gingivectomy without therapy, gingivectomy +40% robusta coffee bean extract gel, gingivectomy +50% robusta coffee bean extract gel, and gingivectomy+60% robusta coffee bean extract gel. The last 4 groups were divided again into day 3 and 7 euthanasia. The results were processed by Trichrome Mallory to visualized the collagen fibers and then measured by ImageJ software.Result: Robusta coffee beans extract gel therapy with concentration 40%, 50% and 60% had higher average collagen fibers density compared to group without therapy.Conclusion: Robusta coffee bean extract gel increased the density of collagen fibers in post-gingivectomy wound healing

EFFECTIVENESS OF ROBUSTA COFFEE BEAN EXTRACT GEL ON COLLAGEN FIBERS DENSITY IN POST-GINGIVECTOMY WOUND HEALING Ermawati, Tantin; Harmono, Happy; Kartikasari, Dania
Odonto : Dental Journal Vol 8, No 1 (2021): July 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.8.1.45-53

Background: Gingivectomy is a periodontal surgical procedure that eliminates excess gingival tissues by excision, usually to remove a gingival pocket which leads to scars and recontours the gingival to its proper anatomical form. Wound healing is a process to restore the missing functions and structures of a tissue due to injuries or inflammations. Robusta coffee bean contains many bioactive compounds that can accelerate the wound healing process. The study aimed to determinate the effectiveness of robusta coffee bean extract gel with concentration 40%,50%,60% on collagen fibers density in post-gingivectomy wound healing Method: 36 Wistar rats were divided into 9 groups: control, gingivectomy without therapy, gingivectomy +40% robusta coffee bean extract gel, gingivectomy +50% robusta coffee bean extract gel, and gingivectomy+60% robusta coffee bean extract gel. The last 4 groups were divided again into day 3 and 7 euthanasia. The results were processed by Trichrome Mallory to visualized the collagen fibers and then measured by ImageJ software.Result: Robusta coffee beans extract gel therapy with concentration 40%, 50% and 60% had higher average collagen fibers density compared to group without therapy.Conclusion: Robusta coffee bean extract gel increased the density of collagen fibers in post-gingivectomy wound healing

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