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All Journal Medula The Indonesian Biomedical Journal Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Acta Biochimica Indonesiana Poltekita : Jurnal Ilmu Kesehatan Jurnal Gema Ngabdi NURSING UPDATE JURNAL ILMIAH ILMU KEPERAWATAN JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Borneo Journal of Pharmacy

Tien Tien

Departemen Biokimia, Fakultas Kedokteran, Universitas Halu Oleo

Author-ID : 2638961

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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Pemanfaatan Lahan Tidur melalui Penanaman Nilam di Desa Kosambi Kecamatan Benua Kabupaten Konawe Selatan, Provinsi Sulawesi Tenggara Sapto Raharjo; Tien Tien; A Kadir La Ode
Jurnal Gema Ngabdi Vol. 2 No. 1 (2020): Jurnal Gema Ngabdi
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jgn.v2i1.79

Kosambi is a rural area, located in a high altitude area which belongs to Benua, South Konawe Regency, South East Sulawesi Province. The population is self-help transmigration from the province of South Sulawesi. Kosambi villagers generally burn and / or cut down forests to obtain land that will be used as planting areas. The land is not directly cultivated, but it is left first while collecting capital. This condition has the potential to cause erosion, and forest fires. Through the service funding scheme, the internal Community Partnership Program of Halu Oleo University in 2018 has successfully cultivated ¼ hectares of idle land from total of 2 hectares. Land management is performed through a partnership with patchouli farmers. At this initial stage, the land is only planted with patchouli. Patchouli planting patterns are arranged well so that the photosynthesis process occurs optimally. After six months, in August 2019, the first harvest was carried out. In the first harvest, 15 kg patchouli oil produced with a selling price of Rp. 505,000,- per kilogram. This year, cocoa planting will be tried as a protective crop in the new area planted with patchouli. Patchouli and cacao tree planting programs are expected to generate maximum farmer income in the future

Pengaruh Ekstrak Bawang Putih Terhadap Kadar Serum Kreatinin Tikus Hipertensi Two Kidney One Clipp (Effect of Allium sativum Extract to Serum Creatinine of Two Kidney One Clipp Hypertension Rat) Sapto Raharjo; Gustavita Maria Bandong; Tien Tien; Andi Noor Kholidha Syarif; Agus Chahyadi; Pranita Aritrina
MEDULA JURNAL ILMIAH FAKULLTAS KEDOKTERAN UNIVERSITAS HALU OLEO Vol 7, No 1 (2019)
Publisher : Halu Oleo University

Background: Garlic (Allium sativum) has long been used both as a food and traditional medicine. Several studies have shown potential of garlic (Allium sativum) as an antihypertensive. In previous in vitro study found that dipeptide on garlic extract after hydrolyzed by the enzyme papain have ACE inhibitory activity. Induction of hypertension with 2 Kidney One Clip (2K1C) methodcauses the kidneys lose their physiological functions. A.sativum is expected to ameliorate kidney function. This study aimed to analyze creatinine levels in rat induced by 2K1C. Method: These study was an in vivo study with Pre-Post Test Only Control group Design. Before treatment, 30 subjects induced hypertension by 2K1C method. Treatment was conducted for four weeks. The recent study was conducted on 36 Rattus norvegicus, Wistar strain were divided into 6 groups. Measurement of creatinine was conducted by spectrophotometry method. Result: The results showed that there were significant differences in creatinine levels between groups (p < 0.05) both in the pre-test and post-test. The normal group and hypertension group did not have significant differences in creatinine pre- and post-test levels (p > 0.05). A.sativum 75m/kgBB gave the best decrease in creatinine levels up to 1.75 mg/dL compared to the A.sativum 25mg/kgBB and A.sativum group 50 mg/kgBB. There were significant differences in creatinine levels A.sativum 75 mg/kgBB and captopril (p <0.05). Conclusion: There was significant differences in creatinine levels of hypertensive rats between groups and the most effective dose was 75 mg / kg. Keywords: A.sativum, hypertension, creatinine, 2K1C Latar Belakang: Bawang putih (Allium sativum) telah lama digunakan, baik sebagai bahan masakan maupun sebagai obat tradisional. Beberapa studi menunjukkan potensi bawang putih (A. sativum) sebagai antihipertensi. Pada penelitian sebelumnya secara in vitro ditemukan bahwa dipeptida pada ekstrak bawang putih setelah dihidrolisis dengan enzim papain memiliki aktivitas inhibisi terhadap ACE. Induksi hipertensi 2 Kidney One Clip (2K1C) menyebabkan ginjal kehilangan fungsi fisiologisnya. Pemberian A.sativum diketahui mampu memperbaiki fungsi ginjal. Penelitian ini bertujuan untuk menganalisis kadar kreatinin pada tikus yang diinduksi 2K1C. Metode: Penelitian ini merupakan jenis penelitian in vivo dengan menggunakan rancangan Control Group Time Series Experimental. Parameter dalam penelitian ini adalah tekanan darah. Sebelum perlakuan, subjek diinduksi hipertensi dengan metode 2K1C. Perlakuan dimulai pada minggu ke-enam setelah induksi. Perlakuan berlangsung selama empat minggu. Subjek yang digunakan dalam penelitian adalah tikus putih (Rattus norvegicus, strain Wistar), sebanyak 36 ekor dibagi dalam 6 kelompok: normal/sehat (tidak diinduksi), hipertensi yang diberi kaptopril, hipertensi yang diberi ekstrak A.sativum 25mg/kgBB, hipertensi yang diberi ekstrak A. sativum 50mg/kgBB, hipertensi yang diberi ekstrak A. sativum 75mg/kgBB, dan hipertensi tanpa perlakuan. Pengukuran kreatinin dilakukan menggunakan metode spektrofotometri. Hasil: Analisis statistik menunjukkan terdapat perbedaan kadar kreatinin signifkan pada setiap kelompok (p < 0.05) baik pre-test maupun post-test. Kelompok normal dan hipertensi tidak memiliki perbedaan signifikan pada kadar kreatinin pre dan post-test (p > 0.05). Kelompok A. sativum 75m/kgBB menunjukkan penurunan kadar kreatinin yang sangat signifikan hingga 1.75 mg/dL dibanding kelompok A. sativum 25mg/kgBB and A. sativum group 50 mg/kgBB. Terdapat perbedaan kadar kreatinin yang signifikan antara kelompok A. sativum 75 mg/kgBB dan captopril (p <0.05). Simpulan: Terdapat perbedaan kadar kreatinin yang signifikan antar kelompok dan penurunan kadar kreatinin terbaik yaitu pada dosis 75 mg / kg. Kata Kunci: A.sativum, hipertensi, kreatinin, 2K1C

Microbiological and Biochemical Contamination Analysis of Refilled Drinking-water in Abeli, Kendari, Southeast Sulawesi Juminten Saimin; Hartati Hartati; Yenti Purnamasari; Sufiah Asri Mulyawati; Tien Tien; Pranita Ayitrina
The Indonesian Biomedical Journal Vol 12, No 2 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i2.871

BACKGROUND: The access to safe drinkingwater is increasingly difficult, especially in water catchment or coastal areas. Due to the difficulty, people in coastal areas tend to consume ready-to-use refilled drinking-water. However, the sanitation facilities on most drinking-water refill depots do not meet the requirements, hence really prone to microbial contamination. We conducted this study to determine the types of bacteria causing the contamination of refilled drinking-water from refill depots in Abeli, a coastal area in Kendari.METHODS: Samples were collected from all 6 drinkingwater refill depots in Abeli. Three-series fermentation tubes were used for the microbiological test, including the presumptive, confirmative and completed tests. The biochemical test was performed with indole test, methyl red test, Voges-Proskauer test, and citrate utilization test (IMViC) methods and the triple sugar iron agar (TSIA) test.RESULTS: Among 6 refilled drinking-water samples, we found the growth of coliform bacterial colonies in 3 samples, exhibiting various characteristics. Based on the characteristics, both microbiological and biochemical, the identified bacteria were Proteus sp., Escherichia coli and Klebsiella oxytoca.CONCLUSION: Bacteria that contaminated refilled drinking-water in Abeli were Proteus sp., E. coli and K. oxytoca. Continuous supervision is required to ensure the quality of water from drinking-water refill depots.KEYWORDS: bacteria, Proteus sp., Escherichia coli, Klebsiella oxytoca, coastal areas, refilled drinking water

PERBANDINGAN INDEKS MASSA TUBUH DAN TEKANAN DARAH PADA PEKERJA DI RUMAH POTONG HEWAN DAN KELOMPOK PETANI DI KOTA KENDARI Dionisius Excelsis Deo Sombolinggi; Andi Noor Kholidha Syarifin; Tien Tien; Laode Kardin; Pranita Aritrina; Arimaswati Arimaswati
NURSING UPDATE : Jurnal Ilmiah Ilmu Keperawatan P-ISSN : 2085-5931 e-ISSN : 2623-2871 Vol 13 No 1 (2022): MARET
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36089/nu.v13i1.716

Circadian rhythm is a natural clock in the human body. Night work can cause disturbances in the circadian rhythm. Circadian rhythm disturbances are known to be associated with an increase in psychosocial stress which can lead to an increase in blood pressure and BMI. This study aimed to compare BMI and blood pressure in day and night workers. This study was an analytical observational methods with a design case control. It was conducted in Labibia Village, Mandonga District and Anggoya Slaughterhouse, Kendari City in November 2021. The number of samples is 65 respondents, consist of 20 slaughterhouse workers and 45 farmer groups. Data analysis was carried out with the statistical test Chi-Square and then continued by determining the Odds Ratio (OR). The results showed that there was no significant difference between BMI of slaughterhouse workers and farmer groups (p = 0,096; POR 2,471 95% CI 0,840 – 7,265) and there was a significant difference between blood pressure of workers in abattoirs and farmer groups (p = 0,000 ; POR 52,250 ; 95% CI 6,293 – 433,842). There was no significant difference between BMI in slaughterhouse workers and farmer groups in Kendari city. There was a significant difference between blood pressure in abattoir workers and farmer groups in Kendari city in 2021 and night work was a risk factor for increasing blood pressure.

In Vitro Anti-Inflammatory Activities of Ethanolic Extract Elephantopus Scaber Leaves Tomy Nurtamin; I Putu Sudayasa; Tien Tien
JKKI : Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 9, No 1, (2018)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol9.Iss1.art9

Background: Inflammation is a protective physiological response to tissue injury that can be caused by harmful stimuli. If the inflammatory process is prolonged and cannot restore to homeostatic conditions, this may lead to pathological effects that can damage cells and cause various diseases. Elephantopus scaber is a plant that can easily be found in Indonesia. Elephantopus scaber is a type of plant that is often used as a traditional medicines. Several studies have shown that the compound bioactive content contained in plants has enormous potential as alternative medicine.Objective: This present study was to investigate the anti-inflammatory activity of ethanolic extract of Elephantopus scabe leaves.Methods: The Elephantopus scaber leaves were extracted using ethanol solvent into different concentration (50 mg/mL, 100 mg/mL, and 120 mg/mL). Diclofenac sodium was used as the standard. Anti-inflammatory assays were performed by the human red blood cell (HRBC) membrane stabilization method and heat-induced hemolysis method. Phytochemical screening that used in the present study was a conventional method.Results: Phytochemical screening showed the presence of flavonoids, tannins, and saponins. In the present study, ethanolic extract of Elephantopus scaber leaves has anti inflammatory activity by protecting the stability of red blood cell membrane. The highest protection capability possessed by the ethanolic extract of Elephantopus scaber leaves in both human red blood cell (HRBC) membrane stabilization method and heatinduced hemolysis method was at a concentration of 100 mg/mL.Conclusion: The ethanolic extract of Elephantophus scaber has antiinflammatory activities by in vitro assays.

Pengaruh Extra Virgin Olive Oil (EVOO) Terhadap Kadar Lipid dan Indeks Massa Tubuh Wanita Obesitas Usia Produktif Ririn Afrianto; Tien Tien; Nina Indriyani; Tety Yuniarty Sudiro; Andi Noor Kholidha Syarifin; Haryati Haryati
Poltekita : Jurnal Ilmu Kesehatan Vol. 16 No. 2 (2022): August
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jik.v16i2.1153

Saat ini World Health Organization telah menetapkan obesitas sebagai epidemik global. Obesitas sering dihubungkan dengan kejadian dislipidemia termasuk penurunan kadar high density lipoprotein (HDL) dan peningkatan kadar low density lipoprotein (LDL). Hal ini juga sangat berkaitan dengan munculnya risiko penyakit jantung bahkan kematian. Pencarian bahan alami untuk memperbaiki status lipid pada penderita obesitas terus dilakukan, salah yang potensial dengan kandungan metabolitnya yang melimpah adalah minyak zaitun. Minyak zaitun mengandung asam lemak tak jenuh dan flavonoid yang dapat menurunkan lipotoksisitas dan ukuran sel lemak. Jenis minyak zaitun yang paling baik digunakan adalah extra virgin olive oil (EVOO), sehingga tujuan yang ingin dicapai dalam penelitian ini adalah untuk mengetahui perbedaan kadar lipid dan indeks masa tubuh (IMT) pada wanita obesitas usia produktif sebelum dan setelah pemberian EVOO. Penelitian ini merupakan studi quasi-eksperimental dengan pendekatan one group pretest-posttest without control group. EVOO dikonsumsi selama 3 minggu dengan dosis 30 mL/hari. Penelitian ini didapatkan rerata kadar HDL, LDL, dan IMT pada subjek sebelum dilakukan intervensi (pre-test) adalah 42,27 ± 4,5 mg/dL, 131,18 ± 16,9 mg/dL dan 28,4273 ± 3,3 kg/m2, sedangkan rerata kadar HDL, LDL, dan IMT pada subjek setelah dilakukan intervensi (post-test) EVOO adalah 44,09 ±4,6 mg/dL, 127,45 ± 29,4 mg/dL, dan 28,2164 ± 3,4 kg/m2. Analisis statistik menunjukkan tidak terdapat perbedaan yang bermakna antara kadar HDL, LDL, dan IMT sebelum dan setelah pemberian EVOO

Faktor Risiko Kejadian Ulkus Diabetik Pada Penderita Diabetes Mellitus Tipe 2 di RSUD Kabupaten Muna Arimaswati Arimaswati; Nina Indriyani Nasruddin; Agussalim Ali; Tien Tien; Pranita Aritrina; Adellya Febriyani Hadini; Adry Leonardy Tendean
NURSING UPDATE : Jurnal Ilmiah Ilmu Keperawatan P-ISSN : 2085-5931 e-ISSN : 2623-2871 Vol 13 No 4 (2022): DESEMBER
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36089/nu.v13i4.966

Diabetes mellitus (DM) is a non-communicable disease that has become a serious public health problem. Diabetic ulcers are a form of chronic complications of type 2 diabetes that often occur. Facto factors that influence the incidence of diabetic ulcers in people with type 2 diabetes are direct factors including age, gender, education, occupation, long suffering from diabetes, smoking, sports, use of footwear and irregular foot care. Meanwhile, indirect factors are family support in the form of emotional support, appreciation support, instrumental support, and alternative support. The purpose of this study was to determine the risk factors associated with the incidence of diabetic ulcers in type 2 diabetes mellitus sufferers at RSUD Kabupaten Muna. This research is a type of observational analytic using aapproach case control study. The case group was diabetic ulcer patients diagnosed by a doctor and recorded in the medical record, the control group was type 2 diabetes mellitus patients diagnosed by a doctor and recorded in the medical record. The number of samples was 66 samples consisting of 33 case groups and 33 control groups. The sampling technique usedmethod purposive sampling. The results of research at RSUD Kabupaten Muna showed the results of bivariate analysis of age showed values (OR: 11,200; CI 95%; 3,388-37,020), duration of DM ≥ 10 years showed a value (OR: 8,543; CI95%; 2,796-26,104) OR: 10,075; 95% CI; 2,052-49,469), hypertension showed a value (OR: 6,250; CI 95%; 2,131-18,330), GDS levels showed a value (OR: 9,000; CI95%; 2,870-28,224), sport showed a value ( OR: 14,062; 95% CI; 4,278-46,230), smoking showed a value (OR: 13,796; 95% CI; 4,238-44,910). Age, duration of diabetes mellitus ≥ 10 years, obesity, hypertension, GDS levels, sport, and smoking are risk factors associated with the incidence of diabetic ulcers in type 2 diabetes mellitus sufferers at RSUD Kabupaten Muna.

Uji Larvasida Ekstrak Etanol Buah Mengkudu (Morinda citrifolia L.) terhadap Jentik Nyamuk Aedes Aegypti Arimaswati Arimaswati; Sufiah Asri Mulyawati; Tien Tien; Pranita Aritrina; Nur Rahmi
NURSING UPDATE : Jurnal Ilmiah Ilmu Keperawatan P-ISSN : 2085-5931 e-ISSN : 2623-2871 Vol 13 No 3 (2022): SEPTEMBER
Publisher : NHM PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36089/nu.v13i3.968

Dengue Hemorrhagic Fever (DHF) is an endemic disease in Indonesia and includes acute viral infections from Flaviviridae family that are transmitted through intermediate host Aedes aegypti with symptoms of sudden high fever for two to seven days accompanied by bleeding. Noni fruit (Morinda citrifolia L.) contains active compounds as larvacide such as alkaloids, saponins, proxeronin and terpenoids. The purpose of this research is to know larvacide activity of ethanol extract of noni fruit to Aedes aegypti larvae death. This is an experimental research with post test only control group design. The sample used is 700 Aedes aegypti larvae which have reached III/IV instar and ethanol extract of noni fruit (Morinda citrifolia L.) divided into 5 concentrations, 0.5%, 1%, 2%, 4%, 8% and two control group namely negative control and positive control. Each treatment was repeated 4 times. The larvacide activity test was performed by observing the number of larvae deaths within 24 hours at 1 hour, 2 hour and 24 hour observation (5, 10, 15, 30, 60, 120, and 1440 min) and looking at the LC50 and LT50 values of the larvae tested. Result of this research shows that noni fruit (Morinda citrifolia L.) has a larvacide activity against the death of Aedes aegypti larvae. The Death of larvae was highest at 8% concentration with 100% death percentage in the 10 minutes and lowest at 0,5% concentration with 86% death percentage in the 1440 minutes. The probit analysis obtained LC50 in the 120 minutes is 1,44544% and LT50 at concentration of ethanol extract of noni fruit 2% is 70,8 minutes. From this research can be concluded that ethanol extract of noni fruit (Morinda citrifolia L.) has a larvacide activity to Aedes aegypti larvae death.

Inhibition of HMG-CoA Reductase Activity by Kersen Leaves (Muntingia calabura L.) to Prevent Hypercholesterolemia: Inhibisi HMG-CoA Reduktase Menggunakan Ekstrak Daun Kersen (Muntingia calabura L) Untuk Mencegah Hiperkolesterolemia Tien Tien; Nishar Rakhman Ardiansyah; Carla Wulandari Sabandar; Laode Kardin; Pranita Aritrina
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 9 No. 1 (2023): (March 2023)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/j24428744.2023.v9.i1.16086

Background: Hypercholesterolemia is a condition of total cholesterol level >200 mg/dL and LDL >130 mg/dL. HMG-CoA (3-hydroxy-3-ethylglutaryl-coenzyme A) reductase is an enzyme that has a role in cholesterol biosynthesis. Hence, inhibition of this enzyme led to the decrement of cholesterol level. The extract of Kersen leaves (Muntingia calabura L.) is known to contain flavonoids, terpenoids, steroids, tannins, phenolic, and alkaloids. Flavonoids work by inhibiting the HMG-CoA reductase activity, so that mevalonate cannot be formed and thus decrease the cholesterol synthesis. Objectives: The present study aimed to determine the effect of Kersen leaves extract (M. calabura L.) in inhibiting the HMG-CoA reductase activity in vitro. Material and Methods: The study is a true experimental study with a post-test-only control group design. The independent variables were ethanol, methanol, and n-hexane extracts of Kersen leaves. Moreover, the percentage inhibition of the enzyme was the dependent variable. The test was conducted in vitro using UV-Vis spectrophotometry with pravastatin as a positive control. Results: The inhibitory effects of ethanol, methanol, n-hexane extracts of Kersen leaves, and pravastatin towards HMG-CoA reductase activity were 85.56%, 59.75%, 92.03%, and 99.58%, respectively. Post Hoc One-Way ANOVA showed that the p-values of pravastatin with ethanol, methanol, and n-hexane extracts were 0.687, 0.048, and 0.931, respectively. The n-hexane and ethanol extracts were potent for inhibiting the enzyme activity (p>0.05) comparable to pravastatin. Conclusion: The n-hexane and ethanol extracts of Kersen leaves could serve as a natural source of HMG-CoA reductase inhibitor to prevent hypercholesterolemia.

Histopathological analysis of the liver in hypercholesterolemia rats treated with Dillenia serrata fruits Tien; Tri Wistya Utami; Pranita Aritrina; Laode Kardin; Sukurni; Andi Noor Kholidha Syarifin
Acta Biochimica Indonesiana Vol. 5 No. 2 (2022): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.56

Background: Dillenia serrata (locally known as singi) is a natural product with the potential to improve liver function in hypercholesterolemia, due to anticholesterol and antioxidant properties. Objective: This study aimed to investigate the effect of D. serrata on the histopathological features of rat livers induced by a high-fat diet. Methods: This study used a quasi-experimental in vivo with a post-test-only control group design. Rats were divided into four groups: normal, high-fat diet, as well as D. serrata, and simvastatin treatment. Hypercholesterolemia was induced by a high-fat diet for two weeks. Rat liver tissues were analyzed histologically using Hematoxylin-Eosin (HE) staining, and were observed under a light microscope at 40x magnification in five wide fields of view. Results: The high-fat diet group had the most adipose cells, while the D. serrata group had the least. Statistical analysis demonstrated a significant difference between the high-fat diet group and the other groups (p < 0.05). Interestingly, no significant difference between the D. serrata group and either the normal or simvastatin group (p > 0.05), suggesting the treatment of fruit may restore liver function comparable to normal and simvastatin group. Conclusion: The D. serrata fruit reduces the number of fat cells in the histopathology of rats induced with the high-fat diet.

Co-Authors A Kadir La Ode Adellya Febriyani Hadini Adry Leonardy Tendean Agus Chahyadi Agussalim Ali Ardiansyah Ardiansyah Arimaswti Arimaswati Carla Wulandari Sabandar Dionisius Excelsis Deo Sombolinggi Gustavita Maria Bandong Hartati Hartati Haryati Haryati I Putu Sudayasa Juminten Saimin Kamaruddin, Harni Sartika Laode Kardin Laode Kardin Laode Kardin Mulyawati, Sufiah Asri Nina Indriyani Nina Indriyani Nasruddin Nur Rahmi Pranita Aritrina Pranita Aritrina Pranita Aritrina Pranita Aritrina Pranita Ayitrina Raja Al fath Widya Iswara Rana Triana Amin Reskiya Nur Insani Ririn Afrianto Sapto Raharjo Sukurni Syarifin, Andi Noor Kholidha Tety Yuniarty Sudiro Tomy Nurtamin Tri Wistya Utami Yenti Purnamasari Zulkifli Zulkifli

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