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Antibacterial Activity of Endophytic Bacteria from Butterfly Pea (Clitoria ternatea L.) Root against Staphylococcus aureus and Escherichia coli Polandos, Chersa; Fitriani, Any
Bioeksperimen: Jurnal Penelitian Biologi March 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i1.10346

Abstract

Indonesia is rich in biodiversity, with many medicinal plants such as Clitoria ternatea (butterfly pea) known for antibacterial properties. This study aimed to evaluate the antibacterial activity of ethyl acetate extracts from endophytic bacteria isolated from C. ternatea roots, with potential application in developing natural antibacterial agents. The bacteria were identified as belonging to the genera Bacillus, Pseudomonas, and two strains of Azomonas (A and B). Extracts were tested against Staphylococcus aureus and Escherichia coli using Disk Diffusion Assay (DDA), Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Time-Kill assays. Results showed Pseudomonas extract at 40 mg/ml had the largest inhibition zone against S. aureus (6.75 ± 0.353 mm), while Azomonas A was most effective against E. coli (3.187 ± 0.618 mm). The lowest MIC against S. aureus was 2.5 mg/ml from Azomonas B, with MBCs of 20 mg/ml for Pseudomonas and Azomonas B. Against E. coli, Azomonas A showed MIC and MBC of 10 mg/ml and 40 mg/ml, respectively. Time-Kill assays confirmed bacterial reductions at MIC and higher concentrations. These findings suggest C. ternatea’s endophytic bacteria extracts have promising antibacterial potential for sustainable medicinal use.
The Effect of Banana Peel Biosorbent (Musa Acuminate) on Water Clarity, pH, TDS, and DO Levels in Household Wastewater in Bandung Fatharani, Aulia; Al-Wafi, Ok M Abthal; Marhamah, Yualinda Durotul; Shani, Nadiya Syafia; Fitriani, Any; Yulianty, Nurfitri
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Publisher : Universitas Pendidikan Indonesia and Konsorsium Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Banana peel consists of nitrogen atoms, sulfur and organic materials such as carboxylic acids that can function to bind pollutant molecules. The purpose of this research is to make King Banana (Musa acuminate) peel as filtration media to improve the physical quality of water including water clarity, pH value, TDS and Dissolved Oxygen (DO). There are three stages carried out, including preparation of banana peel waste, adjusting the media and filtration time and data analysis. The preparation process of banana peel waste is done by cleaning the banana peel then dried and mashed, then alkalized using 0.5 N NaOH. Then a variation in the amount of filtration media is made, namely: 60 g, 80 g and 100 g of plantain peels formulated using an additional 30 grams of zeolite with variations in filtration time, namely: 3 hours, 6 hours and 9 hours. The filtration results obtained using a variation of 100 grams of filtration media with a filtration time of 9 hours is the best media combination where the results obtained show an increase in water clarity, pH 5.41 and an increase in DO (Dissolve oxygen) of 20.