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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Neutrino : jurnal fisika dan aplikasinya El-Hayah : Jurnal Biologi Alchemy : Journal of Chemistry Majalah Obat Tradisional Journal of Islamic Pharmacy Journal of Islamic Medicine Journal of Research on Community Engagement
Avin Ainur Fitrianingsih
Universitas Islam Negeri Maulana Malik Ibrahim Malang
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences

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Cross Reaction among Antibody Pili sub unit Hemagglutinin Proteins and Outer Membrane sub unit Hemagglutinin Proteins of Shigella flexneri Avin Ainur Fitrianingsih; Lailia Nur Rachma; Alvi Milliana; Tinny Endang Hernowati; Aulanni'am Aulanni'am; Sanarto Santoso; Sumarno Reto Prawiro
Journal of Tropical Life Science Vol. 7 No. 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.01

Abstract

Shigella  flexneri is the most common causal agent of shigellosis. Its pili are composed of pili protein subunits. Adhesion molecules can be found on the pili and outer membrane proteins (Omp). A hemagglutination reaction can be used for screening of adhesion molecules. Objectives: The purpose of this study was to determine the molecular weight of the pili protein subunits and outer membrane proteins of S. flexneri that act as hemagglutinin proteins, and to prove whether there is a cross-reaction between antibodies against hemagglutinin pili protein subunits and outer membrane proteins of S. flexneri. Methods: Pili protein subunits were isolated using pili bacteria cutters, and the outer membrane proteins were solubilized and obtained using sodium dodecyl sulfate 0.05% as detergent for Omp isolation.  The hemagglutination reaction used mice erythrocytes. The cross reactions between subunit pili proteins were conducted by Western blot and Dot blot. Results:. Antibodies against hemagglutinin sub unit pili protein 18 kDa responded to pili protein subunits 18 kDa; 23 kDa; 34 kDa; and 53 kDa; and Omp 23 kDa and 27 kDa. Omp and subunit pili proteins S. flexneri consists of several identical epitopes  that were responsible for the similarity of the response profile in the cross-reactions of antibodies. 
NEUTRAL PH AND COLD TEMPERATURES STABILIZE THE PROTECTIVE EFFECT OF CRUDE PILI S. FLEXNERI ON THE ILEAL MUCOSA OF MICE Avin Ainur Fitrianingsih; Lailia Nur Rachma; Alvi Milliana
Jurnal Neutrino:Jurnal Fisika dan Aplikasinya Vol 12, No 1 (2019): October
Publisher : Department of Physics, Maulana Malik Ibrahim State Islamic University of Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/neu.v12i1.15525

Abstract

Alternative therapies are being sought as a result of the rising concerns with inadequate and untrustworthy medical treatments for Shigella flexneri. The current study aimed to assess the preventive and therapeutic effects of crude pili S. flexneri on of neutral pH and cold temperature against Shigella flexneri infection in immunocompetent mice. Twenty male Swiss albino mice were randomly assigned to one of two groups: control or experimental groups. Each group was subsequently separated into four equal subgroups. In order to infect the mice, 4 weeks before infection, the experimental subgroups were given crude pili S. flexneri orally in every week (three times) until the completion of the research. After following the treatment of crude pili S. flexneri, all group infected with Shigella flexneri. The small intestines of mice were processed and analyzed for the presence of the pathological lesions. Jejunal portions were measured. The findings revealed that vaccinated mice had a statistically significant increase in the quantity of health mucose as compared to non-infected group in ileal sections. Crude pili S. flexneri was administered to the intestinal portions of all subgroups before or after the infection, and the architecture was found to be more or less normal. Our data indicate that crude pili S. flexneri in neutral pH and cold temperature is a useful preventative and a potentially effective treatment medication for S. flexneri infection.
REACTION BETWEEN Shigella dysentriae SUBUNIT PILI ANTIGEN WITH 18 KDA Shigella flexneri SUBUNIT PILI ANTIBODY Avin Ainur Fitrianingsih, M. Biomed.
El-Hayah Vol 9, No 1 (2022): EL-HAYAH (VOL 9, NO 1 SEPTEMBER 2022)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v9i1.15621

Abstract

Shigellosis caused by S. flexneri and S. dysentriae is the leading cause of death in developing countries. Meanwhile, the development of the Shigella vaccine is still ongoing. This study aims to prove the reaction between the 18 kDa S. flexneri pili sub-unit protein's antibodies and with the S. dysentriae pili sub-unit protein antigen. This study used the Western Blot and Dot Blot tests to determine the antibody-antigen reaction both qualitatively and quantitatively.  From the study results, it was found that the 18 kDa S. flexneri 18 kDa pili subunit protein was able to recognize the S. dysentriae pili sub-unit protein with a molecular weight of 17 kDa and 23 kDa. The protein subunit pili S. dysentriae with a molecular weight of 17 kDa and 23 kDa probably has an epitope recognized by the antibody to the pili protein subunit S. flexneri 18 kDa. The equivalent zone of the antibody-antigen reaction lies in the concentration of the antibody protein subunit pili 18 kDa S. flexneri titer 1/300 and the concentration of the antigen protein subunit pili 18 kDa S. dysentriae titer 1/20. So that the higher the dilution of the antibody of the 18 kDa S. flexneri pili subunit, the lower the bond with the S. dysentriae pili subunit antigen.
Pengabdian kepada Masyarakat dengan Pendekatan Edukatif dan Strategi Kolaboratif Pada Situasi Pandemi Covid-19 di Dusun Krajan, Desa Sumbersekar, Kabupaten Malang Mardhiyah, Ainatul; Fitrianingsih, Avin Ainur
JRCE (Journal of Research on Community Engagement) Vol 2, No 2 (2021): Journal of Research on Community Engagement
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jrce.v2i2.10452

Abstract

Covid-19 has been declared a pandemic by WHO since March 11, 2020. The spread of this virus has reached 114 countries including Indonesia. Preventing the spread of Covid-19 is important to tackle this pandemic. Community service UIN Serving Qaryah Thayyibah is participating in socializing ways to prevent Covid-19. The theme of this activity is the socialization of Covid-19 prevention and the practice of making hand sanitizers according to WHO standards. The method used was education by distributing Covid-19 prevention pocket books and direct meetings with PKK cadres in Krajan Hamlet, Sumbersekar Village, Dau District, Malang Regency. The results of this activity were seen from the enthusiasm of the participants during the socialization activities and the practice of making hand sanitizers. It is hoped that from this activity, the people of Krajan Hamlet can better understand how to prevent the spread of Covid-19 and can make hand sanitizers according to WHO standards.
Co-Authors Abdul Malik Setiawan Adnani, Basyar Ahmad Riza Wahdi Ainatul Mardhiyah Alvi Milliana Astari, Lina Fitria Aulanni'am Aulanni'am Aulanni'am, Aulanni'am Fitri Purworini, Fitri Haris Nursyah Arifin, Haris Nursyah Lailia Nur Rachma Luky Adrianto Nabila Rahmadani Prawiro, Sumarno Reto Purnomo S, Fahrurrozi Hari Rachmawati Ningsih Riskiyana, Riskiyana Roihatul Mutiah Sanarto Santoso Sanarto Santoso Sumarno Reto Prawiro Tinny Endang Hernowati Tinny Endang Hernowati Yen Yen Ari Indrawijaya Zainabur Rahmah