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HANS-JOACHIM FREISLEBEN
Medical Research Unit, Faculty of Medicine, University of Indonesia
Medicine & Pharmacology

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20 years German-Indonesian Medical Association (GIMA) / Deutsch-Indonesische Gesellschaft für Medizin (DIGM e.V.) Bundschu, Hans-Dieter; Freisleben, Hans-Joachim
Medical Journal of Indonesia Vol 25, No 2 (2016): June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.868 KB) | DOI: 10.13181/mji.v25i2.1484

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[no abstract available]
International collaboration for implementation of equal access to family-based primary healthcare in Indonesia Haier, Jörg; Schäfers, Jürgen; Freisleben, Hans-Joachim
Medical Journal of Indonesia Vol 26, No 2 (2017): June
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.302 KB) | DOI: 10.13181/mji.v26i2.2103

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[No abstract available]
Reconsidering markers of oxidative stress Freisleben, Hans-Joachim
Medical Journal of Indonesia Vol 22, No 4 (2013): November
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.703 KB) | DOI: 10.13181/mji.v22i4.597

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The preventive effect of Mangifera foetida L. leaf extract administered simultaneously to excess iron on markers of iron overload in Spraque-Dawley rats Fajri, Purnama; Estuningtyas, Ari; Louisa, Melva; Freisleben, Hans-Joachim
Medical Journal of Indonesia Vol 26, No 4 (2017): December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (647.087 KB) | DOI: 10.13181/mji.v26i4.1829

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Background: Recently, there is no agent available for the prevention of iron overload (IO) in thalassemia patients. Previous studies showed that Mangifera foetida L. leaf extracts reduced the levels of iron in IO in vitro and in vivo models. The present study aimed to determine the efficacy of Mangifera foetida L. leaf extract in the prevention of IO induced in rats.Methods: Thirty male Sprague-Dawley rats were divided into 5 groups: control (untreated), IO, 3 treatment groups with leaf extract equivalent to 50, 100, and 200 mg of mangiferin per kg BW. Fe-dextran (15 mg) was administered intraperitoneally twice a week for 4 weeks to all groups except control (IO, DSM50, DSM100, and DSM200). Urine and blood samples were taken before and after treatments. After 4 weeks of treatment, rats were terminated, and samples of spleen, liver, and heart were taken. Ferritin and mangiferin levels and SOD activities were determined in plasma. Iron levels were measured in plasma, urine, and spleen.Results: Experimental IO increased plasma Fe content 4.23 times and plasma ferritin levels 6.9 times vs normal. Mangifera foetida L. leaf extract DSM50 resulted in the highest blood levels of 212 ng mangiferin per mL and moderately, although not significant, prevented increased plasma ferritin levels and IO in organs and protected against oxidative stress.Conclusion: Aqueous Mangifera foetida L. leaf extract may be useful to prevent IO and oxidative stress in thalassemia patients.
Stable archaeal tetraether lipid liposomes for photodynamic application: transfer of carboxyfluorescein to cultured T84 tumor cells Oertl, Anton; Antonopoulos, Emmanouil; Freisleben, Seruni U.; Freisleben, Hans-Joachim
Medical Journal of Indonesia Vol 25, No 4 (2016): December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (944.858 KB) | DOI: 10.13181/mji.v25i4.1506

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Background: Archaeal membranes have phytanyl ether lipids instead of common fatty acid-glycerol esters in bacterial and eukaryotic cells. Sulfolobus and Thermoplasma species have unique membrane-spanning tetraether lipids (TEL), which form stable liposomes. Recently, we cultured Thermoplasma species from the Indonesian volcano Tangkuban Perahu and isolated TEL. The purpose of this in vitro study is to investigate the transfer of fluorescent dye from stable TEL liposomes to cultured colon carcinoma cells.Methods: TEL was extracted from cultured cells with chloroform-methanol (1:1), then it was fractionated and purified via diethylaminoethyl-cellulose-acetate columns and activated charcoal for the formation of stable liposomes. For the fluorescence exchange assay, TEL liposomes were loaded with water-soluble carboxyfluorescein (CF). Staining experiments were conducted with various cell cultures, and T84 colon carcinoma cells were chosen for the main experiments. Liposome stability was tested by light scattering and electron microscopic size determinations as well as by unspecific CF release at low pH (6.0–7.4) and increased temperature  (4–50°C/70°C).Results: TEL liposomes exhibit high stability and extremely low proton permeability at low pH. CF staining of cultured T84 colon carcinoma cells appeares more intensive from TEL liposomes than from dipalmitoylphosphatidylcholine liposomes.Conclusion: The results of this in vitro study demonstrate CF staining of colon carcinoma cells and high stability of TEL liposomes at low pH, matching the condition in the gastro-intestinal (GI) route and in the urogentital (UG) tract. For this reason, in vivo studies on liposomal fluorescent photosensitizers for topical application of photodynamic cancer therapy in the GI and UG tracts should be carried out.
Archaeosomes and Tetraether Lipid Liposome Freisleben, Hans-Joachim
Majalah Ilmu Kefarmasian Vol. 9, No. 1
Publisher : UI Scholars Hub

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Archaea have phytanyl ether lipids which is one of the characteristics that separates them from bacteria and eukaryotes. Some archaea have also unique membrane spanning tetrae ther lipids (T EL); in Sulfolobus and Ihermoplasma species these TELmake up the majority of total membrane lipids. Archaeal lipids are able to form stable liposomal structures, both from membrane factions, mainly the polar membrane fraction, i.e. archaeosomes, or from highly purified TEL (tetraether lipid liposomes). Liposomes of the main polar lipid (MPL) from thermoacidophilic archaeon Ihermoplasma acidophilum were thoroughly inevstigated. Archaeosomes and TEL liposomes exhibit extremely low proton permeability and high stability at low pH, which makes them suitable to delivery of therapeutics and vaccines via the gastro-inetestinal route. Liposomes from egg lecithin can be stabilized by incorporation of 11-12 mole% MPL. MPL liposomes show long-term shelf stability even at high temperatures without conservation. The size of MPL liposomes between 100 nm (unilamellar) and several gm (multilamellar) depends mainly on the method of preparation. Toxicity and mutagenicity have not been detected in toxicological screening. This is a review report on the lipid from archaea to be developed as an alternative to conventional liposome for various industrial applications, especially for pharmaceutical industry.
Co-Authors A.A. Ketut Agung Cahyawan W Alida Harahap Ani R. Prijanti Antonopoulos, Emmanouil Ari Estuningtyas, Ari Bethy S. Hernowo Budi Kurniawan Bundschu, Hans-Dieter David Tandian Fajri, Purnama Frans Ferdinal Freisleben, Seruni U. Haier, Jörg Isnani A.S. Suryono Jenny Hidayat Krista Kuebel-Thiel Melva Louisa Mohamad Sadikin Mohammad A. Aulia Na P. Bo Nia Yuliatri Oertl, Anton Pustika A. Wahidiyat Rahmawati Ridwan Renindra A. Aman Rondang R. Soegianto Rostika Flora Schäfers, Jürgen Septelia I. Wanandi Seruni K.U. Freisleben Soehardjo Poertadji Sri Handayani Syaiful Ichwan Tiwuk Susantiningsih