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All Journal VALENSI Prosiding Pendidikan Sains Jurnal Riset Kimia Jurnal Kimia dan Kemasan Jurnal Zarah BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Chimica et Natura Acta Prosiding Seminar Nasional Pakar JURNAL PHOTON Jurnal Katalisator Journal of Midwifery and Nursing Science Midwifery Lumbung Farmasi : Jurnal Ilmu Kefarmasian Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Jurnal Kimia dan Kemasan Rafflesia Journal of Natural and Applied Sciences (RJNAS)
Zulkarnain Chaidir
Andalas University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Environmental Science Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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ISOLASI DAN SKRINING MIKROALGA AIR TAWAR SEBAGAI SUMBER PIGMEN KAROTENOID Arif Juliari Kusnanda; Bayu Afnovandra Perdana; Abdi Dharma; Zulkarnain Chaidir
Jurnal Kimia dan Kemasan Vol. 43 No. 1 April 2021
Publisher : Balai Besar Kimia dan Kemasan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24817/jkk.v43i1.6827

Abstract

Mikroalga menjadi salah satu sumber senyawa aktif yang memiliki potensi sebagai sumber pangan fungsional sebagai antioksidan seperti karotenoid. Penelitian ini bertujuan untuk mengisolasi mikroalga dari sumber perairan air tawar dan melakukan penapisan terhadap mikroalga sebagi sumber karotenoid. Mikroalga diisolasi dengan menggunakan mikropipet dan dikultivasi dalam media Bold’s Basald Medium (BBM). Penentuan tingkat pertumbuhan dengan spektrofotometer UV-Vis, penentuan berat biomassa kering secara gravimetri, skrining total karotenoid dilakukan menggunakan metode Lichtenthaller dengan spektrofotometer UV-Vis. Berdasarkan hasil penelitian diperoleh empat spesies mikroalga dengan warna hijau yang menandakan semuanya merupakan golongan mikroalga chlorophyta (alga hijau). Skirining total karotenoid dilakukan terhadap empat spesies hasil isolasi dan 2 spesies mikroalga dari Laboratorium Biokimia yaitu Scenedesmus rubescens dan Galdiera sulphuraria. Hasil skrining kandungan total karotenoid tertinggi adalah mikroalga hasil isolasi dari air kolam dengan yang merupakan genus chlorophyta sebesar 36±0,25 μg/g. Hasil ini memberikan informasi isolat mikroalga hasil isolasi dapat dijadikan sebagai mikroalga potensial sebagai sumber dan produksi karotenoid.  
UJI SENYAWA ANTIMIKROBA DARI ASAM LEMAK DAN FATTY ACID METHYL ESTER (FAME) MIKROALGA Nannochloropsis oculata Zulkarnain Chaidir; Sari Rahmi; Marniati Salim
Prosiding Seminar Nasional Pakar Prosiding Seminar Nasional Pakar 2019 Buku I
Publisher : Lembaga Penelitian Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25105/pakar.v0i0.4144

Abstract

Microalgae contains many bioactive molecules including lipid that can be useful for development of antimicrobial compounds. The aim of this research is to test antimicrobial compounds from fatty acid and fatty acid methyl ester (FAME)from microalgae Nannochloropsis oculata. Lipid from microalgae was extracted using hexane and transesterfied using methanol dan sulfuric acid p.a as catalist. Antimicrobial activities were tested using disc diffusion methode against Staphylococcus aureus, Escherichia coli, and yeast Candida albicans, at varying concentrations are 100, 200, and 300 mg/L. The result shown that fatty acids and FAME from microalgae Nannochloropsis oculata have antibacterial activity and do not have anti-candidal activity against Candida albicans. Antibacterial activity of fatty acids is higher than antibacterial activity of FAME on both bacteria. The result shown that fatty acids and FAME contained in lipid of microalgae Nannochloropsis oculata are palmitic acid, stearic acid, methyl lauric, and methyl stearic.
MEMPELAJARI DAYA INHIBISI ION CU2+ DAN CA2+ SERTA POLA INHIBISINYA TERHADAP ENZIM AKONITASE YANG DIISOLASI DARI BUAH KALIKIH ALANG (RICINUS COMMUNIS) Riryn Novianty; Zulkarnain Chaidir; Elida Mardiah
Sistem Informasi Vol 5 No 1 (2014): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v5i1.411

Abstract

Penelitian tentang inhibisi aktivitas enzim akonitase dengan menggunakan inhibitor ion Cu2+ dan Ca2+ telah dilakukan. Enzim akonitase diisolasi dari buah Ricinus communis dengan menggunakan aseton dingin. Untuk menentukan aktivitas dari akonitase digunakan metoda pengukuran substrat asam sitrat sisa berdasarkan metoda LStahre’s test. Kadar protein ditentukan dengan menggunakan metoda Lowry. Optimasi dari enzim akonitase didapatkan dari hasil pH optimum 7,3, temperatur optimum 40 oC, dan konsentrasi substrat 1 %. Dari kurva Lineaweaver Burk diperoleh harga Km = 0,104 M. Sedangkan untuk mengetahui daya pola inhibisi ion Cu2+ dan Ca2+ dilakukan dengan menvariasikan konsentrasi dari masing-masing ion tersebut. Daya inhibisi ion Cu2+ dengan konsentrasi 0,1 M adalah 93,347 % dan daya ion Ca2+ pada konsentrasi 0,1 M adalah 60,124 %. Hasil tersebut menunjukkan pola inhibisi yang sama yakni inhibisi non kompetitif dan masing-masing ion memiliki daya inhibisi yang berbeda.
The Antibacterial Activity Test Comparison of Green and Black Tea Ethanol Extract (Camellia sinensis) Against Propionibacterium acnes Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
Science Midwifery Vol 10 No 2 (2022): April: Science Midwifery
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v10i2.544

Abstract

A study was conducted in connection with the comparison of antibacterial activity tests between ethanol extracts from green tea leaves and black tea leaves (Camellia sinensis) against Propionibacterium acnes. There were obtained by a softening technique that was softened for 24 hours. The filtrate was then concentrated to give a concentrated extract and evaporated ethanol content. The concentrated extract was then diluted with distilled water to extract 5%, 10%, and 15% concentration variations. The test method for inhibitory activity was the disc method using distilled water as a negative blank. The results obtained with blank, 5%, 10% and 15% green tea were 0. 5.25; 7.05 and 7.95 mm and black tea ethanol extract were 0; 2.50; 3.20 and 5.30 mm. The conclusion of this study is that the concentration of the extract is directly proportional to its inhibitory power, and the green tea ethanol extract is more potent than the black tea ethanol extract, so both can be used as antibacterial agents against P. acnes.
GREEN TEA ETHANOL EXTRACT EFFICACY AGAINST PSEUDOMONAS AERUGINOSA Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 9, No 1 (2022): August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i1.7336

Abstract

Research about green tea ethanol extract efficacy against Pseudomonas aeruginosa has been done. The green tea leaves were sourced from tea distributor in Sidamanik District, North Sumatra, Indonesia. Green tea leaves extract was obtained by maceration technique in which green tea leaves were soaked with ethanol 96% for 24 hours. After that, the filtrate was concentrated to be thick extract with no liquid content again.  After that, the concentrated extract was made by 5%, 10% and 15% as extract variations. The efficacy of green tea ethanol extract against Pseudomonas aeruginosa  was signed by increasing the inhibitory diameter that using disc method, in which the distilled water was used as negative blank. The results obtained for blanks, 5%, 10% and 15% extracts were 0; 1.85; 2.9 and 4.45 mm. The conclusion of this study is that the concentration of the extract is increasing proportionally to its inhibitory power, the higher of extract concentration is higher of its inhibitory activity against Pseudomonas aeruginosa. The conclusion described the green tea ethanol extract was effective to be antibacterial agent against Pseudomonas aeruginosa.
Isolasi dan Karakterisasi Bakteri Asam Laktat dari Teh Hijau Fermentasi Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
Lumbung Farmasi: Jurnal Ilmu Kefarmasian Vol 3, No 2 (2022): Juli
Publisher : UNIVERSITAS MUHAMMADIYAH MATARAM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/lf.v3i2.9645

Abstract

ABSTRAKTeh hijau adalah jenis teh yang tidak mengalami proses fermentasi akan tetapi mengalami proses pengeringan dan penguapan daun. Teh hijau memiliki aktivitas antioksidan yang tinggi karena kandungan katekin di dalamnya. Namun bioavaibilitas katekin teh hijau berkurang pada saluran cerna karena kurang stabil. Kehadiran probiotik pada teh hijau diharapkan mampu meningkatkan bioavaibilitasnya. Bakteri Asam Laktat (BAL) merupakan kelompok bakteri baik yang merupakan probiotik yang berperan dalam menyehatkan saluran cerna. Peneltian ini bertujuan untuk mengisolasi dan menguji karakterisasi BAL dari Teh Hijau Fermentasi. Teh hijau yang berasal dari Distributor Teh Juma, Kecamatan Sidamanik difermentasi dengan gula aren dan madu hutan dengan variasi waktu 24, 48 dan 72 jam pada suhu rendah. Teh hijau fermentasi kemudian diisolasi kemudian diencerkan dengan pengenceran 10-5 dan 10-6. Hasil inkubasi selama 48 jam diperoleh kemudian dimurnikan dengan metode streak dan diinkubasi kembali selama 24 jam kemudian diuji karakterisasinya secara morfologi, pewarnaan gram, uji katalase dan uji fermentasi. Hasil yang diperoleh adalah pada uji morfologi positif BAL dengan ciri-ciri terlihat koloni tunggal dengan bentuk bulat, licin, warna putih kekuningan.dan terbentuk zona bening di sekitar koloni. Hasil pewarnaan positif BAL dengan ciri-ciri bakteri gram positif ditunjukkkan oleh warna biru ke ungu. Hasil uji katalase ditunjukkan dengan ciri-ciri  tidak menghasilkan gelembung udara yang menandakan adanya BAL karena BAL tidak mengandung oksigen serta uji fermentasi yang ditunjukkan dengan tidak terbentuknya gelembung udara pada tabung Durham yang menandakan tipe fermentasi BAL adalah homofermentatif. Kata kunci : Isolasi; Karakterisasi; Bakteri asam laktat; Teh hijau; Fermentasi.ABSTRACTGreen tea is a type of tea that does not undergo a fermentation process but undergoes a process of drying and evaporation of the leaves. Green tea has high antioxidant activity due to the catechin content in it. However, the bioavailability of green tea catechins is reduced in the gastrointestinal tract because it is less stable. The presence of probiotics in green tea is expected to increase its bioavailability. Lactic Acid Bacteria (LAB) is a group of good bacteria which are probiotics that play a role in healthy gastrointestinal tract. This study aims to isolate and test the characterization of LAB from Fermented Green Tea. Green tea from Juma Tea Distributor, Sidamanik District was fermented with palm sugar and forest honey with variations of 24, 48 and 72 hours at low temperature. The fermented green tea was isolated and then diluted with 10-5 and 10-6 dilutions. The results of incubation for 48 hours were obtained and then purified by streak method and incubated again for 24 hours and then tested for morphological characterization, gram staining, catalase test and fermentation test. The results obtained were a positive morphological test of LAB with the characteristics of a single colony being seen with a round, smooth, yellowish white color, and a clear zone was formed around the colony. The results of positive LAB staining with gram-positive bacteria are indicated by a blue to purple color. The results of the catalase test are indicated by the characteristics of not producing air bubbles which indicate the presence of LAB because LAB does not contain oxygen and the fermentation test is indicated by the absence of air bubbles in the Durham tube which indicates the type of LAB fermentation is homofermentative. Keywords : Isolation; Characterization; Lactic acid bacteria; Green tea; Fermentation;
GREEN TEA ETHANOL EXTRACT EFFICACY AGAINST PSEUDOMONAS AERUGINOSA Aliyah Fahmi; Sumaryati Syukur; Zulkarnain Chaidir; Sri Melia
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 9 No. 1 (2022): August 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v9i1.7336

Abstract

Research about green tea ethanol extract efficacy against Pseudomonas aeruginosa has been done. The green tea leaves were sourced from tea distributor in Sidamanik District, North Sumatra, Indonesia. Green tea leaves extract was obtained by maceration technique in which green tea leaves were soaked with ethanol 96% for 24 hours. After that, the filtrate was concentrated to be thick extract with no liquid content again.  After that, the concentrated extract was made by 5%, 10% and 15% as extract variations. The efficacy of green tea ethanol extract against Pseudomonas aeruginosa  was signed by increasing the inhibitory diameter that using disc method, in which the distilled water was used as negative blank. The results obtained for blanks, 5%, 10% and 15% extracts were 0; 1.85; 2.9 and 4.45 mm. The conclusion of this study is that the concentration of the extract is increasing proportionally to its inhibitory power, the higher of extract concentration is higher of its inhibitory activity against Pseudomonas aeruginosa. The conclusion described the green tea ethanol extract was effective to be antibacterial agent against Pseudomonas aeruginosa.
Pelatihan Praktikum Kimia Sederhana di SMAN 16 Padang Imelda Imelda; Refinel Refinel; Refilda Refilda; Yefrida Yefrida; Hermansyah Aziz; Yulizar Yusuf; Suryati Suryati; Zulkarnain Chaidir; Sumaryati Syukur; Rahmiana Zein; Novesar Jamarun; Safni Safni; Zilfa Zilfa; Yetria Rilda; Norman Ferdinal; Tommi Hermansyah; Fivi Mona Bareno
Warta Pengabdian Andalas Vol 30 No 3 (2023)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.30.3.562-568.2023

Abstract

Chemistry is one of the subjects taught in State Senior High Schools (SMAN), especially in Natural Sciences (IPA). The problem with chemistry subjects is that it is difficult to practice in the laboratory because laboratory facilities such as space, equipment and chemicals are expensive. As a result, chemical laboratory practices were only sometimes carried out. Several lecturers and students from the Department of Chemistry, FMIPA Unand Kimia Unand, carried out service activities at SMAN 16 Padang through laboratory practice using materials and equipment that were cheap and readily available in the surrounding environment. This community service aims to train teachers and students to carry out chemistry practicums using simple materials and equipment available around them. The training was given to chemistry subject teachers and students in the science laboratory at SMAN 16 on 17 and 21 November 2022. The material was practised in oxidation and reduction (redox) and electrolysis reactions. The activity went smoothly; the students enthusiastically carried out the practicum. Through the results of this simple chemistry practicum, students can design and carry out chemical experiments using simple tools and materials, conduct direct observations of chemical processes, practice scientific thinking, attitude and work skills and analyze and solve various scientific processes using scientific methods. SMAN 16 hopes that this activity will continue with different topics.
Integrasi Bioremediasi Limbah Peternakan Sapi dan Kultivasi Mikroalga Chlorella vulgaris dan Chlorella pyrenoidosae Afriyanti Azhar; Abdi Dharma; Armaini armaini; Zulkarnain Chaidir; Syafriza Yanti; Nasril Nasir
Jurnal Katalisator Vol 2, No 2 (2017): KATALISATOR
Publisher : LLDIKTI Wilayah X

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.006 KB) | DOI: 10.22216/jk.v2i2.2127

Abstract

Dairy manure that functioned as organic fertilizer has potential impact as environmental destructive waste because the absorption of inorganic nutrients in the fertilizer. On the other hand, microalgae growth is hindered by the high cost of culture, whereas organic and inorganic nutrient requirements. Dairy manure have the potential for microalgae nutrients, but are hindered by turbidity levels. Its has been tested for dilution dairy manure and treatments on fluorescence and sunlight sources and the addition of urea commercial fertilizer to absorbs organic and inorganic nutrients. Its prove the integration of bioremediation of dairy manure and microalgae cultivation Chlorella pyrenoidosa and Chlorella vulgaris can be performed and also serves as a source of caratenoid (chlorophyll)
Desain Primer Dan Deteksi Gen CHS (chalcone synthase) Pada Tanaman Gambir (Uncaria gambir (Hunter) Roxb.) Tipe Riau Gadang Epi Supri Wardi; Sumaryati Syukur; Zulkarnain Chaidir; Jamsari Jamsari; Diza Sartika
RAFFLESIA JOURNAL OF NATURAL AND APPLIED SCIENCES Vol. 1 No. 1 (2021): April
Publisher : UNIB Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/rjna.v1i1.15591

Abstract

Gambir merupakan salah satu tanaman yang mengandung katekin.Kandungan katekin pada gambir merupakan komponen yang menjadi syarat utama dalam penentuan mutu gambir. CHS (Chalcone synthase) adalah salah satu gen yang terlibat di dalam proses biosintesis pembentukan katekin. Penelitian ini bertujuan untuk mendapatkan primer yang dapat digunakan dalam deteksi gen CHS (Chalcone synthase) pada tanaman gambir tipe riau gadang serta untuk melihat kemampuan primer yang telah didesain untuk mendeteksi gen CHS pada tanaman gambir. Pendesainan primer dilakukan dengan mengalignment 21 data sekuengen CHS dan dipilih daerah yang memiliki kesamaan basa antara sekuen gen yang dialignment untuk mendapatkan primer.  Isolasi DNA gen CHS tanaman gambir menggunakan metode CTAB dan untuk isolasi RNA menggunakan Total RNA Mini Kit (Plant) dari Geneaid. Sintesis cDNA menggunakan kit Rever Tra Ace® qPCR RT Master Mix with gDNA Remover (Toyobo).Pada hasil desain primer didapatkan empat primer forward dan satu primer reverse. Hasil desain primer yang dapat digunakan untuk deteksi gen CHS dengan hasil cDNA tanaman gambir yaitu primer F3-R1(TNGTCTTCTGCACNACCTCCGGNG - CCANTC CAASCCYTCWCCDGTSGT). Proses deteksi gen CHS pada daun gambir menghasilkan produk dengan estimasi sebesar 724 bp
Co-Authors Abdi Dharma Afriyanti Azhar Agung Abadi Kiswandono Arif Juliari Kusnanda Armaini - Bayu Afnovandra Perdana Edison Munaf Edison Munaf Elida Mardiah fahmi, Aliyah - Fivi Mona Bareno Girsang, Ermi Hasanah, Qomariah Hermansyah Aziz Hermansyah Aziz Hevira, Linda Imelda Imelda Iolantri Handra Jamsari Jamsari Marniati Salim Melia, Sri Melia, Sri Nasril Nasir Norman Ferdinal Novesar Jamarun Novianty, Riryn Putri Ramadhani Putri Ramadhani, Putri Qomariah Hasanah Rahmiana Zein Refilda Refilda Refinel - Safni Safni Sari Rahmi Sartika, Diza Sumaryati Syukur Suryati Suryati Syafriza Yanti Syafrizayanti, Syafrizayanti Syiffa Fauzia Tommi Hermansyah Wardi, Epi Supri Yefrida Yefrida Yetria Rilda Yulizar Yusuf Yunazar Manjang Yunazar Manjang Zilfa Zilfa