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All Journal PHARMACON Agrikultura Gontor AGROTECH Science Journal Jurnal Agro JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Panrita Abdi - Jurnal Pengabdian pada Masyarakat JURNAL GALUNG TROPIKA Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Agrotek Tropika Jurnal Agrotech Abditani : Jurnal Pengabdian Masyarakat AGROVITAL : Jurnal Ilmu Pertanian Agropet Agrica: Journal of Sustainable Dryland Agriculture BERNAS: Jurnal Pengabdian Kepada Masyarakat Biofarm : Jurnal Ilmiah Pertanian JAGROS : Jurnal Agroteknologi dan Sains (Journal of Agrotechnology Science) Media Pertanian AGRIC
Sri Sudewi
Alkhairaat University
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Journal : PHARMACON

 1 
UJI AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BUAH MANGGIS (GARCINIA MANGOSTANA L.) TERHADAP BAKTERI KLEBSIELLA PNEUMONLAE Melkianus, Brando; Fatimawali, Fatimawali; Sudewi, Sri
PHARMACON Vol 8, No 1 (2019): Pharmacon
Publisher : PHARMACON

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK KULIT BUAH MANGGIS (Garcinia mangostana L.) TERHADAP BAKTERI Klebsiella pneumonlaeBrando Melkianus1), Fatimawali1), Sri Sudewi1)1)PS. Framasi FMIPA Unsrat Manado, 95115ABSTRACTResearch has been carried out on the Antibacterial Activity Test of Mangosteen (Garcinia mangostana L.) Fruit Bark Extract against Klebsiella pneumoniae bacteria. This study aims to determine the antibacterial activity of mangosteen peel extract using the diffusion method with positive control of Ciprofloxacin and negative control of aquades and the test solution used by mangosteen peel extract. The result showed differences in extract concentrations of 25% (6.66 mm) categorized as medium, extract concentractions of 50% (8.83 mm) categorized as medium, extract concentractions of 75% (9.16 mm) categorized as medium, and extract concentractions of 100% (10.16 mm) categorized as strong in influencing the inhibition of the growth of Klebsiella pneumoniae bacteria. The result of this study also showed that mangosteen peel extract (Garcinia mangostana L.), has a broad-spectrum bioactive compound which means it can inhibit the growth of Gram negative bacteria.Keywords : Garcinia mangostana L, Klebsiella pneumoniae, CiprofloxacinABSTRAKTelah dilakukan penelitian tentang Uji Aktivitas Antibakteri Ekstrak Kulit Buah Manggis (Garcinia Mangostana L.) Terhadap Bakteri Klebsiella Pneumoniae. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak kulit buah manggis menggunakan metode difusi agar dengan kontrol positif Ciprofloxacin dan kontrol negatif aquades serta larutan uji yang digunakan ekstrak kulit buah manggis. Hasil penelitian menunjukkan Perbedaan konsentrasi ekstrak 25% (6,66 mm) termasuk sedang, konsentrasi ekstrak 50% (8,83 mm) termasuk sedang, konsentrasi ekstrak 75% (9,16 mm) termasuk sedang, dan konsentrasi ekstrak 100% (10,16 mm) termasuk kuat dalam mempengaruhi penghambatan pertumbuhan bakteri Klebsiella Pneumoniae. Hasil penelitian ini juga menunjukan bahwa Ekstrak kulit buah manggis (Garcinia Mangostana L.), memiliki senyawa bioaktif dengan spektrum luas artinya dapat menghambat pertumbuhan bakteri Gram negatif.Kata kunci : My Garcinia Mangostana L, Klebsiella Pneunoniae, Ciprofloxacin 
ISOLASI DAN UJI AKTIVITAS ANTIBAKTERI DARI BAKTERI YANG BERASOSIASI DENGAN ALGA TURBINARIA ORNATA (TURNER) J. AGARDH SERTA IDENTIFIKASI SECARA BIOKIMIA. Kosasi, Cicilia; Lolo, Widya; Sudewi, Sri
PHARMACON Vol 8, No 2 (2019)
Publisher : PHARMACON

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ABSTRACT Algae are a place to live for various microorganisms? symbiosis with them, and some of them were known to be used as antibacterial. This study aims to determine the antibacterial activity of associated bacteria isolates from Turbinaria ornata algae obtained from the Bay of Manado against Staphylococcus aureus and Escherichia coli bacteria. In the course of testing the isolation and purification of symbiotic bacteria from Turbinaria ornata algae, then antibacterial activity was testing using agar diffusion method, then gram staining and biochemical tests were carried out including motility test,  tets, carbohydrate fermentation test, citrate test, lysine test, indole test and catalase test. The result showed  that there were 3 bacterial isolates, which had antibacterial activity in medium category against S.aureus and E.coli bacteria. Based on the results of biochemical identification, the isolates that have the greatest inhibitory power, they were thought to belong to genus Bacillus and the genus Yersinia.Keywords : Antibacterial, Symbionic bacteria, Turbinaria ornata (Turner) J.   Agardh. ABSTRAK Alga merupakan tempat hidup berbagai mikroorganisme yang bersimbiosis dengannya, dan beberapa dari spesies alga diketahui sering dimanfaatkan salah satunya  sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri dari isolat bakteri yang berasosiasi dengan alga Turbinaria ornata yang diperoleh dari Teluk Manado terhadap bakteri  Staphylococcus aureus dan Escherichia coli. Dalam pengujian dilakukan isolasi dan purfifikasi bakteri simbion dari alga Turbinari ornata, kemudian dilakukan pengujian aktivitas antibakteri menggunakan metode difusi agar, selanjutnya silakukan pewarnaan gram dan uji biokimia meliputi uji motilitas, uji H2S, uji fermentasi karbohidrat, uji sitrat, uji lisin, uji indol dan uji katalase. Hasil penelitian menunjukkan bahwa terdapat 3 isolat bakteri yang mempunyai daya aktivitas antibakteri dalam kategori sedang terhadap bakteri S. aureus dan E. coli. Berdasarkan hasil identifikasi secara biokimia isolat yang mempunyai daya hambat paling besar diduga termasuk dalam genus Bacillus dan genus Yersinia. Kata kunci : Antibakteri, Bakteri simbion, Turbinaria ornata (Turner) J. Agardh.
Co-Authors A. Nurfitriani Abdul Rahim Saleh Ade Rosmana Ambo Ala Ambo Ala Arfan Arfan Baharuddin Patandjengi Bisinda, Sultan Dewi Hs, Endang Sri Fatimawali . Hasmari Noer Idris Idris Idris If'all, If'all Jaya, Kasman Karmila Soar Kasman Jaya Kosasi, Cicilia Lisa Indriani B Lisa Indriani Bangkele Lisa Indriani Bangkele Marjun Marliyah, Marliyah Mar’atus Fitra Melkianus, Brando Mihwan Sataral Muh Farid BDR Muh. Farid B. D. R. Mumfahida Mumfahida Nurapiah Nurapiah, Nurapiah RATNAWATI Ratnawati Ratnawati Ratnawati Ratnawati Renaldi renaldi Sabariyah, Sitti Sayani Sayani , Sayani sayani Sitti Hardiyanti Sumarni Sumarni SYLVIA SJAM Taufik Hidayat Taufik Hidayat Tiara, Tiara Untung Surapati Tresnaputra Vevi Rosiani Widya Astuty Lolo, Widya Astuty