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All Journal Science and Technology Indonesia Lantanida Journal Narra J Jurnal Natural Heca Journal of Applied Sciences
Binawati Ginting
Universitas Syiah Kuala
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health

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Journal : Jurnal Natural

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Antioxidant ativity of dichloromethane extract from cocoa pod husk (Theobroma cacao L.) FEBRINA, RESA VERNIA; YAHYA, MUSTANIR; GINTING, BINAWATI; SAIDI, NURDIN
Jurnal Natural Volume 24 Number 2, June 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v24i2.36174

Abstract

Theobroma cacao L. is a plant that is reported to have strong antioxidant potential. A study was carried out to determine the antioxidant activity of dichloromethane extract of cocoa pod husk using 1,1-diphenyl-2-picrylhydrazyl (DPPH). The cocoa pod husk was macerated using n-hexane, ammonia-dichloromethane, and methanol. The TCD extract of cocoa pod husk showed the lowest IC50 value of 14.59 0.54 ppm based on the DPPH inhibition assay. The dichloromethane extract was isolated by gravity column chromatography with Silica gel G60 as the stationary phase andn-hexane: ethyl acetate as the mobile phase (gradient elution system). Fractions with identical staining patterns were combined into six fractions (TCD 1-TCD 6). The TCD 6 showed the lowest IC50 value of 127.05 0.54 ppm according to the DPPH inhibition assay. Based on the TLC results, TCD 4 was further isolated and four fractions (TCD 4.1 to TCD 4.4) were obtained. The TCD 4.4 had the lowest IC50 value (38.66 0.96 ppm).The TCD extract showed the presence of the dominant compound, -sitosterol, as determined by GC-MS analysis.
The validation of urea determination in saliva using optical urea biosensor with p-dimethylaminobenzaldehyde (DMAB) reagent ALDIANSYAH, TEUKU; SAFITRI, EKA; GINTING, BINAWATI; NAZARUDDIN, NAZARUDDIN; SUHUD, KHAIRI; SALEHA, SITTI; NURHAYATI, NURHAYATI; NAZARUDDIN, SALSABILLA LATANSA; AFIFI, MUHAMMAD RIDHO; NURHAIDA, NURHAIDA
Jurnal Natural Volume 25 Number 1, February 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v25i1.41251

Abstract

The validation of urea determination in saliva using the biosensor method and the p-dimethylaminobenzaldehyde (DMAB) method has been successfully conducted. Anthocyanin compounds from Chatarantus roseus flowers were extracted using the maceration method with methanol as the solvent, yielding a total of 22.60% with a total concentration of 6.01 mg/L. The obtained extract was subjected to qualitative anthocyanin testing, showing a positive result was indicated by the formation of a reddish color and quantitative testing with the formation of a faded yellow color. The maximum wavelength ( max) of anthocyanin was 664 nm with an absorbance of 0.674. The saliva samples were obtained from three different ages i.e: children, teenagers, and adults. During the saliva collection procedure, participants were instructed to abstain from consuming any food or beverages, with the exception of water, for a minimum of 1 hour prior to sample collection. All samples were stored in a freezer at 4-8C until needed for analysis. Linearity test results were assessed using a calibration curve, yielding a coefficient of determination (R) of 0.978 with a sensitivity of 0.033 for the biosensor and an R of 0.975 with a sensitivity of 0.685 for DMAB. The limit of detection (LOD) values were determined as 7.203 10 M for the biosensor and 6.984 10 M for DMAB, while the limit of quantification (LOQ) values were 2.182 10 M and 2.116 10 M, respectively. Statistical analysis using a t-test showed tcalculated = 1.314, compared to ttable = 4.302, indicating that tcalculated ttable. This result suggests no statistically significant difference between the biosensor and DMAB methods, confirming that the biosensor method is comparable in performance to the DMAB method. Additionally, urea concentration measurements showed that adult samples exhibited the highest urea levels among the tested samples, which may indicate an association with potential health risks, including dental caries, kidney failure, and liver damage.
Co-Authors - Fakhrurrazi Afifi, Muhammad Ridho ALDIANSYAH, TEUKU Bakhtiar , Bakhtiar Destiana, Khaerunisa Dewi Karlina Rusly Djufri - Eka Safitri Febrina, Resa Vernia Hakim, Rachmi F. Hamdiyati, Hamdiyati Hanafiah, Olivia A. Indriaty, Indriaty Kartini Hasballah Kemala, Pati Maghfirah, Ruhul Maidiani, Tika Marlina Marlina Murniana Murniana Muslem Muslem, Muslem Mutia Farida, Mutia N. Nazaruddin NAZARUDDIN, SALSABILLA LATANSA Nurdin Saidi Nurhaida Nurhaida Nurhayati Nurhayati Purnama, Agnia Putra, Noviandi I. Rahmazani, Shinta Rampengan, Derren DCH Rinaldi Idroes Romadhoni, Yenni Shafira, Ghina A. Sitti Saleha, Sitti Suhud, Khairi Yahya, Mustanir