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All Journal Buletin Peternakan Jurnal Ilmu Ternak Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Caraka Tani: Journal of Sustainable Agriculture JURNAL ILMIAH PETERNAKAN TERPADU Buletin Peternakan Tropis (Bulletin of Tropical Animal Science) Jurnal Kedokteran Hewan
Sigit Bintara
Faculty Of Animal Science, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Evaluasi Penggunaan Etanol dan Surfaktan Tween 80 dalam Melarutkan Vitamin E pada Bahan Pengencer Sperma Andromed Abdillah, Labib; Bintara, Sigit; Maharani, Dyah; Budisatria, I Gede Suparta
Buletin Peternakan Tropis Vol. 2 No. 2 (2021)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/bpt.2.2.125-129

Abstract

Penelitian ini dirancang untuk menguji bioavailabilitas vitamin E yang dilarutkan dengan menggunakan pelarut yang berbeda, yaitu surfaktan tween 80 dan etanol pada bahan pengencer semen Andromed. Perlakuan yang diberikan pada penelitian ini adalah bahan antioksidan vitamin E dan bahan pelarut minyak yang terbagi dalam kelompok Andromed kontrol (P0), Andromed  Vit E (0.2 gr) + etanol (P1), Andromed vit.E (0.2 gr) + tween 80 (P2), Andromed vit. E (0.4 gr) + etanol (P3) dan Andromed Vit. E (0.4 gr) + tween 80 (P4). Metode penelitian menggunakan pendekatan eksperimental, dan dianalisis secara deskriptif. Vitamin E dikombinasikan dengan pelarut sesuai perlakuan yang selanjutnya disebut sediaan vitamin E. Sediaan vitamin E kemudian dicampurkan kedalam bahan pengencer.  Sampel bahan pengencer tersebut dianalisis menggunakan analisis DPPH untuk mengukur aktivitas dari vitamin E di dalam bahan pengencer semen. Hasil penelitian menunjukkan nilai aktivitas antioksidan bahan pengencer yang diberikan kombinasi Vit. E dan tween 80 paling tinggi sebesar 91.29% sedangkan bahan pengencer dengan kombinasi Vit. E dan etanol aktivitas antioksidan tertingginya sebesar 57.45% dan hasil paling rendah pada bahan pengencer control tanpa penambahan vitamin E. Tween 80 sebagai pelarut vitamin E memiliki kemampuan yang lebih baik dalam melarutkan vitamin E dalam bahan pengencer semen jika dibandingkan dengan etanol.
ESTROGEN HORMONE PROFILE AND ESTRUS RESPONSE OF THIN TAILED EWES SYNCHRONIZED WITH CONTROLLED INTERNAL DRUG RELEASE Kumala, Seraphina; Asmarawati, widya; Ismaya, Ismaya; bintara, sigit; Aji, Riyan Nugroho; Widayati, Diah Tri
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i3.18583

Abstract

This research was conducted to identify the estrogen hormone profile and estrus response in the thin tailed ewes synchronized with controlled internal drug release (CIDR) implant. This research was carried out by employing 8 thin tailed ewes, CIDR, and estrogen kit. The ewes were distributed into: 3 ewes as control group and 5 ewes as treated group (with 12 days-CIDR implantation). Ewes were raised in the Sidomukti farm group, Sleman Regency, Yogyakarta. The data obtained were estrogen hormone profile during estrus examined by using ELISA method and estrus response marked with the reddening and oedema of vulva, mucus vaginal discharge, behavioral changes, vaginal pH, and superficial cells population. The data were analyzed by applying independent sample T-test. The result did not show any significant difference (P0.05) in the normal range between the control and CIDR group in estrogen hormone profile, estrus response, and pH level, but showed significant differences with positive correlation (P0.05) in population of superficial cells. In conclusion, CIDR was an effective tool for estrus synchronization at the farmer group, and gave positive estrus response towards thin tailed ewes with estrogen hormone profile, pH level, and superficial cell percentage on the normal range.
Regulation of Reactive Oxygen Species by Sodium Selenite in AndroMed® Extender Enhances Post-Thaw Saanen Buck Semen Quality Erlindra, Caterina Palma; Diatmono, Dio Fico Felsidan; Padmawati, Fransisca Gani; Sastranegara, Arina Dinar Nurfadilla; Sitaresmi, Pradita Iustitia; Bintara, Sigit; Widayati, Diah Tri
Caraka Tani: Journal of Sustainable Agriculture Vol 41, No 1 (2026): January
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v41i1.108422

Abstract

Semen cryopreservation is known to cause considerable damage to spermatozoa through the generation of free radicals. This damage can potentially be mitigated by the use of potent antioxidants, such as sodium selenite, which does not impair semen quality when administered in small doses. This study aimed to assess the impact of low-dose sodium selenite supplementation on post-thaw semen quality in Saanen bucks. Semen was collected from 2 superior Saanen bucks and evaluated according to standard criteria before dilution and freezing. Cryopreserved semen was stored in liquid nitrogen (LN2) at a temperature of -196 °C for 90 days. The experimental treatments involved the addition of sodium selenite to the AndroMed® diluent medium at 3 different concentrations: without supplementation/control (C), 5 ppm (T1), and 10 ppm (T2). Frozen semen was thawed at 37 °C in a water bath for 30 seconds before post-thaw quality assessment. The parameters assessed included motility, viability, abnormalities, plasma membrane integrity (PMI), acrosome membrane integrity (AMI), and reactive oxygen species (ROS). The data were statistically analyzed using One-Way analysis of variance (ANOVA). The results showed that the addition of sodium selenite had a significant effect (p < 0.05) on motility, viability, PMI, AMI, and ROS production, but did not affect the abnormality (p > 0.05). Interestingly, T1 resulted in the most substantial improvements, yielding the highest percentages of post-thaw motility (71.81±1.05%), viability (73.36±1.08%), PMI (77.49±1.68%), and AMI (79.29±0.63%), as well as the lowest ROS production (13.67±0.50%). In conclusion, the addition of 5 ppm sodium selenite enhances post-thaw semen quality in Saanen bucks.
Co-Authors Achadiah Rachmawati Ajat Sudrajat Aji, Riyan Nugroho Aji, Riyan Nugroho Akhmad Fathoni Ali Agus Ali Agus Ali Agus Andriyani Astuti Budi Prasetyo Widyobroto Diah Tri Widayati Diah Tri Widayati Diahanvika Tri Sarvinda Diatmono, Dio Fico Felsidan Dwi Astuti Dyah Maharani Eka Rizky Vury Rahayu Endang Baliarti Erlindra, Caterina Palma Fatimah Az Zahra Chairunissa Heru Sasongko I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria Ikhwan Firhamsah Ismaya (Ismaya) Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Kumala, Seraphina Kustantinah Kustantinah Kustono (Kustono) Labib Abdillah, Labib Luis Tavares Moh Takdir Nurul Hidayat Padmawati, Fransisca Gani Prajaningrum Luluk Miftahul Umami Priyadi, Dwi Ahmad Raden Febrianto Chsristi Rian Rosartio Riyan Nugroho Aji Sastranegara, Arina Dinar Nurfadilla Seraphina Kumala Sitaresmi, Pradita Iustitia Sri Harimurti Tety Hartatik Tri Anggraeni Kusumastuti Widya Asmarawati Yanuar Achadri Yudi Adinata Yuni Suranindyah