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All Journal Buletin Peternakan Jurnal Ilmu Ternak Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) JURNAL ILMIAH PETERNAKAN TERPADU Buletin Peternakan Tropis (Bulletin of Tropical Animal Science) Jurnal Kedokteran Hewan
Sigit Bintara
Faculty Of Animal Science, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Veterinary Other

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REPRODUCTIVE PERFORMANCES OF SUMBA ONGOLE COWS INSEMINATED WITH FROZEN BELGIAN BLUE SEMEN Riyan Nugroho Aji; Panjono Panjono; Ali Agus; Budi Prasetyo Widyobroto; Tety Hartatik; I Gede Suparta Budisatria; Ismaya Ismaya; Sigit Bintara
Buletin Peternakan Vol 41, No 4 (2017): BULETIN PETERNAKAN VOL. 41 (4) NOVEMBER 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i4.22052

Abstract

The purpose of this study was to observe the reproductive performances of Sumba Ongole (SO) cows inseminated with frozen Belgian Blue (BB) semen. This research was expected to be used as a reference in the cow’s maintenance, especially in the implementation of Artificial Insemination (AI), as well as for subsequent studies. Parameter of this study includes the signs of estrus symptoms, S/C ratio, CR, NRR, and pregnancy time. The results showed a large value of S/C for cow SO 2.60±1.81, great value for cattle CR SO was 40%, the value of NRR was 60%, and the pregnancy time SO was 273.80±4.08. It is concluded that reproductive performance of SO cows are good, using SO cows to cross with BB cow is possible to do. 
SRY Gene Marker Differences in Native and Crossbreed Cattle Tety Hartatik; Dwi Ahmad Priyadi; Ali Agus; Sigit Bintara; I Gede Suparta Budisatria; Panjono Panjono; Budi Prasetyo Widyobroto; Yudi Adinata
Buletin Peternakan Vol 42, No 3 (2018): BULETIN PETERNAKAN VOL. 42 (3) AUGUST 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i3.29940

Abstract

This study focused on the promoter region of the SRY gene with 1,281 bp DNA fragments, including 5'UTR, CAAT signal, and TATA box. Genomic samples of 19 cattle were obtained from Wagyu-BX (n = 2), BX (n = 5), Simmental (n = 2), Limousin (n = 2), Ongole (n = 2), Madura (n = 2), Bali (n = 2), Nellore (n = 1), and Hereford (n = 1). Two flanking primers (forward and reverse) were used for polymerase chain reaction (PCR). The PCR products were then sequenced by using a two-way primer. The obtained sequences were aligned with clustalW software to determine the differences in the nucleotide base arrangement which compiled the promoter region of the SRY gene. The cattle crossbreeding was done as an effort to improve the genetic variations and qualities. The SRY gene is a marker gene inherited from the male side (bull), so the SRY gene is expected to be used as a marker to monitor the crossbreeding. The monitoring of the crossbreed cattle is an initial effort to increase the genetic variations and enhance the genetic qualities without threatening the germplasm purity. The results of this study showed that the overall sample is monomorphic, except for Bali and Nellore cattle. Further research is needed by expanding the analysis area of the SRY gene and increasing the number of samples.
The Effect of Age on the Quality of Semen Turkeys (Meleagris gallopavo) Riyan Nugroho Aji; Ismaya Ismaya; Sri Harimurti; Heru Sasongko; Sigit Bintara; Widya Asmarawati
Buletin Peternakan Vol 42, No 4 (2018): BULETIN PETERNAKAN VOL. 42 (4) NOVEMBER 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i4.31372

Abstract

The purpose of this study was to observe the macroscopic and microscopic quality of Bronze turkey fresh semen on various ages. The observed toms were at the ages of 9 to 10 months (P1), 13 to 14 months (P2), and 17 to 18 months (P3), with each taken from 3 different toms. The collection was done by abdominal massage, and collected for 3 times a week in triplicate. This data was analyzed by analysis of variance (ANOVA) in a completely randomized design, followed by Duncan's New Multiple Range Test (DMRT) if there was any difference. The results showed that the average volume of turkey fresh semen were P1 (0.16 ± 0.04 ml), P2 (0.13 ± 0.02 ml), and P3 (0.10 ± 0.02 ml). The average pH of fresh semen were P1 (7.19 ± 0.05), P2 (7.12 ± 0.10), and P3 (7.06 ± 0.06). The average motility percentage of fresh semen were P1 (78.11 ± 1.38%), P2 (82.55 ± 1.17%), and P3 (74.55 ± 4.16%). The average viability percentage of fresh semen were P1 (78.63 ± 1.55%), P2 (83.26 ± 1.54%), and P3 (79.20 ± 1.06%). The average abnormal percentage of spermatozoa were P1 (3.01 ± 0.04%), P2 (3.04 ± 0.88%), and P3 (3.21 ± 0.44%). In conclusion, the best fresh semen quality is found on toms aged from 13 to14 months (P2). Furthermore, at the age 17 to18 months and more, the semen quality was decreased. 
Morphometry of Male Reproductive Organ and Sperm Evaluation of Belgian Blue Crossbreed Cattle as a Candidate of Superior Bull Riyan Nugroho Aji; Panjono Panjono; Ali Agus; Budi Prasetyo Widyobroto; Tety Hartatik; I Gede Suparta Budisatria; Ismaya Ismaya; Akhmad Fathoni; Dwi Astuti; Sigit Bintara
Buletin Peternakan Vol 44, No 4 (2020): BULETIN PETERNAKAN VOL. 44 (4) NOVEMBER 2020
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v44i4.45944

Abstract

The purpose of this study was to observe the size of reproductive organ and to evaluate the sperm quality of Belgian Blue (BB) crossbreed cattle. This cattle are the crossbreeding result between a female Brahman Cross (BX) cattle inseminated with frozen semen from pure male BB cattle, by Artificial Insemination (IB) method, as a candidate of superior bull. The samples of this study were 2.5 years old (BB1), (BB2), and (BB3) crossbreed cattle. Its process involved general physical examination, genital organ inspection, sperm production, and quality assessment. The data obtained were analyzed through descriptive analysis. The results of this study were the three BB crossbred cattle showed the good appearance of normal reproductive organs with scrotum circumferences of each bull were 40.2 cm, 38.6 cm, and 39.8 cm respectively, and sperm quality of the good and the normal motility values of each bull were 82%, 81% and 80%, viability 84%, 83%, and 86%, and the sperm concentrations of each bull were 2.218 million/ml, 1.986 million/ml and 2.120 million/ml respectively. As a result, it could be concluded that those three BB crossbreed cattle are suitable for being used as a superior bull.
Effect of Different Pre-Freezing Time on Quality of Frozen Fat-Tailed Ram Semen Sigit Bintara; Andriyani Astuti; Panjono Panjono; Tri Anggraeni Kusumastuti
Buletin Peternakan Vol 45, No 3 (2021): BULETIN PETERNAKAN VOL. 45 (3) AUGUST 2021
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v45i3.65426

Abstract

This study aimed to determining the effect of temperature changes on the sperm quality of fat-tailed sheep during the freezing process using a microcontroller. This study was conducted from April to October 2018 at the Laboratory of Animal Physiology and Reproduction, Faculty of Animal Science UGM. The study was used twelve mature ram. The methods were conducted by collecting semen using artificial vagina, semen dilution, freezing semen, thawing and semen quality test. The pre-freezing temperature time were grouped for 6, 9, and 12 minutes. Data of frozen semen quality (motility, viability, and abnormality) the data were analyzed using one-way ANOVA and the study was arranged using Completely Randomized Design (CRD). The average of spermatozoa motility after semen freezing with a 5 to -140°C decrease in pre freezing temperature for 12 minutes (50±5.3%) was significantly different (P<0.05) compared to 9 minutes (48±4.8%) and 6 minutes (43±4.8%). The average of spermatozoa viability after semen freezing with a decrease in pre freezing temperature of 5 to -140°C for 12 minutes (55±4.7%) was significantly different (P<0.05) compared to that for 9 minutes (52±3.5%) and 6 minutes (49±5.7%). The average of spermatozoa abnormality after freezing with a decrease in pre freezing temperature of 5 to -140°C for 6, 9, and 12 minutes was not significantly different (10±2.4%, 9±0.8%, and 10±0.9%, respectively). Based on the findings, it is possible to conclude that semen freezing at a lower pre-freezing temperature of 0 to -140°C for 12 minutes can improve the quality of freezing results.
Single Nucleotide Polymorphism of Partial GDF9 Gene in Three Local Goat of Indonesia Compare with Several Goat in Asia Fatimah Az Zahra Chairunissa; Sigit Bintara; Tety Hartatik
Buletin Peternakan Vol 46, No 1 (2022): BULETIN PETERNAKAN VOL. 46 (1) FEBRUARY 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i1.67188

Abstract

The GDF9 gene is a gene that affects the maturation of oocytes. GDF9 is expressed in oocytes and granulosa cell, it can stimulate granulose cell proliferation and regulate cumulus cell function from pre-ovulation to ovulation. The GDF9 gene is associated with an increase in the ovulation rate and litter size in animals. This study aims to determine the kinship relationship of local goats compared to goats in Asia on prolific traits and to determine the restriction mapping of the GDF9 gene in goats based on the different SNP locations. The local goat comes from the Bligon goat, Kacang goat and Kejobong goats which is compared to the GenBank data (EF446168, EU883989 and KY780296). GDF9 sequences were analyzed using BioEdit and sequencing results to identify Single Nucleotide Polymorphism (SNP) and using NEBCutter V2 to determine the restriction enzyme which recognized the sequence around SNP. The result shows that three variations of SNP were found in exon 2 (g.3615T>C, g.3760T>C and g.3855A>C). Identification of SNP position found 1 SNP position identified by restriction enzyme at g.3855A> C. The identified restriction enzyme is HpaII and MspI. The results of this study are expected to provide genetic information that will be used for further research on the relationship between GDF9 gene polymorphisms to animal prolific.
The Effect of Flushing Premating with Spirulina Platensis Supplementation on Ewes Postpartum Estrus Diahanvika Tri Sarvinda; Sigit Bintara; I Gede Suparta Budisatria; Kustantinah Kustantinah; Endang Baliarti
Buletin Peternakan Vol 46, No 1 (2022): BULETIN PETERNAKAN VOL. 46 (1) FEBRUARY 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i1.67523

Abstract

Lactating ewes require high nutrients for basic life requirements and milk production. If not fulfilled, it can have an impact on Negative Energy Balance (NEB) that reduces body weight, Body Condition Score (BCS), and extend the appearance of Postpartum Estrus (PPE). Premating flushing feed is an effort to improve ewe nutrients by adding high nutrition for preparation before mating so that after lambing and suckling, the ewe immediately estrus. This research aimed to evaluate the performance of ewes through premating flushing feeding. The research was conducted at Mendo Galak Farm, Sleman, Yogyakarta. Twenty ewes 2-3 years old with BCS 2-3 divided into two groups; the group with flushing treatment consisted of dried water spinach (Ipomoea reptans poir), concentrate feed with Spirulina sp. (14,92% crude protein, 60,28% total digestible nutrients), and the control group (PS) without Spirulina sp. (crude protein 11,82%, total digestible nutrients 53,20%). Flushing feed was given after a month postpartum as much as 3% dry matter of body weight. The recorded parameter was daily feed consumption, monthly body weight, BCS, and postpartum estrus. The data obtained were tested by an independent T-test with Statistical Product and Service Solution (SPPS ver. 22). The results showed the consumption and digestibility of CP, TDN, and ewe's ADG had a significant difference (P<0.05).  Postpartum estrus (PPE) of flushed ewes had no significant difference (P>0.05), 73.90±11.55 vs. 77.60±14.65 days, respectively. The conclusion was that flushing premating treatment with the addition of Spirulina platensis increased the nutrient intake and digestibility of CP, TDN, and ADG but had not shortened on postpartum estrus of lactating ewes.
The Effect of Thawing Duration on the Post Thawing Quality of Bali Cattle’s Frozen Semen and Conseption Rate in Smallholder Farms of East Lombok Regency Ikhwan Firhamsah; Sigit Bintara; Diah Tri Widayati
Buletin Peternakan Vol 46, No 2 (2022): BULETIN PETERNAKAN VOL. 46 (2) MAY 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i2.71035

Abstract

This research aimed to identify the effect of the length of thawing time on the quality  of  Bali  cattle postthaw semen and  to  investigate  the resulting pregnancy  rate. This  research  was  conducted  at  Wanasaba  Village,  specifically  Tanaq  Mira  Village, Wanasaba  District,  East  Lombok  Regency.  Five  samples  of  Bali  cattle  semen  were  collected  for  insemination  from  each  of  three  different  farmer  groups.  The  semen sample  from  the  remaining  frozen  semen  inseminated  by  the  inseminator  was  used  in this research. Tanaq Mira Village's inseminator performed thawing during the trip from the  artificial  insemination  (AI)  station  to  the  farmer  group's  location.  This  research observed  the  microscopic  quality  of  the postthaw frozen  semen  covering  motility, viability, and abnormality. Moreover, the pregnancy rate on AI acceptors using the non return  rate (NRR)  parameter  or  the  number  of  female parents who returned to  estrus after being inseminated were observed. The observation was conducted on three farmer groups  with  different  distances  and  lengths  of  thawing  time.  Each  farmer  group  was observed five times. The collected data were then analysed by using one-way analysis of variance(ANOVA). The research indicated that the distance of the farmer group’s location significantly affected (P<0,01) the sperm motility and viability. However, it did not significantly affect (P>0,05) the sperm abnormalities. The Sapeng farmer group had the  highest NRR, as  much  as 80%. According  to the  NRR  score,  it  can  be  concluded that the thawing process  using  the  thermos  within  less  than 10 minutes  resulted  in  the best yield.
The Effect of Lycopene Addition on the Semen Quality of Saanen Goats Stored at 5°C for 24 hours Sigit Bintara; Diah Tri Widayati; Widya Asmarawati; Riyan Nugroho Aji
JURNAL ILMIAH PETERNAKAN TERPADU Vol 11, No 1 (2023)
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v11i1.p34-40

Abstract

In order to increase the livestock population, a good reproductive process was needed. Lycopene which belongs to carotenoid group has a dark yellow pigment. This study aimed to determine the effect of adding lycopene on the semen quality of Saanen goats. The research design used 4 treatments, P0 (egg yolk tris diluent) as control), P1 (egg yolk tris diluent + 2% lycopene), P2 (egg yolk tris diluent + 4% lycopene), and P3 (yolk tris diluent). egg + 6% lycopene). The quality of semen was observed after 24 hours of storage at 5℃. Macroscopic data were analyzed descreptively and the microscopic one was analyzed using a completely randomized design (CRD). The results showed that the addition of lycopene in egg yolk tris diluent had a significant (P<0.05) effect on the semen quality of Saanen goats. The results showed that the motility of spermatozoa in each treatment group (P0, P1, P2, and P3) was 60.1 ± 5.7%, 65.2 ± 4.3%, 62.4 ± 5.2%, and 60.6 ± 4.7%, respectively, viability were 65.3 ± 4.8, 73.5 ± 4.2, 70.4 ± 3.8, and 66.1 ± 5.1, respectively, abnormality were 17 ± 1.8%, 16 ± 1.8%, 18 ± 1.9%, and 16 ± 1.7%, respectively, and membrane integrity were 63 ± 3.3%, 70 ± 3.6 %, 67 ± 3.2%, and 62 ± 2.9%, respectively. Based on the results, it could be concluded that the lycopene addition improved the semen quality of Saanen goats compared to controls.
ESTROGEN HORMONE PROFILE AND ESTRUS RESPONSE OF THIN TAILED EWES SYNCHRONIZED WITH CONTROLLED INTERNAL DRUG RELEASE Seraphina Kumala; widya Asmarawati; Ismaya Ismaya; sigit bintara; Riyan Nugroho Aji; Diah Tri Widayati
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.647 KB) | DOI: 10.21157/j.ked.hewan.v15i3.18583

Abstract

This research was conducted to identify the estrogen hormone profile and estrus response in the thin tailed ewes synchronized with controlled internal drug release (CIDR) implant. This research was carried out by employing 8 thin tailed ewes, CIDR, and estrogen kit. The ewes were distributed into: 3 ewes as control group and 5 ewes as treated group (with 12 days-CIDR implantation). Ewes were raised in the Sidomukti farm group, Sleman Regency, Yogyakarta. The data obtained were estrogen hormone profile during estrus examined by using ELISA method and estrus response marked with the reddening and oedema of vulva, mucus vaginal discharge, behavioral changes, vaginal pH, and superficial cells population. The data were analyzed by applying independent sample T-test. The result did not show any significant difference (P0.05) in the normal range between the control and CIDR group in estrogen hormone profile, estrus response, and pH level, but showed significant differences with positive correlation (P0.05) in population of superficial cells. In conclusion, CIDR was an effective tool for estrus synchronization at the farmer group, and gave positive estrus response towards thin tailed ewes with estrogen hormone profile, pH level, and superficial cell percentage on the normal range.
Co-Authors Achadiah Rachmawati Ajat Sudrajat Aji, Riyan Nugroho Aji, Riyan Nugroho Akhmad Fathoni Ali Agus Ali Agus Ali Agus Andriyani Astuti Budi Prasetyo Widyobroto Diah Tri Widayati Diah Tri Widayati Diahanvika Tri Sarvinda Dwi Astuti Dyah Maharani Eka Rizky Vury Rahayu Endang Baliarti Fatimah Az Zahra Chairunissa Heru Sasongko I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria Ikhwan Firhamsah Ismaya (Ismaya) Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Ismaya Kumala, Seraphina Kustantinah Kustantinah Kustono (Kustono) Labib Abdillah, Labib Luis Tavares Moh Takdir Nurul Hidayat Prajaningrum Luluk Miftahul Umami Priyadi, Dwi Ahmad Raden Febrianto Chsristi Rian Rosartio Riyan Nugroho Aji Seraphina Kumala Sri Harimurti Tety Hartatik Tri Anggraeni Kusumastuti Widya Asmarawati Yanuar Achadri Yudi Adinata Yuni Suranindyah