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Abdul Hafid B
Departemen Ilmu Bedah Saraf, Fakultas Kedokteran, Universitas AIrlangga
Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Thymoquinone Modulates Local MMP-9, IL-10, and IgG in Sciatic Nerve Crush Injury Animal Model Valentinus Besin; Abdul Hafid Bajamal; I Ketut Sudiana; Mangestuti Agil; Jusak Nugraha; Mohammad Hasan Machfoed; Paulus Budiono Notopuro; Naesilla Naesilla
The Indonesian Biomedical Journal Vol 14, No 3 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i3.2011

Abstract

BACKGROUND: Interleukin (IL)-10 is involved in Wallerian degeneration after peripheral nerve crush injury. Oral thymoquinone was previously observed to decrease local immunoglobulin-G (IgG) in a crush-injured rat model. No study has evaluated the pathway of various thymoquinone dosages on local IgG and IL-10 in this injury.METHODS: This experimental study used 126 Rattus norvegicus Wistar rats that were divided into 18 groups: six groups received a placebo, the other six groups received thymoquinone at 100 mg/kg/day and the last six groups received thymoquinone at 250 mg/kg/day, respectively. Rats were sacrificed at 12, 18, 24, 5x24, 6x24, and 7x24 hours. Matrix metalloproteinase-9 (MMP-9), IL-10, and local IgG levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA). The nuclear factor KappaB (NF-κB) expressions on Schwann cells were examined by flow cytometry. Path analysis was performed using SmartPLS.RESULTS: The path analysis showed that 100mg/kg/day of thymoquinone significantly decreased NF-κB expression. However, NF-κB did not affect local MMP-9, and MMP-9 had no significant relationship with local IL-10 and IgG. Thymoquinone 250 mg/kg/day also significantly inhibited NF-kB expression, decreased local MMP-9, and, in turn, decreased local IL-10 and IgG.CONCLUSION:  Administration of oral thymoquinone 250 mg/kg/day decreases local IgG and IL-10 levels via suppressing NF-κB expression and MMP-9 levels.KEYWORDS: thymoquinone, crush injury, IgG, IL-10, MMP-9, NF-κB
Co-Authors Agung Purniawan Amaliya Rasyida Ari Prasetyo Asadullah Asadullah Bal'afif, Farhad Besin, Valentinus Budi Utomo Endra Wibisono Harmawan Endra Wibisono Harmawan Farizah Mohd Hairi Galih Indra Permana Gunna Hutomo Putra Heru Kustono Jusak Nugraha Jusak Nugraha Komang Sena Adistira Artha Krisna Tsaniadi Prihastomo Lutfi Agus Salim M Aris Widodo M Istiadjid ES Mangestuti Agil Mohammad Hasan Machfoed Muhammad Faris Muhammad Fikri Kurniawan Muhammad Reza Arifianto Naesilla Naesilla Notopuro, Paulus Budiono Nurul Istifadah Resi Prastikarunia Rizki Meizikri S.Pd. M Kes I Ketut Sudiana . Setia Utama Subagio, Eko Agus Tedy Apriawan Yunus Kuntawi Aji Zaky Bajamal