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Analysis of Genetic Profiles of Heavy Metal Phytoremediator Plants From Gold Mining Areas Sih Winarti; Liswara Neneng; Yohanes Edy Gunawan; Chaidir Adam
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (522.732 KB) | DOI: 10.24036/0202041108305-0-00

Abstract

The resistance and survival ability of phytoremediator plants in a polluted environment is thought to be a form of genetic adaptation. This research aimed: (1) to identify the dominant plant species in ex-gold mining area; (2) to analyze the genetic profile of phytoremediator plants from ex-gold mining area; and (3) to compare the genetic profile of heavy metal phytoremediator plants from ex-gold mining area with the same plant species from non-mining area. The samples of this study were Cyperus sp., Lycopodium sp., and Melastoma sp. The research procedures carried out include sample collection, DNA isolation, DNA amplification with PCR, and DNA visualization with Electrophoresis. The results show that the dominant plant species of ex-gold mining area are Cyperus sp., Lycopodium sp., and Melastoma sp. The genetic profile analysis of dominant plant species of ex-gold mining area show that no DNA bands appeared from the target gene as the result of amplification using specific primers of Metallothionein gene. The result of RAPD analysis using OPA-04 universal primers show that at 500-750 bp there are differences in DNA bands that appeared between the samples. DNA bands that appeared in the genetic profile of phytoremediator plants is thought to be the representation of the gene that responsible for heavy metals tolerance.
Introduksi Teknologi Pengolahan Kacang Koro Pedang dalam Rangka Inisiasi Sentra Olahannya di Desa Tahai Baru Kalimantan Tengah Sih Winarti; Wijantri Kusumadati; Hastin Ernawati Nur Chusnul Chotimah; Gusti Irya Ichriani
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 5 No 3 (2020): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (390.791 KB) | DOI: 10.33084/pengabdianmu.v5i3.1248

Abstract

Tahai Baru Village is one of the villages in Maliku Subdistrict, Pulang Pisau Regency, Central Kalimantan, which received funding for the implementation of the koro sword (cangkordang) cultivation activities through the Joint Family Business Program (KUBE) program (PKH). Training and assistance need to be done considering that the Tahai Baru village community does not yet have the knowledge and skills and do not yet know the technology. The purpose of this activity is to increase the knowledge and skills of the people of Tahai Baru Village, Maliku District, Pulang Pisau Regency, in seeing alternative uses of natural resources, especially cangkordang, and increasing community income through processing and packaging of processed cangkordang products. Some approaches are carried out through counseling, training, mentoring, exposure of activities, and evaluating the sustainability and success of the program. The results of the activity showed that the community of Tahai Baru village had knowledge and skills about processing cangkordang and marketing several processed cangkordang products. The implementation of the Mitra Desa Development Program is expected to be able to bring a change in the impact on improving the social welfare of KUBE PKH members and establishing a sustainable partnership between Tahai Baru Village and Palangka Raya University.
Kompos dan pupuk organik cair untuk pertumbuhan dan hasil cabai rawit (Capsicum frutescens) di tanah gambut Hariyadi Hariyadi; Sih Winarti; Basuki Basuki
Journal of Environment and Management Vol. 2 No. 1 (2021): Journal of Environment and Management
Publisher : Program Pascasarjana Universitas Palangka Raya dan (and) Ikatan Ahli Teknik Penyehatan dan Teknik Lingkungan Indonesia (IATPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37304/jem.v2i1.2660

Abstract

Penggunaan kompos dan pupuk organik cair untuk pertumbuhan dan hasil cabai rawit di tanah gambut bertujuan untuk menelaah respons tanaman cabai rawit yang diberi pupuk kompos dan pupuk organik cair Mikro Alam 99 Plus tersebut dengan variasi takaran serta menetapkan takaran pupuk kompos dan pupuk cair yang memberikan pertumbuhan dan hasil terbaik pada tanah gambut. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) Split-Plot terdiri dari 2 faktor. Faktor pertama adalah pemberian pupuk kompos dengan 3 yaitu 0, 10 dan 20 t ha-1, sedangkan faktor kedua adalah pemberian Mikro Alam 99 Plus dengan 5 taraf yaitu 0, 10.000, 20.000, 30.000 dan 40.000 L ha-1. Kombinasi interaksi antara kompos kotoran sapi dan pupuk organik cair Mikro Alam 99 Plus terhadap pertumbuhan dan hasil cabai tidak memberikan interaksi yang nyata pada semua pengamatan. Sedangkan pada faktor tunggal dari beberapa variabel menunjukan pengaruh nyata pada variabel pengamatan Kandungan Air Relatif Daun pada perlakuan pupuk cair (dosis 30.000 L.ha-1) dengan nilai 81,19, jumlah cabang pada perlakuan kompos (10 ton ha-1) dengan nilai 9,40 dan berat buah pada perlakuan kompos (20 ton ha-1) dengan nilai 14,38. Pada pemberian kompos kotoran sapi yang optimal pada dosis 10 ton ha-1 sedangkan pemberian Mikro Alam 99 Plus yang optimal pada dosis 30.000 L ha-1.
PERTUMBUHAN DAN HASIL TANAMAN KEDELAI (Glycine max (l) Merr) YANG DIBERI PUPUK KOTORAN KAMBING DAN RHIZOBIUM SP PADA TANAH GAMBUT Winarti Sih; Sundari Yayang; Asi Yanetri
AgriPeat Vol. 17 No. 02 (2016): JURNAL AGRIPEAT VOLUME 17 NOMOR 02, SEPTEMBER 2016
Publisher : Fakultas Pertanian Universitas Palangka Raya

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Abstract

This study aimed to examine the response of soybean plants to organic fertilizer goat manure and Rhizobium sp on peat soil, and determine the goat manure fertilizer and Rhizobium sp which gives the growth and yield of soybean best in peat soil. This experiment used a block randomized factorial design with two factors treatments and 3 replications. The first factor is the goat manure consists of three levels ie 0 , 10 and 20 t ha-1 and the second factor is the Rhizobium sp consists of five levels ie 0 , 4 , 8 , 12 and 16 g kg-1 seed.The results showed that the highest number of root nodules on soybean obtained by goat manure dose of 20 t ha-1 and 12 g kg-1 seed Rhizobium sp. Fertilization goat manure at a dose of 10 t ha-1 on peat, soybean plants tend to grow taller, wider leaves, root nodule weight effectively, the weight of pods, seed weight per plant and 1000 grain weight was significantly higher and the real number of pods per plant more than in the controls. GivingRhizobiumspon peat soilwitha dose of12gkg-1seed, is capable of significantly increased plant height, leaf area, number of root nodules effectively, the weightof nodulesperplant, weightof pods perplant, seed weight perplant and weight of 1000 seeds of soybean compared with nogiven Rhizobiumsp (control)
PENGARUH KOMPOS LIMBAH BAGLOG JAMUR TIRAM DAN PUPUK HAYATI TERHADAP PERTUMBUHAN DAN HASIL TANAMAN CABAI KERITING (Capsicum annum L.) PADA TANAH GAMBUT PEDALAMAN Sinaga T. M. T.; Winarti Sih; Asie K. V.
AgriPeat Vol. 16 No. 02 (2015): JURNAL AGRIPEAT VOLUME 16 NOMOR 02, SEPTEMBER 2015
Publisher : Fakultas Pertanian Universitas Palangka Raya

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Abstract

The objectives of this study namely analyze of waste baglog of effect oyster mushrooms compost to growth and yield curly chilli on Peat Land and to find out the doses of waste baglog of oyster mushrooms compost which give the best curly chilli growth and yield. The experimental design used was a completely randomized design factorial (CRD), which consists of two factors treatments. The first factor was giving the waste baglog of oyster mushrooms compost which consists of four levels, namely: control, 100, 200 and 300 gpolybag-1. The second factor was giving the biofertilizer which consists of three levels, namely: control, 40 and 60 gpolybag-1.The results showed that the interaction waste compost baglog oyster mushrooms and biofertilizer only be obtained at the age of two weeks after planting the plant height chilli curls and composting waste baglog oyster mushrooms very significant effect on plant height, stem diameter, days to flowering plants, productive branches, the amount of fruit and fresh weight per plant. Composting 100 g polybag-1 is the best dosage. Biofertilizer application significantly higher only in the high growth of curly chilli plants
https://doi.org/10.36873/ PERTUMBUHAN DAN HASIL BAWANG DAYAK (Eleutherine americana (L) Merr) YANG DIBERI EMPAT JENIS KOTORAN TERNAK PADA TANAH GAMBUT DAN SPODOSOL: The Growth And Yield Dayak Onion (Eleutherine americana (L) Merr) Fertilized ByFour Kinds Of Manure On Peat And Spodosol Sih Winarti; Zafrullah Damanik; Rentihen Penyang
AgriPeat Vol. 22 No. 2 (2021): JURNAL AGRIPEAT VOL. 22 NO. 2, SEPTEMBER 2021
Publisher : Fakultas Pertanian Universitas Palangka Raya

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Abstract

The purpose of this study was to determine the growth and yield of dayak onion fertilized by four kind of manure on peat and spodosol. This experiment used a factorial completely randomized design, consisting of two treatment factors with four replications. The treatments were the types of fertilizer (poultry, swallow, cow, goat) and soil types (spodosol, peat). The results showed that growth of plant height, fresh weight, number of tubers, tuber diameter of dayak onion plants that were given chicken manure, swallow manure, cow manure, and goat manure were relatively the same, only the number of leaves that were given cow manure was significantly higher. Onion plants dayak planted on spodosol soil grew better, i.e. taller plants, more number of leaves and number of tubers, larger tuber diameter and fresh weight than those planted on peat soil
PENGARUH PUPUK ORGANIK CAIR DAN PUPUK MAJEMUK NPK TERHADAP PERTUMBUHAN SETEK BATANG CINCAU HIJAU: Effect of Liquid Organic Fertilizer and NPK Compound Fertilizer On The Growth of Green Grass Cincau (Premna oblongifolia Merr) Stem Cuttings in Peat Soil Agung Prasetyo; Sih Winarti; Siti Zubaidah; Yustinus Sulistiyanto; Hastin Ernawati Nur Chusnul Chotimah
AgriPeat Vol. 23 No. 2 (2022): JURNAL AGRIPEAT
Publisher : Fakultas Pertanian Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36873/agp.v23i2.5960

Abstract

This study was to determine the effect of NPK and liquid organic fertilizers and the interaction on the growth of green grass jelly stem cuttings on peat soil. The research was carried out in September - December 2020, located in the plastic house of the Experimental Garden Installation Department of Agricultural Cultivation, Faculty of Agriculture, University of Palangka Raya. This experiment used a factorial completely randomized design (CRD) consisting of 2 (two) treatment factors with 3 (three) replications. The first factor was the concentration of POC administration consisting of 4 (four) levels (0, 3, 6 and 9 mL of POC L-1 water) and the second factor is the dose of NPK 15: 15: 15 fertilizer consisting of 4 (four) levels ( 0, 1, 2 and 3 g NPK polybag-1). The results showed that there was an interaction between the administration of 3 mL POC L-1 water and 3 g NPK polybag-1 on shoot length (77.57 cm) and the treatment of 9 mL POC L-1 water and 3 g NPK polybag-1 on weight. fresh leaves (53.83 g). A single factor of 3 g NPK polybag-1 fertilizer was able to give a significant effect on the number of shoots (4.58 pieces), shoot length (75.31 cm), number of leaves (38.33 strands), leaf area (3032.77 cm2), heavy fresh leaves (54.86 g) root length (30.99 cm) and root volume (7.00 cm-3). The provision of 9 mL of POC L-1 water gave a significant effect on the number of roots in the treatment (10.58 fruit) and root weight in the treatment of 9 mL of POC L-1 water (2.39 g)
SIFAT KIMIA TANAH, PERTUMBUHAN DAN HASIL TANAMAN MELON (Cucumis melo L.) YANG DIBERI KASCING DAN ZAT PENGATUR TUMBUH PADA SPODOSOL: The Chemical Properties, growth an yield of Meloon (Cucumis melo L.) Which given vermicompost and growth Regulatory Substances on Spodosol Sih Winarti; Basuki Basuki; Sri Endang Agustina Rahayuningsih; Panjaitan A. R; Sinarmata T. J.
AgriPeat Vol. 23 No. 2 (2022): JURNAL AGRIPEAT
Publisher : Fakultas Pertanian Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36873/agp.v23i2.5971

Abstract

This study aims to determine the effect of vermicompost fertilizer on changes in the chemical properties of spodosol as a growing medium for melons and to determine the growth and yield of melons treated with gibberellins as growth regulators. The study used a Factorial Completely Randomized Design. The first factor is the dose of vermicompost 0, 60, 90, 120 and 150 g vermicompost per polybag equivalent to 0, 10, 15, 20 and 25 t. vermicompost ha-1. The second factor is the provision of plant growth regulators Agrogibb 40 SL given in the generative phase consisting of 2 two levels, namely: 0 and 0.5 mL L-1 with 4 replications. The results showed that the application of vermicompost fertilizer on spodosols was able to increase soil pH and available P, but organic C and total soil N tended to decrease, plants were able to grow significantly better. There was no interaction between the application of vermicompost fertilizer and plant growth regulators giberelin on the yield of melons. Kascing fertilizer with a dose of 90 g plant-1, the fruit flesh was thicker and the highest tight weight was obtained at a dose of 120 g plant-1. The application of gibberellin growth regulator with a concentration of 0.5 mL L-1 was able to significantly increase fruit weight, fruit diameter, fruit thickness, wet and dry weight of melon plants