Articles
<![CDATA[Tingkat Pengetahuan terhadap Donor Darah pada Masyarakat Pendonor ]]>
<![CDATA[Afrianti, Dina]]>;
<![CDATA[Susilawati, Elis]]>;
<![CDATA[Sofyanita, Eko Naning]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 4 No. 1 (2022): May 2022 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v4i1.8445
<![CDATA[Blood donation is an activity to donate blood for transfusion purposes. The number of voluntary blood donors in Indonesia is around 20% of the total production of blood bags per year, the rest is met from replacement donors. People are generally reluctant to donate blood because of fear of pain when blood is drawn using a syringe, fear of running out of blood, resulting in anemia, contracting the disease through blood donors, feeling unwell to donate blood and feeling that it is enough to only donate blood once. This is a factor that causes the limited number of donors due to limited knowledge. Research Objectives: To see an overview of the level of knowledge about blood donors in the community of donors at Pekalongan City. Methods: The type of research used is descriptive quantitative with a cross sectional approach with a questionnaire to determine the level of knowledge of the donor community. Results: Most of the blood donors at the Pekalongan City are Male 73 respondents (73%), the highest percentage aged 17-30 years as many as 54 respondents (54%), high school education, namely as many as 49 respondents (49%). Most of the donor knowledge level is said to be good, namely 88 respondents (88%). Conclusion: Most of the donor community at the Pekalongan City have a good level of knowledge about blood donation.]]>
<![CDATA[Kejadian Toksoplasmosis pada Wanita Hamil Pemelihara Kucing dan Wanita Hamil Bukan Pemelihara Kucing di Puskesmas Tlogosari Wetan Kota Semarang ]]>
<![CDATA[Afrianti, Dina]]>;
<![CDATA[Swastya Putri, Adita Puspitasari]]>;
<![CDATA[Wahyudi, Wahyudi]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 5 No. 1 (2023): May 2023 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v5i1.9396
<![CDATA[Toxoplasma gondii (T. gondii) is a widespread zoonotic pathogen worldwide. This parasite grows inside the body of a cat as definitive host. Cats play an important role in the epidemiology of T. gondii because they are the only animal capable to shed oocysts which can withstand outside environment. Nowadays, transmission of T. gondii infection from cat to human increases in particular areas. This study aims to identify the incidence of toxoplasmosis in pregnant women Raise Cats and Not Raise Cats in Tlogosari Wetan Health Center Semarang City. This study was an descriptive observational research with case control design in two populations. Subjects are chosen based on certain criteria, This study was done to 25 respondents as pregnant women raise cats and 25 respondents who weren’t. The results obtained from serological analysis on 50 blood serum samples consisting of 25 blood serum samples of pregnant women raise cats with positive IgG antibody as many as 6 serum samples (24%) and 25 blood serum samples of pregnant women who are not raise cats obtained positive IgG antibody in 2 samples serum (8%). The total percentage of toxoplasmosis was 16% from 50 serum samples. It is necessary to carry out a large-scale serological survey to determine the prevalence of toxoplasmosis in pregnant women in Semarang City and necessary monitoring and treatment of pregnant women infected with Toxoplasma gondii.]]>
<![CDATA[The Relationship of Zink and Iron Levels with Blood Pressure in Pulmonary Tuberculosis Patient ]]>
<![CDATA[Kurniasih, Dewi]]>;
<![CDATA[Muslina, Muslina]]>;
<![CDATA[Latifah, Aminahtun]]>;
<![CDATA[Afrianti, Dina]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 5 No. 2 (2023): November 2023 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v5i2.10415
<![CDATA[Background: Mycobacterium tuberculosis infection in Tuberculosis (TBC) causes narrowing of the pulmonary blood vessels and triggers pulmonary hypertension which is associated with iron deficiency and anemia. The mineral zinc (Zn) is known to play an important role in destroying the bacteria that cause TBC. In addition, iron (Fe) deficiency in TBC patients can cause relapse and even treatment failure. This study aims to examine the relationship between serum zinc and iron levels and blood pressure in pulmonary TBC patients. Method: A descriptive analytical observational study with a cross sectional design was conducted on 51 TBC patients undergoing treatment at the Simpang IV Sipin Community Health Center, Jambi City. Using a purposive sampling technique, respondents who met the research inclusion and exclusion criteria were examined for zinc and Fe levels. The examination was carried out at the Jambi Province Regional Health Laboratory, the examination result data was then analyzed bivariately using the chi square test. Results: The results of statistical analysis between blood pressure and Zn levels obtained a significant value with P value = 0.877 and a significant value for Fe levels obtained P value = 0.841 (> 0.05). Conclusion: There is no significant relationship between Zn and Fe levels and blood pressure in pulmonary TBC patients. Based on research, it is known that iron deficiency and anemia are not directly related to pulmonary hypertension which can be experienced by TBC patients.]]>
<![CDATA[Uji Kualitas Telur Cacing STH Menggunakan Pewarna Alami Perasan Daun Jati (Tectona grandis) dengan Konsentrasi 40%, 60%, 80%, 100% sebagai Pewarna Alternatif Metode Sedimentasi ]]>
<![CDATA[Anggraini, Maysita Ari]]>;
<![CDATA[Afrianti, Dina]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 6 No. 2 (2024): November 2024 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v6i2.11625
<![CDATA[Helminthiasis is a parasitic infection in the form of worm larvae or eggs that develop in the human body. Diagnosing helminthiasis with fecal examination uses 2% eosin dye to clarify and emphasize the shape of worm eggs. Eosin 2% is a class-3 IARC carcinogenic reagent, so a natural reagent that does not damage the environment is needed. Reagents from natural materials that can be utilized are teak leaves containing anthocyanin compounds that can clarify and emphasize the shape of worm eggs. The purpose of this study was to determine the quality of sth (soil transmitted helminth) worm eggs using natural dye teak leaf juice (tectona grandis) with a concentration of 40%, 60%, 80%, 100% as an alternative dye sedimentation method. The design of this study is quantitative with experimental design with posttest with control group design with Kruskal-Walis test. The results of this study obtained the concentration with the highest average value, namely 40% concentration of 8.7. The higher the concentration, the less clear the morphology of the egg because of the influence of dirt / residue from the juice. The results of the Kruskal-Walis test sig value 0.054, it can be concluded that there is no difference between teak leaf juice with a concentration of 40%, 60%, 80%, and 100% with 2% eosin dye as a control. Based on the mean rank value of the Kruskal-Walis test, it can be seen that the best teak leaf juice concentration is 40% concentration with a value of 34.50. This research can be used as a reference for the utilization of teak leaves as a natural dye to replace 2% eosin for STH worm eggs.]]>
<![CDATA[Mentoring and Empowerment Diploma Three Student in Blood Bank Technology, Health Polytechnic, Ministry of Health, Semarang, Through Training in Making Soy Milk as an Effort to Prevent Anemia ]]>
<![CDATA[Afrianti, Dina]]>;
<![CDATA[Hartono, Rodhi]]>;
<![CDATA[Setyowatiningsih, Lilik]]>;
<![CDATA[Sofyanita, Eko Naning]]>;
<![CDATA[Wahyudi]]>
<![CDATA[ Jurnal Ilmiah Pengabdian Masyarakat Bidang Kesehatan (Abdigermas) Vol. 2 No. 1 (2024): Jurnal Ilmiah Pengabdian Kepada Masyarakat Bidang Kesehatan (Abdigermas) ]]>
Publisher : <![CDATA[CV Media Inti Teknologi ]]>
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DOI: 10.58723/abdigermas.v2i1.169
<![CDATA[Mentoring participants know about anemia and its symptoms. Participants know the equipment and materials needed to make soy milk and know the steps on how to make soy milk and participants are able to give a demonstration of making soy milk to the residents of the target village. Residents of the assisted villages know how to make soy milk. Community service activities with the Entrepreneurship Development Program (PPK) scheme with the theme "Assistance in the Empowerment of Diploma Three Technology Students at the PolkesMar Blood Bank through training in making soy milk (Soymilk) as an effort to prevent anemia" was carried out on Friday 28 July 2023 with a target of 10 people participating. consisting of 8 active students and 2 alumni. The output is in the form of videos and pamphlets on making soy milk that have received IPR certificates as well as the expertise of training participants resulting from a series of training activities on making soy milk (Soymilk) targeting students and alumni. This has also been implemented in community service activities for the target village residents in Pundan Hamlet, Subdistrict. Kebondowo Banyubiru, Semarang district, which will be held on Sunday 15 October 2023. The successful achievement of this activity was that all participants in the training on making soy milk were able to give demonstrations on making soy milk to the residents of the target villages. Residents of the assisted villages were given education about anemia and solutions to preventing anemia, the committee also showed videos of making soy milk, distributed pamphlets and gifts to residents of the assisted villages.]]>
<![CDATA[Efektivitas Masa Penyimpanan Filtrat Daun Jati (Tectona grandis) terhadap Kualitas Pewarna Preparat Telur STH (Soil Transmitted Helminth) ]]>
<![CDATA[Rahmadhani, Afiffah Gita]]>;
<![CDATA[Afrianti, Dina]]>;
<![CDATA[Priyatno, Djoko]]>
<![CDATA[ Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025 ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Semarang ]]>
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DOI: 10.31983/jlm.v7i2.13018
<![CDATA[Soil-Transmitted Helminth (STH) infections such as Ascaris lumbricoides remain a public health concern in Indonesia, necessitating microscopic fecal examinations using high-quality stains. Eosin is commonly used but poses environmental and health risks. Anthocyanins found in teak leaf (Tectona grandis) filtrate offer a safer and more eco-friendly alternative. One factor affecting anthocyanin color stability is storage duration. This study aimed to evaluate the effectiveness of teak leaf filtrate stored for 0, 7, 14, and 21 days on the staining quality of STH egg preparations. This quasi-experimental study used a Posttest-Only Nonequivalent Control Group Design. The samples consisted of 24 Ascaris lumbricoides egg preparations divided into four treatment groups with six replications each. Staining quality was assessed by three validators based on contrast under the microscope, color absorption, and morphological clarity. Data were analyzed using the Kruskal-Wallis test to determine differences in staining quality across storage durations. All preparations showed good staining quality, and no significant differences were found between the storage groups (p-value = 0.383; > 0.05). These results indicate that teak leaf filtrate remains effective for staining STH egg preparations for up to 21 days of storage, with the best results observed at 7 days after preparation. Further research is recommended to explore this topic more deeply, with attention to laboratory procedures and technical skills.]]>
<![CDATA[Pemberdayaan Masyarakat tentang Tuberkulosis serta Pelatihan Etika Batuk di Kelurahan Sendang Mulyo: Community Empowerment about Tuberculosis and Cough Ethics Training in Sendang Mulyo ]]>
<![CDATA[Afriansya, Roni]]>;
<![CDATA[Budiharjo, Teguh]]>;
<![CDATA[Afrianti, Dina]]>;
<![CDATA[Widodo, Widodo]]>;
<![CDATA[Kuncara, Rachmad Bayu]]>
<![CDATA[ PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 8 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat ]]>
Publisher : <![CDATA[Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya ]]>
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DOI: 10.33084/pengabdianmu.v9i8.7021
<![CDATA[Tuberculosis control requires synergy between the government and the community. The success of TB control in an area is inseparable from the role of the community, which can be pursued by empowering the community. Community empowerment in TB control is to foster awareness, ability and willingness in breaking the chain of TB transmission. This activity aims to increase the knowledge and ability of the community in breaking the chain of TB transmission. The method of activity is carried out by providing counseling and training directly to the community. The results of the activity showed an increase in knowledge and ability of the community in understanding about TB. Direct counseling on TB and direct training on how to cough properly have a good and effective impact in increasing the knowledge and abilities of the community. It is recommended that empowerment activities by providing direct material about TB and direct training on how to cough properly be carried out more intensively and also involve health cadres.]]>
<![CDATA[Screening for the Detection of IgG anti-Toxoplasma gondii Antibodies in Female Donors at Semarang Regency ]]>
<![CDATA[Afrianti, Dina]]>;
<![CDATA[Putri, Adita Puspitasari Swastya]]>;
<![CDATA[Wikandari, Ririh Jatmi]]>;
<![CDATA[Wahyudi, Wahyudi]]>
<![CDATA[ Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 4 (2024): February ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Palu ]]>
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DOI: 10.33860/jik.v17i4.2862
<![CDATA[Toxoplasmosis is a widespread zoonotic disease caused by Toxoplasma gondii (T.gondii). Blood and blood products are possible routes of transmission of T. gondii, especially in patients who have undergone multiple transfusions. The aim of this study is to determine the seroprevalence of toxoplasmosis in female donors using blood serum which is analyzed serologically to detect ImmunoglobulinG (IgG) using the Enzyme-Linked Fluorescent Assay (ELFA) method, as well as the relationship between risk factors for toxoplasmosis, such as keeping cats, receiving blood transfusions, eating undercooked meat and eating raw vegetables. This research is an analytic observational study using a cross-sectional study design. The sample taken is female donor candidates who are willing to participate during the study period, from March to September 2023, and have met the inclusion criteria to become research subjects by explaining the purpose and procedure of the study and the consent letter signed by each research subject before blood samples were taken and filling out the research questionnaire. 177 blood serum samples of female donors were collected from blood donor candidates using consecutive sampling method and a questionnaire to obtain risk factor data that influence the incidence of toxoplasmosis. The results showed that the seroprevalence of toxoplasmosis in the blood of female donor at Indonesian Red Cross Blood Donor Unit (UDD PMI) Semarang Regency, Central Java Province was 26.6%. In this study the risk factors that influence the incidence of toxoplasmosis are consuming raw vegetables with 5.835 times more risk than women who do not consume raw vegetables, while other factors such as keeping cats, receiving blood transfusions, and eating undercooked meat, have no significant effect. The seroprevalence of toxoplasmosis in female donors at UDD PMI of Semarang Regency is quite high due to the habit of eating raw vegetables or fresh vegetables with a very high transmission potential.]]>
<![CDATA[Sungkai Leaf Extract (Peronema canescens Jack) Reduces MDA Levels and Increases IL-10 Levels in MSG-Induced Wistar Rats ]]>
<![CDATA[Wahyudi, Wahyudi]]>;
<![CDATA[Chodijah, Chodijah]]>;
<![CDATA[Sumarawati, Titiek]]>;
<![CDATA[Afrianti, Dina]]>
<![CDATA[ Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 3 (2023): November ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Palu ]]>
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DOI: 10.33860/jik.v17i3.3015
<![CDATA[The addition of Monosodium Glutamate (MSG) to ready-to-eat food can change eating habits, coupled with a lack of activity which can have an impact on health. MSG as a trigger for increased reactive oxygen species (ROS), causing systemic damage, the impact of excessive MSG consumption can be reduced by modifying the diet to contain high sources of antioxidants. Sungkai leaf extract can be an alternative as a natural antioxidant. Sungkai leaf extract antioxidants are expected to neutralize ROS thereby repairing cell damage. Objective: This study aims to determine how giving sungkai leaf extract affects MDA and IL-10 levels in MSG-induced mice. Method: Experimental research with a Randomized Post test only control group design. The total sample was 24 male Wistar rats divided into 4 groups. KN healthy mice, K(-) were given 1g MSG/rat, P1 was given 1g MSG/rat and 28mg sungkai leaf extract/rat, P2 was given 1g MSG/rat and 56mg sungkai leaf extract/rat. Results: The average results showed a decrease in MDA levels and an increase in IL-10 levels after treatment for 21 days, the One way Anova test followed by Post hoc LSD showed that each treatment group was significantly different from the control group where the MDA level in group P2 was 0, 07mg/ml ± 0.01 experienced a significant decrease compared to the KN group 0.07mg/ml ± 0.01 while IL-10 levels experienced a significant increase in the P1 group 130.10pg/ml ± 13.29 when compared to the KN group 60.43pg/ml ±17.40. Conclusion: Sungkai leaf extract (Penonema Canescens Jack) was able to reduce MDA levels at a dose of 56 mg/mouse in mice injected with 1gr MSG/rat and experienced a significant increase in IL-10 levels with a dose of 28 mg sungkai leaf extract/rat injected with 1gr MSG/rat.]]>
<![CDATA[Evaluation of Toxoplasmosis in Female College Students ]]>
<![CDATA[Wikandari, Ririh Jatmi]]>;
<![CDATA[Afriansya, Roni]]>;
<![CDATA[Kuncara, Rachmad Bayu]]>;
<![CDATA[Afrianti, Dina]]>;
<![CDATA[Setyowatiningsih, Lilik]]>
<![CDATA[ Poltekita : Jurnal Ilmu Kesehatan Vol. 17 No. 4 (2024): February ]]>
Publisher : <![CDATA[Poltekkes Kemenkes Palu ]]>
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DOI: 10.33860/jik.v17i4.3209
<![CDATA[Female students are a group of women of childbearing age who can become pregnant and have risk factors for toxoplasmosis which can cause pregnancy disorders, birth defects, and death. Seroepidemiological studies of toxoplasmosis in women are essential to determine whether infection occurs in the early stages of fertilization or earlier. The research aimed to determine the incidence of toxoplasmosis in female college students. This research was carried out in June 2023 and has received ethical recommendations from KEPK Poltekkes Kemenkes Semarang. The type of research used was analytical descriptive. Sampling used purposive sampling. The inclusion criteria were 87 students who were willing to have their blood taken. Blood is taken using the venipuncture method, then the whole blood is separated from the plasma. Plasma samples were collected and analyzed for the presence of anti-Toxoplasma gondii using the ELISA method. Then the data was analyzed using SPSS software, univariate analysis to determine the frequency distribution and percentage of each variable. The results showed that 31 students were positive for IgG anti-Toxoplasma gondii. Of these, the majority aged 20 years (n = 20, 16.1%) were positive for anti-Toxoplasma gondii IgG. Toxoplasmosis infection of 9.2% occurred in female college students who owned cats, 3.4% occurred in female college students who consumed undercooked meat, and 29.8% of female college students who consumed raw vegetables. Female college students have risk factors for Toxoplasma gondii infection so it is important to carry out screening so that Toxoplasma gondii can be detected and treated immediately.]]>
