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SCREENING BAKTERI SELULOLITIK DAN AMILOLITIK PADA RUMEN SAPI SEBAGAI KANDIDAT PROBIOTIK PADA BUDIDAYA IKAN SECARA IN VITRO Anis Zubaidah; Dony Prasetyo; Hany Handajani; Sulis Puji Rohmah; Dyah Ayu Puspita
Jurnal Riset Akuakultur Vol 14, No 4 (2019): (Desember, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.303 KB) | DOI: 10.15578/jra.14.4.2019.261-271

Abstract

Bakteri selulolitik dan amilolitik mampu mengubah selulosa dan amilum menjadi glukosa serta mampu menghasilkan enzim selulase dan amilase. Bakteri selulolitik dan amilolitik diisolasi dari rumen sapi. Tujuan dari penelitian ini adalah untuk mendapatkan bakteri selulolitik dan amilolitik dari rumen sapi yang memiliki nilai indeks selulolitik dan amilolitik yang tinggi, serta mampu memenuhi syarat untuk dijadikan sebagai probiotik. Screening bakteri pada rumen sapi menghasilkan enam isolat yaitu AR, BR, CR, DR, ER, dan FR. Uji aktivitas selulolitik dilakukan pada substrat carboxy methyl cellulose (CMC) dan amilolitik pada amilum, uji ketahanan terhadap kondisi asam (pH 3), pengamatan pertumbuhan bakteri selama 30 jam, uji antagonistik terhadap bakteri patogen Aeromonas hydrophila, uji penempelan bakteri dan uji patogenisitas bakteri pada ikan. Hasil penelitian menunjukkan bahwa aktivitas selulolitik terbesar pada isolat AR (2,67 ± 0,35 cm) dan aktivitas amilolitik terbesar pada isolat AR (4 ± 0,60 cm). Hanya empat isolat (AR, BR, ER, dan FR) yang dilakukan uji lanjut. Keempat isolat mampu bertahan dalam kondisi asam pH 3 selama delapan jam dengan nilai OD terbesar pada isolat ER (1.137). Uji antagonistik menunjukkan bahwa isolat AR, ER, dan FR mampu menghambat pertumbuhan bakteri Aeromonas hydrophila. Isolat FR memiliki nilai antagonistik terbesar yang ditandai dengan terbentuknya zona hambat 17 mm. Uji penempelan bakteri untuk membuktikan bahwa isolat mampu menempel pada usus ikan. Uji patogenesitas pada isolat yang didapatkan guna membuktikan bahwa isolat tidak bersifat patogen terhadap inang. Berdasarkan hasil penelitian dapat disimpulkan bahwa isolat yang didapatkan dari rumen sapi termasuk bakteri selulolitik dan amilolitik yang telah memenuhi syarat sebagai kandidat probiotik pada ikan.Cellulolytic and amylolytic bacteria can transform cellulose and starch into glucose and produce the cellulase and amylase enzymes. These types of bacteria can be found in and isolated from cow’s rumen. Thus, the purpose of this study was to obtain potential cellulolytic and amylolytic bacteria from cow’s rumen with a high cellulolytic and amylolytic index value and can be qualified as probiotics. The screening of bacteria in the cow’s rumen produces six isolates i.e. AR, BR, CR, DR, ER, and FR. The parameters observed were: the cellulolytic activity in carboxymethyl cellulose (CMC) substrate, amylolytic activity on starch substrates, resistance test to acidic conditions (pH 3), bacterial growth for 30 hours, antagonistic to pathogenicity test to bacteria Aeromonas hydrophila, bacterial attachment test, and bacterial pathogenicity test in fish. The results showed that the largest cellulolytic and amylolytic activities were observed in AR isolates with a value of 2.67 ± 0.35 cm) and 4 ± 0.60 cm, respectively. Only four isolates (AR, BR, ER, and FR) were used in further tests. The four isolates were able to survive in the acidic conditions of pH 3 for 8 hours with the largest (OD) value was achieved by ER isolates (1,137). The growth of each isolate was different. The antagonistic test showed that the three isolates could inhibit the growth of A. hydrophila. FR isolates had the greatest antagonistic values characterized by the formation of an inhibition zone of 17 mm. Bacteria attachment test proved that the isolates were able to stick in the fish gut. The pathogenicity tests also proved that the isolates were not pathogenic to the host. 
PENERAPAN MANAJEMEN BUDIDAYA IKAN NILA YANG BAIK DIKELOMPOK PEMBUDIDAYA IKAN GEMARI JAYA KABUPATEN MALANG Hany Handajani; Ganjar Adhywirawan Sutarjo
Jurnal Abdi Insani Vol 9 No 2 (2022): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v9i2.534

Abstract

The group of fish cultivators who continue to strive to develop intensive freshwater fish farming activities in the Jedong Village, Malang Regency, is the Gemari Jaya fish cultivator group. This fish farming business is relatively new, which was formed in 2019 so it requires assistance from various parties including universities. In running their business, program partners face several problems, namely the lack of proper management of fish farming so that costs and production capacity are not measurable. The purpose of this program is to improve the skills and knowledge of partners regarding the implementation of good Tilapia aquaculture management, the application of water quality management, and the application of fish health management. The method used in this activity is a method of training and mentoring related to the importance of improving good management of tilapia aquaculture, water quality management, and fish health management. The results of the activities obtained are that partners are able to apply management of Tilapia fish farming intensively guided by good fish farming practices (CBIB), Application of water quality management techniques through periodic water quality monitoring, and application of herbal plant-based fish health management at partner locations so that production capacity previously 40% increased to 60%, the target production time from the previous 6 months can be achieved within 5 months. The conclusion of this activity is that the production capacity and time of tilapia cultivation can be measured properly, so that the partner's economy increases.
Peran Gender dalam Keluarga Nelayan Tradisional dan Implikasinya pada Model Pemberdayaan Perempuan di Kawasan Pesisir Malang Selatan Hany Handajani; Rahayu Relawati; Eko Handayanto
Jurnal Perempuan dan Anak Vol. 1 No. 1 (2015): Januari
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.231 KB) | DOI: 10.22219/jpa.v1i1.2745

Abstract

Peran Gender dalam Keluarga Nelayan Tradisional dan Implikasinya pada Model Pemberdayaan Perempuan di Kawasan Pesisir Malang Selatan1) Hany Handajani, 2) Rahayu Relawati, 3) Eko Handayanto1Jurusan Perikanan Universitas Muhammadiyah Malang, Jln. Raya Tlogomas no. 246, Malang 65144; 2Jurusan Agribisnis Universitas Muhammadiyah Malang, Jln. Raya Tlogomas no. 246, Malang 65144; 3Jurusan Manajemen Universitas Muhammadiyah Malang, Jln. Raya Tlogomas no. 246, Malang 65144; *Email : hannyhandajani.umm@gmail.comAbstract :Nelayan tradisional masih mempertahankan cara penangkapan ikan, bekerja tanpa inovasi teknologi, tanpa dukungan modal kuat, tanpa kelembagaan usaha yang mapan. Tujuan jangka panjang penelitian adalah terbinanya nelayan tradisional berdasarkan perspektif gender. Dalam jangka pendek perlu dirumuskan metode pembinaan perempuan khususnya dan keluarga nelayan tradisional pada umumnya setelah melalui serangkaian analisis gender. Penelitian dilakukan di pesisir Sendangbiru, Malang Selatan. Sempel keluarga nelayan tradisional ditentukan secara random. Data diambil melalui wawancara terstruktur, wawancara mendalam dan observasi partisipatif. Data dianalisis secara deskriptif dan dengan analisis gender metode Harvard. Keywords :nelayan tradisional, aktivitas melaut, aktivitas pasca tangkap ikan, pembinaan
MEAT BONE MEAL (MBM) AND POLTRY BY-PRODUCT (PBM) TEST ON GRANULE TOWARDS THE GROWTH AND SURVIVAL RATE OF EEL (Anguilla Bicolor) Stadia ELVER Triana Indri Astuti; Hany Handajani; Riza Rahman Hakim
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 1 No. 1 (2018): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v1i1.5855

Abstract

This research was conducted at Hatchery Fishery Laboratory in University of Muhammadiyah Malang on February 15 - March 17, 2018. The purpose of this research was to identify the meat bone meal (MBM) and poltry by-product (PBM) test on granule and the best treatment to the growth and survival rate of Eel (Anguilla bicolor) stadia elver. The method used was experiemental by Completely Randomized Design (RAL) with 3 different treatments on granule composition, P1 (Commercial Granule), P2 (70% Commercial Granule  and 30% MBM), P3 (70% Commercial Granule and 30% PBM), and each treatment done repeatedly three times. Furthermore, the data were analyzed by ANAVA and continued with BNT test. The results of this study indicated that the meat bone meal (MBM) and poltry by-product (PBM) test on granule had a significant effect on the absolute growth rate, a very significant effect on DGR, and not significantly different on Eel. The absolute best growth rate was on P2 2.24 ± 0.71. The best DGR was on P1 (5.55 ± 0.33). Based on the research results, it was concluded that feeding the granule based on meat bone meal increased the growth and survival rate, and also could be the substitution granule for Eel ( Anguilla bicolor). Keywords: Anguilla bicolor, MBM, PBM, Growth
Effect of different stocking densities on growth and survival of Seabass Fish (Lates calcarifer) nursery phase in low salinity Mochammad Noval; Hany Handajani; Hariyadi Hariyadi
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 2 No. 2 (2019): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v2i2.9595

Abstract

Increased production of Seabass fish (Lates calcarifer) influences the stocking density used. The purpose of this study was to determine the growth and survival of Seabass (Lates calcarifer) at different densities of low salinity stocking. The study was conductedin May-June 2019 at the Laboratory of Fisheries, University of Muhammadiyah Malang. The material used in the study of Seabass fish (Lates calcarifer) as many as 550 fish with a size of 7 cm and fish feed in the form of B2 Stella. The method used was experimental with a completely randomized design (CRD) with 4 treatments and 3 replications, namely 1 fish/2 L as control (A); 2 fish/2 L (B); 3 fish/2 L (C); 4 fish/2 L (D). Data analysis used anova(P <0.05) and tested further with the Least Significant Difference Test of 5%. The results showed that treatment B (51 ± 2 fish/2 L) produced a specific growth rate of length and weight, survival rate, efficiency of feed utilization, and feed conversion ratio which was better than treatment A (67 ± 1 fish/2L (control)), C (44 ± 3 fish/2 L and D (38 ± 4 fish/2 L).
Trend analysis of catching fish Decapterus sp. in the Muncar Coastal Fisheries Port, Banyuwangi, Indonesia Devi Purwinda; Hany Handajani; Anis Zubaidah
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 3 No. 2 (2020): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v3i2.12939

Abstract

Small pelagic fish resources in the Java Sea, especially Banyuwangi, are dominated by catches of Decapterus sp, which are mostly landed at the UPT of the Coastal Fisheries Port Muncar. This study aimed to provide information on CPUE trends (Catch Per Unit Effort) and the seasonality of flying fish in the Coastal Fisheries Port Muncar as an effort to encourage effective and sustainable in using of fishery resources. The method used in this study was a survey. The research data was obtained through secondary data including time series of the production of catches of flying fish, and trip data of purse seine, payang and bagan from 2015 to 2019 collected by Coastal Fisheries Port Muncar. The analysis used the CPUE analysis by comparing catches to capture attempts. The results showed, from 2015 to 2019, the highest CPUE of flying fish occurred in 2019, amounting to 13 365.5 kg/trip, while the lowest CPUE occurred in 2016 amounted to 1 050.6 kg/trip. The trend of CPUE flying fish from 2015 to 2019 experienced a fluctuating increase with an average of 7 327.5 kg/trip. A good season for flying fishing occurs from May to December, and shares a peak in December, while famine occurs in January.
Analysis of Snakehead Fish (Channa striata) Cultivation Business in Peatland, Dadahup Village, Kapuas Regency, Indonesia Khairul Anwar; Muhammad Faiq Naufal; Hany Handajani; Soni Andriawan
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 3 No. 2 (2020): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v3i2.13409

Abstract

Snakehead fish (Channa striata) is a popular freshwater commodity with high market demand in Indonesia, especially in Central Kalimantan. Kapuas Regency, there is a group of farmers who utilize the productivity of peatland to be used as a medium of cultivation of snakehead fish through a biological approach. This research aims to analyze the efforts of members of the fish cultivation group Bahtera Handal Dadahup (BHD), Kapuas Regency based on business analyze to determine the net profit value (π), R/C ratio, Payback Period (PP), and Break Even Point (BEP). BHD group obtained a value ranging from Rp 11 935 000 to Rp 46 022 500; R/C > 1, i.e. ranging from 2.96 to 4.29. Payback Period or return period of capital (investment) lasts for 5 to 10 months; and break even point (BEP) values range from Rp 9 318.3 kg-1 to Rp 13 477.7 kg-1. Based on the calculation of the analysis of the business, the business of fish cultivation group Bahtera Handal Dadahup, deserves to be run and developed.
The effectiveness of cellulolytic bacteria on feeds towards the immune response of Tilapia Dony Prasetyo; Anis Zubaidah; Hany Handajani; Emilio Hudin
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 4 No. 2 (2021): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v4i2.20360

Abstract

Tilapia is one of fishery commodities that is very good to be developed in thecultivation sector. However, the tilapia often incurs with the diseases so thatthe cultivators have difficulty in maintaining the health of the tilapia. One ofthe ways to improve the health is by distributing the cellulolytic bacteriaaddition and blood is one of parameters that used to identify the tilapia’shealth. The research method used was an experimental quantitative methodby preserving the fish and giving cellulolytic bacteria on the feeds for 30 days;then, the bloods ware taken to test the immune system. The parameters usedwere 12 parameters, of which parameters involved of 8 main parameters andsupporting parameters. The conclusion of this research is that the besttreatments are in the treatments of P1 because 4 main parameters out of 8main parameters experience an increasement and 6 main parametersexperience highest increasement from all of the treatments. For the P3, thehighest increasement of main immune system is in the leukocytes andphagocytosis activity. Thus, the fish can prevent the outside attacks.
Growth performance of seaweed (Kappapicus alvarezii) in tissue culture with immersion of NPK and TSP fertilizer Sinung Rahardjo; Ratna Suharti; Deby Tiara Sandi; Hany Handajani
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 5 No. 1 (2022): February 2022
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v5i1.20581

Abstract

Kappapicus alvarezii is an important economic commodity of seaweed to be developed in Indonesia. In the context of its development, seeds from tissue culture are needed.The aim of this research is to examine the growth of seaweed by immersing NPK, TSP and combination fertilizers between NPK + TSP on absolute weight, Specific Growth Rate (SGR) and morphological growth including talus length and seaweed talus diameter. This research was conducted from March 2 to May 15, 2020 in the waters of Sumberkencono Village, Wongsorejo Sub-District, Banyuwangi District, East Java. This study uses Kappaphycus alvarezii seaweed seedlings from tissue culture results from the Situbondo Brackish Aquaculture Fisheries Center, and uses treatment fertilizers, namely NPK fertilizer with 15% Nitrogen (N) composition, Phosphate (P2O5) 15%, and Potassium (K) 15% at a dose of 2 g / l and TSP fertilizer with an element composition of Phosphate (P2O5) 18% at a dose of 2 g / l, as well as a combination of NPK fertilizer 1 g / l and TSP 1g / l soaked for 15 minutes and control or without soaking. From the results of the research, obtained weight growth of seaweed by immersing NPK + TSP obtained good growth (Absolute Weight 57.48 grams, SGR 7.21%, talus length 8.5 cm, diameter 6.52 mm), then NPK treatment (Weight Absolute 37.80 grams, SGR 6.13%, Talus length 8.1 cm, diameter 6.43 mm), TSP treatment (Absolute Weight 31.6 grams, SGR 5.69%, Talus length 7.9 cm, diameter 6.36 mm) followed by control (Absolute Weight 24.8 grams, SGR 5.10%, Talus length 7.6 cm, diameter 6.32 mm).
Optimation of Nitrogen and Phosphorus in Azolla Growth as Biofertilizer Handajani, Hany
Makara Journal of Technology Vol. 15, No. 2
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Nitrogen is one of the most important minerals for living organisms. Azolla is known as a small water fern which can fix nitrogen through the symbiotic association with the blue green algae Anabaena. Experimental studies were carried out in the glass house, investigating the growth of Azolla using different levels of nitrogen and phosphorus in the media. The experiment used 12 treatments with 3 levels of nitrogen (0, 5, and 10 mg atom/L) and 4 levels of phosphorus (0, 10, 20, and 30 mg atom/l) in a factorial design with 3 replicates. The results show that the highest biomass for fresh weight (13.28 g), dry weight (1,126 g), and the fastest doubling time (7.71 days) were found in combination of 10 mg atom/L N and 30 mg atom/L P. Meanwhile, the highest protein content was found in combination of 5 mg atom/L N and 20 mg atom/L P. After the third day heterocyst cells in Anabaena could only be found in those two combinations, but the highest heterocyst cells was found in the lower N and P combination similar to the highest protein content. Further experiment is suggested to introduce Azolla as bio-fertilizer for acid water system, such as peat land water.