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Hany Handajani
Universitas Muhammadiyah Malang
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Optimizing the productivity of vaname shrimp fry (Litopenaeus vannamei) through giving Potassium Nitrate (KNO3) to the cultivation of Skeletonema sp Handajani, Hany; Zubaidah, Anis; Nailin, Nila Choirun; Purnomo, Hari
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica, Vol. 12: No. 2 (August, 2025)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v12i2.21451

Abstract

Skeletonema sp. culture its growth is strongly influenced by the nutrients in the environment where it lives, therefore fertilizer is needed in the culture media to support the availability of both macro and micro nutrients. The purpose of this study was to determine the effect of KNO3 administration on the growth and density of Skeletonema sp. cells. and dosage to obtain the best KNO3 fertilizer. This study used a completely randomized design (CRD), consisting of 4 treatments and 3 replications. KNO3 fertilizer dosage treatments included A (100 ppm), B (200 ppm), C (300 ppm), and control K (500 ppm). Parameters measured were specific growth rate and cell density of Skeletonema sp. The results of the study on density showed that the best treatment was treatment K of 269.5 ᵡ 104 cells/mL, followed by C 300 ppm (203.5 ᵡ 104 cells/mL), B 200 ppm (126.08 ᵡ 104 cells/mL), and A 100 ppm (94.75 ᵡ 104 cells/mL). The average specific growth rate produced in each treatment corresponds to the cell density pattern, namely K (0.434/day); C(0.379/day); B(0.279/day); and A (0.220/day). The application of KNO3 fertilizer with different doses had a significant effect on the growth and density of Skeletonema sp. cells. The higher the KNO3 dose, the higher the growth rate and density of Skeletonema sp cells. high too. Keywords: Fertilizer Dose; Kalium Nitrate; Phytoplankton
EFEKTIVITAS EKSTRAK Solanum ferox DALAM MENINGKATKAN RESPONS KEKEBALAN IKAN PATIN JAMBAL (Pangasius djambal) TERHADAP INFEKSI Edwardsiella tarda Handajani, Hany; Sutarjo, Ganjar Adhywirawan; Setyawan, Yhogie Aldi; Wananda, Alif Zidane Juni; Prasetya, Yukis Angga
Jurnal Riset Akuakultur Vol 18, No 2 (2023): (Juni, 2023)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.18.2.2023.93-104

Abstract

Bakteri Edwardsiella tarda yang menginfeksi ikan patin dapat menyebabkan kematian hingga 80%. Terong asam (Solanum ferox) mengandung senyawa antibakteri yang dapat menghambat pertumbuhan bakteri dan dapat membunuh bakteri penyebab infeksi. Tujuan penelitian adalah untuk mengetahui pengaruh ekstrak S. ferox terhadap respons imun ikan patin jambal (Pangasius djambal) serta memperoleh dosis optimum untuk penanggulangan bakteri E. tarda. Metode yang dilakukan adalah eksperimen  dengan  rancangan acak lengkap  (RAL)  dengan  empat perlakuan  dosis ekstrak terong  asam (0, 300, 600, dan 900 ppm) serta kontrol (perendaman antibiotik komersial) dengan tiga ulangan. Ikan uji yang digunakan adalah ikan patin jambal berukuran 14-16 cm sebanyak 150 ekor. Perlakuan diberikan dengan perendaman ikan uji dalam larutan uji selama 7 hari. Parameter uji antara lain gejala klinis, total eritrosit, total leukosit, diferensial leukosit, aktivitas fagositosis, prevalensi, pertumbuhan bobot mutlak, dan  kelangsungan hidup. Hasil penelitian menunjukkan bahwa perendaman ikan patin jambal dalam larutan ekstrak S. ferox meningkatkan respons imun ikan patin jambal yang terinfeksi E. tarda..  Hasil penelitian ini menyimpulkan bahwa ekstrak S. ferox dosis 600 ppm secara efektif meningkatkan aktifitas fagositosis yang kemudian mengoptimalkan respons imun dan profil darah ikan patin jambal. Infection of Edwardsiella tarda bacteria in farmed catfish could lead to mass mortality of up to 80% of the fish. Sour eggplant (Solanum ferox) contains antibacterial compounds which are capable of inhibiting the growth of bacteria and can kill infectious bacteria. The study aimed to determine the effect of S. ferox extract on the immune response of catfish (Pangasius djambal) and obtain its optimum dose to control the infection of E. tarda. The study used an experimental trial in which treatments were arranged in a completely randomized design (CRD) consisting of four treatment doses of sour eggplant extract (0, 300, 600, and 900 ppm) and control (immersion of commercial antibiotic) with three replicates. The test fish used were 150 catfish with a length of 14-16 cm. The treatments were applied by soaking the test fish in the test solutions for 7 days. The measured parameters included clinical symptoms, total erythrocytes, total leukocytes, leukocyte differential, phagocytic activity, prevalence, absolute weight growth, and survival. The results showed that the immersion of catfish in S. ferox extract solution increased the immune responses of catfish infected by E. tarda.  The results of this study concluded that S. ferox extract at a dose of 600 ppm effectively increases phagocytic activity which then optimizes the immune response and blood profile of catfish.
EFIKASI VAKSIN Aeromonas hydrophila TERHADAP IMUNITAS IKAN LELE SANGKURIANG (Clarias gariepinus) DENGAN METODE INFILTRASI HIPEROSMOTIK Zubaidah, Anis; Sari, Yussandra Khartika; Hastuti, Sri Dwi; Handajani, Hany
Jurnal Riset Akuakultur Vol 19, No 1 (2024): (Maret 2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.1.2024.31-44

Abstract

Kendala yang sering dialami pembudidaya ikan lele salah satunya yaitu serangan motile Aeromonas septicemia (MAS). Vaksinasi melalui perendaman merupakan cara yang efektif untuk meningkatkan sistem imun pada tubuh ikan lele, namun kurang memberikan hasil yang optimal sehingga perlu adanya penambahan metode infiltrasi hiperosmotik untuk memaksimalkan penyerapan vaksin. Penelitian ini bertujuan untuk menentukan kisaran salinitas yang baik dalam memaksimalkan penyerapan vaksin Aeromonas hydrophila pada ikan lele. Penelitian ini menggunakan metode eksperimental rancangan acak lengkap dengan lima taraf perlakuan dan tiga kali ulangan, antara lain kontrol negatif (Kn), kontrol positif (Kp), perendaman salinitas 3 ppt (P1), perendaman salinitas 6 ppt (P2), dan perendaman salinitas 9 ppt (P3) pada ikan lele berukuran 12-15 cm. Parameter yang diamati antara lain titer antibodi, relative percent survival, survival rate (SR), total eritrosit, total leukosit, kualitas air, dan gejala klinis. Hasil penelitian menunjukkan nilai tertinggi yaitu pada P2 (6 ppt) dengan nilai titer antibodi sebesar 8,0 ± 0,0, relative percent survival 100%, survival rate 100%, dan total eritrosit 2,80 x 106 sel mm-3, namun total leukosit pada P2 (6 ppt) menunjukkan nilai terendah karena leukosit melawan serangan patogen sehingga jumlah sel menurun. Disimpulkan bahwa perendaman dalam salinitas 6 ppt merupakan salinitas terbaik pada ikan lele dan berbeda nyata (P<0,05) dengan perlakuan lainnya karena proses penyerapan vaksin terjadi secara maksimal sehingga dapat meningkatkan sistem imun ikan lele.One of the obstacles often experienced by catfish farmers is attacks by the motile Aeromonas septicemia (MAS). Vaccination through immersion is an effective way to improve the immune system in the body of catfish, but it does not provide optimal results so it is necessary to add a hyperosmotic infiltration method to maximize vaccine absorption. This study aimed to determine an optimum salinity range to maximize the absorption of the Aeromonas hydrophila vaccine in catfish. This study used a completely randomized design experimental method with five treatment levels and three replications, including negative control (Kn), positive control (Kp), 3 ppt salinity immersion (P1), 6 ppt salinity immersion (P2), and 9 ppt salinity immersion (P3) in catfish sizing 12-15 cm. The parameters observed included antibody titer, relative percent survival, survival rate (SR), total erythrocytes, total leukocytes, water quality, and clinical symptoms. The results of the study showed that the highest value was at P2 (6 ppt) with an antibody titer value of 8.0 ± 0.0, relative percent survival 100%, survival rate 100%, and total erythrocytes 2.80 x 106 cells mm-3, while total leukocytes in P2 (6 ppt) showed the lowest value because leukocytes fought against pathogen attacks so that the number of cells decreased. It was concluded that immersion in 6 ppt salinity was the best salinity for catfish and was significantly different (P<0.05) from other treatments because the vaccine absorption process occurred optimally so that it could improve the catfish's immune system.
PEMBERDAYAAN MASYARAKAT KELOMPOK PEMBUDIDAYA IKAN “SUKSES MAJU BERSAMA” MELALUI PRODUKSI PAKAN IKAN MANDIRI DAN MANAJEMEN KUALITAS AIR DI KARANGBESUKI KECAMATAN SUKUN KOTA MALANG Ganjar Adhywirawan Sutarjo; Hany Handajani
Jurnal Pengabdian Perikanan Indonesia Vol 1 No 2 (2021): Jurnal Pengabdian Perikanan Indonesia
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppi.v1i2.114

Abstract

Abstrak Kelompok pembudidaya ikan merupakan suatu wadah organisasi masyarakat pembudidaya ikan yang terbentuk dan tumbuh atas dasar adanya kepentingan bersama dengan rasa saling percaya, dalam rangka memanfaatkan sumberdaya alam, dana, untuk meningkatkan kesejahteraan anggota. Salah satu kelompok pembudidaya ikan yang berupaya mengembangkan kegiatan usahanya adalah kelompok pembudidaya ikan “Sukses Maju Bersama” yang terletak di kelurahan Karangbesuki Kecamatan Sukun Kota Malang, terbentuk pada tahun 2016. Kendala yang dihadapi dalam pengembangan usaha kelompok adalah belum diterapkannya manajemen budidaya ikan secara baik, semakin meningkatnya harga pakan ikan buatan pabrik dan pengelolaan kualitas air yang masih belum tepat. Kondisi mitra tersebut, mendapat perhatian oleh Tim Jurusan Perikanan Universitas Muhammadiyah Malang melalui program pengabdian masyarakat internal (PPMI). Tujuan dari pelaksanaan kegiatan pengabdian ini adalah untuk meningkatkan pengetahuan dan ketrampilan mitra terkait penerapan standar cara budidaya ikan yang baik (CBIB), produksi pakan ikan mandiri berbasis bahan baku lokal, dan penerapan pengelolaan kualitas air. Kegiatan PPMI ini, menggunakan metode pelatihan dan pendampingan manajemen atau cara budidaya ikan yang baik, kegiatan dilaksanakan selama satu tahun. Hasil yang diperoleh dari program pengabdian yaitu mitra mampu meningkatkan sistem budidaya ikan secara secara intensif melalui penerapan CBIB, mitra mampu memproduksi pakan ikan mandiri berbasis bahan baku lokal diwilayah mitra diantaranya melalui pemanfaatan limbah ampas tahu, dan silase sebagai bahan pakan, serta mitra dapat menerapkan teknik pengelolaan atau manajemen kualitas air melalui penerapan probiotik. Berdasarkan evaluasi bersama, alih teknologi dapat diterima secara baik oleh mitra dan mampu meningkatkan kapasitas produksi budidaya ikan di Kelompok pembudidaya ikan “Sukses Maju Bersama” Sukun Kota Malang.
UPAYA PENINGKATAN KEMANDIRIAN PANGAN DI PANTI ASUHAN PETERMAS MELALUI BUDIDAYA IKAN LELE SISTEM AKUAPONIK Hasana, Imroatin; Ubaidillah, Muhammad Khoiri; Azzahra, Aufa Farra; Trisdayanti, Ike; Puspandari, Lely Ayu; Handajani, Hany
Jurnal Abdi Insani Vol 11 No 1 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i1.738

Abstract

The community service activity involves empowering the community of students at the Petermas Orphanage through the cultivation of catfish using the Mutiara aquaponic system, aiming for food self-sufficiency. This activity is motivated by several issues, including the lack of nutritional needs in the form of animal and plant proteins, irregularity in donors, and limited entrepreneurial skills, leading to insufficient provisions. Additionally, there are unused and neglected concrete ponds due to the limited knowledge of the students and administrators regarding cultivation. The main goal of this program is to address all the challenges faced by the Petermas Orphanage. Furthermore, the program aims to enhance the skills and knowledge of students and administrators in aquaculture. This program is conducted offline and begins with awareness and training activities, the installation of aquaponic ponds, soaking and settling of cultivation water. Subsequently, catfish seed dispersal, aquaponic vegetable planting, and the construction of aquaponic frameworks take place. Following that, there is monitoring, aquaponic catfish harvesting, and activity evaluation. This community service project has achieved positive and satisfactory results. The harvest is highly satisfying, with fresh water spinach and pokcoy vegetables in good condition. The catfish, raised under these conditions, are of high quality and the appropriate size for consumption. One kilogram of catfish contains 7-8 fish, measuring between 20-30 cm, with a weight of 60-85 grams. The PKM-PM program can achieve food self-sufficiency, meet the needs for animal and plant protein, and create new entrepreneurship opportunities for the Petermas Orphanage.
Effectiveness of kepok banana stem extract (Musa Acuminata) on the immune response of catfish (Clarias gariepinus) infected with Aeromonas hydrophila Hany Handajani; Anis Zubaidah; Dina Izzah Kamila
Depik Vol 13, No 3 (2024): DECEMBER 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.3.34892

Abstract

Catfish have high economic value, fast growth, and are easy to cultivate. This is the reason why catfish cultivation is increasing. However, there are still obstacles in the cultivation process, namely the threat of disease, one of which is the Motile Aeromonas Septicaemia (MAS) disease caused by Aeromonas hydrophila bacteria. The countermeasure can be done by using active compounds found in plants, including banana stems. This study aimed to determine the effectiveness of giving banana stem extract to carp culture media and treating A. hydrophila disease. This research used an experiment with a completely randomized design. The treatments tested were the addition of banana stem extract at a dose of 5% (treatment A), 10% (treatment B), 15% (treatment C), and antibiotics (treatment K-), and without extract treatment (treatment K+). Each treatment was repeated three times. The results showed that the administration of banana stem extract significantly affected the fish's erythrocytes, leukocytes, and blood glucose. Leukocyte differential observation showed that kepok banana stem extract had a significant effect on eosinophils, lymphocytes, and neutrophils but no significant effect on monocytes. In analyzing fish blood, the optimal values were 7.640.190a cells/mm3 for leukocytes, 3.600.116d cells/mm3 for erythrocytes, 656.028b mg/dl for glucose, 74.670.577d% for lymphocytes, 5.670.577a% for neutrophils, and 00a% for eosinophils. The results indicate that treatment B with 10% kepok banana stem extract was the most effective in administering the extract to catfish infected with A. hydrophila bacteria.Keywords:Dumbo catfishLeukocytesErythrocytesGlucoseMusa AcuminataAeromonas hydrophila
Physiological response of Cakul Wader Fish (Puntius Binotatus) fed with Pumpkin Seed flour (Cucurbita Moschata Durch) feed Rivaldy Rama Budiman; Hany Handajani; Dony Prasetyo
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 7 No. 2 (2024): August
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v7i2.12356

Abstract

The physiological condition and reproductive performance of fish are strongly influenced by feed quality and nutritional supplementation. Yellow pumpkin seed flour (*TBLK*) contains various nutrients and bioactive compounds that potentially improve fish physiological responses, growth, and reproductive development. Therefore, this study aimed to evaluate the effect of TBLK supplementation in feed on the physiological responses of cakul wader fish, including the Hepatosomatic Index (HSI), Viscerosomatic Index (VSI), Gonad Maturity Index (GMI), and survival rate. This study employed an experimental method using a Completely Randomized Design (CRD) with four treatments and three replications. The treatments consisted of commercial feed without supplementation (control), commercial feed supplemented with 2.5% TBLK, 5% TBLK, and 7.5% TBLK, each combined with 3% CMC as a binder. Fish with an average body weight of 4.9 ± 1.0 g were stocked at 25 fish per aquarium containing 25 L of water and reared for 40 days. Feeding was conducted twice daily using paste-form feed. The results showed that TBLK supplementation affected the physiological responses of cakul wader fish. The best performance was obtained at the 5% TBLK supplementation level, which produced the highest Gonad Maturity Index (0–5.81%) and survival rate (86.67%), while maintaining favorable HSI (1.29–1.93%) and VSI (2.44–3.93%) values. Lower survival rates were observed in the 2.5% (68%) and 7.5% (66.67%) treatments. In conclusion, supplementation of 5% TBLK in feed was the optimal dose for improving physiological condition, gonad maturation, and survival rate of cakul wader fish
Effectiveness of kepok banana stem extract (Musa Acuminata) on the immune response of catfish (Clarias gariepinus) infected with Aeromonas hydrophila Hany Handajani; Anis Zubaidah; Dina Izzah Kamila
Depik Vol 13, No 3 (2024): DECEMBER 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.3.34892

Abstract

Catfish have high economic value, fast growth, and are easy to cultivate. This is the reason why catfish cultivation is increasing. However, there are still obstacles in the cultivation process, namely the threat of disease, one of which is the Motile Aeromonas Septicaemia (MAS) disease caused by Aeromonas hydrophila bacteria. The countermeasure can be done by using active compounds found in plants, including banana stems. This study aimed to determine the effectiveness of giving banana stem extract to carp culture media and treating A. hydrophila disease. This research used an experiment with a completely randomized design. The treatments tested were the addition of banana stem extract at a dose of 5% (treatment A), 10% (treatment B), 15% (treatment C), and antibiotics (treatment K-), and without extract treatment (treatment K+). Each treatment was repeated three times. The results showed that the administration of banana stem extract significantly affected the fish's erythrocytes, leukocytes, and blood glucose. Leukocyte differential observation showed that kepok banana stem extract had a significant effect on eosinophils, lymphocytes, and neutrophils but no significant effect on monocytes. In analyzing fish blood, the optimal values were 7.640.190a cells/mm3 for leukocytes, 3.600.116d cells/mm3 for erythrocytes, 656.028b mg/dl for glucose, 74.670.577d% for lymphocytes, 5.670.577a% for neutrophils, and 00a% for eosinophils. The results indicate that treatment B with 10% kepok banana stem extract was the most effective in administering the extract to catfish infected with A. hydrophila bacteria.Keywords:Dumbo catfishLeukocytesErythrocytesGlucoseMusa AcuminataAeromonas hydrophila
DIFFERENT USE OF PROBIOTICS ON THE GROWTH AND WATER QUALITY OF VANNAMEI SHRIMP (Litopenaeus vannamei) Achmad Hidayah Guantoro; Hany Handajani; Ganjar Adhywirawan Sutarjo
IJOTA (Indonesian Journal of Tropical Aquatic) Vol. 6 No. 1 (2023): February
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/ijota.v6i1.18815

Abstract

Vannamei shrimp (Litopenaeus vannamei) cultivation in the last 5 years has become excellent. Water quality is a determining factor for the success of cultivation. The purpose of this study was to determine the effect of using different probiotics on the growth and water quality of vannamei shrimp ( Litopenaeus vannamei ). This research was conducted at UPT. BAPL Bangil and UPT. Labkeskanling Pasuruan was held on 29 June – 29 July 2021. This study used a completely randomized design with 4 treatments and 3 repetitions with a dose of Control, As-One (0.009%), GDM (0.09%), and Epicin-D (0 ,09%). The research results were analyzed using ANOVA. The data obtained from the variance was then further tested with the BNT test. Treatment 4 (Epicin-D) had the highest value (absolute weight 28.24 gr, absolute length 14.71 cm, specific growth 7.88 gr) and treatment 1 (control) had the lowest value (absolute weight 20.73 gr, absolute length 8.92 cm, specific growth 6.11 gr). The results of observations of water quality during the study were temperature 27.70 oC – 28.30 oC, pH 7.50 – 7.52, dissolved oxygen 5.44 mg/l – 5.50 mg/l, salinity 9.88 ppt – 9.94 ppt, Ammonia 0.25 mg/l, nitrite 0.91 mg/l, and nitrate 55.21 mg/l. TPC results for Treatment 1 (2.5 x 108 Cfu/ml and 2.9 x 109 Cfu/ml), Treatment 2 (2.5 x 108 Cfu/ml and 2.4 x 109 Cfu/ml), Treatment 3 (2.5 x 108 Cfu/ml and 8.9 x 108 Cfu/ml), and Treatment 4 (2.5 x 108 Cfu/ml and 2.9 x 109 Cfu/ml). Based on the results of this study, it can be concluded that the observed parameters (absolute weight growth, absolute length, and specific growth rate) were significantly different but vannamei shrimp survival was not significantly different.
Co-Authors Achmad Hidayah Guantoro Anis Zubaidah Arianto, Doddy Astuti, Triana Indri Azzahra, Aufa Farra Baihaqi, Ahmad Uzair David Hermawan David, Hermawan Deby Tiara Sandi Dessy Amitha Kusumaningdyah Devi Purwinda Dewi Liesnoor Setyowati Dina Izzah Kamila Dony Prasetyo Dwi Hastuti Dyah Ayu Puspita Eko Handayanto Emilio Hudin Fahimuridlo, Muhammad Fahreza, Mohammad Adam Fathimatuz Zahroh, Fathimatuz Fuhaira, Iqbal Ganjar Adhy Wirawan Ganjar Adhy Wirawan, Ganjar Ganjar Adhy Wirawan, Ganjar Adhy Ganjar Adhywirawan Sutarjo Ghifari, Maulana Zaky HARI PURNOMO Hariyadi Hariyadi Hariyadi, Hariyadi Hasana, Imroatin Hermawan, David Heryanto Heryanto Irawan, Phobie Yolasica Jasmine, Andrea Sherina Khairul Anwar Masitoh Masitoh Mia Setiawati Mochammad Noval Muhammad Fahimuridlo Muhammad Faiq Naufal Nailin, Nila Choirun Noval, Mochammad Nur Subekhi, ST.MT Nur Subekhi, ST.MT, Nur Prasetya, Yukis Angga Puspandari, Lely Ayu Putri Ramadhani, Putri Rahayu Relawati Rahmania, Wanda Ratna Suharti Rivaldy Rama Budiman Riza Rahman Hakim Sari, Yussandra Khartika Setyawan, Yhogie Aldi Sinung Rahardjo Soni Andriawan Sri Dwi Hastuti Sujono Sujono Sulis Puji Rohmah Taqwa, Brilian Amanat Tatag Budiardi Titan Aji Wiratama Tri Makmun Arifin, S.Pi Tri Makmun Arifin, S.Pi, Tri Makmun Triana Indri Astuti Trisdayanti, Ike Ubaidillah, Muhammad Khoiri Wahyudi, Moh. Ilham Wananda, Alif Zidane Juni WIDANARNI WIDANARNI Yoga Yuniadi