safrina dyah hardiningtyas
1Departemen Teknologi Hasil Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor, Jalan Agatis, Bogor 16680 Jawa Barat Telepon (0251) 8622909, 8622907, Faks. (0251) 8622907

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Journal : Journal of Marine and Coastal Science

Antimicrobial Activity of Nanochitosan Shrimp Shell as a Hands Sanitizer Safrina Dyah Hardiningtyas; Dona Fradilla Bahri; Pipih Suptijah
Journal of Marine and Coastal Science Vol. 11 No. 1 (2022): FEBRUARY
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v11i1.33821

Abstract

Chitosan is a biopolymer that can be extracted from crustaceans, such as shrimp shells. Chitosan is widely used in various fields, such as pharmaceuticals, food, and cosmetics because it is biodegradable, non-toxic, and has antibacterial activity. Nanochitosan is a dosage form based on nanotechnology which has more reactive properties. The purpose of this study was to characterize chitosan and nanochitosan and evaluate the effectiveness of nanochitosan as an antibacterial in hand sanitizer. The tests carried out included analysis of the functional group of chitosan with FTIR, particle size analysis with particle size analyzer, morphological analysis of nanochitosan with SEM, and analysis of the effectiveness of hand sanitizers. The test chitosan used had a degree of deacetylation of 85.94%. The particle size distribution of nanochitosan was 140.94 nm with a polydispersion intensity (PDI) of 0.5830. The results of the antibacterial effectiveness test of nanochitosan as a hand sanitizer showed that the higher the concentration of nanochitosan used, the total bacterial colonies would decrease. The concentration of 2000 ppm nanochitosan can reduce 93.4% of bacterial colonies on probandus hands, and this activity is known to be not significantly different from commercial hand sanitizers.
Potency of Cellulolytic Bacteria in Ulva lactuca from Ujung Genteng Beach, Sukabumi, Indonesia Pari, Rizfi Fariz; Hardiningtyas, Safrina Dyah; Supraba, Ranula
Journal of Marine and Coastal Science Vol. 12 No. 2 (2023): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v12i2.46102

Abstract

Fishery Ulva lactuca is a potential green seaweed species that causes green tides and has not been utilized. U. lactuca has the potency to attract cellulolytic bacteria due to its high carbohydrate content. U. lactuca is commonly found on the southern seacoast of Java Island, one of which is Ujung Genteng Beach, Sukabumi, Indonesia. In this research, we investigated the potential of cellulolytic bacteria from U. lactuca. Sources of bacteria are coming from the environment, the surface of the seaweed, and inside the seaweed (endophytes). To be able to determine the origin of the potential bacteria, the total plate count (TPC) was calculated with treatment without washing, washing with distilled water, and washing with a combination of distilled water and alcohol. From these bacteria, cellulolytic activity was tested using CMC media and morphological characterization. The most bacteria were found in the treatment without washing, as much as 7.48 Log Cfu/ml. The washing process reduced the TPC to 5.88 and 5.7 Log Cfu/ml in the treatment of washing with distilled water and washing with a combination of distilled water and alcohol, so that endophytic bacteria were obtained. The bacterial isolate with the highest cellulolytic potential was UW 1(1)1 5A1 from the non-washing treatment with a cellulolytic index of 2.25. These Gram-positive bacteria were in the form of filamentous lobates with a matte cream color with cocci-shaped cells. Meanwhile, the potential endophytic bacteria were Gram-positive coccus bacteria UW 3(1)1.2 with a cellulolytic index of 0.85. Both isolates have the potency to degrade cellulose.