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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika Research Journal of Life Science Jurnal Penelitian Pendidikan IPA (JPPIPA) Biopendix: Jurnal Biologi, Pendidikan dan Terapan
Nunung Harijati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics

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Effect of Dietary Amorphophallus sp From East Java on LDL-C Rats (Rattus novergicus Wistar Strain) Nunung Harijati; Sri Widyarti; Rodiyati Azrianingsih
Journal of Tropical Life Science Vol. 1 No. 2 (2011)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

One of indication of obesity is high LDL-C . Obesity has serious risk to health, it can cause heart disease and stroke. Effort to lower obesity using drugs have significant side effects such as insomnia, increased blood pressure, dry mouth and so forth. Therefore using natural products that contain glucomannan to reduce obesity and LDL-C is good choise. Glucomannan in the global market derived from Amorphophallus konjac. In this study, we used glucomannan from Amorphophallus endemic East Java to reduce fattened Wistar rats. Amorphophallus that used include Amorphophallus muelleri, A.variabilis (variant: Brongkos 32; Brongkos 5; Wonorejo, Brangsi) and Amorphophallus campanulatus (Var Selopuro). Amorphophallus diet was given to white rats of Wistar strain that previously fattened using a mixture of cholesterol and lard, in addition to PARS (Chicken Feed Race Super) as a basic food. Amorphophallus konjac was used as control. Measurement of LDL-C was conducted in four different time points, namely 1). Prior to dietary cholesterol, 2). 24 days after the dietary cholesterol, 3) 11 days after Amorphophallus (+cholesterol) diet, 4). 25 days after Amorphophallus (+cholesterol) diet. The results showed that the diet of A.variabilis potentially lowered blood cholesterol levels for their respective 22.98%, 5.85% and 7.37% for consecutive variant Brongkos 32; Brongkos 5; Wonorejo. Diet from A.campanulatus and A.konjac had not been able to reduce cholesterol to the end of observation (25 days).Keywords: LDL-C, A.variabilis, A.muelleri, A.campanulatus, A.konjac
Effect of Ethyl Methane Sulfonate on Bulbil Explant Growth and In Vitro Shoot Formation in Porang (Amorphophallus muelleri Blume) Febriyanti, Vera; Waluyo, Budi; Harijati, Nunung; Martasari, Chaireni; Widoretno, Wahyu
The Journal of Experimental Life Science Vol. 14 No. 2 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.02.04

Abstract

Porang is one of the plant commodities with high economic value due to its high glucomannan content in the tubers. Currently, the global demand for porang tubers continues to rise, but domestic production has not yet met this demand due to the limited supply of superior porang seeds. Mutation breeding with Ethyl Methane Sulfonate (EMS) can be one of the alternative methods for developing superior porang to enhance tuber production. This study aims to determine the response of porang bulbil explants to EMS mutagen treatment in vitro. The research used a Randomized Complete Block Design consisting of six EMS concentrations (0, 0.02, 0.04, 0.06, 0.08, and 0.1%). The research stages included pre-culture of bulbil explants on MS + BAP 3 mg.L-1 + NAA 0.1 mg.L-1 for two weeks in dark conditions and EMS mutagen treatment on bulbil cultures for four weeks. The results showed that adding EMS mutagen to the medium for four weeks caused the explants browning, inhibited shoots forming, and decreased the fresh weight and growth index. The higher the EMS concentration in the medium, the increased percentage of browning explants and reduced explant growth and shoot formation. The EMS concentration (≥0.06%) inhibited explant growth and shoot formation. While EMS concentration (0.02-0.04%) increased explant growth, shoot formation was not significantly different from control, even though there was an increase. Up to four weeks of culture, there was no explant death, so LD50 is not yet known. The LD50 EMS can also be calculated by explant browning and shoot proliferation. Based on explant browning and shoot proliferation, the LD50 EMS in porang bulbil explants was 0.07% and 0.09%. The EMS concentration and length of culture age need to be increased to obtain an LD50 survival rate. Keywords: Amorphophallus muelleri Blume, bulbil, EMS, In Vitro mutagenesis.
The Relationship of Chemical Compounds and Crystal Development in Porang Tuber (Amorphophallus muelleri Blume) Chairiyah, Nurul; Harijati, Nunung; Mastuti, Retno
The Journal of Experimental Life Science Vol. 13 No. 1 (2023)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2023.013.01.01

Abstract

Chemical compounds are presumed to interact with each other and potentially affect the crystal development of porang tubers. This study focused on glucomannan and calcium oxalate (CaOx) as chemical compounds found in porang. Crystal development was analyzed by microscopic observation. The tuber tissue was used for microscopic slides and was harvested two weeks before the plants lost their leaves, when the plants lost their leaves, and two weeks after the plants lost their leaves. The CaOx and glucomannan content was measured using a modified extraction method. The increasing of CaOx content tends to increase the number of CaOx crystals. The analysis showed that the crystal density could affect the increasing of tuber weight possibility. There was a development process in raphide crystals that showed by different sizes at three harvest times, which the others were not. The results also indicated that glucomannan might influence the crystal density and the CaOx content. It can be concluded that glucomannan can potentially induce CaOx synthesis and crystal count increase. Keywords: Caox, Crystal, Development, Glucomannan, Interact, Porang.
The Effect of Basal Medium on Callus Induction and Plant Regeneration in Anther Culture of Rice (Oryza sativa L.) Iriani, Yoanita Fadlilah; Prayantini, Dian Catur; Nahampun, Hartinio Natalia; Mastuti, Retno; Harijati, Nunung
Jurnal Penelitian Pendidikan IPA Vol 11 No 8 (2025): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i8.11884

Abstract

Anther culture is an effective tissue culture technique for rapidly producing doubled haploid (DH) plants, significantly shortening the breeding cycle by 2-3 years compared to conventional methods. This study aimed to evaluate the effects of callus induction basal medium (B5 Gamborg and N6 Chu) and subsequent plant regeneration in both Japonica and Indica rice genotypes. The experiment was conducted using a completely randomized design (CRD) with two treatment factors. The first factor was genotype (G), comprising Nipponbare (G1), RCKJ 05 (G2), RCKJ 10 (G3), RCKJ 15 (G4), RCKJ 25 (G5). The second factor was the basal medium (M), consisting of N6 Chu medium (M1) and B5 Gamborg medium (M2). Each treatment combination (genotype x medium) was replicated three times, with one Petri dish (containing 125 anthers) per replicate. Parameters observed included callus induction frequency (CIF), green plantlet regeneration (RGP), and albino plantlet regeneration (RAP). The results demonstrated that both genotype and the basal medium for callus induction significantly influenced callus induction frequency and green plantlet regeneration.
Co-Authors Budi Waluyo Chaireni Martasari Dian Flowrenzhy Estri Laras Arumingtyas Febriyanti, Vera Flowrenzhy, Dian Hermalina Sinay Iriani, Yoanita Fadlilah Nahampun, Hartinio Natalia Nurul Chairiyah Prayantini, Dian Catur Retno Mastuti Retno Mastuti Rodiyati Azrianingsih Rodiyati Azrianingsih Savitri Nurlaila Serafinah Indriyani Sri Widyarti Wahyu Widoretno Wahyu Widoretno Yasminatul Khoiroh Yasminatul Khoiroh