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All Journal Jurnal Veteriner Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Kedokteran Hewan
Muharam Saepulloh
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Zoonotic Diseases Associated with Encephalitis Darminto .; Sjamsul Bahri; Muharam Saepulloh
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 9, No 1 (1999)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.897 KB) | DOI: 10.14334/wartazoa.v9i1.728

Abstract

Outbreak of encephalitis, which killed more than 80 people in Malaysia, invited public attention throughout the world, especially  stakeholders  in  animal husbandry  and  veterinary  practices.  The  disease  was initially  diagnosed as Japanese-B- encephalitis (JE) which was transmitted to human from infected pigs. Recently, the causal agent of the outbreak has been identified as morbilivirus which was called Hendra-like Virus or Nipah Virus. Indonesia as neighboring country to Malaysia needs to take anticipation to prevent the occurrence of similar outbreak. For anticipation, it is required to understand all diseases, which cause encephalitis, especially the zoonotic diseases. From many infectious diseases of animal, only 17 diseases which are able to cause encephalitis, 9 of them are zoonotic diseases: Eastern equine encephalomyelitis (EEE), Western equine encephalomyelitis   (WEE),   Venezuelan   equine   encephalomyelitis   (VEE),   Japanese-B-encephalitis   (JE),   Murray   valley encephalitis (MVE), Louping-ill, Rabies and Equine morbilivirus (EMV) and Nipah Virus. The viral agents and the mode of transmission to human are discussed in this publication. All those encephalitic zoonoses, except Rabies and JE, are exotic to Indonesia. So it is required to keep the free status of Indonesia to those diseases by strict quarantine measures. All imported animals to Indonesia should be confirmed free from those diseases based on the laboratory examination.   Key words: Encephalitis, zoonosis, nipah virus
Real Time Polymerase Chain Reaction : Perangkat Diagnostic Alternatif untuk Melacak Virus Nipah (REAL TIME POLYMERASE CHAIN REACTION : AN ALTERNATIVE DIAGNOSTIC TOOL TO DETECT NIPAH VIRUS) Indrawati Sendow; Atik Ratnawati; Raden Mas Abdul Adjid; Muharam Saepulloh
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.356 KB)

Abstract

Nipah is a dangerous zoonotic disease with a high social, economical and psychological impact. Fruitbat Pteropus sp. is one of the nipah virus  reservoir host. As the virus is categorized as a dangerous zoonoticdisease that cause fatal in human, all works related to live virus should be conducted in a laboratory withBSL4 facilities. The detection of nipah virus using real time PCR to replace virus isolastion can thereforebe conducted in a laboratory without BSL4 facilities. The results was further  confirmed at referencelaboratory at   Australian Animal Health Laboratory ( AAHL) Geelong, Australia, indicated that nipahvirus can be detected in saliva of fruit bat P. vampyrus in Medan North Sumatera.
Sensitivitas dan Spesifisitas Nested Polymerase Chain Reaction untuk Mendeteksi DNA Coxiella burnetii (SENSITIVITY AND SPECIFICITY OF NESTED POLYMERASE CHAIN REACTION FOR DETECTION OF COXIELLA BURNETII DNA) Trioso Purnawarman; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu; Agus Setiyono; Muharam Saepulloh
Jurnal Veteriner Vol 13 No 1 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.261 KB)

Abstract

Sensitivity and specificity of nested polymerase chain reaction (nested PCR) to detect Coxiella burnetii(C. burnetii) DNA were studied. The primer system which consists of external primers (OMP1 and OMP2)and internal primers (OMP3 and OMP4), was designed from the nucleotide sequence of the com I geneencoding for 27 kDa outer membrane protein and used to specifically amplify a 501 bp and 438 bp fragment.This nested PCR assay was 50 fold more sensitive than that of using PCR external primer only. TheNested PCR has a detection limit as low as 300 pg/?l. Specificity studies showed that nested PCR onlydetected C. burnetii DNA and did not happened Brucella abortus, Escherichia coli, Pseudomonas aeruginosaand Campylobacter Jejuni DNA. Nested PCR has high senstively and specificaly diagnostic method of C.burnetii as agent of Q fever disease.
PENGEMBANGAN TEKNIK ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) MENGGUNAKAN ANTIBODI MONOKLONAL UNTUK MENDETEKSI ANTIBODI PENYAKIT BOVINE EPHEMERAL FEVER Indrawati Sendow; R.M. Abdul Adjid; Atik Ratnawati; Muharam Saepulloh
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.824 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2775

Abstract

Penelitian ini bertujuan mengembangkan teknik enzyme-linked immunosorbent assay (ELISA) untuk mendeteksi antibodi terhadap virus bovine ephemeral fever (BEF). Pada penelitian ini dikembangkan uji ELISA langsung (direct ELISA) dan tidak langsung (indirect ELISA) dengan menggunakan antibodi monoklonal (blocking ELISA). Hasil penelitian menunjukkan bahwa uji direct ELISA tidak dapat digunakan dengan baik karena terjadi positif palsu. Uji blocking ELISA bereaksi lebih baik dan dapat dikembangkan lebih lanjut untuk mendeteksi antibodi terhadap penyakit BEF. Dapat disimpulkan bahwa pengembangan teknik deteksi dini terhadap BEF dengan mempergunakan antibodi monoklonal dapat diterapkan dalam upaya pengawasan penyakit dan surveilans.
Co-Authors Agus Setiyono Atik Ratnawati D. Sajuthi D. Setiyaningsih Darminto . Fachriyan Hasmi Pasaribu Helmy Hamid Hope G Rovira I Wayan T. Wibawan I wayan Teguh Wibawan I.W.T. Wibawan Indrawati Sendow Indrawati Sendow Indrawati Sendow NLP I Dharmayanti NLP Indi Dharmayanti Nur Sabiq Assadah R.M Abdul Adjid R.M. Abdul Adjid Raden Mas Abdul Adjid Risa Indriani Sjamsul Bahri Trioso Purnawarman