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Zoonotic Diseases Associated with Encephalitis Darminto .; Sjamsul Bahri; Muharam Saepulloh
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 9, No 1 (1999)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.897 KB) | DOI: 10.14334/wartazoa.v9i1.728

Abstract

Outbreak of encephalitis, which killed more than 80 people in Malaysia, invited public attention throughout the world, especially  stakeholders  in  animal husbandry  and  veterinary  practices.  The  disease  was initially  diagnosed as Japanese-B- encephalitis (JE) which was transmitted to human from infected pigs. Recently, the causal agent of the outbreak has been identified as morbilivirus which was called Hendra-like Virus or Nipah Virus. Indonesia as neighboring country to Malaysia needs to take anticipation to prevent the occurrence of similar outbreak. For anticipation, it is required to understand all diseases, which cause encephalitis, especially the zoonotic diseases. From many infectious diseases of animal, only 17 diseases which are able to cause encephalitis, 9 of them are zoonotic diseases: Eastern equine encephalomyelitis (EEE), Western equine encephalomyelitis   (WEE),   Venezuelan   equine   encephalomyelitis   (VEE),   Japanese-B-encephalitis   (JE),   Murray   valley encephalitis (MVE), Louping-ill, Rabies and Equine morbilivirus (EMV) and Nipah Virus. The viral agents and the mode of transmission to human are discussed in this publication. All those encephalitic zoonoses, except Rabies and JE, are exotic to Indonesia. So it is required to keep the free status of Indonesia to those diseases by strict quarantine measures. All imported animals to Indonesia should be confirmed free from those diseases based on the laboratory examination.   Key words: Encephalitis, zoonosis, nipah virus
PENGEMBANGAN TEKNIK ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) MENGGUNAKAN ANTIBODI MONOKLONAL UNTUK MENDETEKSI ANTIBODI PENYAKIT BOVINE EPHEMERAL FEVER Indrawati Sendow; R.M. Abdul Adjid; Atik Ratnawati; Muharam Saepulloh
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.824 KB) | DOI: 10.21157/j.ked.hewan.v9i1.2775

Abstract

Penelitian ini bertujuan mengembangkan teknik enzyme-linked immunosorbent assay (ELISA) untuk mendeteksi antibodi terhadap virus bovine ephemeral fever (BEF). Pada penelitian ini dikembangkan uji ELISA langsung (direct ELISA) dan tidak langsung (indirect ELISA) dengan menggunakan antibodi monoklonal (blocking ELISA). Hasil penelitian menunjukkan bahwa uji direct ELISA tidak dapat digunakan dengan baik karena terjadi positif palsu. Uji blocking ELISA bereaksi lebih baik dan dapat dikembangkan lebih lanjut untuk mendeteksi antibodi terhadap penyakit BEF. Dapat disimpulkan bahwa pengembangan teknik deteksi dini terhadap BEF dengan mempergunakan antibodi monoklonal dapat diterapkan dalam upaya pengawasan penyakit dan surveilans.