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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science Jurnal Tanaman Industri dan Penyegar Perspektif : Review Penelitian Tanaman Industri JURNAL AGROHITA Asian Journal of Agriculture Akta Agrosia Perspektif, Review Penelitian Tanaman Industri
RITA HARNI
Balai Penelitian Tanaman Industri dan Penyegar
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Immunology & microbiology Industrial & Manufacturing Engineering Veterinary Other

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Volatile organic compounds from Bacillus subtilis BE6 inhibit and lyse mycelial growth of Sclerotium rolfsii HARDIYANTI, SITI; AMARIA, WIDI; RAHAYUNINGSIH, SRI; SUPRIADI, SUPRIADI; WARTONO, WARTONO; HARNI, RITA; KORLINA, ELI; WIDODO, WIDODO; SYABANA, MOHAMAD ANA; WIJAYA, RUSBIANTO
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100123

Abstract

Abstract. Hardiyanti S, Amaria W, Rahayuningsih S, Supriadi, Wartono, Harni R, Korlina E, Widodo, Syabana MA, Wijaya R. 2026. Volatile organic compounds from Bacillus subtilis BE6 inhibit and lyse mycelial growth of Sclerotium rolfsii. Asian J Agric 10 (1): g100123. https://doi.org/10.13057/asianjagric/g100123. Sclerotium rolfsii is a devastating soil-borne pathogen responsible for significant crop losses worldwide, highlighting the need for effective control strategies. Antagonistic bacteria provide an eco-friendly biocontrol alternative for managing such plant diseases. This study evaluated the antifungal efficacy of Volatile Organic Compounds (VOCs) produced by eight bacterial isolates belonging to the genera Bacillus, Serratia, Brucella, and Burkholderia against S. rolfsii under in vitro conditions. Antifungal activity was assessed using a dual-culture assay in divided petri dishes, followed by Scanning Electron Microscopy (SEM) to assess morphological changes, and headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME/GC-MS) for chemical profiling. Among the isolates, indigenous Bacillus subtilis BE6 exhibited the strongest inhibitory effect on S. rolfsii isolate Sc.lb, achieving a growth inhibition rate of 57.2±10.5% compared to the control. Other notable isolates included Bacillus amyloliquefaciens P7 (43.5±7.3%) and Serratia surfactantfaciens S108 (42.7±10.9%). Observation through SEM demonstrated that VOCs from B. subtilis BE6 induce severe hyphal abnormalities, such as shrinkage, wrinkling, and lysis. GC-MS analysis identified 27 VOCs emitted by B. subtilis BE6, with the dominant compounds being cis-2,3-epoxybutane (27.34%), methyl (Z)-N-hydroxybenzenecarboximidate (17.19%), 5-methyl-2-hexanone (14.10%), hexanal (12.46%), and 2,5-dimethylpyrazine (2.89%). These compounds belong to the aldehyde, epoxide, oxime, ketone, and pyrazine groups, all of which are associated with antimicrobial properties. Overall, the results demonstrate that VOCs produced by B. subtilis BE6 effectively inhibit and disrupt the mycelial growth of S. rolfsii, highlighting their potential as eco-friendly biofumigant agents for the managing of soil-borne plant diseases. Further studies are required to validate their efficacy under in vivo and field conditions, as well as to elucidate the individual and synergistic roles of key VOCs.
Co-Authors Amaria, Widi Arifal, Fahkrul Baharuddin Baharuddin Efi Taufik Efi Taufiq Eli Korlina, Eli Handi Supriadi I Ketut Suada Iis Nur Asyiah Imam Mudakir Khaerati Khaerati, Khaerati Lilipaly, Elena F. L. M. Yogi Riyantama Isjoni Mohamad Ana Syabana Reginawanti Hindersah Salsabila, Unik Hanifah Siti Hardiyanti Sri Rahayuningsih Sulyanti, Eri Supramana Supriadi Supriadi Wartono Wartono Widi Amaria Widodo Widodo WIJAYA, RUSBIANTO Yulmira Yanti