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Journal : Makara Journal of Science

Craniofacial Shape of Arfak People Based on Geometric Morphometric Features Kawulur, Elda Irma Jeanne Joice; Suryobroto, Bambang; Budiarti, Sri; Hartana, Alex
Makara Journal of Science Vol. 22, No. 1
Publisher : UI Scholars Hub

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Abstract

Face and cranial (craniofacial) shape is highly specific to the individual; therefore, craniofacial shape is often used to identify individuals and to analyze variability in the human population. Previous studies, consisting only of verbal descriptions, suggested that the cranial shape of the Papuan people was highly variable. Despite their usefulness, verbal descriptions cannot fully demonstrate common and local variation in cranial shape. They also cannot be used to extractthe general trend of variation or to group face shapes based on their similarity. Here we attempt to apply geometric analysis, a method of shape analysis, to measure facial anatomical structural landmarks of Papuan people. The craniofacial shape of Papuan people was constructed from those of Arfak people based on 16 anatomic landmarks on the lateral side. Arfak is one of the traditional Papuan tribes in Manokwari, West Papua Province. Our result showed great variation in craniofacial shapes among the Arfak. The nose, chin, and mandible differed significantly, whereas other parts of the face were relatively stable and showed small variations. These differences reflected variations in the facial growth rate. The high level of diversity thus indicates that some parts of the face have higher plasticity in their growth pattern than others.
Leaf Trichome Morphology of Durio Kutejensis Landraces from Kalimantan Priyanti,; Chikmawati, Tatik; Sobir,; Hartana, Alex
Makara Journal of Science Vol. 19, No. 1
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The leaf trichome morphology of 19 Durio kutejensis (lai) landraces was studied. The observation of cross- and paradermal sections of D. kutejensis leaves showed that all landraces have glabrous leaves on the adaxial surface, while their abaxial surfaces are covered by six trichome types, one glandular (one- or two-celled stalks with a spheroid multicellular glandular head) and five non-glandular (complex peltate, simple peltate, cushioned stellate, flat stellate, and four-armed stellate trichome with a central cushion). All landraces were rarely covered by glandular trichomes. The non-glandular trichomes are varied in type, density, number of layers, diameter, and shape and margin color of the complex peltate among landraces. One landrace comprises three non-glandular trichome types, while the other landraces consist of four or five non-glandular trichome types. The shapes and margin color of complex peltate trichomes of D. kutejensis are the specific characteristics which distinguish this species from the other Durio species, however these characteristics cannot be used to differentiate one D. kutejensis landraces from the other. Therefore, other characteristics need to be explored in order to distinguish one D. kutejensis landraces from the other.
Characteristics and Ideotype Formulation of Pulasan (Nephelium ramboutan-ake) Fruit Landrace from West Java, Indonesia Djuita, Nina Ratna; Hartana, Alex; Chikmawati, Tatik; Dorly,
Makara Journal of Science Vol. 21, No. 2
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Abstract

Pulasan (Nephelium ramboutan-ake) is a unique tropical fruit, but its morphological variations have not been well characterized. This study was conducted to obtain data on the characteristics of pulasan landrace in West Java, Indonesia, and to formulate the ideotype of pulasan with superior characteristics, such as high fruit weight, sweet taste, and thick and easily peelable aril from the seed. Based on the descriptors of rambutan, 61 fruit variants were observed. The results showed that the average fruit weight was 46.0 g, with the highest at 103.5 g and the lowest at 23.1 g. The °Brix level variation of pulasan fruit was 16.8–29.6, and its vitamin C content was 14.0–24.0 mg/100 g. Five variants with superior fruit characters were identified among the sample collections used in the study
ISOLASI FRAGMEN cDNA DARI GEN PENYANDI AKTIN DARI Melastoma malabathricum Hannum, Saleha; Akashi, Kinya; Suharsono, Utut Widyastuti; Hartana, Alex
Makara Journal of Science Vol. 14, No. 2
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Isolation of cDNA Fragment of Gene Encoding for Actin from Melastoma malabthricum. M. malabathricum grows well in acidic soil with high Al solubility, thereby it can be used as a model plant for tolerance to aluminum and acid stresses. Actin is housekeeping gene used as an internal control for gene expression analysis. The objective of this research was to isolate and clone the cDNA fragments of MmACT encoding for actin of M. malabathricum. Total RNA was isolated and used as the template for cDNA synthesis by reverse transcription. Four cDNA fragments of MmACT, called MmACT1, MmACT2, MmACT3, and MmACT4, had been isolated and inserted into pGEM-T Easy plasmid. Nucleotide sequence analysis showed that the size of MmACT1 and MmACT2 is 617 bp, whereas MmACT3 and MmACT4 is 735 bp. The similarity among these four MmACT is about 78%-99% based on nucleotide sequence and about 98%-100% based on amino acid sequence. Phylogenetic analysis based on amino acid sequence showed that at 1% dissimilarity, the MmACT1, MmACT2, MmACT3 and the ACT5 Populus trichocarpha are clustered in one group, while the MmACT4 is grouped with ACT9 P. trichocarpa and ACT1 Gossypium hirsutum, and these two groups are separated from actin group of monocotyledonous plants. The sequence of MmACT fragments were registered in GenBank/EMBL/DDBJ database with accession numbers AB500686, AB500687, AB500688, and AB500689.