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Effectiveness of Aspergillus oryzae fermentation culture to improve digestion of fibrous feeds Darwinsyah Lubis; E Wina; B Haryanto; T Suhargiantatmo
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 2 (2002): JUNE 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.957 KB) | DOI: 10.14334/jitv.v7i2.280

Abstract

Studies regarding the use of living microorganisms as supplement have been done in the last two decades to improve digestion process in the rumen. Many factors affect the use of the supplement, such as ration composition and physiological status of the animals. Materials used in this experiment are Aspergillus oryzae (AO) derived from ‘tauco’ (salty fermented soybean) and soybean sauce processing factories located in Bogor, Cianjur, and Sukabumi districts (15 sources), and also AO culture collections of the Indonesian Res. Inst. for Anim. Prod. (2 strains) and one strain from Indonesian Res. Inst. for Vet. Sci. in Bogor. The fungus was first isolated in potato dextrose agar (PDA), incubated at room temp. (26-300C) for 5 days for purification. The AO from PDA then inoculated into cooked rice enriched with mineral mix, oven-dried (40-450C), then ground and kept in refrigerator as a stock culture for further use. To produce more AO (scaling-up), the culture is inoculated in two basal media, i.e. soybean meal and ‘onggok’ (tapioca processing waste) enriched with mineral mix, dried and ground, then kept in refrigerator as A. oryzae fermentation culture (AOFC). Ground-dried King grass was used for in vitro digestion trials using sheep rumen fluid, which was not supplemented (control) or supplemented with the AOFC (10% w/w) from various sources. Three best AOFC (by origin) were chosen and used for further digestion study. The study was run using 2 (media) x 3 (AOFC origin) factorial experiment based on a completely randomized design and Duncan’s MRT was applied to test differences among treatment means. Preliminary results indicated of the AOFC-SP66, -F172, and -CT4 used, the best AOFC was SP66, as it increased (P<0.05) fiber (NDF) digestion (10.5% better than the control). Total VFA productions were similar, but acetate content in the rumen fluid was lowered (P<0.05), while propionate and butyrate levels were alleviated (P<0.05) by the AOFC-SP66. Ammonia content was not affected by addition of AOFC. No differences were detected on digestion parameters between the two media used for AO cultivation, therefore, it is suggested to use ‘onggok’ for producing AOFC in large scale. VFAs composition pattern suggests that AOFC was more suitably used for meat rather than for milk production. Key words: Fungus, King grass, digestibility, rumen fluid
Technique for preparation of anaerobic microbes: Rodshaped cellulolytic bacteria Amlius Thalib; B Haryanto; Kuswandi .; H Hamid; Mulyani .
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 3 (2001): SEPTEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.62 KB) | DOI: 10.14334/jitv.v6i3.234

Abstract

Preparation of anaerobic-rod cellulolytic bacteria with coating technique has been conducted. Steps of the processes involved were cultivation, coating, evaporation, and drying. Coating agent used was Gum Arabic, and drying techniquesconducted were freeze drying and sun drying. pH of culture media was firstly optimized to obtain the maximal population ofbacteria. Both coated and uncoated preparates were subjected to drying. Morphological and Gram type identifications showed that uncoated preparate dried with freeze drying is not contaminated (ie. all bacteria are rod shape with Gram-negative type) while the one dried with sun drying is not morphologically pure (ie. containing of both rod and coccus shapes with Gram negative and positive). The coated preparates dried by both freeze and sun drying, were not contaminated (ie. all are rods with Gram-negative). The coating and drying processes decreased viability of preparates significantly. However, the decreasing of viability of coated preparate are lower than uncoated preparate (ie. 89 vs. 97%). Total count of bacteria in sun-drying coated preparate are higher (P<0.05) than the uncoated preparate (ie. 3.38 x 1010 vs.  1.97 x 1010 colony/g DM). Activity of sun-drying coated preparate to digest elephant grass and rice straw was higher (P<0.01) than the sun-drying uncoated preparate with the in vitro DMD values were 42.7 vs. 35.5% for elephant grass substrate and 29.3 vs. 24.6% for rice straw substrate. Therefore, it is concluded that coating technique has a positive effects on the preparation of rumen bacteria.   Key words : Anaerobic bacteria, coating, drying
The effects of inorganic and organic Zn, Cu and Mo supplementation to the in vitro digestibility of grass Supriyati .; D Yulistiani; E Wina; H Hamid; B Haryanto
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 1 (2000): MARCH 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.578 KB) | DOI: 10.14334/jitv.v5i1.176

Abstract

The effects of inorganic and organic Zn, Cu and Mo supplementation to the in vitro digestibility of Elephant grass were studied in this trial. The in vitro trial used the TILLEY dan TERRY method modified by VAN SOEST with the incubation of the grass for 48 hours in sheep rumen liquor medium. Mineral added as fonn of organic and inorganic. The treatment of mineral inorganic supplementations were by adding single e1emen Cu, Zn and Mo and its combination. Mineral added was Zn (as chloride and sulphate salts) 5 ppm, Cu (sulphate salt) 0,1 ppm dan Mo (molybdate salt) 5 ppm, with 4 replicates. Meanwhile the organic minerals added were in the proteinate fonns. Parameter measured were in vitro dry matter digestibility (IVDMD), in vitro organic matter digestibility (IVOMD), pH, VF A total and NH3. Statistical analysis was done by using Complete Split splitsplot design. The supplementation increased pH value, VFA total, IVDMD and IVOMD value (P<0,05) and decreased NH3 value. The highest IVDMD and IVOMD values obtained at the mixture supplementation of mineral Zn (as Zn S04), Cu and Mo, from 58,31 became 69.73% and 52.22 became 62.55% respectively for IVDMD and NOMD. pH value increased from 6.48 to 7.05 and ammonia content decreased from 1,17 to 0,14%. The organic mineral supplementation, en-proteinate dan Zn-proteinate showed that the adding of Zn, Zn and Mo, Zn and Cu also the combination of Zn, Cu and Mo, resulted in IVDMD values as 70.29; 69.97, 64.12 and 63.93%. Further more IVDMD value at the supplementation of Cu and the combination of Cu and Mo were 65.08 and 60,49%. It can be concluded that the supplementation of minerals in the form of inorganic or organic could improve the IVDMD values   Key words: Three element, in vitro digestibility, sheep
Molasses protected palm kernel cake as source of protein for young male Ettawah Grade goats Supriyati .; B Haryanto
Jurnal Ilmu Ternak dan Veteriner Vol 16, No 1 (2011): MARCH 2011
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.929 KB) | DOI: 10.14334/jitv.v16i1.630

Abstract

Palm kernel cake has a relatively high protein content, however its degradability in the rumen is high resulting in loosing its function as protein source for ruminant. This experiment was aimed toinvestigate the effect of feeding molasses protected palm kernel cake (BIS-M) on growth of young male Ettawah Grade (Peranakan Etawah = PE) goat. Twenty four (24) PE goats were divided into 4 groups and allocated to respective feeding BIS-M treatments. The treatments were R0 = control (without BIS-M), R1 = 15% BIS-M, R2 = 30% BIS-M and R3 = 45% BIS-M. The concentrate was fed at 400 gh-1d-1 for each individual in all treatment groups, while napier grass (Pennisetum purpureum) was offered ad libitum. The live weight of the goats were between 17-18 kg at the beginning of experiment. Feeding trial was carried out for 14 weeks consisting of 2 weeks for preliminary and 12 weeks for growth performance period. The digestibility study of the nutrient was carried out during the last 7 days of  the experiment. The experiment was arranged in a completely randomized design with 6 replications. Drinking water was available at any time. Feed intake was recorded daily while the body weight was recorded every 2 weeks. The parameters of observation were feed intake, live weight gain, nutrient digestibility and feed conversion ratio. The results indicated that the dietary treatments affected the intake and digestibility of nutrients, average daily gain and feed conversion ratio (P < 0.05). The total feed dry matter intakes were 599,30; 620,74; 690,19 and 740,04 gh-1d-1 with DM and Protein digestibility of 64.74 and 75.99; 67.47 and 73.05; 70.06 and 73.02; and 72.88 and 72.25% respectively for R0, R1, R2 and R3. The ADG were 42.06; 52.78; 61.90 and 70.24 g; with feed conversion ratio of 14.68; 10.51; 9.08; and 9.85 for R0, R1, R2 and R3. It was concluded that BIS-M can be used as source of protein with optimal utilization level at 30% of the concentrate. Key Words: Palm Kernel Cake, Molasses, Ettawah Grade goats, Performance
Effect of supplementation of Zn-biocomplex in ration on the growth of young sheep Supriyati .; B Haryanto
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 4 (2007): DECEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.174 KB) | DOI: 10.14334/jitv.v12i4.494

Abstract

The effect of supplementation of graded levels of Zn-bio-complex in concentrate on the growth of young sheep was studied. Fourty young Thin Tailled sheeps were devided into 4 groups of Zn-bio complex treatments. Zink-bio-complex was produced by IRIAP’s laboratory. King grass powder (CP = 10.4%) and concentrate (CP=15.5%) were given as a basal diet. King grass powder and drinking water were given ad libitum. The concentrate was given daily at 200 g h-1d-1 supplemented with 0 mg Zn kg-1 of DM (R1), 50 mg Zn kg-1 of DM (R2), 100 mg Zn kg-1 of DM (R3), and 200 mg Zn kg-1 of DM (R4). The initial live weight of sheep was 11.99 ± 0.17kg h-1. Live weight were measured fortnightly and feed consumption were measured daily. The experiment was carried out for 12 weeks with 4 weeks prior adaptation periods. Data obtained were analyzed statistically based on Completely Randomized Design. The results of experiment showed that the supplementation of Zn-bio-complex improved the live weight from 57.60 g h-1d-1 (R1) to 85.47, 72.14 and 67.86 g h-1d-1 with the feed conversion ratio improvement from 11.9 (R1) to 8.0, 9.6 and 10.5 for treatment R2, R3 and R4 respectively (P<0.05). The graded level of Zn-bio-complex did not affect daily feed intake which was 687, 686, 695 and 711 g h-1d-1 (P>0.05) for R1, R2, R3 and R4, respectively. It can be concluded that the supplementation of Zn-bio-complex at the level of 50 mg Zn kg-1 in daily concentrate resulted in the best response on live weight gain and feed conversion ratio of young sheep. Key Words: Zn-Biocomplex, Sheep, Liveweight Gain, Feed Conversion Ratio
Economic Assessment of Fattening Beef Cattle Through The Rice Straw Utilization Atien Priyanti; T Kostaman; B Haryanto; K Diwyanto
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 11, No 1 (2001)
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.903 KB) | DOI: 10.14334/wartazoa.v11i1.759

Abstract

Based on the agricultural production, Indonesia has very high potential to increase rice production through the implementation of adapted and liable technology. The role of non-rice sub sector is also needed to harmonize management in various aspects, in which one of them is livestock sub sector. This management could be done through the development of livestock farming using the rice straw as one of the potential animal feed. The objective of the study was to get a conceptual integrated systems of rice production based on livestock (cattle) development. The estimation of bio-economic for rice straw fermented utilization as animal feed has been done through observation of the cattle biological parameter. An economic approach that has been used in the study was the partial budget analysis including the feed cost per gain (FC/g) and gross margin analysis. The results of the study has shown that FC/g of the cattle from Boyolali and Sumba Ongole (SO) were the most efficient in using the feed compared to the other four cattle. Those were Rp.4600,- and Rp.4995,- per head per day for cattle from Boyolali and SO, respectively. The two types of cattle also reached the gross margin estimation, however SO cattle was slightly less than that of the cattle from Boyolali (Rp.2854,- vs Rp.2897,- ) per head per day. The differences were very much depending on the growth rate of each type of the cattle.   Key words: Feed cost per gain, gross margin estimation, rice straw, beef cattle farming