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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu-Ilmu Pertanian Indonesia Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat TERRA : Journal of Land Restoration Abditani : Jurnal Pengabdian Masyarakat Akta Agrosia Dharma Raflesia : Jurnal Ilmiah Pengembangan dan Penerapan IPTEKS PAKDEMAS : Jurnal Pengabdian Kepada Masyarakat
Reny Herawati
University Of Bengkulu
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Journal : Akta Agrosia

 1 
Induksi Kalus dan Regenerasi Tanaman pada Kultur Antera Persilangan Padi Indica Varietas Lokal Bengkulu Herawati, Reny; Rustikawati, Rustikawati; Inoriyah, Entang
Akta Agrosia Vol 18 No 2 (2015)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Establishment of homozygous lines can be accelerated with anther culture technique that can produce pure lines in one generation. Formation of spontaneous double haploid plants in rice anther culture is very beneficial, because it does not need to be doubled haploid plants as material selection.This study aims to obtain a doubled haploid lines of upland rice is tolerant to drought and blast disease resistance. The main ingredient in this research is anther crosses of rice plants (F1), which consists of local varieties with selected lines of P1 (Sriwijaya x IR-148), P2 (Sriwijaya x IR-7858-1), P3 (Bugis x IR -148), and P4 (Bugis x IR-7858-1), callus induction media (N6), regeneration medium (MS). Anther culture of local varieties of rice indica/indica produced callus induction and plant regeneration are low, resulting in low efficiency of anther culture in providing green plants. The use of donor parents indica/j aponica which is responsive to anther culture into consideration for the material F1 crosses. Anther inoculation needs to be done in several stages so that the opportunity to obtain plantlets in sufficient quantities for material selection will be greater.
Genetic Diversity and Agromorphological Characteristics of 50 Upland Rice Genotypes Derived from Recurrent Selection Yono, Sahri; Herawati, Reny; Pujiwati, Hesti
Akta Agrosia Vol 21 No 2 (2018)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Rice (Oryza sativa L.) is the most important crops commodity in Indonesia because it is the main staple for the people of Indonesia but currently,  in Indonesia, the production of rice is still not maximal so it can not meet the demand of Indonesian people for national food needs. Therefore, it is necessary for rice plants that have high production and tolerant to various stresses. Plant breeding  is one of the ways that can be done to increase the production of rice, one of which is the method of cross-recurring selection. Genetic diversity is a variation of characteristics that are inherited in the same species population. This study aims to obtain information about genetic diversity and agro-morphological characters of 50 genotypes rice derived from the recurrent selection. This research was conducted from August until December 2017 in Rawa Makmur Village, Bengkulu Province with the height of place from sea level ± 2 m. The study used a Completely Randomized Block Design (RAK) with 3 replications. The seeds used were 50 genotypes of rice from previous cross-selection results from Bugis/N-22 crossbreeds, Bugis/IR-7858-1, Sriwijaya/N-148, Sriwijaya/IR-7858-1. The results showed that the genetic diversity of rice is quite narrow to moderate, but there are some rice characters that have medium to high heritability value that was plant height, harvesting age, flowering age, the total number of tillers and panicle length, so that character can be used for the next generation rice selection. The genotypes produced by Bugis/IR-7858-1 crosses have panicle length, filled grain per panicle, and high grain weight/hill resulting in high yield potential.
Induction and Growth of Endosperm Cali of Rimau Gerga Lebong (RGL) Citrus on Several Media Compositions Sari, Rossi Anandayu; Herawati, Reny; Herison, Catur
Akta Agrosia Vol 22 No 2 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Rimau Gerga Lebong (RGL) variety is one of the main orange fruit commodities in Lebong Regency of Bengkulu Province, which has a competitive advantage and has good market potential. However, high number of seed characteristic makes this orange fruit becomes less popular.  Triploid genotype formation through endosperm culture in vitro is an alternative solution to develop seedless orange fruit.  The objective of this study was to determine the best composition medium for callus induction of endosperm of RGL orange seeds as the foremost step of in vitro triploid plant development. The research was conducted from August  2017  until  December  2018 at the Plant  Tissue Culture Laboratory, Department of Agronomy, Faculty of Agriculture, University of Bengkulu.  The  experiment  was  arranged  in  a  completely randomized design, with eight treatment combinations i.e. G1 (MT + 5 ppm BAP + 2 ppm 2,4-D), G2 (MT + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm CH), G3 (MT + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm + 0.5 ppm Kinetin), G4 (MT + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm CH + 0.5 ppm to 500 ppm Kinetin + ME) , G5 (MS + 5 ppm BAP + 2 ppm 2,4-D), G6 (MS + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm CH), G7 (MS + 5 ppm BAP + 2 ppm 2,4- D + 500 ppm CH + 0.5 ppm Kinetin), G8 (MS + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm CH + 0.5 ppm to 500 ppm Kinetin + ME), with three replications. The experimental unit was five culture bottles containing three explants per bottle.  Observations were conducted on days to callus formation, rate of callus formation, callus weight, callus diameter, callus color and texture.  The results showed that media of MS + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm CH was the best media for callus induction, and MT + 5 ppm BAP + 2 ppm 2,4-D + 500 ppm CH was the best media for callus development.Keyword: RGL citrus, calli, endosperm, kinetin, BAP
Acclimatization of Pencil Orchid (Papillionanthe hookeriana Rehb.f) as Affected by Different Types of Planting Media and Fertilizing Frequency Febriyani, Sustri; Ganefianti, Dwi W; Romeida, Atra; Herawati, Reny
Akta Agrosia Vol 22 No 1 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Acclimatization is a process of an environmental adaptation from heterotrophic conditions to autotrophic conditions. In order to be successful in acclimatization, it requires proper planting media and enough nutrition supply. The objective of this experiment were to determine the best planting media and the best spraying frequency of leaf fertilizer on Pencil orchid growth during acclimatization period.  The experimental design used was Randomized Complete Block Design, arranged in factorial (2 factors, 3 replications). The first factor was type of planting media: Coconut Coil, Rockwool, Wood Shavings, and Fern Roots. The second factor tested was fertilizing frequencies: every 2 days, every 3 days, and every 4 days. The results showed that the best media for acclimatization of Pencil orchid was fern root fertilized every four days, the best media for growing Pencil orchid was fern root and Wood Shavings, and the best fertilizing frequency to promote Pencil orchid growth were every 3 days or every 4 days.Keywords: Pencil orchid, acclimatization, planting media, fertilization
Growth and Yield of Lettuce (Lactuca sativa L.) by Giving Cow Manure and Empty Palm Oil Bunch Fertilizer on Peat Soil Manullang, Yedija; Herawati, Reny; Handajaningsih, Merakati; Ganefianti, Dwi Wahyuni; Haquarsum, Eka J.V.; Sutrawati, Mimi
Akta Agrosia Vol 23 No 2 (2020)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Lettuce (Lactuca sativa L.) is a horticultural plant that is beneficial for health. Efforts are being made to expand the plant area. One of them is by utilizing marginal land such as peatlands.  Peatlands in Indonesia are the most extensive soils in various tropical countries in the world that have low soil fertility and high acidity so that they are not optimal for lettuce growth. The provision of organic material can increase soil fertility by improving physical, chemical, and biological soil properties so that it can support lettuce growth. This study aims to determine the dose of cow manure and the dosage of oil palm empty fruit bunch fertilizer and the optimal dose interaction for growth and yield of lettuce. This research was conducted from June 2019 to August 2019 in Bengkulu City. This study uses a Completely Randomized Design consisting of two factors, which are repeated three times. The first factor is the dose of cow manure with three levels, namely: 0 tons ha-1, 15 tons ha-1, and 30 tons ha-1. The second factor is the dose of oil palm empty fruit bunch fertilizer, which consists of four levels, namely 0 tons ha-1, 5 tons ha-1, 10 tons ha-1, and 15 tons ha-1. The results showed that the dosage of cow manure 15 tons ha-1 and without the application of empty fruit bunches fertilizer gave the best results in leaf length of 18.674 cm. The best dose of cow manure for growth and yield of lettuce is 15 ha-1. The application of OPEFB fertilizer has not been shown to affect the growth and yield of lettuce.
Co-Authors Antoni Sitorus Atra Romeida Atra Romeida Bambang S. Purwoko Bambang Sapta Purwoko Barchia, M. Faiz Buang Abdullah David Khairullah Hadi Dwi W Ganefianti Dwi Wahyuni Ganefianti, Dwi Wahyuni Eko Suprijono Febriyani, Sustri Haquarsum, Eka J.V. Helfi Eka Saputra Herison, Catur Heru Widiyono, Heru Heryanto, Valen HESTI PUJIwATI Inoriyah, Entang Iswari S. Dewi Manullang, Yedija Marlin Marlin Merakati Handajaningsih Mimi Sutrawati Muhamad Solihin Mukhtasar Mukhtasar Nurul Khumaida Padang, Agri Andi Saputra Rustikawati Rustikawati Sari, Rossi Anandayu Selvia Wulan Hajijah Simanihuruk, Bilman W. Sumardi Widodo w Widodo Widodo Yono, Sahri