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IDENTIFICATION OF M4 GAMMA IRRADIATED MAIZE MUTANT BASED ON RAPD MARKERS Rustikawati, Rustikawati; Suprijono, Eko; Romeida, Atra; Herison, Catur; Sutjahjo, Surjono H.
AGRIVITA, Journal of Agricultural Science Vol 34, No 2 (2012)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v34i2.102

Abstract

Gamma irradiation to induce mutation in plant has been used intensively since several decades ago.  On maize, 275 Gy gamma irradiation have been known to increase genetic variability indicated by their morphological variation.  Identification on genetic changes by molecular technique is important to answer whether there is mutation happening on DNA level of the plants.   The objective of this research was to identify RAPD marker polymorphism on gamma irradiation mutants compared to their parents.  The initial step of the research was to select random primers could positively amplify the maize DNA.  The result showed that selection on 60 random primers yielded 15 primers that positively amplified the maize DNA.  Amplification on both mutants and their parents by those 15 selected primers indicated that only 5 primers yielding polymorphism between mutants and their parents.  Polymorphisms on mutant G1, G3 and G6 were detected on one locus, meanwhile on mutant G7, G8 and G9 were on two loci.
Rooting of Mini-Cuttings of guava cultivars (Psidium guajava L.) Treated with IBA under Misting Irrigation Supanjani, Supanjani; Rahayu, Hatari; Suprijono, Eko; Romeida, Atra; Yulian, Yulian
Akta Agrosia Vol 23, No 1 (2020)
Publisher : Faculty of Agriculture, The University of Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/aa.23.1.27-32

Abstract

       Guava (Psidium guajava L.) is a plant whose fruit is very popular in Indonesia because it contains high vitamin C and its leaves can be used as herbal medicine. Orchard expansion of selected cultivars and fruit production of guava can be achieved vegetatively through  propagation by using mini-cuttings with the use of Indole Butyric Acids (IBA). A study was conducted to compare rooting success of mini-cuttings of herbaceous stem of four guava cultivars with IBA treatment. Four guava cultivars tested were Bengkulu Round, Getas, Crystal, and Bangkok.  Mini-cuttings were immersed in IBA solution at 0 ppm or 1000 ppm. Cultivar and IBA treatments were arranged factorially in a randomized complete  block design with  3 replications, each consisting of 25 cuttings. Variables observed included percentage of original-leaf shedding, percentage of emerging shoots, percentage of sprouting cuttings, new leaf number, root number, root length, and percent of rooted cuttings. The results demonstrated that cultivars affected rooting percentage, sprouted percentage, percentage of original-leaf fall, and number of root.  Getas had highest rooting and sprouting percentages, and showed lowest number of  original-leaf shedding; whereas, Bangkok demostrated greatest number of root.  IBA treatment increased the number of root and the number of original-leaf shedding, but reducing rooting percentage of mini-cutting. There was no interaction between cultivar and IBA teratment.
Penerapan Budidaya Cabai Dengan Sistem Tanam Kombinasi Pada Kelompok Wanita Tani Anggrek Pematang Gubernur Kota Bengkulu Herawati, Reny; Ganefianti, Dwi Wahyuni; Romeida, Atra
DHARMA RAFLESIA Vol 21 No 1 (2023): JUNI (ACCREDITED SINTA 5)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/dr.v21i1.27704

Abstract

Penggunaan lahan pertanian penduduk sebagian besar digunakan untuk persawahan dan kebun untuk kebutuhan keluarga yang ditanam sayuran seperti cabai tomat, terong, dan lain-lain.  Teknik budidaya cabai yang dilakukan masih sangat tradisional dan didominasi oleh serangan hama dan penyakit sehingga produktivitasnya sangat rendah.  Oleh karena itu perlu dilakukan pembinaan bagaimana mengoptimalkan pemanfaatan lahan pekarangan maupun kebun dengan teknologi budidaya cabai dengan sistem tanam kombinasi.  Kegiatan pengabdian masyarakat dengan topik “Penerapan Budidaya  Cabai dengan Sistem Tanam Kombinasi” dilaksanakan di Kelompok Tani Wanita Anggrek, Kelurahan Pematang Gubernur, pada bulan Agustus-November 2022. Penerapan budidaya cabai diterapkan di kebun masyarakat sebagai damplot KWT Anggrek  Pematang Gubernur. Pelaksanaan kegiatan melibatkan mahasiswa Program Studi Agroekoteknologi sebagai bentuk pembelajaran dalam transfer teknologi kepada masyarakat sebelum mereka terjun langsung ke masyarakat dalam melaksanakan program KKN.  Metode pengabdian dalam bentuk penyuluhan menyampaikan topik materi dan dilanjutkan dengan tanya jawab dan demplot di kebun masyarakat setempat. Penyampaian materi mulai dari pembibitan sampai panen. Kemudian dilanjutkan dengan membuat damplot dan peragaan cara menanam cabai dengan teknologi kombinasi beberapa varietas hibrida. Wawasan dan pengetahuan ibu-ibu KWT Anggrek semakin luas dari kegiatan pengabdian masyarakat tentang budidaya cabai dengan sistem tanam kombinasi, sehingga dapat mengurangi serangan hama dan penyakit, mengurangi penggunaan pestisida.  Hasil panen dapat dimanfaatkan untuk menambah pendapatan keluarga dan mengurangi biaya bumbu dapur untuk kebutuhan sehari-hari, bahkan dari hasilnya dapat menambah income keluarga. Untuk dapat berwirausaha secara mandiri diperlukan arahan secara kontinyu terutama bimbingan dalam mengolah hasil panen dengan berbagai macam olahan yang bernilai ekonomi dan dapat dipasarkan dengan jangkauan lebih luas.
Pemanfaatan Limbah Sayuran dan Buah untuk Pembuatan Ekoenzim di Desa Lawang Agung Kabupaten Seluma, Provinsi Bengkulu Sutrawati, Mimi; Romeida, Atra; Anwar, Guswarni
DHARMA RAFLESIA Vol 22 No 1 (2024): JUNI (ACCREDITED SINTA 5)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/dr.v22i1.35995

Abstract

Limbah buah dapat dimanfaatkan sebagai bahan baku pembuatan ekoenzim. Selama ini limbah buah biasanya hanya dimanfaatkan sebagai bahan baku kompos, padahal banyak cara pemanfaatan limbah yang dapat menghasilkan beragam produk untuk keperluan sehari-hari. Tujuan kegiatan pengabdian ini untuk menyebarkan teknik sederhana pemanfaatan limbah rumah tangga yaitu fermentasi limbah buah menjadi ekoenzim. Kegiatan Pengabdian pada Masyarakat (PPM) ini dilakukan oleh tim Kewirausahaan Fakultas Pertanian Universitas Bengkulu. Kegiatan diawali dengan penjelasan materi tentang ecoenzim, pemanfaatannya dalam kehidupan sehari-hari dan praktek pembuatan ekoenzim skala rumah tangga. Evaluasi menunjukkan peningkatan pengetahuan masyarakat tentang ekoenzim. Peserta umumnya tertarik menerapkan teknologi pembuatan ekoenzim untuk pengolahan limbah buah di rumah masing-masing.
Evaluasi Kesesuaian Lahan Tambak Udang Di Wilayah Pesisir Kabupaten Kaur Saputra, Eko Adi; Barchia, M. Faiz; Utama, Satria Putra; Romeida, Atra; Susatya, Agus
Naturalis: Jurnal Penelitian Pengelolaan Sumberdaya Alam dan Lingkungan Vol. 12 No. 2 (2023)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/naturalis.12.2.30728

Abstract

Penelitian  ini  bertujuan  untuk mengetahui  kesesuaian lahan tambak udang di wilayah pesisir Kabupaten Kaur. Sampel pengamatan sebanyak 21 tambak wilayah pesisir Kabupaten Kaur pada tahun 2019. Parameter pengukuran meliputi aspek tanah (topografi dan jenis tanah), Kualitas dan Sumberdaya Air (suhu air, pH air dan jarak dari pantai) dan Iklim (curah hujan), sedangkan analisis data menggunakan deskriptif kuantitatif. Hasil  penelitian  diketahui bahwa dari 133 ha tambak udang, terdapat 15 tambak (71,42%) yang tidak sesuai  berada di Kecamatan Kaur Tengah (T1, T1), Kecamatan Tetap (T3), Kecamatan Kaur Selatan (T4, T5, T7, T8, T10, T11, T12, T13, T14, T15), Kecamatan Maje (T16), dan Kecamatan Nasal (T21), sedangkan 6 tambak (28,58%) sesuai berada di Kecamatan Kaur Selatan (T6, T9) dan Kecamatan Maje (T17, T18, T19, T20). Ketidaksesuaian lahan tambak ini karena bertentangan dengan Peraturan Presiden Nomor 51 Tahun 2016 Tentang Batas Sempadan Pantai dan Perda No.04 Tahun 2012 tentang Rencana Tata Ruang Wilayah Kabupaten Kaur Tahun 2012-2032, Pasal 21 angka (2) disebutkan bahwa kawasan sempadan pantai berupa kawasan daratan sepanjang tepian laut dengan jarak paling sedikit 100 (seratus) meter dari titik pasang air laut tertinggi ke arah daratan. Dengan demikian 15 tambak berada di sepadan pantai dan 6 tambak di luar sepadan pantai.
Potensi Produksi Enam Hibrida Jagung pada Ultisol di Provinsi Bengkulu Suprijono, Eko; Rustikawati, Rustikawati; Romeida, Atra; Gustian, Meko
Akta Agrosia Vol 18 No 2 (2015)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4289.503 KB)

Abstract

Ultisol is one of marginal land type quite extensively presence in Indonesia, including the province of Bengkulu. The use of varieties tolerance to acidity stress is the best manner to utilize of this type of land with relatively low cost and environmentally friendly. The objective of this research was to evaluate theyield potential of six maize hybrids developed to soil acidity tolerance. The experiment was conducted at the Experimental Farm Medan Baru, Kandang Limun Village, Muara Bangkahulu District, Bengkulu City, from August to November 2015. The experiment was arranged in a randomized completely block design with four replications. As the treatment were newly developed maize hybrids, namely CT5, CT8, CT9, CT13, CT14 and CT15. The variables measured were cob length, cob diameter, 100-seed weight and the estimated yield/ha. Qualitative variables supporting to corn yield were the cob position, seed color, seed type and seed row arrangement. The results indicated that the hybrid CT8 showed the highest estimated yield in Ultisol among hybrids evaluated, with the estimation of 6.5 tons per hectare. Qualitatively, theseeds of hybrid CT8 were a horse tooth type, pale yellow color, and stright line arrangement.
Acclimatization of Pencil Orchid (Papillionanthe hookeriana Rehb.f) as Affected by Different Types of Planting Media and Fertilizing Frequency Febriyani, Sustri; Ganefianti, Dwi W; Romeida, Atra; Herawati, Reny
Akta Agrosia Vol 22 No 1 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1098.752 KB)

Abstract

Acclimatization is a process of an environmental adaptation from heterotrophic conditions to autotrophic conditions. In order to be successful in acclimatization, it requires proper planting media and enough nutrition supply. The objective of this experiment were to determine the best planting media and the best spraying frequency of leaf fertilizer on Pencil orchid growth during acclimatization period.  The experimental design used was Randomized Complete Block Design, arranged in factorial (2 factors, 3 replications). The first factor was type of planting media: Coconut Coil, Rockwool, Wood Shavings, and Fern Roots. The second factor tested was fertilizing frequencies: every 2 days, every 3 days, and every 4 days. The results showed that the best media for acclimatization of Pencil orchid was fern root fertilized every four days, the best media for growing Pencil orchid was fern root and Wood Shavings, and the best fertilizing frequency to promote Pencil orchid growth were every 3 days or every 4 days.Keywords: Pencil orchid, acclimatization, planting media, fertilization
Bio-fortified Compost as A Substitute for Chemical N Fertilizer for Growth, N Accumulation, and Yield of Sweet Corn Marwanto, Marwanto; Wati, Shinta Puspita; Romeida, Atra; Handajaningsih, Merakati; Adiprasetyo, Teguh; Hidayat, Hidayat; Purnomo, Bambang; Murcitro, Bambang Gonggo; Siswanto, Usman
Akta Agrosia Vol 22 No 2 (2019)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRACTFinding the appropriate method of fertilizer application to simultaneously enhance farm productivity and ensure ecosystem sustainability has been receiving a lot of attention. A field experiment was carried in the Research Plot Agriculture Faculty Bengkulu University Campus Indonesia in 2017. The purpose of this study was (1) to assess the significant effects of inorganic N fertilizer (IF) substitution with bio-fortified compost (BC) under equal N conditions on growth, N accumulation, and yield of sweet corn, and (2) to determine the appropriate level to which inorganic N fertilizer could be reduced and equivalently replaced by bio-fortified compost to promote sweet corn growth and yield. The treatments consisted of six different proportions of inorganic N fertilizer (IF) substitution with bio-fortified compost (BC). Each treatment was designed on the basis of equal amount of total N input from a combination of both fertilizers (138 kg N ha-1).  They were arranged in a randomized block design with 3 replications. They included (1) 100% IF plus 0% BC, (2) 75% IF plus 25% BC, (3) 50% IF plus 50% BC, (4) 25% IF plus 75%, (5) 0% IF plus 100% BC, and (6) no IF and no BC. The results showed that the increasing proportion of IF replaced by BC resulted in an increase for all variables (except for plant height) but they decreased when 100% IF substitution with 100% BC was applied. Among the partial substitution treatments, 50% IF plus 50% BC consistently produced the best growth, N accumulation, and yield increase. Treatments of 50% IF plus 50% BC and 25% IF plus 75% BC produced the highest green cob weight with husk per plot (10.74 – 10.84 kg plot-1), which was 16% to 19% higher than treatment of 100% IF plus % BC. The three partial substitution treatments produced crop yield components as good as treatment of 100% IF plus 0% BC. Treatment of 0% IF plus 100% BC reduced plant growth, N accumulation, and crop yield and its components. The appropriate level to which IF could be reduced and equivalently replaced by BC was at the range of 25% to 75%. Hence, a suitable replacement of inorganic N fertilizer with bio-fortified compost is considered a reasoned way to simultaneously increase crop yield and reduce environmental degradation.Keywords: organic fertilizer, chemical fertilizer, bio-fortification, chemical fertilizer substitution, bio-fortified compost
Rooting of Mini-Cuttings of guava cultivars (Psidium guajava L.) Treated with IBA under Misting Irrigation Supanjani, Supanjani; Rahayu, Hatari; Suprijono, Eko; Romeida, Atra; Yulian, Yulian
Akta Agrosia Vol 23 No 1 (2020)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

       Guava (Psidium guajava L.) is a plant whose fruit is very popular in Indonesia because it contains high vitamin C and its leaves can be used as herbal medicine. Orchard expansion of selected cultivars and fruit production of guava can be achieved vegetatively through  propagation by using mini-cuttings with the use of Indole Butyric Acids (IBA). A study was conducted to compare rooting success of mini-cuttings of herbaceous stem of four guava cultivars with IBA treatment. Four guava cultivars tested were Bengkulu Round, Getas, Crystal, and Bangkok.  Mini-cuttings were immersed in IBA solution at 0 ppm or 1000 ppm. Cultivar and IBA treatments were arranged factorially in a randomized complete  block design with  3 replications, each consisting of 25 cuttings. Variables observed included percentage of original-leaf shedding, percentage of emerging shoots, percentage of sprouting cuttings, new leaf number, root number, root length, and percent of rooted cuttings. The results demonstrated that cultivars affected rooting percentage, sprouted percentage, percentage of original-leaf fall, and number of root.  Getas had highest rooting and sprouting percentages, and showed lowest number of  original-leaf shedding; whereas, Bangkok demostrated greatest number of root.  IBA treatment increased the number of root and the number of original-leaf shedding, but reducing rooting percentage of mini-cutting. There was no interaction between cultivar and IBA teratment.
Kajian Pengelolaan Limbah Bahan Berbahaya Dan Beracun (Lb3) Padat Yang Dihasilkan Puskesmas Di Kabupaten Lebong Rani, Eka; Utama, Satria Putra; Uker, Damres; Martono, Agus; Romeida, Atra
Naturalis: Jurnal Penelitian Pengelolaan Sumberdaya Alam dan Lingkungan Vol. 13 No. 2 (2024)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/naturalis.13.2.37133

Abstract

Health centers in Lebong Regency have the potential to produce solid waste of the B3 type, so it is necessary to pay attention to its management starting from the source, generation, storage, collection, and processing of solid B3 waste so as not to have an impact on environmental pollution. Therefore, this study aims to determine the implementation of solid B3 waste management and waste generation and solid B3 waste management facilities of health centers in Lebong Regency. The research method used is a qualitative approach with quantitative data and a descriptive case study design. The study was conducted at 13 health centers in Lebong Regency consisting of 2 health center treatment units and 11 non-treatment health centers. Data collection was carried out by distributing questionnaires to all health centers, interviews, observations and document reviews. The collected data were analyzed descriptively by reviewing, tabulating data, combining research results to answer research questions and assessing their compliance with applicable laws and regulations. Solid B3 waste produced by health centers in Lebong Regency with the characteristics of infectious waste, sharp waste and pharmaceutical waste. The implementation of solid B3 waste management at health centers in Lebong Regency has not fully complied with the Minister of Environment and Forestry Regulation Number P.56/MenLHK-Setjen/2015. Since 2019, the largest solid B3 waste generated by health centers with treatment and non-treatment is sharps waste. Temporary storage facilities for B3 waste that have met the standards are Muara Aman health center, Talang Leak health center and Limau Pit health center. B3 waste management at health centers in Lebong Regency needs to be improved, especially in sorting, storage, provision of cold storage facilities, improvement of solid B3 waste management facilities, improvement of human resource capacity, improvement of SOP quality and budget planning.