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Journal : Makara Journal of Science

A Morphological and Molecular Study of Phallus multicolor in Indonesia Hermawan, Rudy; Putra, Ivan Permana; Amelya, Mega Putri; Gunawan, Mochammad Rizky Wangsadireja
Makara Journal of Science Vol. 26, No. 2
Publisher : UI Scholars Hub

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Abstract

Phallus is known as stinkhorn mushroom. Previous reports have shown that this genus is recognized by the size and color of the basidioma, which is a highly plastic morphological character that can potentially lead to misidentification. In Indonesia, no study has combined morphological and molecular analyses to identify Phallus. This study aimed to determine the identity of Phallus that has the orange color of an indusium found in Bekasi, West Java, Indonesia. The morphological characteristics were described by observing the macroscopic and microscopic features of fresh specimens. Molecular analyses were performed based on the internal transcribed spacer 4/5 region sequence. The results showed that specimen BO24430 was identified as Phallus multicolor with 100% similarity in the BLAST results and a 98% bootstrap value on the phylogenetic tree. This taxonomic placement was supported by morphological features, such as a campanulate pileus, yellowish to white pseudostipe, brown to whitish eggs, and ellipsoid spores. This study is the only record of P. multicolor in Indonesia with a herbarium voucher since Boedijn in 1932. In addition, the current study assessed the diversity of Phallus in Indonesia using morphological and molecular evidence.
Newly Recorded Panus lecomtei (Fr.) Corner (Basidiomycota: Panaceae) for Indonesia Putra, Ivan Permana; Hermawan, Rudy; Salman, Ali Bin Abithalib
Makara Journal of Science Vol. 27, No. 3
Publisher : UI Scholars Hub

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Abstract

Panus lecomtei (Polyporales) is reported for the first time from Indonesia at the IPB University Campus Forest (IPBUCF). This species has similar macroscopic features to P. strigellus and P. neostrigosus. This study aimed to confirm the taxonomic position of P. lecomtei found at IPBUCF based on morphological and molecular evidence. The specimens were described by their macroscopic and microscopic characteristics. A phylogenetic approach was used with the internal transcribed spacer 1/2 sequence data. The combination of morphological and molecular analyses confirmed the taxonomic position of our specimens as P. lecomtei. The recorded species was distinguished by gregarious basidiomata, an infundibuliform resembling a funnel, violet during the immature stage to orangish brown in the mature pileus and ellipsoid basidiospore stages. Our specimens were distinguished from P. strigellus by having smaller basidiospores. It was difficult to separate P. lecomtei BO24428 from P. neostrigosus based on morphological characters. However, the BLAST results showed that specimen BO24428 had 99.56% similarity with P. lecomtei. In addition, our phylogenetic tree nested P. lecomtei BO24428 in the P. lecomtei clade. Morphological illustrations and a phylogenetic tree of P. lecomtei are provided. We suggest combining the morphological and molecular evidence in future studies of P. lecomtei from Indonesia