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All Journal Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Mutiara Medika: Jurnal Kedokteran dan Kesehatan MNJ (Malang Neurology Journal) Medical Laboratory Technology Journal Jurnal Ilmiah Farmako Bahari Jurnal Kebidanan Akademi Kebidanan Jember (JKAKJ) Indonesian Archives of Biomedical Research (InABR) Journal of Fisheries & Marine
Dwi Yuni Nur Hidayati
Program Studi Biomedik, Fakultas Kedokteran, Universitas Brawijaya, Malang
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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Comparison of Methanolic Extract of Piper Betle to Amikacin against the Growth of Pseudomonas aeruginosa Azalia Ayu Rysaputri; Hery Susilo; Dwi Yuni Nur Hidayati; Irene Ratridewi
Jurnal Kedokteran Brawijaya Vol. 32 No. 3 (2023)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2023.032.03.3

Abstract

Pseudomonas aeruginosa is one of the most common pathogens that cause Healthcare-Associated Infections (HAIs). A previous study stated that Piper betle L. extract has antibacterial activity against certain bacteria, including Pseudomonas aeruginosa. This study has the objective of comparing antibacterial activity of the methanolic extract of Piper betle L. and amikacin on the growth of Pseudomonas aeruginosa. This study used the tube dilution method with a sample of Pseudomonas aeruginosa from Microbiology Laboratory Dr. Saiful Anwar General Hospital, Malang. The results of this study showed that the value of MIC and MBC from the methanolic extract of the Piper betle L. treatment was 4800μg/mL. Meanwhile, the amikacin treatment resulted in 0.4μg/mL for MIC and 0.45μg/mL for MBC. From linear regression, it was found that the methanolic extract of Piper betle L. had a β-coefficient value closer to -1 compared to amikacin. Therefore, it can be concluded that the methanolic extract of Piper betle L. has a greater inhibiting effect on the growth of Pseudomonas aeruginosa.
Identifikasi Molekul Adhesi Pili Pseudomonas aeruginosa pada Human Umbilical Vein Endothelial Cells (HUVECs) Culture Dwi Yuni Nur Hidayati
The Journal of Experimental Life Science Vol. 1 No. 1 (2011)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (824.212 KB) | DOI: 10.21776/ub.jels.2011.001.01.02

Abstract

Pseudomonas aeruginosa merupakan salah satu penyebab Gram negatif bakteriemia yang bisa berlanjut menjadi sepsis. Bakteri ini banyak menginfeksi penderita di rumah sakit dengan membentuk koloni pada pembuluh darah melalui proses adhesi (pelekatan). Pili dan bagian Outer Membrane Protein (OMP) adalah faktor yang mempengaruhi pelekatan bakteri P. aeruginosa. Tujuan dari penelitian ini adalah untuk mengetahui berat molekul protein hemaglutinin yang terdapat pada pili dan membuktikan peran protein hemaglutinin pada pili dalam pelekatan bakteri P. aeruginosa. Metode yang digunakan dalam penelitian ini adalah isolasi protein pili P. aeruginosa secara bertingkat, dilanjutkan dengan uji hemaglutinasi (metode mikrotiter) dan uji adhesi menggunakan protein pili hasil elektroelusi yang disalutkan pada kultur sel endotel (HUVECs) (konsentrasi 1, ½, ¼, ⅛, ⅟16, dan 0 [kontrol]). Hasil penelitian menunjukkan adanya reaksi hemaglutinasi pada potongan pili ketiga dengan titer tertinggi (1/128). Protein hemaglutinin dengan berat molekul 38,19 kDa memberikan titer tertinggi (⅟16). Hasil uji adhesi protein hemaglutinin yang disalutkan pada sel endotel menunjukkan bahwa semakin tinggi pengenceran maka adhesi bakteri menunjukkan peningkatan secara signifikan dengan konstanta regresi (r)= 0,98 dan p value= 0,00. Berdasarkan penelitian dapat disimpulkan bahwa protein hemaglutinin pili merupakan protein adhesin. Protein adhesin dengan berat molekul 38,19 kDa berperan pada perlekatan bakteri P. aeruginosa 9064 dengan kultur sel endotel (HUVECs).   Kata kunci: adhesi, hemaglutinasi, pili, Pseudomonas  aeruginosa
Pengaruh Induksi Bakteri Pseudomonas aeruginosa Terhadap Human Umbilical Vein Endothelial Cells (HUVECS) Culture [ The Influence Of Pseudomonas aeruginosa Induction To The Human Umbilical Vein Endothelial Cells (HUVECS) Culture ] Dwi Yuni Nur Hidayati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 1 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i1.11691

Abstract

Abstract One of the cause of negative gram bacteria is Pseudomonas aeruginosa (Ps. aeruginosa). It continuing become sepsis on the first step of patogenesa happen sticking between aeruginosa with endhotel cell. This sticking is mediated by adhesi molecul which have characterize same with hemaglutin protein. The main study of this research is to know the profile of endothelial cell culture within induction of Ps. aeruginosa. The methods are Ps. aeruginosa (9064) which have been isolated then processes on TCBS Media and continued with isolation terracely. The profile of weight molecule protein obtained by SDS PAGE and then conducted electroelution. The test of hemaglutinin using eritrosit mencit. The receptor cell used is endhotel cell (HUVECs) culture. The result show that dosage of hemaglutin protein of Aeruginosa bring effect to profile of HUVECs culture determined by index adhesion of Ps. aeruginosa to HUVECs culture. We conclude that protein 38,19 kDa give effect to profile of HUVECs culture in vitro.
Co-Authors Agustina Tri Endharti Andre William Tulle Aris Widayati Azalia Ayu Rysaputri Elisa Danik Kurniawati Eviana Norahmawati Hartono, Kevin Hening Ryan Aryani Hery Susilo Hidayat Sujuti Irene Ratridewi Istifadatul Ilmiya Ni Putu Sri Haryati Noorhamdani A.S. Noorhamdani Noorhamdani Nuning Winaris Nur Rohmah Prihantanti Nur Rohmah Prihatanti Prasetyo Adi Rima Nor Kaspia, Rima Nor Roekistiningsih Roekistiningsih Roekistiningsih Roekistiningsih Santoso, Giovani Anggasta Soeyati Poejiani Sri Andarini Sri Murwani Tanti Tri Lestary Tatit Nurseta Wening Prastowo Wiyasa, I Wayan Arsana