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Salomo Hutahaean

Departemen Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara.

Author-ID : 283790

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Public Health

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Synthetic Peptides as Immunogen in the Production of IgY Anti-C-Myc Antibody in Local Chicken (Gallus Gallus) Salomo Hutahaean
International Journal of Ecophysiology Vol. 1 No. 1 (2019): International Journal of Ecophysiology
Publisher : Talenta Publisher

Experiments conducted to determine the effect of doses of immunogen on the levels of IgY c-Myc antibody in chickens. To generate antibody, we used a synthetic peptide as an immunogen, which is developed from the epitope of the c-Myc protein. Hens were immunized a week after their first eggs were produced. Antigen diluted in double distilled water and emulsified with Freund 's Complete Adjuvant. The suspension was injected into the area of the chicken breast subcutaneously with a dose of 0, 0.02, 0.04, 0.06, 0.08, 0.1, 0.12, 0.14, or 0.16 mg per hen. Booster injection performed on days 10, 20, and 30 with the same volume of emulsion, but using Incomplete Freund 's Adjuvant and the amount of antigen was half of the amount of the first Immunization. Extraction of IgY from eggs was carried out using kits from Gallus Immunotech. The level of IgY was quantified using a spectrophotometer where the absorbance was read at 280 nm. The results showed that after the first immunization IgY content of the egg were reached the level of >5 mg/ml yolk when the dose of immunogen was above 0.1 mg/hen. The level was higher in eggs produced after the third booster ( >8 mg/ml yolk), but it is lower in all groups treated with immunogen above 0.1 mg/hen. We concluded, immunogen dosage of 0.1 mg/hen was optimum in the production of chicken IgY anti-c-Myc.

Synthetic Peptides as Immunogen in the Production of IgY Anti-C-Myc Antibody in Local Chicken (Gallus gallus) Salomo Hutahaean
International Journal of Ecophysiology Vol. 1 No. 2 (2019): International Journal of Ecophysiology
Publisher : Talenta Publisher

Experiments conducted to determine the effect of doses of immunogen on the levels of IgY c-Myc antibody in chickens. To generate antibody, we used a synthetic peptide as an immunogen, which is developed from the epitope of the c-Myc protein. Hens were immunized a week after their first eggs were produced. Antigen diluted in double distilled water and emulsified with Freund 's Complete Adjuvant. The suspension was injected into the area of the chicken breast subcutaneously with a dose of 0, 0.02, 0.04, 0.06, 0.08, 0.1, 0.12, 0.14, or 0.16 mg per hen. Booster injection performed on days 10, 20, and 30 with the same volume of emulsion, but using Incomplete Freund 's Adjuvant and the amount of antigen was half of the amount of the first Immunization. Extraction of IgY from eggs was carried out using kits from Gallus Immunotech. The level of IgY was quantified using a spectrophotometer where the absorbance was read at 280 nm. The results showed that after the first immunization IgY content of the egg were reached the level of >5 mg/ml yolk when the dose of immunogen was above 0.1 mg/hen. The level was higher in eggs produced after the third booster ( >8 mg/ml yolk), but it is lower in all groups treated with immunogen above 0.1 mg/hen. We concluded, immunogen dosage of 0.1 mg/hen was optimum in the production of chicken IgY anti-c-Myc.

UJI EFEK NEFROPROTEKTIF EKSTRAK ETANOL DAUN BIWA (Eriobotrya japonica Lindl) TERHADAP KADAR KREATININ TIKUS MODEL KANKER PAYUDARA YANG DIINDUKSI Benzo(a)Pyrene Syahfitri, Widya; Elizabeth, Fitri; Putri, Syarifah Riska Mela; Maelani, Imelda; Hutahaean, Salomo; Ilyas, Syarifuddin
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.11120

Benzo(a)pyrene (BaP) merupakan senyawa toksik dan karsinogenik yang dapat menyebabkan stres oksidatif, peradangan, dan gangguan fungsi organ, termasuk ginjal. Penelitian ini bertujuan untuk menguji efek nefroprotektif ekstrak metanol daun biwa (Eriobotrya japonica) terhadap kadar kreatinin tikus model kanker payudara yang diinduksi BaP. Penelitian ini menggunakan rancangan acak lengkap dengan lima kelompok perlakuan, yaitu kontrol negatif (tanpa perlakuan), kontrol positif (BaP 50 mg/kg BB), serta tiga kelompok perlakuan dengan pemberian ekstrak daun biwa dosis 200, 300, dan 400 mg/kg BB selama 30 hari. Kadar kreatinin diukur menggunakan metode modifikasi Jaffe. Data hasil penelitian dianalisis menggunakan uji ANOVA dan dilanjutkan dengan uji Duncan. Hasil penelitian menunjukkan bahwa kadar kreatinin serum darah tikus pada semua kelompok masih berada dalam rentang normal, dengan nilai antara 0,66 hingga 0,73 mg/dl, dan tidak terdapat perbedaan yang signifikan (p0,05) antara kelompok perlakuan dan kontrol. Hal ini mengindikasikan bahwa paparan BaP tidak secara langsung mengganggu fungsi ginjal tikus model kanker payudara, serta pemberian ekstrak daun biwa tidak menunjukkan efek protektif yang signifikan terhadap kadar kreatinin. Namun, kandungan antioksidan dalam daun biwa, seperti flavonoid dan triterpenoid, tetap berpotensi dalam menjaga stabilitas fungsi ginjal melalui mekanisme antioksidatif dan antiinflamasi

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