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All Journal The Indonesian Journal of Dental Research Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Majalah Obat Tradisional Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Odonto dental journal
Suryono Suryono
Department Of Periodontics, Faculty Of Dentistry, Universitas Gadjah Mada
Dentistry Health Professions Medicine & Pharmacology Public Health

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Expression of MRP8/MRP14 mRNA in Monocytes of Periodontitis: Comparison between Diabetic and Non Diabetic Patients Ahmad Syaify; Sri Budi Barunawati; Suryono Suryono; Marsetyawan HNES
The Indonesian Journal of Dental Research Vol 1, No 1 (2010)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (917.236 KB) | DOI: 10.22146/theindjdentres.9986

Abstract

The severity of periodontitis on patients with type 2 Diabetes Mellitus patients was strongly thought caused by decreasing of leukocytes function such as monoctyes and neutrophils. In our previous research it was found that calprotectin (MRP8/MRP14) level in leukocytes of periodontitis patients with type 2 DM was higher than periodontitis in non DM. The aim of this study was to determine calprotectin (MRP8/MRP14)mRNA expression in human monocytes of periodontitis patients with type 2 DM and without DM. Monocytes were isolated from the peripheral blood of periodontitis patients with uncontrolled type 2 DM, controlled type 2 DM, and non DM. The expression of total RNA calprotectin (MRP8 and MRP14) were detected by RTPCR using GAPDH as the innate control. It was observed that the value of MRP8/MRP14 mRNA expression DM patients were higher than non DM, and the highly significant increase expression (p<0.05) was on the uncontrolled type 2 DM. The basal level of MRP8/MRP14 expression increased in monocyte of periodontitis and type 2 DM patients compared with non diabetes subjects. It was suggested that high basal level MRP8/MRP14 has role in the regulation of severity periodontitis with diabetes mellitus.
Pengaruh Cara Aplikasi Pasta Gigi Herbal terhadap Produksi Volatile Sulfur Compound S. Suryono
Majalah Kedokteran Gigi Indonesia Vol 1, No 2 (2015): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (221.565 KB) | DOI: 10.22146/majkedgiind.9122

Abstract

Effect of Herbal Toothpaste Application Method on Production of Volatile Sulfur Compound. Volatile sulfur compound (VSC) is a component of the gas produced by bacteria in the oral cavity, consisting of hydrogen sulfide,methyl mercaptan and dimethyl sulfide. This gas is often used as an indicator to assess the status of bad breath. Herbalbased dental paste has been reported in previous studies to inhibit the growth of germs that cause periodontal disease. Plaque Bacteria in the oral cavity can be reduced by tooth brushing and/or the use of antiseptic material. Two groups of subjects chosen were given treatment using herbal toothpaste in different ways: the first group was by brushing teeth with herbal toothpaste the way they usually do it and the second group was given the treatment by applying herbal paste in the left and right molar region of maxilla. The sampling was performed before treatment, 5 minutes and 2 hours after brushing teeth or applying herbal paste. The result of the experiment indicates that the use of herbal toothpaste with toothbrush and applying method has the same pattern on each component of the sulfur gas that is an increase after five minutes post-application and then a decrease after 2 hours post-application. Herbal toothpaste which is applied by means of brushing or by applying to the teeth affected the levels of VSC components; the level of Hydrogen sulfide decreased, the level of methyl mercaptan was relatively stable, and the level of dimethyl sulfide increased. Herbal toothpaste can be used in a common brushing way or by applying it to the teeth to reduce the levels of VSC gases, especially Hydrogen sulfide in normal subjects.
Incision Below The Clamp sebagai Modifikasi Teknik Insisi pada Frenektomi untuk Minimalisasi Perdarahan S. Suryono
Majalah Kedokteran Gigi Indonesia Vol 18, No 2 (2011): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3125.753 KB) | DOI: 10.22146/majkedgiind.15422

Abstract

Latar Belakang. Perlekatan frenulum tinggi berdampak merugikan bagi jaringan periodontal maupun fungsi estetik. Perlekatan tinggi yang terjadi pada labialis superior, menimbulkan gingivitis dan sentral diastema yang menjadikan indikasi untuk dilakukan frenektomi. Frenektomi dengan teknik melakukan insisi di atas dan di bawah clamp berakibat lebarnya luka karena tarikan otot bibir, yang berdampak terjadi banyak perdarahan. Tujuan. Melakukan evaluasi terhadap modifikasi teknik insisi yang diharapkan dapat mengurangi perdarahan. Kasus dan Penanganan. Laporan kasus ini memaparkan penanganan kasus pada perlekatan frenulum tinggi yang diikuti adanya sentral diastema. Pasien dengan diagnosa frenulum tinggi dan indikasi frenektomi dilakukan tindakan preoperasi. Tindakan operasi dilakukan dengan menggunakan pisau bedah, modifikasi insisi (Insision below the Clamp) dilakukan dengan cara menempatkan clamp frenulum pada posisi yang berdekatan dan sejajar dengan bibir, tindakan insisi dilakukan di bawah clamp, disusul dengan penjahitan pada area mucolabial fold. Selama operasi dilakukan terlihat luka tidak melebar, tidak banyak darah yang keluar, pasien dan dokter merasa nyaman. Kesimpulan. Insision below the Clamp merupakan modifikasi teknik insisi pada frenektomi yang bisa dilakukan oleh para praktisi untuk meminimalisir perdarahan yang terjadi selama proses pengambilan frenulum dengan menggunakan pisau bedah. Background. Attachment of high frenulum has negative impact on periodontal tissues as well as an aesthetic function. High attachment of frenulum that occurs in labialis superior, causing gingivitis and central diastema that are indications to do Frenectomy. Conventional techniques of Frenectomy performed with insisions above and below the clamp resulted in wide injury due to muscle contraction, which affects a lot of bleeding. Objective. To evaluate modification insision technique which hopefully can minimize the bleeding. Case and Treatment. This case report describes the handling of cases at high frenulum attachment that followed the central diastema. Patients with a diagnosis of high frenulum attachment and frenectomy indications be taken preoperatively, Surgery performed by using a scalpel, modification insision (Insision below the Clamp/IBC) is done by placing the clamp frenulum in a position adjacent and parallel to the lip, the insision carried out under the clamp, followed by suturing at the mucolabial fold. During the operation carried no visible wound widened, little of bleeding, patients and physicians are comfortable. Conclusion. IBC is a modification on frenectomy insision technique that can be done by general practitioners to minimize bleeding that occurs during frenectomy by using a scalpel. 
Penggunaan Composit Resin pada Kasus Resisi Gingiva S. Suryono
Majalah Kedokteran Gigi Indonesia Vol 19, No 1 (2012): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (898.817 KB) | DOI: 10.22146/majkedgiind.15921

Abstract

Background: The clinical appearance of gingival tissue play an essential role in aesthetics. Gingival morphology and color effect on the aesthetic concerns for the patient. Gingival recession can cause exposure of the underlying rootsurface and hypersensitive of the tooth. Purpose: this case reports showed the treatment of gingival recession by using gingival-shaded composite. Case and treatment: Exposed root surface is layered by gingival-shaded composite and its also improved aesthetics by replacement of the restoration. Conclusion: The use of gingival-shaded composite in the area of exposed root surface for layering improved the aesthetic and relief the sensitive denting of patient.
Ekspresi Gen CYP19 Aromatase, Estrogen, Androgen pada penderita Periodontitis Agresif Dahlia Herawati; Sri Kadarsih; Wayan T Artama; S. Suryono
Majalah Kedokteran Gigi Indonesia Vol 17, No 1 (2010): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2298.773 KB) | DOI: 10.22146/majkedgiind.15973

Abstract

Kepadatan tulang tubuh ditentukan oleh gen CYP19 aromatase, hormon estrogen dan androgen. Pada periodontitis agresif terjadi perkembangan cepat kerusakan tulang alveolar, dan kerusakan tulang alveoler tersebut tidak diimbangioleh regenerasi tulang. Tujuan penelitian ini adalah menunjukkan ekspresi gen CYP19 aromatase, estrogen, androgen pada penderita periodontitis agresif agar dapat untuk menjadi pertimbangan pada saat melakukan perawatan periodontal. Metode penelitian, pemeriksaan ekspresi gen aromatse CYP19 berasal dari spesimen tulang alveolar menggunakan imunohistokimia, pengukuran hormon estrogen dan androgen dari serum menggunakan Vidas: Elfa. Hasil penelitian ekspresi gene CYP19 aromatase pada periodontitis agresif menunjukkan gambaran lebih rendah densitasnya dibandingkan pada nonperiodontitis. Estrogen dan androgen pad aperiodontitis agresif ada kecenderungan lebih rendah dibandingkan pada nonperiodontitis. Kesimpulan regenerasi tulang alveoler pad a periodontitis agresif terhambat karena sedikitnya gen CYP19 aromatase dan hormon estrogen dan androgen yang berperan pada pembentukan tulang alveoler kurang memadai.
Ekspresi Gen CYP19 dalam Kultur Sel Osteoblas dari Periodontitis Tulang Alveolar Agresif Distimulasi dengan Vitamin D dan atau Deksametason Dahlia Herawati; Sri Kadarsih Soejono; Wayan T Artama; S. Suryono
Majalah Kedokteran Gigi Indonesia Vol 18, No 1 (2011): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6007.659 KB) | DOI: 10.22146/majkedgiind.16444

Abstract

Latar Belakang. Estrogen mengatur homeostasis tulang dan disekresikan oleh gonad dan ekstragonad. Selain itu,androgen diubah menjadiestrogen oleh enzim aromatase P450 yang dihasilkan oleh sitokrom P450 aromatase. Ini diproduksi oleh gen sitokrom CYP19. Vitamin D berperan dalam mengatur homeostasis kalsium dan ekspresi gen aromatase langsung. Deksametason bertindak sebagai anti inflamasi, menghentikan peradangan dan meningkatkan kecepatan penyem6uhan. Kerusakan parah tulang alveolar di periodontitis agresif dapat terjadi di usia muda. Tujuan dari penelitian ini adalah untuk menguji ekspresi gen CYP19daiam kultur sel osteoblas periodontitis agresif tulang alveolar pasien dengan stimulasi vitamin D dan atau deksametason. Metode. Fragmen tulang alveolar dari pasien periodontitis agresif dikultur dalam dimodifikasi F-12 medium dilengkapi dengan serum janin sapi (FBS) 20%, antibiotik (penisilin streptomisin) 5%, dan fungizone 2%. Sel-sel osteoblas yang tumbuh dalam kultur dibagi menjadi 4 kelompok. Kelompok 1: kultur diperlakukan non, kelompok 2: diperlakukan dengan vitamin D 10-6 moVL,kelompok 3: diperlakukan dengan deksametason 10.7mol/L, dan kelompok 4: diperlakukan dengan kombinasi vitamin D den deksametason pada dosis yang sarna. Setelah 24 jam perlakuan dihentikan, semua kelompok kultur yang diamati dengan teknik imunositokimia dan menghitung persentase CYP19 ekspresi gen dan sel oeteoblas dalam kultur. Hasilnya menunjukkan bahwa persentase rata-rata CYP19 ekspresi gen untuk kelompok 1, 2, 3, dan 4 adalah 44.18, 38.66,35.26 dan 31.13%, masing-masing, dan perbedaan yang signifikan antara perlakuan dengan p<0.05. Disimpulkan bahwa perlakuan terbaik untuk meningkatkan jumlah sel osteoblas, adalh kombinasi vitamin D dan deksametason, walaupun persentase ekspresi gen CYP19 adalah nilai terendah di antara kelompok.
Perbedaan Kadar Calprotectin Sebelum Dan Sesudah Radioterapi Pada Pasien Karsinoma Nasofaring Akibat Infeksi Epstein-Barr Virus Rurie Ratna Shantiningsih; S. Suryono
Majalah Kedokteran Gigi Indonesia Vol 18, No 1 (2011): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5591.608 KB) | DOI: 10.22146/majkedgiind.16451

Abstract

Latar belakang: Epstein-Barr Virus (EBV) adalah anggota herpes virus berkaitan dengan etiologi karsinoma nasofaring (KNF). Pada pasien KNF jumlah monosit dalam sel darah tepi mengalami penurunan dan kebanyakan masih dalam bentuk immature sehingga menurunkan respon imun pasien serta meningkatkan kemungkinan terjadinya penyakit periodontal. Radioterapi merupakan salah satu metode terapi yang banyak digunakan untuk kasus KNF. Calprotectin diproduksi dalam sitoplasma sel monosit dan levelnya meningkat pada beberapa penyakit inflamasi, termasuk inflamasi jaringan periodontal, ditandai dengan peningkatan kadar calprotectin pada cairan sulkus gingiva (CSG). Tujuan: mengkaji perbedaan kadar calprotectin pada pasien KNF sebelum dan setelah dilakukan radioterapi, pada sel monosit, serum dan CSG. Metode Penelitian: sepuluh pasien KNF akibat infeksi EBV digunakan sebagai subjek dalam penelitian ini. Lima orang sebagai sampel kelompok sebelum radioterapi dan 5 orang sebagai sampel kelompok sesudah radioterapi. Dari masing-masing pasien diambil sel monosit dan serum darah tepi serta CSG. Kadar calprotectin diukur menggunakan metode ELISA. Hasil: kadar calprotectin pada kelompok sampel sebelum radioterapi lebih rendah dibandingkan kelompok sam pel sesudah radioterapi dilihat melalui sel monosit dan serum darah tepi. Sementara dari CSG, kadar calprotectin kelompok sampel sebelum radioterapi nampak lebih tinggi dibanding kelompok sesudah radioterapi. Hasil analisis statistik Anova menunjukkan perbedaan yang signifikan (p<0,05). Kesimpulan: terdapat perbedaan kadar calprotectin pada sel monosit, serum darah tepi dan CSG pasien KNF antara sebelum dan sesudah radioterapi. Pada sel monosit dan serum darah tepi, terjadi penurunan kadar calprotectin, sementara pada CSG terjadi peningkatan kadar calprotectin antara sebelum dan sesudah radioterapi.
THE INFLUENCE BETWEEN INJECTABLE PLATELET-RICH FIBRIN AND PLATELET-RICH PLASMA TOWARDS GINGIVAL FIBROBLAST CELL PROLIFERATION Arifia Anindita Danastri; Suryono Suryono; Kwartarini Murdiastuti
ODONTO : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.8.2.25-31

Abstract

ABSTRACTBackground: Gingiva is the outermost periodontal tissue that acts as a mechanical and biological barrier to the root of the teeth and alveolar bone. The main cellular elements in the gingiva are fibroblasts. Fibroblast cell proliferation is an important process in tissue regeneration. Growth factors that can stimulate fibroblast cell proliferation can be found in regenerative agents, such as injectable platelet-rich fibrin (i-PRF) and platelet-rich plasma (PRP). The aim of this study was to examine the influence between i-PRF and PRP on the gingival fibroblast cell proliferation in vitro study on primary cell culture.Method: Gingival fibroblast cell were obtained from primary cell culture derived from healthy gingiva. Ten mL of peripheral blood were centrifuged for i-PRF and PRP preparation. The samples were divided into three groups: i-PRF, PRP, and fibroblast cells without treatment. Cell proliferation were observed at day 1, day 3, day 5 using MTT assay at 550 nm. The data were analyzed by Two-Way ANOVA test, followed by Post Hoc test.Result: The results showed that the cell proliferation increased from day 1, 3, and 5 in all groups. The absorbance value of the cell proliferation in order from highest to lowest: i-PRF, PRP, and cell control.Conclusion: i-PRF and PRP increased the gingival fibroblast cell proliferation. i-PRF increased the cell proliferation higher than PRP.
EVALUATION OF FORMAT AND SECURITY OF DENTAL ELECTRONIC MEDICAL RECORD SYSTEMS IN GENERAL HOSPITAL BASED ON LEGISLATION Erdianto Setya Wardhana; Suryono Suryono; Ari Hernawan; Lukito Edi Nugroho
ODONTO : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.9.0.80-89

Abstract

Background: Electronic medical records in dentistry are the latest implementation in the health sector, especially dental health history to the services needed by patients by health care facilities and affect the format of dental, medical records and electronic medical record security systems. This study aimed to review the completeness of the medical record format and the electronic medical record security system of the dental polyclinic of the General Hospital in Batam City. Method: This study used a descriptive cross-sectional observational design with two samples. Processing data in the form of tables and texts and conclusions as a result of research. Result: The results showed incomplete dental electronic medical record formats at hospital A, such as patient identity, required medical data, odontogram, intraoral examination, and treatment chart, while Hospital B included patient identity, required medical data, intraoral analysis, treatment chart, and appendix. The two samples did not meet the integrity and non-repudiation aspects of the electronic medical record security system. Conclusion: According to the Indonesian Dental Medical Record Guidelines, both samples have an incomplete dental electronic medical record format and security system.
Evaluation of Antibacterial Potential of Carbonated Hydroxyapatite Combined with Propolis on Porphyromonas gingivalis Nungky Devitaningtyas; Ahmad Syaify; Dahlia Herawati; Suryono Suryono
Majalah Obat Tradisional Vol 25, No 1 (2020)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.55173

Abstract

Carbonated hydroxyapatite is ideal as a bone graft material because it has similar organic matters to the bone, excellent osteoconductive properties, and good biodegradation in the body. Hydroxyapatite contains the risk of being contaminated by bacteria called Porphyromonas gingivalis (P. gingivalis) in the oral cavity because it has no vascularization, therefore, facilitating adhesion of bacteria, and when applied in the oral cavity, it may cause an infection that then inhibits healing. Thus, it is necessary to use a material that has an antibacterial effect with low potential of causing resistance to treat the postsurgical infection properly. Propolis has antibacterial, antiviral, antifungal, antitumor, and immunomodulatory activities. Propolis contains a large number of flavonoids and phenols. The phenol compound in propolis is usually called caffeic acid phenethyl ester (CAPE), and it has a good antibacterial property. The study aims to evaluate the antibacterial effect of carbonated hydroxyapatite when immersed with different propolis concentrations of 2.5%, 5%, 7.5%, and 10% for 24 h and to measure the zone of inhibition against P. gingivalis. The Kruskal–Wallis test resulted in p = 0.00 (p < 0.05), indicating that there were significant differences among the test groups. The data processing was followed by Mann–Whitney U-test, and the results showed a significant difference in the group of carbonated hydroxyapatite-10 % propolis compared with the other groups. Inhibition zone of carbonated hydroxyapatite that immersed with propolis 10% showed the largest mean of diameters zone of inhibition.
Co-Authors Adiguna Putra Walianto Ahmad Syaify, Ahmad Al. Sri Koes Soesilowati Ari Hernawan Arifia Anindita Danastri Ayunda Nur Sukmawati Dahlia Herawati Desi Sandra Sari Erdianto Setya Wardhana Indi Kusumawati Islamy Rahma Hutami, Islamy Rahma Kwartarini Murdiastuti Leo Trido Saputro Lukito Edi Nugroho Marsetyawan HNES Marsetyawan HNES Noor Aziza Nungky Devitaningtyas Nur Rahman Ahmad Seno Aji Recita Indraswary Resi Manua Yassa Rurie Ratna Shantiningsih Sri Budi Barunawati Sri Kadarsih Sri Kadarsih Soejono Sri Pramestri Sudibyo Sudibyo Wayan T Artama Wayan T Artama