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All Journal The Indonesian Journal of Dental Research Majalah Kedokteran Gigi Indonesia ODONTO Dental Journal Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Majalah Obat Tradisional Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Odonto dental journal
Suryono Suryono
Department Of Periodontics, Faculty Of Dentistry, Universitas Gadjah Mada
Dentistry Health Professions Medicine & Pharmacology Public Health

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Carbonated Hydroxyapatite Containing Propolis as an Antibacterial Agent Candidate against Aggregatibacter actinomycetemcomitans Ayunda Nur Sukmawati; Sri Pramestri; Al. Sri Koes Soesilowati; Suryono Suryono
Majalah Obat Tradisional Vol 25, No 3 (2020)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.62712

Abstract

One of the periodontal pathogenic bacteria that can cause periodontitis and alveolar bone destruction is Aggregatibacter actinomycetemcomitans. An alveolar bone defect can be treated using a bone graft. Carbonated hydroxyapatite (CHA) is an alloplastic graft material. Alloplastic materials do not have vascularization, which will increase the risk of bacterial adhesion. Therefore, adding an antibacterial agent is needed to prevent bacterial adhesion, which will improve periodontal healing. Propolis is a natural ingredient that has broad-spectrum antibacterial activity and does not cause bacterial resistance. This study aimed to assess the antibacterial activity of carbonated hydroxyapatite after being incorporated with propolis against Aggregatibacter actinomycetemcomitans. Carbonated hydroxyapatite was embedded into four different concentrations of propolis solution (2.5%, 5%, 7.5%, and 10%). An antimicrobial assay against Aggregatibacter actinomycetemcomitans was done using the disc diffusion test method. The inhibition zone was measured to determine the antibacterial ability of the specimens. The inhibition zone was found on the carbonated hydroxyapatite incorporated with propolis at all concentrations. Carbonated hydroxyapatite incorporated with 10% propolis showed the largest inhibition zone. Data analysis using the Kruskal–Wallis test showed a significant difference between the groups tested (p <.05). In conclusion, carbonated hydroxyapatite incorporated with propolis has antibacterial activity against Aggregatibacter actinomycetemcomitans
Detection of aggressive periodontitis by calprotectin expression Desi Sandra Sari; Suryono Suryono
Dental Journal (Majalah Kedokteran Gigi) Vol. 42 No. 4 (2009): December 2009
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.275 KB) | DOI: 10.20473/j.djmkg.v42.i4.p185-188

Abstract

Background: Calprotectin is a calcium-binding protein expressed by neutrophil, monocytes, gingival keratinocytes, and oral epithelial cells. The concentrations of calprotectin increase in plasma, urine and synovial fluid of patients with inflammatory diseases. This protein is known as a marker for periodontal diseases and is detected in gingival crevicular fluids. Purpose: This study was aimed to investigate the detection of inflammation on the aggressive periodontitis by calprotectin expression. Method: The gingival crevicular fluids were taken from five aggressive periodontitis patients and five healthy subjects by using sterile paper points. Calprotectin expression was analyzed by ELISA technique. Result: The results showed the significant difference in calprotectin expression between subject with aggressive periodontitis and healthy subjects p = 0.002 (p < 0.05). Conclusion: It was concluded that the calprotectin expression on the aggressive periodontitis patients may be useful for evaluation the progression of inflammation in periodontitis.
Calprotectin mRNA (MRP8/MRP14) expression in neutrophils of periodontitis patients with type 2 diabetes mellitus Ahmad Syaify; Marsetyawan HNES; Sudibyo Sudibyo; Suryono Suryono
Dental Journal (Majalah Kedokteran Gigi) Vol. 42 No. 3 (2009): September 2009
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (648.763 KB) | DOI: 10.20473/j.djmkg.v42.i3.p130-133

Abstract

Background: Calprotectin, a major cytosolic protein of leukocytes, is detected in neutrophils and monocytes/machrophages. This protein is known to be a marker for several inflammatory diseases including periodontitis. In type 2 diabetes mellitus patients, the severity of periodontitis was strongly thought to be caused by decreasing of leukocytes function such as neutrophils. Previous research found that the calprotectin level in serum of periodontitis patients with type 2 DM is higher than periodontits patients non DM. Purpose: The aim of this study was to determine calprotectin mRNA (MRP8/MRP14) expression in human neutrophils of periodontitis patients with type 2 diabetes mellitus. Methods: Neutrophils were isolated from the peripheral blood of periodontitis patients with uncontrolled type 2 DM, controlled type 2 DM, and non DM. The expression of calprotectin mRNA (MRP8 and MRP14) were detected by RTPCR. Result: The result showed that the value of mRNA calprotectin expression in DM patients were higher than non DM, and the highest expression was on the uncontrolled type 2 DM. Conclusion: The basal level of calprotectin mRNA MRP8/MRP14 expression increased in neutrophil of periodontitis patient with type 2 DM compared non diabetic subjects. It was suggested that high basal level of calprotectin mRNA has a role in the regulation of periodontitis severity with diabetes mellitus patients.
Antibacterial Effects of Ethanolic Extract of Bidara (Ziziphus mauritiana Lam) Leaf Against Porphyromonas gingivalis Noor Aziza; Islamy Rahma Hutami; Recita Indraswary; Suryono Suryono
Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva Vol 11, No 2 (2022): November
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/di.v11i2.15222

Abstract

The bidara plant (Ziziphus mauritiana Lam) is widely distributed in various Asian countries. Bidara leaves contain secondary metabolites, the main content of which is flavonoids. As a gram-negative anaerobic bacteria, Porphyromonas gingivalis is one of the normal flora of the oral cavity. However, over quantities of this bacteria can promote chronic periodontitis. This research aims to analyze the bidara leaf ethanolic extract as an inhibitory agent of Porphyromonas gingivalis. This research design is experimental laboratory research with a post-test controlled group of Porphyromonas gingivalis inhibition. A total of 25 samples consisted of 5 groups of ethanol extract of bidara leaves at concentrations of 1%, 3%, 9%, positive control betel leaves, and negative control aquadest. Bacteria incubation was held for 48 hours, and the free bacterial zone was analyzed by the One Way ANOVA test. The results of the analysis showed that there was a significant difference between the control group and the treatment group. This study concludes that the ethanol extract of bidara leaves had a strong inhibitory effect on Porphyromonas gingivalis.
Antibacterial potential from periodontal dressing raw propolis Trigona itama bee based against Porphyromonas gingivalis: experimental research Suryono Suryono; Resi Manua Yassa; Leo Trido Saputro; Adiguna Putra Walianto; Nur Rahman Ahmad Seno Aji
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 35, No 1 (2023): April 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v35i1.46229

Abstract

ABSTRACTIntroduction: One of the procedures used in dentistry to address periodontal disease is periodontal surgery, which can lead to open wounds. Periodontal dressing is a dental material that can help with this issue by accelerate the healing of wounds. The aim of the in vitro study was to investigate the potential inhibition of periodontal dressing with the basic formulated from raw propolis Trigona itama bees against Porphyromonas gingivalis bacteria at various rates. Methods: This in vitro study was true experimental study with Porphyromonas gingivalis cultured in agar plate as sample which sample size was calculated using Federer formula. Periodontal dressing with raw propolis formulations were divided into eight groups of 85, 80, 75, 70, 65, 62.5, 60 and 57%, which were then compared with the 100% raw propolis, RESO-PAC™ group, COE-PAK™, and Baer formulations. Antibacterial testing was tested using zone of inhibition test and was calculated by adding up the zone of inhibition of Porphyromonas gingivalis. The data results were then analyzed using one-way ANOVA (p<0.05). Results: The Shapiro-Wilk test results for the normality test show a significance level of 0.05. It means that the data is commonly distributed. the homogeneity test using Levene's statistics has a significance level of p>0.05. This means the data homogenous distributed. The results of statistical analysis using one-way ANOVA showed a p-value=0.001 (p<0.05), which means that there was a significant difference between the treatment groups. The results showed that pure raw propolis had the highest antibacterial with an inhibition zone diameter of 3.4667 mm after RESO-PAC™, followed by periodontal dressing with raw propolis 85% with an inhibition zone of 2.9167 mm, and periodontal dressing with raw propolis 80% with an inhibition zone of 2.5167 mm. However, at concentrations of 75 to 57%, no bacterial inhibition zone was found. Conclusion: Periodontal dressing with raw propolis formulation 85% had the highest antibacterial activity of Porphyromonas gingivalis after 100% raw propolis and RESO-PAC™.Keywords: Trigona itama, antibacterial assay, periodontal dressing, raw propolisPotensi antibakteri periodontal dressing propolis mentah lebah Trigona itama terhadap Porphyromonas gingivalis: Penelitian eksperimental ABSTRAKPendahuluan: Salah satu prosedur yang dapat digunakan untuk merawat penyakit periodontal adalah bedah periodontal yang akan mengakibatkan luka terbuka pada daerah operasi. Periodontal dressing adalah suatu material kedokteran gigi yang dapat membantu dalam proses penyembuhan luka pasca bedah periodontal. Tujuan dari penelitian in vitro ini adalah mengetahui potensi inhibisi periodontal dressing dari formulasi propolis mental lebah Trigona Itama pada Porphyromonas gingivalis dalam beberapa konsentrasi. Metode: Studi ini merupakan penelitian eksperimental murni dengan sampel penelitian bakteri Porpohyromonas gingivalis yang dikultur pada plat agar dengan perhitungan sampel dilakukan menggunakan rumus Federer. Studi ini membagi periodontal dressing propolis mentah menjadi 8 kelompok: 85, 80, 75, 70, 65, 62,5, 60, dan 57% yang dibandingkan dengan propolis mentah 100%, RESO-PAC™, COE-PAK™, dan Baer Formulation. Pengukuran antibakteri dilakukan menggunakan uji zona inhibisi Porphyromonas gingivalis yang dianalisa menggunakan one-way ANOVA (p<0.05). Hasil: Hasil uji normalitas dengan menggunakan uji Shapiro-Wilk menunjukkan nilai signifikansi p>0,05. Hal ini berarti data berdistribusi normal, Hasil uji homogenitas dengan levene’s statistic menunjukkan nilai signifikansi p>0,05, hal ini menunjukan bahwa data homogen. Hasil analisis statistik dengan menggunakan one way ANOVA menunjukkan p-value=0,001 (p<0,05) yang berarti terdapat perbedaan yang bermakna antar kelompok perlakuan. Hasil penelitian menunjukkan propolis mentah memiliki potensi antibakteri paling tinggi dengan diameter zona inhibisi 3.4667 mm setelah RESO-PAC™k, diikuti propolis mentah 85% dengan diameter 2.9167 mm dan propolis mentah 80% dengan diameter 2.5167 mm. Tidak ditemukan zona inhibisi bakteri pada konsentrasi 57% hingga 75%. Simpulan: Periodontal dressing menggunakan konsentrasi 85% memiliki aktivitas antibakteri tertinggi pada Porphyromonas gingivalis setelah propolis mentah 100% dan RESO-PAC™.Kata Kunci: Trigona itama, pengukuran antibakteri, periodontal dressing, propolis mentah
EVALUATION OF FORMAT AND SECURITY OF DENTAL ELECTRONIC MEDICAL RECORD SYSTEMS IN GENERAL HOSPITAL BASED ON LEGISLATION Erdianto Setya Wardhana; Suryono Suryono; Ari Hernawan; Lukito Edi Nugroho
Odonto : Dental Journal Vol 9: Special Issue 1. April 2022
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.606 KB) | DOI: 10.30659/odj.9.0.80-89

Abstract

Background: Electronic medical records in dentistry are the latest implementation in the health sector, especially dental health history to the services needed by patients by health care facilities and affect the format of dental, medical records and electronic medical record security systems. This study aimed to review the completeness of the medical record format and the electronic medical record security system of the dental polyclinic of the General Hospital in Batam City. Method: This study used a descriptive cross-sectional observational design with two samples. Processing data in the form of tables and texts and conclusions as a result of research. Result: The results showed incomplete dental electronic medical record formats at hospital A, such as patient identity, required medical data, odontogram, intraoral examination, and treatment chart, while Hospital B included patient identity, required medical data, intraoral analysis, treatment chart, and appendix. The two samples did not meet the integrity and non-repudiation aspects of the electronic medical record security system. Conclusion: According to the Indonesian Dental Medical Record Guidelines, both samples have an incomplete dental electronic medical record format and security system.
Co-Authors Adiguna Putra Walianto Ahmad Syaify, Ahmad Al. Sri Koes Soesilowati Ari Hernawan Ayunda Nur Sukmawati Dahlia Herawati Desi Sandra Sari Erdianto Setya Wardhana Indi Kusumawati Islamy Rahma Hutami, Islamy Rahma Leo Trido Saputro Lukito Edi Nugroho Marsetyawan HNES Marsetyawan HNES Noor Aziza Nungky Devitaningtyas Nur Rahman Ahmad Seno Aji Recita Indraswary Resi Manua Yassa Rurie Ratna Shantiningsih Sri Budi Barunawati Sri Kadarsih Sri Kadarsih Soejono Sri Pramestri Sudibyo Sudibyo Wayan T Artama Wayan T Artama