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Journal : JURNAL BIOLOGI INDONESIA

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Fenotipe Virus Avian Influenza (AI) Subtipe H5N1 Berbeda Karakter Genetik di Indonesia Dharmayanti, NLP Indi; Indriani, Risa; Hartawan, Risza; Ratnawati, Atik
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.3031

Abstract

In Indonesia, data on the phenotype of AI virus subtype H5N1 is very limited, due to the facilities for such testingshould be performed in a laboratory Biosafety level III. Additionally influenza virus has a high error rate duringtranscription of their genome has low RNA polymerase. High error rates generate quasispecies. In this study, wedid some H5N1 viruses infection with different genetic variations and conducted DNA sequencing in severalorgans. To determine the possible emergence detection of quasispecies in different organs and until the virus wasexcreted. We tested the hypothesis that the quasispecies might arise from virus that was infected to theexperimental animals. The results of this study showed that the AI virus subtype H5N1 had different phenotypes inanimal depending on their genetic character. AI virus subtype H5N1 killed chickens within 48-72 hoursdepending on the genetic character of the virus. New AI viruses of subtype H5N1 caused more severe organdamage severe than the character of the old AI viruses. Adaptation of virus in each organ also proved that the viruslikely create variations/quasispecies, which was different from the viral origin.Keywords: Phenotypes, avian influenza, H5N1, genetic character, quasispecies
Efikasi Vaksin Inaktif Bivalen Avian Influenza Virus Subtipe H5N1 (Clade 2.1.3. dan Clade 2.3.2) di Indonesia Dharmayanti, NLP Indi; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1108.821 KB) | DOI: 10.14203/jbi.v11i2.2190

Abstract

ABSTRACTStatus of avian influenza virus subtype H5N1 in Indonesia until 2014 is still endemic in poultry and recorded, there were two types clade of circulating H5N1 namely clade 2.1.3 and the new introduction of lade 2.3.2 since the end of 2012. Both of the clade of avian influenza viruses subtype H5N1 (clade 2.1.3 and 2.3.2) caused the the  AI vaccination program to control of AI in poultry needs to be evaluated.  In this study, we developed a bivalent AI vaccine (which contains clade 2.1.3 and 2.3.2 viruses as a seed vaccine) that   adapted with  the circulation of AI viruses in the field. Result of the study showed that the bivalent vaccine which developed in this study has good efficacy that was challanged with both of AI clade AI and proven to reduce shedding / viral contamination to the environment. It is expected that  the development of bivalent H5N1 vaccine will increase the effectiveness and efficacy of vaccination programs to control highly pathogenic avian influenza disease in Indonesia. Keywords : avian influenza virus, clade, vaccine, bivalent 
Studi Efikasi Vaksin Bivalen AI Isolat Lokal terhadap Beberapa Karakter Genetik Virus AI subtipe H5N1 Indriani, Risa; Dharmayanti, NLP Indi
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.942 KB) | DOI: 10.14203/jbi.v9i1.143

Abstract

Studi vaksin inaktif bivalen AI isolat lokal subtipe H5N1 terhadap beberapa karakter genetik virus AI H5N1 padaayam layer dan broiler. Vaksin inaktif bivalen dari isolat lokal AI A/Ck/west java/Smi-M6/2008 and A/Ck/westjava/Pwt-D10-39/2010. Ayam layer dan broiler komersial divaksinasi dengan vaksin inaktif bivalen AI isolat lokal,setelah 3 minggu vaksinasi ditantang dengan virus AI A/Ck/west java/Smi-Part/2006, A/Ck/west java/Subang-JAPFA-29/2007 and A /Ck/west java/Smi-Rahm2/2011. Ayam layer vaksinasi mendapat perlindungan dari morbiditas,mortalitas dan penurunan ekskresi virus tantang dengan tingkat proteksi 90-100% sedangkan ayam layerkontrol mati dalam waktu 2-3 hari, sementara broiler yang divaksinasi tidak mendapatkan perlindungan dari morbiditasdan mortalitas setelah terinfeksi virus AI tantang. Hasil studi memperlihatkan vaksin inaktif bivalen AI isolatlokal subtipe H5N1 mampu memberikan perlindungan pada ayam layer dari infeksi beberapa karakter genetikvirus AI subtipe H5N1 .Kata kunci: Vaksin bivalen, subtipe H5N1, tantang dan proteksi
Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Antibody in Chickens Using Local Isolate of PTS III Indriani, Risa; Indi Dharmayanti, NLP.
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.167 KB) | DOI: 10.14203/jbi.v9i2.162

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectiousbronchitis (IBV). Antigen was prepared from whole virus of infectious bronchitis local isolate PTS-III serotype.Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera indeveloped ELISA were determined 0.4μg/well, 1:2000 and 1:100, respectively. Correlation optical densities (OD)were compared with a standard commercial ELISA (R2=0.933). The developed ELISA has a better sensitivity to hemagglutinationinhibition (HI) test. The developed local isolate ELISA can be used to detect antibody against infectiousbronchitis virus and it is suitable for sample screening at the diagnostic laboratories.Keywords: ELISA, antibody, chicken, IB PTS-III local isolate
Respon Titer Antibodi dan Proteksi Virus Newcastle Disease Genotype I, II, VI dan VII Sebagai Vaksin Terhadap Infeksi Isolat Virus Newcastle Disease Chicken/Indonesia/GTT/11. Indriani, Risa; Dharmayanti, NLP Indi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1354.779 KB) | DOI: 10.14203/jbi.v12i2.2887

Abstract

ABSTRACTThe Newcastle Disease (ND) virus causes the most important disease in chicken, and very contagiuos in poultry in many countries. In Indonesia the Newcastle Disease or is called tetelo became endemic in chicken currently, the ND virus of genotype VII caused morbidity 100% and mortality 80% in chicken. The fusion and haemaglutinin-neuraminidase protein are involved in adhesion to the surface of hert cells to antibodies. HN and F protein induced by the ND vaccines have neutralizing effects. The aim of this study was to compare the antibody level and protection of several ND vaccine genotypes (genotype I, II, VI and VII) in chicken against ND virus chicken / Indonesia/GTT/11 genotype VII. Our result showed that all genotype ND vaccines (I, II, VI dan VII) produce 100% protection in chicken from clinical and mortality, but the challenge virus excretion in chickens vaccinated with ND vaccine VII genotypes were significantly different (p <0.05) to other groups. Response of antibody in chicken vaccinated ND genotype VII show highest mean titer (GMT 122) compered other ND vaccine. In onclusion, the ND virus chicken/Indonesia/ GTT/11 genotype VII is good to be used as inactive vaccine seed, which appropriate to the field virus circulating at this time.Keywords: virus, Newcastle Disease, genotype, vaccine
Sirkulasi virus Avian influenza H5N1 Tahun 2010 : Virus genetic drift mirip A/Ck/West Java/Pwt-Wij/2006 ditemukan di beberapa kabupaten di Sumatra dan Jawa Dharmayanti, NLP Indi; Ratnawati, Atik; Hewajuli, Dyah Ayu; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2654.808 KB) | DOI: 10.14203/jbi.v8i1.3069

Abstract

The avian influenza H5N1 virus circulation in 2010 : Genetic Drift Like Virus A/Chicken/West Java/Pwt-Wij/2006 was found in several districts of Sumatra and Java. Until 2011, the H5N1 subtype of AI virus is still circulating in many parts of Indonesia. The discovery of the AI viruses which have undergone genetic drift since 2006 until now requires serious attention from the government in terms of AI disease control, the surveillance and monitoring of virus circulation and execution of genetic mapping to determine the genetic character of the AI virus at the molecular level, especially on the surface of glycoproteins (HA and NA protein).This information is needed to determine the diversity and character of the AI virus in Indonesia. Genetic data are used to evaluate the strategy to control AI in Indonesia, such as vaccination and the vaccine seed used and determine the extent of AI virus mutation in Indonesia has beenmutated. This study conducted by monitoring of the AI virus circulation throughout 2010. The methods used were AI virus isolation, RT-PCR, sequencing of genes coding for viral surface and the prediction of three-dimensional analysis to determine the location of virusmutation. The results of this study showed that most of the AI virus subtype H5N1, which was isolated during the year 2010, showed similar mutations to the genetic drift virus in 2006, A /Ck/West Java/Pwt-Wij/2006. The viruses were characterized by the presence of 18-19 amino acid substitutions at the level of the HA protein. On the NA protein level, there is a single mutation which was buried in the NA molecule. This mutation probably did not influence for NA activity. Genetic mapping of AI virus subtype H5N1 in 2010 showed that the viral genetic driftas the mutan virus A/Ck/West Java/Pwt-Wij/2006 have circulated not only in West Jawa alone but has been found on the island of Sumatra, Banten, West Jawa and East Jawa.Keywords: Circulation, avian influenza H5N1 virus, genetic drift
Ekspresi Sitokin Tumor Necrosis Factor (TNF-α) dan Interferon (IFN-γ) pada sel MDCK yang diinfeksi virus avian influenza subtipe H5N1 asal Indonesia Dharmayanti, NLP. Indi; Rillah, Ukhti Dwi; Syamsiah, Farida; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (896.502 KB) | DOI: 10.14203/jbi.v10i1.328

Abstract

Patients with H5N1 disease have a characteristic complications of pneumonia with acute respiratory syndromedisterss and multi-organ failure associated with cytokine dysregulation, so it is suspected that the clinical forms ofsevere H5N1 disease in humans is caused by dysregulation of cytokine induction of H5N1 virus. Study oncytokines associated with H5N1 influenza virus infection is still limited, while animal studies using virus H5N1/97give conflicting results. Recent studies, showing that in fact the high cytokine is not a common form of the H5N1viruses, so Pathogenecity of H5N1 viruses were not only determined by whether or not the virus can inducehypercytokinemia. In this study we wanted to determine the phenotype of the Indonesia H5N1 virus in inducingcytokine expression. This study compared three Indonesian H5N1 viruses that have different genetic variations ofthe virus including the clade 2.1.1 and two other viruses included in clade 2.1.3 that were infected in Madin DarbyCanine Kidney (MDCK) cell. The MDCK cell that infected virus were observed for 72 hours. We observed theinfection during 72 hours and the cytokines expression were examined using RT-PCR method. The results showedthat the H5N1 influenza viruses used in this study showed the low expression of cytokines IFN-γ, and did notshow any expression of the TNF-α cytokines in MDCK cells infected with the H5N1 virus within 72 hours afterinfection.Keywords : Expression, H5N1 virus, Cytokines, sel Madin Darby Canine Kidney (MDCK)
Cross-Reaction of Duck and Chicken Sera against Avian Influenza H5N1 Virus Clades 2.1.3 and 2.3.2 Antigens by Hemagglutination Inhibition Test Indriani, Risa; Dharmayanti, NLP I; Martindah, E.
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (607.375 KB) | DOI: 10.14203/jbi.v13i2.3407

Abstract

ABSTRACTThis study aims to determine the cross-reaction between the antigen of avian influenza (AI) H5N1 virus clades 2.1.3 and 2.3.2 in duck and chicken sera, which were vaccinated with inactivated AI H5N1 clade 2.1.3 vaccine against AI H5N1 clade 2.3.2 antigen and those vaccinated with inactivated AI H5N1 clade 2.3.2 vaccine against H5N1 clade 2.1.3 antigen. The sera tested were obtained from postvaccination and control (unvaccinated) chickens and ducks in the laboratory condition, and from AI H5N1 postvaccination ducks in the field condition. HI test was conducted by using AI H5N1 clades 2.1.3 and 2.3.2 antigens. The results of HI titer were analyzed by the geometric means and by ANOVA. The results show that cross-reactions in both chicken and duck sera after AI H5N1 clade 2.3.2 vaccination tested with AI H5N1 clade 2.1.3 antigen occurred with low antibody titers, whereas in chicken and duck sera postvaccination with avian influenza H5N1 virus clade 2.1.3 showed cross-reaction with high antibody titer against clade 2.3.2 antigen. The conclusion of this study, postvaccination sera of AI H5N1 clade 2.1.3 provide better cross-reaction compared to the postvaccination sera of AI H5N1 clade 2.3.2.Keywords: sera, chicken, duck, AI H5N1, clade 2.1.3 and clade 2.3.2.
Identification and Characterization of Infectious Bronchitis Virus (IBV) in Indonesia Dharmayanti, Ni Luh Putu Indi; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3095

Abstract

ABSTRACTInfectious Bronchitis Virus (IBV) is an acute viral and highly infectious disease which attacks the respiratory system of chicken. The impact of economic losses is very large since it decreases the egg production and itcauses the abnormal of egg shapes, decreased of body weight and death. In Indonesia, the IBV disease is still a serious problem in poultry regarding to many variants which arise from mutations of the IB virus. The decreasing possibility of the vaccination effectiveness due to incompatibility of strains used towards the virus circulated in the field. The variants of IB virus have not been well-documented in Indonesia since the lack of characterization of this virus. In this study, we characterize the IB virus isolates obtained from our local outbreaks in the field in order to determine the variant of IB virus in Indonesia and provide recommendations for the improvements of IB vaccine seed viruses which is adapted to IB virus circulating in the field. The IB virus characterized in this study shows close genetic relationship with the IB virus from Taiwan and China. However, it did not correlate with the local IB virus that has been found previously.Keywords: Identification, Character, IBV, Indonesia
FENOTIPE VIRUS AVIAN INFLUENZA (AI) SUBTIPE H5N1 BERBEDA KARAKTER GENETIK DI INDONESIA Dharmayanti, NLP Indi; Indriani, Risa; Hartawan, Risza; Ratnawati, Atik
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.3031

Abstract

In Indonesia, data on the phenotype of AI virus subtype H5N1 is very limited, due to the facilities for such testingshould be performed in a laboratory Biosafety level III. Additionally influenza virus has a high error rate duringtranscription of their genome has low RNA polymerase. High error rates generate quasispecies. In this study, wedid some H5N1 viruses infection with different genetic variations and conducted DNA sequencing in severalorgans. To determine the possible emergence detection of quasispecies in different organs and until the virus wasexcreted. We tested the hypothesis that the quasispecies might arise from virus that was infected to theexperimental animals. The results of this study showed that the AI virus subtype H5N1 had different phenotypes inanimal depending on their genetic character. AI virus subtype H5N1 killed chickens within 48-72 hoursdepending on the genetic character of the virus. New AI viruses of subtype H5N1 caused more severe organdamage severe than the character of the old AI viruses. Adaptation of virus in each organ also proved that the viruslikely create variations/quasispecies, which was different from the viral origin.Keywords: Phenotypes, avian influenza, H5N1, genetic character, quasispecies
Co-Authors A Wiyono Ahpas, Ahpas Atik Ratnawati Bambang Ngaji Utomo Darminto . Dewiyanti, Rina Dyah Ayu Hewajuli E. Martindah Eny Martindah Fairusya, Nuha Farida Syamsiah, Farida Fitrine Ekawasti, Fitrine Helmy Hamid Hoerudin, Heri Indi Dharmayanti Indi Dharmayanti, NLP. Indi Dharmayanti, NLP. Izzah, Ukhtul J. Ignjatovic, J. Jejen Jaelani L Parede Lies Parede Muharam Saepulloh N.L.P Indi Dharmayanti N.L.P.I Dharmayanti N.L.P.I. Dharmayanti NI LUH PUTU INDI DHARMAYANTI NLP I Dharmayanti NLP Indi Dharmayanti NLP. Indi Dharmayanti, NLP. Indi NLPI Dharmayanti Nuradji, Harimurti Putri, Ajeng Fabeana R.M Abdul Adjid R.M.A. Adjid Rahmat Setya Adji RISZA HARTAWAN S Wahyuwardani sekarmila, Gita Simson Tarigan Sukarsih . Supriyanto Supriyanto Suryatmiati, Sri Trianita, Desi Ukhti Dwi Rillah, Ukhti Dwi Wawan Gunawan Winarsongko, Agus yuda pratama