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All Journal Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences JURNAL BIOLOGI INDONESIA Jurnal Kedokteran Hewan Jurnal Medika Veterinaria
RISA INDRIANI
Balai Besar Penelitian Veteriner
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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SIRKULASI VIRUS AVIAN INFLUENZA H5N1 TAHUN 2010 : VIRUS GENETIC DRIFT MIRIP A/CK/WEST JAVA/PWT-WIJ/2006 DITEMUKAN DI BEBERAPA KABUPATEN DI SUMATRA DAN JAWA Dharmayanti, NLP Indi; Ratnawati, Atik; Hewajuli, Dyah Ayu; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3069

Abstract

The avian influenza H5N1 virus circulation in 2010 : Genetic Drift Like Virus A/Chicken/West Java/Pwt-Wij/2006 was found in several districts of Sumatra and Java. Until 2011, the H5N1 subtype of AI virus is still circulating in many parts of Indonesia. The discovery of the AI viruses which have undergone genetic drift since 2006 until now requires serious attention from the government in terms of AI disease control, the surveillance and monitoring of virus circulation and execution of genetic mapping to determine the genetic character of the AI virus at the molecular level, especially on the surface of glycoproteins (HA and NA protein).This information is needed to determine the diversity and character of the AI virus in Indonesia. Genetic data are used to evaluate the strategy to control AI in Indonesia, such as vaccination and the vaccine seed used and determine the extent of AI virus mutation in Indonesia has beenmutated. This study conducted by monitoring of the AI virus circulation throughout 2010. The methods used were AI virus isolation, RT-PCR, sequencing of genes coding for viral surface and the prediction of three-dimensional analysis to determine the location of virusmutation. The results of this study showed that most of the AI virus subtype H5N1, which was isolated during the year 2010, showed similar mutations to the genetic drift virus in 2006, A /Ck/West Java/Pwt-Wij/2006. The viruses were characterized by the presence of 18-19 amino acid substitutions at the level of the HA protein. On the NA protein level, there is a single mutation which was buried in the NA molecule. This mutation probably did not influence for NA activity. Genetic mapping of AI virus subtype H5N1 in 2010 showed that the viral genetic driftas the mutan virus A/Ck/West Java/Pwt-Wij/2006 have circulated not only in West Jawa alone but has been found on the island of Sumatra, Banten, West Jawa and East Jawa.Keywords: Circulation, avian influenza H5N1 virus, genetic drift
EKSPRESI SITOKIN TUMOR NECROSIS FACTOR (TNF-α) DAN INTERFERON (IFN-γ) PADA SEL MDCK YANG DIINFEKSI VIRUS AVIAN INFLUENZA SUBTIPE H5N1 ASAL INDONESIA Dharmayanti, NLP. Indi; Rillah, Ukhti Dwi; Syamsiah, Farida; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i1.328

Abstract

Patients with H5N1 disease have a characteristic complications of pneumonia with acute respiratory syndromedisterss and multi-organ failure associated with cytokine dysregulation, so it is suspected that the clinical forms ofsevere H5N1 disease in humans is caused by dysregulation of cytokine induction of H5N1 virus. Study oncytokines associated with H5N1 influenza virus infection is still limited, while animal studies using virus H5N1/97give conflicting results. Recent studies, showing that in fact the high cytokine is not a common form of the H5N1viruses, so Pathogenecity of H5N1 viruses were not only determined by whether or not the virus can inducehypercytokinemia. In this study we wanted to determine the phenotype of the Indonesia H5N1 virus in inducingcytokine expression. This study compared three Indonesian H5N1 viruses that have different genetic variations ofthe virus including the clade 2.1.1 and two other viruses included in clade 2.1.3 that were infected in Madin DarbyCanine Kidney (MDCK) cell. The MDCK cell that infected virus were observed for 72 hours. We observed theinfection during 72 hours and the cytokines expression were examined using RT-PCR method. The results showedthat the H5N1 influenza viruses used in this study showed the low expression of cytokines IFN-γ, and did notshow any expression of the TNF-α cytokines in MDCK cells infected with the H5N1 virus within 72 hours afterinfection.Keywords : Expression, H5N1 virus, Cytokines, sel Madin Darby Canine Kidney (MDCK)
Detection of Very Virulent Infectious Bursal Disease (IBD) in Chicken in West Java Putri, Ajeng Fabeana; Winarsongko, Agus; Hoerudin, Heri; sekarmila, Gita; Ahpas, Ahpas; Jaelani, Jejen; Gunawan, Wawan; Dewiyanti, Rina; Pratama, Yuda; Nuradji, Harimurti; Fairusya, Nuha; Ekawasti, Fitrine; Adji, Rahmat Setya; Dharmayanti, NLP Indi; Indriani, Risa; Utomo, Bambang Ngaji; Suryatmiati, Sri
Jurnal Medika Veterinaria Vol 18, No 1 (2024): J.Med.Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v18i1.35819

Abstract

Infectious Bursal Disease (IBD), also known as Gumboro disease, is an acute, highly contagious disease that infects chickens and causes a high mortality rate of up to 100% in young animals. The disease is caused by Infectious Bursal Disease Virus (IBDV) of the genus Avibirnavirus, family Birnaviridae. The disease has been reported in Indonesia since 1976, and management strategies for the disease, such as vaccination, have been applied to prevent and control outbreaks in poultry farms. In this study, we conducted the detection of the disease in chickens from a farm in West Java with a mortality rate of 80%. Chickens showing clinical signs, such as sudden death, anorexia, watery diarrhea, and ruffled feathers, were necropsied, and organ samples, including the bursa Fabricius, brain, and spleen, were collected. The samples were then tested using Reverse-Transcriptase Polymerase Chain Reaction (RT-PCR) to confirm the diagnosis of IBD. Positive results were obtained in this study, highlighting the need for improved biosecurity in poultry farms in Indonesia. These results also provided a basis for further research on viral characterization to develop detection kits or vaccines for IBD using local isolates from the field in Indonesia.
IDENTIFIKASI MOLEKULER VIRUS SUBTIPE H3 DAN H10 PADA UNGGAS Dharmayanti, Indi; Indriani, Risa
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1249

Abstract

Penelitian ini bertujuan mengidentifikasi molekuler virus-virus influenza yang tidak dapat diidentifikasi dengan primer H5N1 sehingga perlu dilakukan identifikasi lanjutan dengan primer virus influenza lainnya untuk mengetahui jenis subtipe virus influenza yang bersirkulasi di lapang. Sampel yang digunakan adalah lima sampel cairan alantois (Kode B1-B5) yang diduga mengandung virus influenza selain virus H5N1. Metode yang digunakan pada penelitian ini adalah dengan reverse transcription polymerase chain reaction (RT-PCR) menggunakan beberapa set primer influenza dan konfirmasi dilakukan dengan pengurutan deoxyribonucleic acid (DNA). Dari hasil analisis RT-PCR dan pengurutan DNA menunjukkan bahwa sampel dengan kode B1 dan B2 menunjukkan homologi tertinggi dengan data virus yang terdaftar di GenBank (NCBI) yaitu dengan virus influenza subtipe H3 sedangkan sampel dengan kode B4 mempunyai homologi tertinggi dengan virus influenza subtipe H10. Dari identifikasi dan karakterisasi tersebut kemungkinan bahwa virus sampel kode B1 (A/Chicken/Buleleng/BBVD488-9/2009) dan (B2) A/Duck/Tabanan/BBVD573-10/2009 adalah virus (AI) subtipe H3 sedangkan sampel kode B4 (A/Chicken/Klungkung/ BBVD006-1/2010) adalah virus AI subtipe H10.
Co-Authors A Wiyono Ahpas, Ahpas Atik Ratnawati Bambang Ngaji Utomo Darminto . Dewiyanti, Rina Dyah Ayu Hewajuli E. Martindah Eny Martindah Fairusya, Nuha Farida Syamsiah, Farida Fitrine Ekawasti, Fitrine Helmy Hamid Hoerudin, Heri Indi Dharmayanti Indi Dharmayanti, NLP. Indi Dharmayanti, NLP. J. Ignjatovic, J. Jejen Jaelani L Parede Lies Parede Muharam Saepulloh N.L.P Indi Dharmayanti N.L.P.I Dharmayanti N.L.P.I. Dharmayanti NI LUH PUTU INDI DHARMAYANTI NLP I Dharmayanti NLP Indi Dharmayanti NLP. Indi Dharmayanti, NLP. Indi NLPI Dharmayanti Nuradji, Harimurti Putri, Ajeng Fabeana R.M Abdul Adjid R.M.A. Adjid Rahmat Setya Adji RISZA HARTAWAN S Wahyuwardani sekarmila, Gita Simson Tarigan Sukarsih . Suryatmiati, Sri Ukhti Dwi Rillah, Ukhti Dwi Wawan Gunawan Winarsongko, Agus yuda pratama