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Seroprevalence of highly pathogenic avian influenza H5 subtype clade 2.3.2 on ducks and muscouvy ducks in small holders farm Martindah, Eny; Indriani, Risa; Wahyuwardani, S
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 4 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.717 KB) | DOI: 10.14334/jitv.v19i4.1097

Abstract

Seroprevalence studies of HPAI H5 subtype in ducks and muscouvy duck in smallholders farm was carried out in Serang and Tangerang District, Banten Province. The study comprised a serological survey to define the distribution and prevalence of HPAI H5 subtype infection on ducks and muscouvy ducks as well as attempted isolation of the virus from these species. Unit of sample in each stage was randomly choosen by multy stage random sample. Blood samples were taken from ducks and muscouvy ducks that had never been vaccinated, purposively. Sera were tested using Haemaglutination Inhibition Test, antigen H5N1 (clade 2.3.2), while the cloaca and trachea swab samples was injected into specific pathogens free (SPF) embryonated 9-11 days old, to isolate the virus. Results showed that H5 subtype virus could be isolated from tracheal swabs of ducks in the various age groups. The seroprevalence of H5 subtype virus in Banten Province was 25.5%, in which, 24.3% occured in ducks and in muscouvy duck in the rate of 1.2%, with titer HI positive was > 3log2. Based on species, seroprevalence level HPAI H5 subtype in ducks was 3-4 times higher than the level of seroprevalence of HPAI H5 subtype in muscouvy duck, which indicated that the H5 subtype virus more likely to circulate in the ducks flock than in muscouvy duck. This study noted that both muscouvy duck and ducks appeared to play a significant role in the epidemiology of the disease. Key Words: Seroprevalence, Ducks, Muscouvy Duck, HPAI H5 Subtype
Prototype of A/Duck/Sukoharjo/Bbvw-1428-9/2012 subtipe H5N1 clade 2.3.2 as vaccine on local duck Indriani, Risa; Dharmayanti, NLPI
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 2 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.25 KB) | DOI: 10.14334/jitv.v19i2.1044

Abstract

A/Duck/Sukoharjo/Bbvw-1428-9/2012 virus subtipe H5N1 clade 2.3.2 as seed vaccine on local duck. AI H5N1 clade 2.3.2 vaccine containing 256 HAU per dose was formulated using adjuvant ISA 71VG Montanide ™. Six groups of one day old local duck were used in this study. Three groups (10 ducks per group) were vaccinated and 3 groups (9 duck per group) were served control. Vaccination was conducted when the duck were three weeks old of age using single dose. Three weeks after vaccination when the duck were challenged either with HPAI H5N1 clade 2.3.2, or HPAI H5N1 clade 2.1.3 virus at dose 106 EID50/ 0.1 ml by drops  intranasaly. Result showed that vaccination produced 100% protection compared to unvaccinated ducks againt HPAI subtipe H5N1 clade 2.3.2, and 100% protection againt HPAI H5N1 clade 2.1.3 (A/ck/wj/Subang-29/2007 and A/ck/wj/Smi-Part/2006), while unvaccinated ducks showed virus shedding on day 3 post infection. Key Words: Duck, Influenza, Clade 2.3.2, Vaccine, Clade 2.1.3
Protection of avian influenza (AI) vaccines for poultry against infection of field isolates A/Chicken/West Java/Smi-Pat/2006 and A/Chicken/West Java/Smi-Mae/2008 under laboratory condition Indriani, Risa; Dharmayanti, N.l.p.I.; Adjid, R.M.A.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 2 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.431 KB) | DOI: 10.14334/jitv.v16i2.644

Abstract

The aim of this research was to study level of protection of avian influenza (AI) commercial vaccines available in Indonesia (subtipe H5N1, H5N2 and H5N9) against infection of HPAI field isolates of A/Chicken/West Java/Smi-Pat/2006 and A/Chicken/West Java/Smi-Mae/2008. There were 7 commercial vaccines used in this study, the each vaccines were injected in to 3 weeks old of layer chichickenen intramuscularly. At 3 weeks after vaccination, ten chichickenens from each group were challenged separately with the A/Chicken/West Java/Smi-Pat/2006 and A/Chicken/West Java/Smi-Mae/2008 isolates intranasaly with dose 106 ELD50 per 0,1 ml per chicken. Ten unvaccinated chicken were included in the challenge test as control. The study demonstrate that the AI vaccines with subtipe H5N1 protected chicken (100%) against virus of A/Chicken/West Java/Smi-Pat/2006 and 90-100% against virus A/Chicken/West Java/Smi-Mae/2008. Viral shedding were not seen by 2 days post challenge. The AI vaccines with subtipe H5N2 protected chicken at 20-30% against virus of A/Chicken/West Java/Smi-Pat/2006 and protected chicken at 70-100% against virus of A/Chicken/West Java/Smi-Mae/2008. Viral shedding still detected at 8 days post challenge. The AI vaccines AI with subtipe H5N9 did not protect chicken (0%) against virus A/Chicken/West Java/Smi-Pat/2006 and protected chicken at 50% against virus A/Chicken/West Java/Smi-Mae/2008. Viral shedding still detected by 8 days post challenge. This study concluded that AI vaccines with subtipe H5N1 are better than other AI subtipe vaccines in preventing HPAI virus A/Chicken/West Java/Smi-Pat/2006 dan A/Chicken/West Java/Smi-Mae/2008 infections under laboratory condition. Key Words: Avian Influenza, Vaccine, Poultry, Chicken
Circulating H5N1 virus among native chicken living around commercial layer farms Tarigan, Simson; Indriani, Risa; Ignjatovic, J.
Indonesian Journal of Animal and Veterinary Sciences Vol 20, No 3 (2015): SEPTEMBER 2015
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (510.948 KB) | DOI: 10.14334/jitv.v20i3.1190

Abstract

Soon after the application of vaccination programme against high pathogenic avian influenza H5N1 outbreak of the disease in breeder and commercial layer farms has diminished remarkably in West Java. This study aimed to investigate whether the H5N1 decline is related to the disappearance of source of infection around the farms. Serum samples were collected from 421 native chicken living around commercial layer farms in the Districs of Cianajur and Sukabumi, West Java in March-April 2014.  Antibodies to avian influenza virus (AIV) H5N1 were measured using haemaglutination inhibition (HI), ELISAs and immunoblotting that measured presence of antibodies to the haemagglutin of H5N1 strain, as well as the M2e and nucleoprotein (NP) of all avian influenza viruses. Based on the combined results, 8.6% of the native chickens were seropositive to AI virus based on one or more of serological tests. This study provided serological evidence that H5N1 virus was still circulating among native chicken living around commercial layer farms. Many positive sera were however positive for antibodies in one test only: 2.4%, 3.3% and 3.8% by HI test, M2e and NP ELISA, respectively. It could be speculated that the incongruity of the results is due to the fact that HI, MM2e ELISA and NP ELISA all measure different type of antibodies and the duration of these antibodies in serum following infection with H5N1 differ. The fact that H5N1 virus is still circulating around commercial layer farms infers that the commercial farms are still under threat and therefore vaccination and strict biosecurity are still needed. Key Words: H5N1, Native Chicken, Commercial Layer, Nucleoprotein, M2e, HI Test
Fenotipe Virus Avian Influenza (AI) Subtipe H5N1 Berbeda Karakter Genetik di Indonesia Dharmayanti, NLP Indi; Indriani, Risa; Hartawan, Risza; Ratnawati, Atik
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.3031

Abstract

In Indonesia, data on the phenotype of AI virus subtype H5N1 is very limited, due to the facilities for such testingshould be performed in a laboratory Biosafety level III. Additionally influenza virus has a high error rate duringtranscription of their genome has low RNA polymerase. High error rates generate quasispecies. In this study, wedid some H5N1 viruses infection with different genetic variations and conducted DNA sequencing in severalorgans. To determine the possible emergence detection of quasispecies in different organs and until the virus wasexcreted. We tested the hypothesis that the quasispecies might arise from virus that was infected to theexperimental animals. The results of this study showed that the AI virus subtype H5N1 had different phenotypes inanimal depending on their genetic character. AI virus subtype H5N1 killed chickens within 48-72 hoursdepending on the genetic character of the virus. New AI viruses of subtype H5N1 caused more severe organdamage severe than the character of the old AI viruses. Adaptation of virus in each organ also proved that the viruslikely create variations/quasispecies, which was different from the viral origin.Keywords: Phenotypes, avian influenza, H5N1, genetic character, quasispecies
Efikasi Vaksin Inaktif Bivalen Avian Influenza Virus Subtipe H5N1 (Clade 2.1.3. dan Clade 2.3.2) di Indonesia Dharmayanti, NLP Indi; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1108.821 KB) | DOI: 10.14203/jbi.v11i2.2190

Abstract

ABSTRACTStatus of avian influenza virus subtype H5N1 in Indonesia until 2014 is still endemic in poultry and recorded, there were two types clade of circulating H5N1 namely clade 2.1.3 and the new introduction of lade 2.3.2 since the end of 2012. Both of the clade of avian influenza viruses subtype H5N1 (clade 2.1.3 and 2.3.2) caused the the  AI vaccination program to control of AI in poultry needs to be evaluated.  In this study, we developed a bivalent AI vaccine (which contains clade 2.1.3 and 2.3.2 viruses as a seed vaccine) that   adapted with  the circulation of AI viruses in the field. Result of the study showed that the bivalent vaccine which developed in this study has good efficacy that was challanged with both of AI clade AI and proven to reduce shedding / viral contamination to the environment. It is expected that  the development of bivalent H5N1 vaccine will increase the effectiveness and efficacy of vaccination programs to control highly pathogenic avian influenza disease in Indonesia. Keywords : avian influenza virus, clade, vaccine, bivalent 
Studi Efikasi Vaksin Bivalen AI Isolat Lokal terhadap Beberapa Karakter Genetik Virus AI subtipe H5N1 Indriani, Risa; Dharmayanti, NLP Indi
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.942 KB) | DOI: 10.14203/jbi.v9i1.143

Abstract

Studi vaksin inaktif bivalen AI isolat lokal subtipe H5N1 terhadap beberapa karakter genetik virus AI H5N1 padaayam layer dan broiler. Vaksin inaktif bivalen dari isolat lokal AI A/Ck/west java/Smi-M6/2008 and A/Ck/westjava/Pwt-D10-39/2010. Ayam layer dan broiler komersial divaksinasi dengan vaksin inaktif bivalen AI isolat lokal,setelah 3 minggu vaksinasi ditantang dengan virus AI A/Ck/west java/Smi-Part/2006, A/Ck/west java/Subang-JAPFA-29/2007 and A /Ck/west java/Smi-Rahm2/2011. Ayam layer vaksinasi mendapat perlindungan dari morbiditas,mortalitas dan penurunan ekskresi virus tantang dengan tingkat proteksi 90-100% sedangkan ayam layerkontrol mati dalam waktu 2-3 hari, sementara broiler yang divaksinasi tidak mendapatkan perlindungan dari morbiditasdan mortalitas setelah terinfeksi virus AI tantang. Hasil studi memperlihatkan vaksin inaktif bivalen AI isolatlokal subtipe H5N1 mampu memberikan perlindungan pada ayam layer dari infeksi beberapa karakter genetikvirus AI subtipe H5N1 .Kata kunci: Vaksin bivalen, subtipe H5N1, tantang dan proteksi
Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Antibody in Chickens Using Local Isolate of PTS III Indriani, Risa; Indi Dharmayanti, NLP.
JURNAL BIOLOGI INDONESIA Vol 9, No 2 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (164.167 KB) | DOI: 10.14203/jbi.v9i2.162

Abstract

An indirect enzyme-linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectiousbronchitis (IBV). Antigen was prepared from whole virus of infectious bronchitis local isolate PTS-III serotype.Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera indeveloped ELISA were determined 0.4μg/well, 1:2000 and 1:100, respectively. Correlation optical densities (OD)were compared with a standard commercial ELISA (R2=0.933). The developed ELISA has a better sensitivity to hemagglutinationinhibition (HI) test. The developed local isolate ELISA can be used to detect antibody against infectiousbronchitis virus and it is suitable for sample screening at the diagnostic laboratories.Keywords: ELISA, antibody, chicken, IB PTS-III local isolate
Respon Titer Antibodi dan Proteksi Virus Newcastle Disease Genotype I, II, VI dan VII Sebagai Vaksin Terhadap Infeksi Isolat Virus Newcastle Disease Chicken/Indonesia/GTT/11. Indriani, Risa; Dharmayanti, NLP Indi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1354.779 KB) | DOI: 10.14203/jbi.v12i2.2887

Abstract

ABSTRACTThe Newcastle Disease (ND) virus causes the most important disease in chicken, and very contagiuos in poultry in many countries. In Indonesia the Newcastle Disease or is called tetelo became endemic in chicken currently, the ND virus of genotype VII caused morbidity 100% and mortality 80% in chicken. The fusion and haemaglutinin-neuraminidase protein are involved in adhesion to the surface of hert cells to antibodies. HN and F protein induced by the ND vaccines have neutralizing effects. The aim of this study was to compare the antibody level and protection of several ND vaccine genotypes (genotype I, II, VI and VII) in chicken against ND virus chicken / Indonesia/GTT/11 genotype VII. Our result showed that all genotype ND vaccines (I, II, VI dan VII) produce 100% protection in chicken from clinical and mortality, but the challenge virus excretion in chickens vaccinated with ND vaccine VII genotypes were significantly different (p <0.05) to other groups. Response of antibody in chicken vaccinated ND genotype VII show highest mean titer (GMT 122) compered other ND vaccine. In onclusion, the ND virus chicken/Indonesia/ GTT/11 genotype VII is good to be used as inactive vaccine seed, which appropriate to the field virus circulating at this time.Keywords: virus, Newcastle Disease, genotype, vaccine
Sirkulasi virus Avian influenza H5N1 Tahun 2010 : Virus genetic drift mirip A/Ck/West Java/Pwt-Wij/2006 ditemukan di beberapa kabupaten di Sumatra dan Jawa Dharmayanti, NLP Indi; Ratnawati, Atik; Hewajuli, Dyah Ayu; Indriani, Risa
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2654.808 KB) | DOI: 10.14203/jbi.v8i1.3069

Abstract

The avian influenza H5N1 virus circulation in 2010 : Genetic Drift Like Virus A/Chicken/West Java/Pwt-Wij/2006 was found in several districts of Sumatra and Java. Until 2011, the H5N1 subtype of AI virus is still circulating in many parts of Indonesia. The discovery of the AI viruses which have undergone genetic drift since 2006 until now requires serious attention from the government in terms of AI disease control, the surveillance and monitoring of virus circulation and execution of genetic mapping to determine the genetic character of the AI virus at the molecular level, especially on the surface of glycoproteins (HA and NA protein).This information is needed to determine the diversity and character of the AI virus in Indonesia. Genetic data are used to evaluate the strategy to control AI in Indonesia, such as vaccination and the vaccine seed used and determine the extent of AI virus mutation in Indonesia has beenmutated. This study conducted by monitoring of the AI virus circulation throughout 2010. The methods used were AI virus isolation, RT-PCR, sequencing of genes coding for viral surface and the prediction of three-dimensional analysis to determine the location of virusmutation. The results of this study showed that most of the AI virus subtype H5N1, which was isolated during the year 2010, showed similar mutations to the genetic drift virus in 2006, A /Ck/West Java/Pwt-Wij/2006. The viruses were characterized by the presence of 18-19 amino acid substitutions at the level of the HA protein. On the NA protein level, there is a single mutation which was buried in the NA molecule. This mutation probably did not influence for NA activity. Genetic mapping of AI virus subtype H5N1 in 2010 showed that the viral genetic driftas the mutan virus A/Ck/West Java/Pwt-Wij/2006 have circulated not only in West Jawa alone but has been found on the island of Sumatra, Banten, West Jawa and East Jawa.Keywords: Circulation, avian influenza H5N1 virus, genetic drift
Co-Authors A Wiyono Ahpas, Ahpas Atik Ratnawati Bambang Ngaji Utomo Darminto . Dewiyanti, Rina Dyah Ayu Hewajuli E. Martindah Eny Martindah Fairusya, Nuha Farida Syamsiah, Farida Fitrine Ekawasti, Fitrine Helmy Hamid Hoerudin, Heri Indi Dharmayanti Indi Dharmayanti, NLP. Indi Dharmayanti, NLP. Izzah, Ukhtul J. Ignjatovic, J. Jejen Jaelani L Parede Lies Parede Muharam Saepulloh N.L.P Indi Dharmayanti N.L.P.I Dharmayanti N.L.P.I. Dharmayanti NI LUH PUTU INDI DHARMAYANTI NLP I Dharmayanti NLP Indi Dharmayanti NLP. Indi Dharmayanti, NLP. Indi NLPI Dharmayanti Nuradji, Harimurti Putri, Ajeng Fabeana R.M Abdul Adjid R.M.A. Adjid Rahmat Setya Adji RISZA HARTAWAN S Wahyuwardani sekarmila, Gita Simson Tarigan Sukarsih . Supriyanto Supriyanto Suryatmiati, Sri Trianita, Desi Ukhti Dwi Rillah, Ukhti Dwi Wawan Gunawan Winarsongko, Agus yuda pratama