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All Journal Jurnal Penelitian Hutan Tanaman JURNAL PEMULIAAN TANAMAN HUTAN Indonesian Journal of Biotechnology Agrienvi : Jurnal Ilmu Pertanian Jurnal Wana Tropika Jurnal Ilmu Kehutanan Jurnal Ilmu Kehutanan Jurnal Wana Tropika

Sapto Indrioko

Faculty of Forestry, Gadjah Mada University, Yogyakarta, Indonesia

Author-ID : 287403

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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Articles

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Paternity Analysis of Tea (Camellia sinensis L. Kuntz) Hybrids Using Isozyme Marker Setyorini, Titin; ., Taryono; ., Suyadi; Indrioko, Sapto
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

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Tea plant has been categorized as self-incompatible crop. This is the reason behind the high genetic diversity. Natural pollination is possible to occur and the male parent is usually unknown, therefore, there is a need of method to identify male parent of hybrids through paternity analysis. Isozyme markers have been successfully used for paternity analysis due to their co-dominant polymorphism. This research aimed to predict male parents of hybrids by figuring out the mating system through isozyme banding patterns. In this experiment, seven enzyme systems were evaluated, of which only two of the enzyme systems i.e. esterase and shikimate dehydrogenase showing clear band pattern of Est-1, Est-2, and Shd-1 loci. The mating system of tea could be categorized as a mixed mating model, with high estimated out-crossing rate of 98.6 %. The pollen contributors were not always originated from the vicinity of the female parents.Key words: isozyme markers, paternity analysis, tea

Allozyme variation of the endemic and vulnerable Dyera lowii Hook.f. in Central Kalimantan: Implications for genetic resources conservation Wahyudiningsih, Tri Suwarni; Naiem, Mohammad; Indrioko, Sapto; Sumardi, Issirep
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

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Dyera lowii is an endemic and vulnerable tree species of commercial value as chewing gum found inpeat swamp forests, scatteredly distributed in Sumatra, Kalimantan, and Peninsular Malaysia. Their existenceis now under severe threat due to habitat conversion. This study is aimed to assess genetic diversity withinfour natural populations (Hampangen, Parahangan, Sebangau, Selat Nusa ) and one plantation in CentralKalimantan based on allozyme variation. Electrophoresis procedures were conducted with an isoelectricfocusing polyacrylamide slab gel system. The result showed high genetic diversity (HE=0.52) and gene fl ow(3.402) seemed to be effective. A total of 14 alleles were found among all the analysed population. Meannumber of alleles per locus (Aa) was 3.206, and the effective number of alleles per locus (Ae) was 2.21. Geneticdifferentiation between populations (FST) was signifi cant at the moderately level (0.0685). Most allozymevariation was found within population (93.2%). Special attention is essential to conserve a private allele ofGot-1-e (9%) at Selat Nusa population. Sebangau population missed the alleles of Est-2-b and Got-1-a, as foundin other populations. Selat Nusa population is expected to enhance the effective management for geneticresources conservation of this vulnerable species in the future.

In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection Asri Insiana Putri; Mohammad Na'iem; Sapto Indrioko; Sri Rahayu; Ari Indrianto
Indonesian Journal of Biotechnology Vol 19, No 2 (2014)
Publisher : Universitas Gadjah Mada

In vitro screening of Falcataria moluccana (Miq.) was conducted by tissue culture method. Seeds fromtwo different site of community forest, 400 m (S1) and 800 m (S2) above sea level, were used as material.Double concentration of MS (Murashige & Skoog, 1962) with 40 mg/l gall rust (Uromycladium tepperianum(Sacc.) fi ltrate were used for media selection. The results of this research showed that 66 % axenic plantlets invitro from S1 and 27 % from S2 were still survived after 3 months incubation without subculture. The meanof fresh weight (2. 21 ± 0. 26 g) and dry weight (1. 97 ± 0. 12 g) from S1 plantlets lower than the mean of freshweight (2. 87 ± 0,18 g) and dry weight (2. 16 ± 0. 14 g) from S2 plantlets. Qualitative of terpenes, saponins andquantitative of total phenolics were analyzed from those gall rust extract, as source of fi ltrate media, attackedand un-attacked of F. moluccana. They all qualitatively have capability to produce terpenoid and saponin. Itis notice that U. tepperianum, un-cultured pathogen, contain of those compound that may play a role as codeterminantsof pathogenecity. While the highest total phenolic compound were contained in gall rust extract(2. 35 %), followed by attacked F. moluccana branches (1. 18 %) and un-attacked F. moluccana branches (0. 44%). This indicated that phenolic compound in gall rust has higher activity as a response of F. moluccana to U.tepperianum pathogen pressures and result of this study suggest the great value of gall rust fi ltrate for use asmedia selection in vitro.

Somatic embryogenesis of Sandalwood (Santalum album L.) Toni Herawan; Mohammad Na'iem; Sapto Indrioko; Ari Indrianto
Indonesian Journal of Biotechnology Vol 19, No 2 (2014)
Publisher : Universitas Gadjah Mada

Sandalwood (Santalum album L.) is native species of Indonesia, especially in East Nusa Tenggara, is oneof the twenty two species of the genus Santalum in the world. Sandalwood is an important tree because it hashigh economic value can produce sandal oil these can be used for perfumes, cosmetics, pharmaceuticals, andare often used in religious ceremonies. In vitro particularly somatic embryogenesis has been widely appliedin the propagation of sandalwood. The Objective of this research is to obtain regeneration of sandalwoodthrough somatic embryogenesis using leaves explant from various clones. Medium for embryo induction is MS(Murashige and Skoog, 1962) solid medium containing treatment of 2,4-D (2,4-Dichlorophenoxyacetic acid)at various concentrations. To the media 0,15 mg /l kinetin, 40 g/l sucrose, and 2,5 g/l gelrite were added.Culture were incubated in the dark. Medium for Embryo development (maturation) is MS solid mediumcontaining treatment of BAP (Benzyl-amino-purine) at various concentrations. To the media 0,01 mg /l NAA(Napthalene-acetic-acid), 40 g/l sucrose, and 2,5 g/l gelrite were added. Culture were incubated in the light. Tostudy the specifi c structure of sandalwood somatic embryo early detection was conducted using histologicalanalysis. Results of anova showed that the clones, media, and interaction between clones with media did notsignifi cantly affect the development of sandalwood callus percentage. Results of anova showed that the clonesand BAP concentration signifi cantly effect to the embryo development of sandalwood.

The effects of population size on genetic parameters and mating system of sandalwood in Gunung Sewu, Indonesia Yeni Widyana Nurchahyani; Sapto Indrioko; Eny Faridah; Atus Syahbudin
Indonesian Journal of Biotechnology Vol 20, No 2 (2015)
Publisher : Universitas Gadjah Mada

We combined feld observations with isoenzyme analysis to compare population demographic and its effects on genetic diversity and mating systems, among six populations of sandalwood in Gunung Sewu, Indonesia, during March to August 2015. This endangered economic-important species was originated from the southeastern parts of Indonesia, but is recently occured as new landraces in Gunung Sewu, Java island. The observed heterozygosity varied from Ho 0.184 to 0.385 in parents, and from Ho 0.083 to 0.348 in offspring levels, based on the degree of clonality and genetic base. Most of genetic variation is distributed within populations, and only 2.7% were presented among populations, that was indicated by the low DST and FST value (HT 0.30; HS 0.276; DST 2.4%; FST 7.98%). A dendrogram indicated a grouping of populations into three clusters. However, there were seemed to be no association between geographical and genetic distance. Genetic depletion occured due to (i) clonality events as result of heavy-exploitation and/or natural disturbance which induced root suckering, (ii) genetic drifts and bottleneck effects, (iii) the founder effects due to parental low diversity, and (iv) the alteration on mating systems to be more inbreeders. Some of the results confrmed a “reproductive assurance prediction” while some others were contradicting this. It seemed that genetic diversity and mating systems are not much affected by population size, but more by the parental heterozygosity and the degree of clonality. Our results emphasized the importance of populations’ genetic base or parental genetic diversity to naturally maintain the genetic and evolutionary processes under equilibrium conditions.

Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers Jayusman Jayusman; Muhammad Na’iem; Sapto Indrioko; Eko Bhakti Hardiyanto; ILG Nurcahyaningsih
Indonesian Journal of Biotechnology Vol 22, No 1 (2017)
Publisher : Universitas Gadjah Mada

Surian Toona sinensis Roem is one of the most widely planted species in Indonesia. This study aimed to estimate the genetic diversity between a number of surian populations in a progeny test using RAPD markers, with the goal of proposing management strategies for a surian breeding program. Ninety-six individual trees from 8 populations of surian were chosen as samples for analysis. Eleven polymorphic primers (OP-B3, OP-B4, OP-B10, OP-H3, OP-Y6, OP-Y7, OP-Y8, OP-Y10, OP-Y11, OP-Y14, and OP-06) producing reproducible bands were analyzed for the 96 trees, with six trees per family sampled. Data were analyzed using GenAlEx 6.3, NTSYS 2.02. The observed percentage of polymorphic loci ranged from 18.2% to 50%. The mean level of genetic diversity among the surian populations was considered to be moderate (He 0.304). Cluster analysis grouped the genotypes into two main clusters, at similarity levels of 0.68 and 0.46. The first two axes of the PCoA explained 46.16% and 25.54% of the total variation, respectively. The grouping of samples into clusters and subclusters did not correspond with family and their distances, but the grouping was in line with the genetic distances of the samples.

Co-Authors Aan Yuli Widhianto Achmad Ramadan Agus Sofyan Ari Indrianto Ari Indrianto Arif Nirsatmanto Aris Wibowo Asri Insiana Putri Atus Syahbudin Aziz Akbar M Bangun Baramantya Budiad Budiad Budiadi Budiadi Daryono Prehaten Deanova Frestiana Br Pelawi Eko Bhakti Hardiyanto Eko Bhakti Hardiyanto Eko Bhakti Hardiyanto Eny Faridah Farobi, Ibnu Fasis Mangkuwibowo Haryono Supriyo Hidayati, Fanny ILG Nurcahyaningsih Issirep Sumardi Jayusman Jayusman Julianus Kinho Lies Rahayu Wijayanti Faida Lutfiana, Eva Mohammad Na'iem Mohammad Na'iem Mohammad Na'iem Mohammad Na'iem Mohammad Naiem Muhammad Fadhil Amiruddin Sudomo Muhammad Na’iem Na'iem, Mohammad Novita Anggraini R. Sapto Hendri Boedi Soesatyo Ridla Arifriana Rini Purwiastuti Sabarnurrdin, Sambas SRI RAHAYU Sri Rahayu Suryo Hardiwinoto Suryo Hardiwinoto Suyadi . Titin Setyorini, Titin Toni Herawan Toni Herawan Tri Suwarni Wahyudiningsih Tuharno Tuharno Wiwik Handayani Yayan Hadiyan Yeni Widyana Nurcahyani Cahyaningrum Yeni Widyana Nurchahyani

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