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Allozyme variation of the endemic and vulnerable Dyera lowii Hook.f. in Central Kalimantan: Implications for genetic resources conservation Wahyudiningsih, Tri Suwarni; Naiem, Mohammad; Indrioko, Sapto; Sumardi, Issirep
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.811 KB)

Dyera lowii is an endemic and vulnerable tree species of commercial value as chewing gum found inpeat swamp forests, scatteredly distributed in Sumatra, Kalimantan, and Peninsular Malaysia. Their existenceis now under severe threat due to habitat conversion. This study is aimed to assess genetic diversity withinfour natural populations (Hampangen, Parahangan, Sebangau, Selat Nusa ) and one plantation in CentralKalimantan based on allozyme variation. Electrophoresis procedures were conducted with an isoelectricfocusing polyacrylamide slab gel system. The result showed high genetic diversity (HE=0.52) and gene fl ow(3.402) seemed to be effective. A total of 14 alleles were found among all the analysed population. Meannumber of alleles per locus (Aa) was 3.206, and the effective number of alleles per locus (Ae) was 2.21. Geneticdifferentiation between populations (FST) was signifi cant at the moderately level (0.0685). Most allozymevariation was found within population (93.2%). Special attention is essential to conserve a private allele ofGot-1-e (9%) at Selat Nusa population. Sebangau population missed the alleles of Est-2-b and Got-1-a, as foundin other populations. Selat Nusa population is expected to enhance the effective management for geneticresources conservation of this vulnerable species in the future.

Allozyme variation of the endemic and vulnerable Dyera lowii Hook.f. in Central Kalimantan: Implications for genetic resources conservation Tri Suwarni Wahyudiningsih; Mohammad Naiem; Sapto Indrioko; Issirep Sumardi
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Dyera lowii is an endemic and vulnerable tree species of commercial value as chewing gum found inpeat swamp forests, scatteredly distributed in Sumatra, Kalimantan, and Peninsular Malaysia. Their existenceis now under severe threat due to habitat conversion. This study is aimed to assess genetic diversity withinfour natural populations (Hampangen, Parahangan, Sebangau, Selat Nusa ) and one plantation in CentralKalimantan based on allozyme variation. Electrophoresis procedures were conducted with an isoelectricfocusing polyacrylamide slab gel system. The result showed high genetic diversity (HE=0.52) and gene fl ow(3.402) seemed to be effective. A total of 14 alleles were found among all the analysed population. Meannumber of alleles per locus (Aa) was 3.206, and the effective number of alleles per locus (Ae) was 2.21. Geneticdifferentiation between populations (FST) was signifi cant at the moderately level (0.0685). Most allozymevariation was found within population (93.2%). Special attention is essential to conserve a private allele ofGot-1-e (9%) at Selat Nusa population. Sebangau population missed the alleles of Est-2-b and Got-1-a, as foundin other populations. Selat Nusa population is expected to enhance the effective management for geneticresources conservation of this vulnerable species in the future.

Morphological Characteristics and Similarity Analysis of Cassava (Manihot esculenta Crantz) in Wonosobo, Temanggung, and Magelang Regencies Sugiyarto Sugiyarto; Tri Suwarni Wahyudiningsih; Ericka Darmawan; Listya Hidayah
Jurnal Biodjati Vol 8, No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.25660

The diversity of cassava in Wonosobo, Temanggung and Magelang districts must be investigated. The objective of the study was to analyze the diversity of cassava based on morphological characteristics. The cassava of each accession was planted in Bandongan, Magelang Regency. Morphological characters observed after six months of planting included apical leaf color, apical leaf hair, leaf lobe shape, petiole color, leaf color, number of leaf lobes, leaf length, leaf width, petiole length, vein color, petiole orientation, color epidermis of stem, color of outer appearance of stem, distance between nodes, stem growth, color of mature branch tip, branching type, tuber, tuber shape, outer tuber skin color, tuber flesh color, and tuber inner skin color. Analysis of the morphological characteristics of cassava is presented in the form of a dendrogram using the MVSP (Multi Variate Statistical Package) version 3.22. The results of the study obtained 39 accessions of cassava which showed different morphological characteristics. The lowest morphological similarity (0.735) was found in Group I only consisted of 2 acquisitions that did not have tubers, namely Red Vegetables and Green Vegetables and Group II ( Groups III and IV) have a similarity of 0.92. The highest similarity was in groups IIIa and IIIb and Groups IVa and IVb have a similarity coefficient value of 0.95. The morphological diversity of 39 accessions is a source of cassava germplasm: 37 accessions are harvested for tubers and 2 accessions areharvested explicitly for leaves as vegetables. This research was useful for the development of cassava-based food industry bioproducts.

RANCANG BANGUN KERANGKA AKUAPONIK MODEL KINCIR UNTUK BUDIDAYA TANAMAN SAYURAN Yuliantari, Risky Via; Hilmy, Fuad; Wahyudiningsih, Tri Suwarni; Iftitah, Siti Nurul; Jannah, Eka Nur; Fatikhin, Choirul; Sholeh, Muhamad Khoirul; Setyawan, Muhammad Riko; Setiyawan, Rizky Joko
Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Vol 6, No 3 (2023): Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstormin
Publisher : Politeknik Harapan Bersama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30591/japhb.v6i3.4608

Akuaponik memiliki prinsip kerja dengan mengalirkan air dari kolam ikan ketempat media tanam. Kemudian air akan kembali mengalir dalam kolam, begitu seterusnya. Air yang mengalir dari dalam kolam ikan yang kotor dan kaya unsur hara akan diserap oleh akar tanaman. Sistem tanam akuaponik ada beberapa jenis, diantaranya adalah Nutrient Film Techniquw (NFT) yang merupakan sistem budidaya dengan meletakkan akar tanaman pada lapisan air yang dangkal, Media Filled Beds (MFB) menggunakan kontainer diisi dengan media batu dari tanah liat, dan Deep Water Culture (DWC) sistem akuaponik dengan tanaman yang mengambang diatas air. Jenis system tanam akuaponik tersebut memiliki perbedaan mekanisme dengan kontruksi yang berbeda dan memberikan hasil panen yang berbeda pula. Masyarakat Desa Gunungpring memanfaatkan sumber daya air yang baik hanya untuk memelihara berbagai jenis ikan dalam kolam. Padahal kolam tersebut berpotensi digunakan sebagai budidaya tanaman sayuran yang bermanfaat untuk keluarga dan bisa menambah pemasukan ekonomi. Oleh kerana itu dilakukan inovasi dalam penerapan budidaya tanaman sayuran dengan akuaponik model kincir yang bisa menghemat air, menghemat lahan, serta memberikan sirkulasi pada air dalam kolam dan tanaman. Selain itu, akuaponik model kincir dapat menambah nilai estetika dan kreativitas dibidang pertanian.

Ethnobotany of Taro Plant Diversity (Colocasia esculenta L. Schott) in Magelang City and Regency as a Biological Reference Book Wakhidatunnisa, Yunika Nurma; Kusumaningrum, Serafica Btari Christiyani; Darmawan, Ericka; Wahyudiningsih, Tri Suwarni
Biosfer: Jurnal Pendidikan Biologi Vol. 18 No. 1 (2025): Biosfer: Jurnal Pendidikan Biologi
Publisher : Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/biosferjpb.47491

The diversity of taro plants in the Magelang area has been used for a long time by traditional communities from generation to generation. But knowledge related to the use of taro plants for purposes other than food is not widely known to the public. This study aims to determine the untilization of taro plants (Colocasia esculenta L. Schott) and analyze the feasibility of ethnobotanical reference books of taro plant diversity (Colocasia esculenta L. Schott) in Magelang City and Regency. This study is a type of mixed method research, which is a combination of ethnographic research methods with Research and Development (R&D) research. The results of ethnographic research are then used as a source of reading through the development of reference books. Development research was carried out using a 4-D research model and then limited to 3-D (define, design, and develop) due to time constraints. Based on the research that has been carried out, it is known that there are 5 varieties of taro plants (Colocasia esculenta L. Schott) that are found and used by the local people of Magelang, namely Taro Pratama 1, Pratama 2, Pratama 3, Taro Water/Bogor, and black Taro which are used by the community for various life purposes. The reference book based on the ethnobotanical study of taro plant diversity developed is suitable for use as a biological reference book with a percentage of 91% in material experts with excellent category, 83% in media experts with good category, and 95.89% in the results of community response tests with excellent category.

Konservasi Coelogyne pandurata Lindh. di Kalimantan Tengah: Karakter Morfologi, Propagasi In Vitro, dan Pelestarian Berbasis Komunitas Lokal Tri Suwarni Wahyudiningsih; Y Jagau; N Ravenska
Jurnal Pengelolaan Lingkungan Berkelanjutan (Journal of Environmental Sustainability Management) JPLB, Vol 2, No 2 (2018)
Publisher : Badan Kerjasama Pusat Studi Lingkungan (BKPSL) se-Indonesia bekerjasama dengan Pusat Penelitian Lingkungan Hidup IPB (PPLH-IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36813/jplb.2.2.125-139

C. pandurata merupakan anggrek endemik, termasuk Appendiks I menurut CITES, dan mempunyai nilai ekonomi tinggi. Kebakaran hutan, konversi lahan, eksploitasi tidak terkendali, periode berbunga sangat pendek, dan sulit disilangkan menjadi faktor penyebab yang membuat populasi C pandurata terancam punah sehingga perlu dilakukan konservasi. Penelitian ini bertujuan untuk mengkaji perbedaan karakter morfologi C. pandurata dari Muara Teweh dan Tewah, propagasi secara in vitro, serta pelestarian berbasis komunitas masyarakat lokal. Bunga asal Tewah memiliki ciri-ciri seperti warna lidah lebih hitam, bulu halus dan ornamen lebih jelas, panjang dan lebar pada bulbus dan daun asal Tewah lebih besar. Eksplan serbuk biji ditanam pada medium perlakuan: I. MS (kontrol), II. MS + 3 mg/l BAP (Benzyl Amino Purine), III. MS + 3 mg/l BAP + Pisang, IV. MS + 3 mg/l BAP + air kelapa, dan V. MS + 3 mg/l BAP + tomat. Pada 10 HST (Hari Setelah Tanam) terjadi perubahan warna biji kuning menjadi hijau. Pro-meristem mengalami diferensiasi menjadi kutub calon akar pada suspensor dan kutub calon tunas. Protocorm tumbuh pada 21 HST. Seedling tumbuh pada 8 MST (Minggu Setelah Tanam) pada perlakuan I, II, dan IV. Pada perlakuan III seedling dengan tunas 0,5 cm terlihat pada 9 MST. Pada perlakuan V seedling tumbuh pada 11 MST dengan perawakan sehat dan kuat. Komunitas masyarakat lokal dan hutan adat menjadi prioritas awal pembinaan dan pemberdayaan melalui kegiatan pengenalan anggrek, teknik budi daya, pemahaman konservasi serta penanaman ke habitat, sehingga konservasi in-situ dapat berjalan secara berkesinambungan.

Analisis Keragaman Genetik Plasma Nutfah Tanaman Kayu Putih (Melaleuca cajuputi subsp. cajuputi) Berdasarkan Karakter Morfologi dan Anatomi Daun serta Oil Glands Aswin Hendry Atmoko; Tri Suwarni Wahyudiningsih; Noor Khomsah Kartikawati
JURNAL TRITON Vol 14 No 2 (2023): JURNAL TRITON
Publisher : Politeknik Pembangunan Pertanian Manokwari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47687/jt.v14i2.699

Tanaman kayu putih merupakan tanaman hutan asli Indonesia yang dimanfaatkan untuk produksi minyak atsiri. Keragaman genetik tanaman kayu putih yang tersebar di zona persebaran alami cukup beragam. Diperlukan adanya studi dan penelitian untuk mempelajari hubungan kekerabatan antar tanaman kayu putih dan keragaman genetik plasma nutfah tanaman kayu putih. Studi kekerabatan dan juga keragaman genetik plasma nutfah didasarkan pada kemiripan karakter fenetik (kuantitatif dan kualitatif) morfologi dan anatomi daun. Terdapat 36 klon unggul tanaman kayu putih yang dilakukan pengamatan morfologi dan anatomi daun untuk menentukan dendogram. Penelitian yang dilakukan merupakan penelitian deksriptif dan juga penelitian eksperimental yang disusun dalam Rancangan Acak Lengkap (RAL) non faktorial dengan faktor yaitu macam klon yang terdiri atas 36 taraf (macam klon) dan dilakukan analisis sidik ragam menggunakan ANOVA dan dilakukan uji lanjut menggunakan uji Duncan’s. Dendogram dibuat menggunakan aplikasi MVSP versi 3.22. Hasil penelitian menunjukkan bahwa keragaman genetik yang luas pada 36 klon unggul tanaman kayu putih. Pada dendogram terbentuk 4 klaster dengan 8 klon yang menunjukkan tingkat kemiripan yang tinggi berdasarkan nilai disimilaritas sebesar 0,0. klon 14 dan 36 merupakan klon dengan rerata jumlah kelenjar minyak tertinggi. Berdasarkan penelitian yang dilakukan dapat dipahami bahwa keragaman genetik pada 36 klon unggul tanaman kayu putih cukup tinggi, hal tersebut mendukung upaya pemuliaan tanaman karena database yang diperoleh memberikan informasi klon yang dapat digunakan sebagai indukan untuk meningkatkan kemampuan produksi minyak kayu putih karena memiliki potensi produksi minyak yang tinggi.

Morphological Characteristics and Similarity Analysis of Cassava (Manihot esculenta Crantz) in Wonosobo, Temanggung, and Magelang Regencies Sugiyarto, Sugiyarto; Wahyudiningsih, Tri Suwarni; Darmawan, Ericka; Hidayah, Listya
Jurnal Biodjati Vol 8 No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.25660

The diversity of cassava in Wonosobo, Temanggung and Magelang districts must be investigated. The objective of the study was to analyze the diversity of cassava based on morphological characteristics. The cassava of each accession was planted in Bandongan, Magelang Regency. Morphological characters observed after six months of planting included apical leaf color, apical leaf hair, leaf lobe shape, petiole color, leaf color, number of leaf lobes, leaf length, leaf width, petiole length, vein color, petiole orientation, color epidermis of stem, color of outer appearance of stem, distance between nodes, stem growth, color of mature branch tip, branching type, tuber, tuber shape, outer tuber skin color, tuber flesh color, and tuber inner skin color. Analysis of the morphological characteristics of cassava is presented in the form of a dendrogram using the MVSP (Multi Variate Statistical Package) version 3.22. The results of the study obtained 39 accessions of cassava which showed different morphological characteristics. The lowest morphological similarity (0.735) was found in Group I only consisted of 2 acquisitions that did not have tubers, namely Red Vegetables and Green Vegetables and Group II ( Groups III and IV) have a similarity of 0.92. The highest similarity was in groups IIIa and IIIb and Groups IVa and IVb have a similarity coefficient value of 0.95. The morphological diversity of 39 accessions is a source of cassava germplasm: 37 accessions are harvested for tubers and 2 accessions areharvested explicitly for leaves as vegetables. This research was useful for the development of cassava-based food industry bioproducts.

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