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Allozyme variation of the endemic and vulnerable Dyera lowii Hook.f. in Central Kalimantan: Implications for genetic resources conservation Wahyudiningsih, Tri Suwarni; Naiem, Mohammad; Indrioko, Sapto; Sumardi, Issirep
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.811 KB)

Abstract

Dyera lowii is an endemic and vulnerable tree species of commercial value as chewing gum found inpeat swamp forests, scatteredly distributed in Sumatra, Kalimantan, and Peninsular Malaysia. Their existenceis now under severe threat due to habitat conversion. This study is aimed to assess genetic diversity withinfour natural populations (Hampangen, Parahangan, Sebangau, Selat Nusa ) and one plantation in CentralKalimantan based on allozyme variation. Electrophoresis procedures were conducted with an isoelectricfocusing polyacrylamide slab gel system. The result showed high genetic diversity (HE=0.52) and gene fl ow(3.402) seemed to be effective. A total of 14 alleles were found among all the analysed population. Meannumber of alleles per locus (Aa) was 3.206, and the effective number of alleles per locus (Ae) was 2.21. Geneticdifferentiation between populations (FST) was signifi cant at the moderately level (0.0685). Most allozymevariation was found within population (93.2%). Special attention is essential to conserve a private allele ofGot-1-e (9%) at Selat Nusa population. Sebangau population missed the alleles of Est-2-b and Got-1-a, as foundin other populations. Selat Nusa population is expected to enhance the effective management for geneticresources conservation of this vulnerable species in the future.
Allozyme variation of the endemic and vulnerable Dyera lowii Hook.f. in Central Kalimantan: Implications for genetic resources conservation Tri Suwarni Wahyudiningsih; Mohammad Naiem; Sapto Indrioko; Issirep Sumardi
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (500.811 KB) | DOI: 10.22146/ijbiotech.8637

Abstract

Dyera lowii is an endemic and vulnerable tree species of commercial value as chewing gum found inpeat swamp forests, scatteredly distributed in Sumatra, Kalimantan, and Peninsular Malaysia. Their existenceis now under severe threat due to habitat conversion. This study is aimed to assess genetic diversity withinfour natural populations (Hampangen, Parahangan, Sebangau, Selat Nusa ) and one plantation in CentralKalimantan based on allozyme variation. Electrophoresis procedures were conducted with an isoelectricfocusing polyacrylamide slab gel system. The result showed high genetic diversity (HE=0.52) and gene fl ow(3.402) seemed to be effective. A total of 14 alleles were found among all the analysed population. Meannumber of alleles per locus (Aa) was 3.206, and the effective number of alleles per locus (Ae) was 2.21. Geneticdifferentiation between populations (FST) was signifi cant at the moderately level (0.0685). Most allozymevariation was found within population (93.2%). Special attention is essential to conserve a private allele ofGot-1-e (9%) at Selat Nusa population. Sebangau population missed the alleles of Est-2-b and Got-1-a, as foundin other populations. Selat Nusa population is expected to enhance the effective management for geneticresources conservation of this vulnerable species in the future.
Morphological Characteristics and Similarity Analysis of Cassava (Manihot esculenta Crantz) in Wonosobo, Temanggung, and Magelang Regencies Sugiyarto Sugiyarto; Tri Suwarni Wahyudiningsih; Ericka Darmawan; Listya Hidayah
Jurnal Biodjati Vol 8, No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.25660

Abstract

The diversity of cassava in Wonosobo, Temanggung and Magelang districts must be investigated. The objective of the study was to analyze the diversity of cassava based on morphological characteristics. The cassava of each accession was planted in Bandongan, Magelang Regency. Morphological characters observed after six months of planting included apical leaf color, apical leaf hair, leaf lobe shape, petiole color, leaf color, number of leaf lobes, leaf length, leaf width, petiole length, vein color, petiole orientation, color epidermis of stem, color of outer appearance of stem, distance between nodes, stem growth, color of mature branch tip, branching type, tuber, tuber shape, outer tuber skin color, tuber flesh color, and tuber inner skin color. Analysis of the morphological characteristics of cassava is presented in the form of a dendrogram using the MVSP (Multi Variate Statistical Package) version 3.22. The results of the study obtained 39 accessions of cassava which showed different morphological characteristics. The lowest morphological similarity (0.735) was found in Group I only consisted of 2 acquisitions that did not have tubers, namely Red Vegetables and Green Vegetables and Group II ( Groups III and IV) have a similarity of 0.92. The highest similarity was in groups IIIa and IIIb and Groups IVa and IVb have a similarity coefficient value of 0.95. The morphological diversity of 39 accessions is a source of cassava germplasm: 37 accessions are harvested for tubers and 2 accessions areharvested explicitly for leaves as vegetables. This research was useful for the development of cassava-based food industry bioproducts.
RANCANG BANGUN KERANGKA AKUAPONIK MODEL KINCIR UNTUK BUDIDAYA TANAMAN SAYURAN Yuliantari, Risky Via; Hilmy, Fuad; Wahyudiningsih, Tri Suwarni; Iftitah, Siti Nurul; Jannah, Eka Nur; Fatikhin, Choirul; Sholeh, Muhamad Khoirul; Setyawan, Muhammad Riko; Setiyawan, Rizky Joko
Jurnal Pengabdian Masyarakat Progresif Humanis Brainstorming Vol 6, No 3 (2023): Jurnal Abdimas PHB : Jurnal Pengabdian Masyarakat Progresif Humanis Brainstormin
Publisher : Politeknik Harapan Bersama

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30591/japhb.v6i3.4608

Abstract

Akuaponik memiliki prinsip kerja dengan mengalirkan air dari kolam ikan ketempat media tanam. Kemudian air akan kembali mengalir dalam kolam, begitu seterusnya. Air yang mengalir dari dalam kolam ikan yang kotor dan kaya unsur hara akan diserap oleh akar tanaman. Sistem tanam akuaponik ada beberapa jenis, diantaranya adalah Nutrient Film Techniquw (NFT) yang merupakan sistem budidaya dengan meletakkan akar tanaman pada lapisan air yang dangkal, Media Filled Beds (MFB) menggunakan kontainer diisi dengan media batu dari tanah liat, dan Deep Water Culture (DWC) sistem akuaponik dengan tanaman yang mengambang diatas air. Jenis system tanam akuaponik tersebut memiliki perbedaan mekanisme dengan kontruksi yang berbeda dan memberikan hasil panen yang berbeda pula. Masyarakat Desa Gunungpring memanfaatkan sumber daya air yang baik hanya untuk memelihara berbagai jenis ikan dalam kolam. Padahal kolam tersebut berpotensi digunakan sebagai budidaya tanaman sayuran yang bermanfaat untuk keluarga dan bisa menambah pemasukan ekonomi. Oleh kerana itu dilakukan inovasi dalam penerapan budidaya tanaman sayuran dengan akuaponik model kincir yang bisa menghemat air, menghemat lahan, serta memberikan sirkulasi pada air dalam kolam dan tanaman. Selain itu, akuaponik model kincir dapat menambah nilai estetika dan kreativitas dibidang pertanian.
Ethnobotany of Taro Plant Diversity (Colocasia esculenta L. Schott) in Magelang City and Regency as a Biological Reference Book Wakhidatunnisa, Yunika Nurma; Kusumaningrum, Serafica Btari Christiyani; Darmawan, Ericka; Wahyudiningsih, Tri Suwarni
Biosfer: Jurnal Pendidikan Biologi Vol. 18 No. 1 (2025): Biosfer: Jurnal Pendidikan Biologi
Publisher : Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/biosferjpb.47491

Abstract

The diversity of taro plants in the Magelang area has been used for a long time by traditional communities from generation to generation. But knowledge related to the use of taro plants for purposes other than food is not widely known to the public. This study aims to determine the untilization of taro plants (Colocasia esculenta L. Schott) and analyze the feasibility of ethnobotanical reference books of taro plant diversity (Colocasia esculenta L. Schott) in Magelang City and Regency. This study is a type of mixed method research, which is a combination of ethnographic research methods with Research and Development (R&D) research. The results of ethnographic research are then used as a source of reading through the development of reference books. Development research was carried out using a 4-D research model and then limited to 3-D (define, design, and develop) due to time constraints. Based on the research that has been carried out, it is known that there are 5 varieties of taro plants (Colocasia esculenta L. Schott) that are found and used by the local people of Magelang, namely Taro Pratama 1, Pratama 2, Pratama 3, Taro Water/Bogor, and black Taro which are used by the community for various life purposes. The reference book based on the ethnobotanical study of taro plant diversity developed is suitable for use as a biological reference book with a percentage of 91% in material experts with excellent category, 83% in media experts with good category, and 95.89% in the results of community response tests with excellent category.
Konservasi Coelogyne pandurata Lindh. di Kalimantan Tengah: Karakter Morfologi, Propagasi In Vitro, dan Pelestarian Berbasis Komunitas Lokal Tri Suwarni Wahyudiningsih; Y Jagau; N Ravenska
Jurnal Pengelolaan Lingkungan Berkelanjutan (Journal of Environmental Sustainability Management) JPLB, Vol 2, No 2 (2018)
Publisher : Badan Kerjasama Pusat Studi Lingkungan (BKPSL) se-Indonesia bekerjasama dengan Pusat Penelitian Lingkungan Hidup IPB (PPLH-IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36813/jplb.2.2.125-139

Abstract

C. pandurata merupakan anggrek endemik, termasuk Appendiks I menurut CITES, dan mempunyai nilai ekonomi tinggi. Kebakaran hutan, konversi lahan, eksploitasi tidak terkendali, periode berbunga sangat pendek, dan sulit disilangkan menjadi faktor penyebab yang membuat populasi C pandurata terancam punah sehingga perlu dilakukan konservasi. Penelitian ini bertujuan untuk mengkaji perbedaan karakter morfologi C. pandurata dari Muara Teweh dan Tewah, propagasi secara in vitro, serta pelestarian berbasis komunitas masyarakat lokal. Bunga asal Tewah memiliki ciri-ciri seperti warna lidah lebih hitam, bulu halus dan ornamen lebih jelas, panjang dan lebar pada bulbus dan daun asal Tewah lebih besar. Eksplan serbuk biji ditanam pada medium perlakuan: I. MS (kontrol), II. MS + 3 mg/l BAP (Benzyl Amino Purine), III. MS + 3 mg/l BAP + Pisang, IV. MS + 3 mg/l BAP + air kelapa, dan V. MS + 3 mg/l BAP + tomat. Pada 10 HST (Hari Setelah Tanam) terjadi perubahan warna biji kuning menjadi hijau. Pro-meristem mengalami diferensiasi menjadi kutub calon akar pada suspensor dan kutub calon tunas. Protocorm tumbuh pada 21 HST. Seedling tumbuh pada 8 MST (Minggu Setelah Tanam) pada perlakuan I, II, dan IV. Pada perlakuan III seedling dengan tunas 0,5 cm terlihat pada 9 MST. Pada perlakuan V seedling tumbuh pada 11 MST dengan perawakan sehat dan kuat. Komunitas masyarakat lokal dan hutan adat menjadi prioritas awal pembinaan dan pemberdayaan melalui kegiatan pengenalan anggrek, teknik budi daya, pemahaman konservasi serta penanaman ke habitat, sehingga konservasi in-situ dapat berjalan secara berkesinambungan.
Analisis Keragaman Genetik Plasma Nutfah Tanaman Kayu Putih (Melaleuca cajuputi subsp. cajuputi) Berdasarkan Karakter Morfologi dan Anatomi Daun serta Oil Glands Aswin Hendry Atmoko; Tri Suwarni Wahyudiningsih; Noor Khomsah Kartikawati
JURNAL TRITON Vol 14 No 2 (2023): JURNAL TRITON
Publisher : Politeknik Pembangunan Pertanian Manokwari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47687/jt.v14i2.699

Abstract

Tanaman kayu putih merupakan tanaman hutan asli Indonesia yang dimanfaatkan untuk produksi minyak atsiri. Keragaman genetik tanaman kayu putih yang tersebar di zona persebaran alami cukup beragam. Diperlukan adanya studi dan penelitian untuk mempelajari hubungan kekerabatan antar tanaman kayu putih dan keragaman genetik plasma nutfah tanaman kayu putih. Studi kekerabatan dan juga keragaman genetik plasma nutfah didasarkan pada kemiripan karakter fenetik (kuantitatif dan kualitatif) morfologi dan anatomi daun. Terdapat 36 klon unggul tanaman kayu putih yang dilakukan pengamatan morfologi dan anatomi daun untuk menentukan dendogram. Penelitian yang dilakukan merupakan penelitian deksriptif dan juga penelitian eksperimental yang disusun dalam Rancangan Acak Lengkap (RAL) non faktorial dengan faktor yaitu macam klon yang terdiri atas 36 taraf (macam klon) dan dilakukan analisis sidik ragam menggunakan ANOVA dan dilakukan uji lanjut menggunakan uji Duncan’s. Dendogram dibuat menggunakan aplikasi MVSP versi 3.22. Hasil penelitian menunjukkan bahwa keragaman genetik yang luas pada 36 klon unggul tanaman kayu putih. Pada dendogram terbentuk 4 klaster dengan 8 klon yang menunjukkan tingkat kemiripan yang tinggi berdasarkan nilai disimilaritas sebesar 0,0. klon 14 dan 36 merupakan klon dengan rerata jumlah kelenjar minyak tertinggi. Berdasarkan penelitian yang dilakukan dapat dipahami bahwa keragaman genetik pada 36 klon unggul tanaman kayu putih cukup tinggi, hal tersebut mendukung upaya pemuliaan tanaman karena database yang diperoleh memberikan informasi klon yang dapat digunakan sebagai indukan untuk meningkatkan kemampuan produksi minyak kayu putih karena memiliki potensi produksi minyak yang tinggi.
Morphological Characteristics and Similarity Analysis of Cassava (Manihot esculenta Crantz) in Wonosobo, Temanggung, and Magelang Regencies Sugiyarto, Sugiyarto; Wahyudiningsih, Tri Suwarni; Darmawan, Ericka; Hidayah, Listya
Jurnal Biodjati Vol 8 No 2 (2023): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v8i2.25660

Abstract

The diversity of cassava in Wonosobo, Temanggung and Magelang districts must be investigated. The objective of the study was to analyze the diversity of cassava based on morphological characteristics. The cassava of each accession was planted in Bandongan, Magelang Regency. Morphological characters observed after six months of planting included apical leaf color, apical leaf hair, leaf lobe shape, petiole color, leaf color, number of leaf lobes, leaf length, leaf width, petiole length, vein color, petiole orientation, color epidermis of stem, color of outer appearance of stem, distance between nodes, stem growth, color of mature branch tip, branching type, tuber, tuber shape, outer tuber skin color, tuber flesh color, and tuber inner skin color. Analysis of the morphological characteristics of cassava is presented in the form of a dendrogram using the MVSP (Multi Variate Statistical Package) version 3.22. The results of the study obtained 39 accessions of cassava which showed different morphological characteristics. The lowest morphological similarity (0.735) was found in Group I only consisted of 2 acquisitions that did not have tubers, namely Red Vegetables and Green Vegetables and Group II ( Groups III and IV) have a similarity of 0.92. The highest similarity was in groups IIIa and IIIb and Groups IVa and IVb have a similarity coefficient value of 0.95. The morphological diversity of 39 accessions is a source of cassava germplasm: 37 accessions are harvested for tubers and 2 accessions areharvested explicitly for leaves as vegetables. This research was useful for the development of cassava-based food industry bioproducts.
Pemanfaatan Anggrek Spesies Kalimantan Tengah Berbasis Kearifan Lokal yang Berpotensi sebagai Bahan Obat Herbal Wahyudiningsih, Tri Suwarni; Nion, Yanetri Asi; ., Pahawang
Jurnal Biodjati Vol 2 No 2 (2017): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v2i2.1570

Abstract

Pemanfaatan anggrek spesies dari Kalimantan Tengah yang berpotensi sebagai bahan obat herbal berbasis kearifan lokal perlu dikaji. Penelitian dilakukan mulai bulan Oktober hingga Desember 2016. Tempat penelitian: koleksi anggrek spesies Kalimantan Tengah di Jl.Temanggung Tilung XIII Palangka Raya. Pemanfaatan anggrek spesies berdasar kearifan lokal berasal dari hasil wawancara pemilik kebun yang didukung  dengan  data  hasil  studi  literatur.  Spesies  anggrek  dari Kalimantan Tengah yang bermanfaat sebagai bahan obat herbal adalah batang dan daun anggrek tewu tadung/anggrek tebu (Grammatophylum speciosum) sebagai bahan obat kista dan uwei menyame (Bromheadia finlaysoniana (Lind.) Miq.) sebagai salah satu komponen obat sakit pinggang. Beberapa anggrek spesies yang berpotensi sebagai bahan obat herbal  dan  fitoterapi  berdasar  studi  literatur  antara  lain:  rhizome anggrek bambu (Arundina graminifolia (D. Don) Hochr) mengandung senyawa Arundinan mempunyai aktivitas anti bakteri. Daun Phalaenopsis manii  mengandung  phalaenopsine.  Seluruh  bagian  tumbuhan  Eria bambusifolia  Lindl.  Kimar  digunakan  untuk  mengatasi  keasaman lambung yang berlebihan dan gangguan sakit perut. Anggrek Coelogyne cristata   mengandung   Coeloginanthrin,   Coeloginanthridin,   dan Combretastatin  C-1.  Daun  Dendrobium  crumenatum  Sw.  (anggrek merpati) dapat digunakan untuk tapal pada bisul dan jerawat.
Pengaruh Variasi Klon Unggul dan Bahan Pemacu Akar terhadap Pertumbuhan Stek Pucuk Melaleuca cajuputi subsp. cajuputi: The Effect of Superior Clone Variations and Root Growth Promoters on the Growth of Melaleuca cajuputi subsp. cajuputi Shoot Cuttings Soviana, Farah; Wahyudiningsih, Tri Suwarni; Kartikawati, Noor Khomsah
JURNAL HUTAN TROPIKA Vol 20 No 2 (2025): Volume 20 Nomor 2 Tahun 2025
Publisher : Jurusan Kehutanan, Fakultas Pertanian Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36873/jht.v20i2.22873

Abstract

Melaleuca cajuputi subsp. cajuputi has strong potential for development in the agribusiness sector, requiring appropriate cultivation strategies such as the selection of superior clones. This study was conducted from February to June 2025 at the Forest Plant Seed Certification and Nursery Center, Kalongan Nursery, Semarang. The research focused on the nursery stage, a critical phase in producing high-quality planting materials. The objective was to identify the most optimal clone and rooting stimulant for propagation via cuttings, using a Completely Randomized Block Design (CRBD). The first factor was ten superior clones, and the second was two rooting stimulants (Rootone F and Growtone). The overall survival rate of cuttings was 77.50%, with a rooting rate of 70.42%. Clone variation had a highly significant effect on cutting height, number of leaves, and number of roots, and a significant effect on axillary shoot number and root length. Clone 1, from Maluku, showed the best vegetative growth. The type of rooting stimulant and its interaction with clone variation had no significant effect on any measured parameter.