Article Per Year (5 Year)
p-Index From 2021 - 2026
0.61
P-Index
This Author published in this journals
All Journal Indonesian Journal of Biotechnology The Indonesian Biomedical Journal IGKOJEI: Jurnal Pengabdian Masyarakat
Hevi Wihadmadyatami
Departemen Anatomi, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Materials Science & Nanotechnology Public Health Social Sciences Veterinary

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : The Indonesian Biomedical Journal

 1 
2-(3-(chloromethyl)benzoyloxy)benzoic Acid Increases CD4+ Regulatory T-Cell Population and FoxP3 Expression in Lipopolysaccharide-induced Mice Yudy Tjahjono; Caroline Caroline; Jusak Nugraha; Kuncoro Foe; Srikanth Karnati; Süleyman Ergün; Nico Jafet; Oryza Chrisantia; I Made Andika Bara Kusuma; Hendy Wijaya; Wuryanto Hadinugroho; Dwi Aris Agung Nugrahaningsih; Dwi Liliek Kusindarta; Yufita Ratnasari Wilianto; Senny Yesery Esar; Hevi Wihadmadyatami
The Indonesian Biomedical Journal Vol 15, No 4 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i4.2429

Abstract

BACKGROUND: Lipopolysaccharide (LPS) has been reported to increase CD4+ regulatory T-cell (CD4+ Treg) populations. Acetylsalicylic acid (ASA) has been reported to have immunomodulatory activity, but it may induce chronic gastric ulceration. Another salicylic acid-bearing compound, 2-(3-(chloromethyl)benzoyloxy)benzoic acid (3-CH2Cl), has been reported to have less gastric mucosal damage. However, the effect of 3-CH2Cl on CD4+ Tregs in LPS-induced mice is still unknown. Therefore, the present study was conducted to investigate the immunomodulatory effect of 3-CH2Cl on CD4+ T-cell and CD4+ Treg populations as well as FoxP3 expression in LPS-induced mice.METHODS: Synthesis of 3-CH2Cl was performed by mixing salicylic acid and chloromethylbenzoylchloride with the catalyzation of pyridine, acetone and heat. The 3-CH2Cl tablets were prepared using direct compression method. After intraperitoneal injection of 1 mg/kg BW LPS to mice, 60 mg/kg BW ASA or 60 mg/kg BW 3-CH2Cl was given orally for 3 days. The splenocyte was obtained through splenectomy and collagenase digestion. The population of CD4+ T-cells and CD4+ Tregs, as well as the splenic FoxP3 expression were determined using flow cytometry technique.RESULTS: CD4+ T-cell populations in mice treated with LPS and 3-CH2Cl or ASA were lower than those treated with LPS merely. Meanwhile, CD4+ Treg populations and FoxP3 expression levels in mice treated with LPS and 3-CH2Cl or ASA were higher than those treated with LPS merely.CONCLUSION: Since 3-CH2Cl could decrease CD4+ T-cell population and increase CD4+ Treg population mediated by the increase of FoxP3 expression in LPS-induced inflammation, it may act as a potential therapeutic drug to reduce inflammatory conditions.KEYWORDS: 2-(3-(chloromethyl)benzoyloxy)benzoic acid, acetylsalicylic acid, CD4, T-regulatory cells, FoxP3, LPS
Ocimum sanctum Leaves Prevent Neuronal Cell Apoptosis Through Reduction of Caspase-3 and -9 Expressions and Inhibition of β-amyloid Oligomerization Dinda Aliffia; Dinda Ayu Ramadhani; Widya Wasityastuti; Dewi Ratih Tirto Sari; Ulayatul Kustiati; Hevi Wihadmadyatami; Dwi Liliek Kusindarta
The Indonesian Biomedical Journal Vol 15, No 4 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i4.2415

Abstract

BACKGROUND: Neurodegenerative diseases are characterized by the loss of neuronal function in the nervous system. In recent years, more than 45 million people worldwide have suffered from progressive loss of memory and cognitive functions caused by Alzheimer’s disease. Ocimum sanctum is one of the medicinal plants known to have neuroprotective abilities. This study was conducted to elucidate the anti-apoptotic effects of ethanolic extract of O. sanctum (EEOS) on PC12 and SH-SY5Y cells as well as interaction between main compounds of EEOS and β-amyloid (Aβ) peptide through in silico molecular docking.METHODS: The viability of TMT-induced PC12 and SH-SY5Y cells was assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and acridine orange/propidium iodide staining. Cell proliferation rate was measured with cell counting kit-8 (CCK-8) assay. Nuclear fragmentation was observed with Hoechst 33342 staining. Caspase -3 and -9 expressions were measured using enzyme-linked immunosorbent assay. Interactions between main compounds of EEOS and Aβ were visualized with in silico molecular docking.RESULTS: EEOS had the potential effect of maintaining cell viability, preventing the cell’s morphological changes, and inhibiting apoptosis via the caspase pathway in PC12 and SH-SY5Y cells. Meanwhile, flavonoid K, phenol, eugenol could interact with the active site of Aβ through hydrogen-bonding and hydrophobic interactions.CONCLUSION: EEOS could prevent neuronal cell apoptosis via downregulation of caspase-3 and -9. Main compounds of EEOS could interact with the active site of Aβ, and thereby might inhibit Aβ oligomerization. Thus, EEOS and its main compounds could be potential as neuroprotective agents for preventing neurodegenerative diseases.KEYWORDS: Ocimum sanctum, anti-apoptotic, β-amyloid, caspase, neurodegeneration
Co-Authors Ariana Aris Haryanto Arvendi Rachma Jadi Budiariati, Vista Caroline Caroline Dewi Ratih Tirto Sari Dinda Aliffia Dinda Ayu Ramadhani Dwi Aris Agung Nugrahaningsih Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Foe, Kuncoro Hendy Wijaya Hery Wijayanto I Made Andika Bara Kusuma Jusak Nugraha Nastiti Wijayanti Nico Jafet Oryza Chrisantia Senny Yesery Esar Srikanth Karnati Süleyman Ergün Teguh Budipitojo Tri Wahyu Pangestiningsih Ulayatul Kustiati Widya Wasityastuti Wuryanto Hadinugroho Yudy Tjahjono Yufita Ratnasari Wilianto