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All Journal HAYATI Journal of Biosciences Hemera Zoa Jurnal Sain Veteriner Biotika: Jurnal Ilmiah Biologi Journal of Tropical Biodiversity and Biotechnology IGKOJEI: Jurnal Pengabdian Masyarakat
Budiariati, Vista
Faculty Of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Social Sciences Veterinary

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Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium Budiariati, Vista; Budiono, Dwi; Fahrudin, Mokhamad; Juliandi, Berry; Rinendyaputri, Ratih; Boediono, Arief
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.143 KB) | DOI: 10.4308/hjb.27.2.89

Abstract

Parthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst.
Production and Characterization of Mouse Diploid Parthenogenetic Blastocyst Developed in Phosphate-Free Medium Vista Budiariati; Dwi Budiono; Mokhamad Fahrudin; Berry Juliandi; Ratih Rinendyaputri; Arief Boediono
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (396.143 KB) | DOI: 10.4308/hjb.27.2.89

Abstract

Parthenogenesis is an artificial oocytes activation process without paternal contribution. Blastocyst, derived from parthenogenesis, is one of potential source for pluripotent stem cell propagation. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate. One of the component that predicted inhibit parthenogenetic embryo development is phosphate. Therefore, we try to modify culture medium in order to overcome that problem. The aim of this research was to produce and analyze the characteristics of parthenogenetic blastocyst developed in phosphate-free medium. Mouse oocytes obtained from adult female DDY by superovulation. The activator was strontium chloride 10 mM and diploidization with cytochalasin B 5 μg/ml. Medium for activation and culture medium were modified rat 1 cell embryo medium (MR1ECM) which is phosphate free. The results showed that parthenotes that were cultured in phosphate free medium reached higher blastocyst rate compared to the other groups. The increase of phosphate in culture medium lead to impaired parthenogenetic embryos development. Further experiment was made to analyze the differences between fertilized and parthenogenetic embryo in this medium. The experiment showed that diploid parthenogenetic could achieve high blastocyst rate (30.9±1.3%). The quality of diploid parthenogenetic blastocyst, based on cells number, viability, and ICM ratio, was lower than fertilized blastocyst.
Interspecies Reconstructed Embryonic Cell Interaction between Campbell Hamster (Phodopus campbelli) and Mice (Mus musculus) Noer Muhammad Dliyaul Haq; Diah Pristihadi; Vista Budiariati; Dwi Budiono; Al Mukhlas Fikri; Mokhamad Fahrudin; Cece Sumantri; Arief Boediono
HAYATI Journal of Biosciences Vol. 28 No. 3 (2021): July 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.3.185

Abstract

Interspecies embryo transfer is a seldom-used method to increase the successful conservation of endangered species. The study aimed to determine the potential development of interspecies reconstructed embryos. The present study used two animal models, Campbell hamsters (Phodopus campbelli) and mice (Mus musculus). The isolated inner cell mass (ICM) of hamster embryos were injected into the mice embryos. The embryos were transferred to the pseudopregnant mice using non-surgery embryos transfer methods. The fetuses were collected at day 13.5 of gestation for morphometric measurement and cytochrome b (Cyt b) analysis which used to determine the species of obtained fetuses. The results showed that the viability, pregnancy rate, and embryonic implantation ability of the interpecies reconstructed embryos did not differ significantly (p>0.05) compared to non-reconstructed embryos. Morphometric measurement showed that the crown-rump (CR) and the weight of fetuses in the reconstructed group were significantly higher than non-reconstructed group (p<0.05). According to Cytb analysis, the species of obtained fetuses were mice, while the population of hamster cells were found only in the blighted ovum (resorption). Therefore, it can be concluded that interspecies reconstructed embryos are able to implant. However, the population of mice cells are only found to develop.
PCS-1 Development of Mouse Parthenogenetic Embryos in Phosphate Free Medium Vista Budiariati; Dwi Budiono; Mokhamad Fahrudin; Berry Juliandi; Ratih Rinendyaputri; Arief Boediono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (516.416 KB)

Abstract

Parthenogenesis is an artificial oocytes activation process without paternal contribution so that embryos will develop without fertilization [3]. The process of parthenogenesis as a reproductive strategy occurs in species of insect, pisces, or amphibian, which not require any implantation. Naturally, parthenogenesis is not common in mammals, but by understanding cellular mechanism during fertilization, it is possible to artificially activate mammalian oocytes.Blastocyst, derived from parthenogenesis, can be used for developmental study, embryo reconstruction, and one of potential source for pluripotent stem cells. Unfortunately, previous studies reported that parthenogenetic embryo did not achieve exhilarating blastocyst rate.One of the component that has been predicted to inhibit parthenogenetic embryo development is phosphate. Haraguchi et al. (1996)    reported that phosphate caused a negative effect on in vitro culture of AKR/N mice fertilized embryos, removal of phosphate elements was significantly improved the blastocyst rate up to 42.6% [1]. The effects of phosphate also became an interesting finding in the study that reported mouse fertilized embryos could well developed in modified medium rat 1 cell embryo medium (MR1ECM) which not contained any phosphate [2].The effect of phosphate on in vitro culture of mouse parthenogenetic embryo has not been clear. The aim of this research was to analyze inhibitory effect caused by phosphate in the medium and compare the development pattern between parthenogenetic and fertilized embryos in order to reach optimal production of parthenogenetic blastocyst for further purposes.  
PENDAMPINGAN PEMBELAJARAN BIOLOGI ANATOMI HEWAN SECARA DARING UNTUK SEKOLAH-SEKOLAH DASAR GUGUS VI DI DESA TUKSONO, SENTOLO, KULON PROGO, YOGYAKARTA : Online Animal Anatomy Biology Learning Assistance for Cluster VI Elementary Schools in Tuksono Village, Sentolo, Kulon Progo, Yogyakarta Arvendi Rachma Jadi; Hery Wijayanto; Dwi Liliek Kusindarta; Vista Budiariati; Tri Wahyu Pangestiningsih; Teguh Budipitojo; Ariana; Hevi Wihadmadyatami
IGKOJEI: Jurnal Pengabdian Masyarakat Vol. 3 No. 2 (2022): IGKOJEI: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/igkojei.v3i2.301

Abstract

ABSTRACT  The problem faced during the new normal period of the Covid-19 pandemic for students is to restore interest in learning that has been done at home. Additional efforts are needed to reduce these concerns, such as mentoring for learning outside of school. Community service in the form of learning assistance is carried out with the aim of providing new experiences, information, and knowledge outside of learning at school. The schools participating in the service activities are schools from Cluster VI Tuksono Village, Sentolo, Kulon Progo, Yogyakarta. This activity involves student assistants from the Anatomy Department, Faculty of Veterinary Medicine, Universitas Gadjah Mada with assistance for a certain period. Implementation of online activities providing materials and discussions that have been adapted to the competencies needed in schools. The material given is an introduction to vertebrates macroscopically and microscopically. Students will be divided into several classes and each has an assistant and a co-lecturer. The activity takes place from September to November 2021. The results of the questionnaire show that this learning assistance activity is very interesting (60%) and very useful (69%) for students and teachers in their schools.  Keywords: Animal, Biology Anatomy; Elementary school; Learning; Online   ABSTRAK  Permasalahan yang dihadapi pada masa normal baru pandemi Covid-19 bagi peserta didik adalah mengembalikan minat belajar yang selama ini dilakukan di rumah. Usaha untuk mengurangi keprihatinan tersebut diperlukan seperti pendampingan belajar luar sekolah. Pengabdian kepada masyarakat berupa pendampingan pembelajaran dilaksanakan dengan tujuan memberikan pengalaman, informasi, dan pengetahuan baru di luar pembelajaran di sekolah. Sekolah yang berpartisipasi dalam kegiatan pengabdian adalah sekolah-sekolah dari Gugus VI Desa Tuksono, Sentolo, Kulon Progo, Yogyakarta. Kegiatan ini melibatkan asisten mahasiswa Departemen Anatomi Fakultas Kedokteran Hewan Universitas Gadjah Mada dengan pendampingan selama periode tertentu. Pelaksanaan kegiatan daring pemberian materi dan diskusi yang sudah disesuaikan dengan kompetensi yang dibutuhkan di sekolah. Materi yang diberikan berupa pengenalan vertebrata secara makroskopik dan mikroskopik. Peserta didik akan dibagi menjadi beberapa kelas dan masing-masing memiliki satu asisten dan dosen pendamping. Kegiatan berlangsung dari bulan September sampai November 2021. Hasil kuisioner menunjukkan kegiatan pendampingan pembelajaran ini sangat menarik (60%) dan sangat bermanfaat (69%) bagi para peserta didik dan guru di sekolahnya. Kata kunci: Anatomi Biologi; Daring; Hewan; Pembelajaran; Sekolah Dasar
KOMPARASI OOSIT DAN HISTOLOGI OVARIUM MENCIT DENGAN SUPEROVULASI HORMONAL DAN KOMBINASI INDUKSI OVULASI Budiariati, Vista; Damayanti, Safira; Lukmana, Shafara Widyanti
BIOTIKA Jurnal Ilmiah Biologi Vol 21, No 1 (2023): BIOTIKA JUNI 2023
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v21i1.44821

Abstract

Koleksi oosit merupakan langkah penting dalam produksi dan rekayasa embrio in vitro. Proses ini dimulai dengan superovulasi untuk memaksimalkan jumlah oosit. Superovulasi yang paling umum dilakukan yaitu dengan induksi hormonal menggunakan kombinasi pregnant mare serum gonadotropin (PMSG) dan human chorionic gonadothropin (hCG). Penelitian ini bertujuan untuk membandingkan hasil superovulasi mencit dengan induksi hormonal dan kombinasi stimulus kawin. Penelitian ini merupakan penelitian eksperimental dengan hewan coba mencit yang dibagi menjadi dua kelompok. Kelompok A yaitu kelompok mencit yang disuperovulasi dengan induksi hormonal injeksi PMSG dilanjutkan dengan hCG sedangkan kelompok B diinjeksi dengan PMSG dilanjutkan dengan stimulus kawin menggunakan mencit jantan yang telah divasektomi. Parameter yang dianalisis dalam penelitian ini yaitu jumlah dan morfologi oosit serta histologi ovarium dari kedua kelompok. Hasil penelitian ini menunjukkan tidak ada perbedaan yang signifikan antara total oosit dari perlakuan A dan perlakuan B. Persentase oosit matang dari kelompok B lebih tinggi dibandingkan kelompok A. Histologi ovarium kelompok A menunjukkan perkembangan folikel yang bervariasi sedangkan kelompok B didominasi oleh korpus luteum. Berdasarkan hasil perbandingan oosit dan histologi ovarium dapat disimpulkan bahwa metode superovulasi dengan kombinasi stimulus kawin dapat digunakan sebagai alternatif metode untuk mengantisipasi keterbatasan jumlah preparat hormon pada proses produksi dan rekayasa embrio in vitro.
Karakteristik Pemeliharaan Kuda Andong di Pantai Parangtritis dan Malioboro Pranaja, Mohammad Rakha Surya; Amperawati, Yashinta; Faiza, Aliyya Nur; Aliyyah, Nabila Shafa Nur; Wenni, Asyam Hafidz; Savitri, Azalea Perwita; Najwa, Nabila Febriyana; Agatha, Nur Lailya; Annisa, Syifa; Budiariati, Vista
Jurnal Sain Veteriner Vol 42, No 3 (2024): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.100737

Abstract

Kuda andong merupakan salah satu ikon khas Yogyakarta dan memiliki peran besar dalam menarik perhatian wisatawan. Beberapa tempat wisata di Yogyakarta yang menyediakan kuda andong adalah Pantai Parangtritis dan Malioboro. Performa kuda, khususnya pada kuda pekerja dipengaruhi oleh beberapa manajemen pemeliharaan yang saling berkaitan. Manajemen pemeliharaan yang buruk dapat mengakibatkan kuda mudah terinfeksi suatu penyakit, malnutrisi, dan produktivitas rendah. Tujuan dari penelitian ini untuk mengetahui karakteristik pemeliharaan kuda andong di Pantai Parangtritis dan Malioboro guna mengidentifikasi masalah pemeliharaan kuda andong yang ada pada wilayah tersebut beserta solusinya. Pengumpulan data dilakukan dengan pengisian kuesioner oleh 51 kusir andong di Pantai Parangtritis dan Malioboro. Kuesioner terdiri dari 27 pertanyaan terkait manajemen pemeliharaan kuda andong. Selain itu, juga dilakukan pengambilan sampel feses kuda andong untuk pemeriksaan dengan metode sentrifus dan McMaster. 
Morphological Structure of the Tongue of Gekko gecko in Yogyakarta, Indonesia Aqiilah, Khairunnisa; Budipitojo, Teguh; Wijayanto, Hery; Budiariati, Vista; Pangestiningsih, Tri Wahyu; Ariana, Ariana; Saragih, Golda Rani; Kustiati, Ulayatul; Wihadmadyatami, Hevi
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 4 (2024): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.90995

Abstract

Gekko gecko is a member of the order Squamata from the family Gekkonidae and mainly feeds on small insects. This study aims to determine the morphology of the tongue of Gekko gecko through Scanning Electron Microscopy (SEM), hematoxylin–eosin (HE) staining, and immunohistochemistry (IHC). Six adult Gekko gecko were obtained from the Special Region of Yogyakarta, and Gekko gecko tongue samples were stored in SEM fixative solution and then observed with SEM. For histochemical and IHC staining, tongue samples were processed into paraffin blocks and cut into 8 µm-thick sections. The SEM revealed three types of papillae: dome-shaped papillae at the apex, fan-shaped papillae at the corpus, and scale–like papillae at the radix. Histological observations showed that the tongue of the Gekko gecko was composed of tunica mucosa and tunica muscularis, and goblet cells were present in the lamina of the epithelial mucosa. Meanwhile, no taste buds were found. Immunoreactivity against PGP 9.5 was observed on the tunica muscularis of the apex, corpus, and radix. Taken together, this study provides new insight into the tongue morphology of Gekko gecko and is dominated by mechanical papillae on the tongue surface.
Co-Authors Agatha, Nur Lailya Al Mukhlas Fikri Aliyyah, Nabila Shafa Nur Amperawati, Yashinta Annisa, Syifa Aqiilah, Khairunnisa Ariana Ariana Ariana Arief Boediono Arvendi Rachma Jadi Berry Juliandi Cece Sumantri Damayanti, Safira Diah Nugrahani Pristihadi Dwi Budiono Dwi Liliek Kusindarta Faiza, Aliyya Nur Hery Wijayanto Hevi Wihadmadyatami Hevi Wihadmadyatami Kustiati, Ulayatul Lukmana, Shafara Widyanti Mokhamad Fahrudin Najwa, Nabila Febriyana Noer Muhammad Dliyaul Haq Pranaja, Mohammad Rakha Surya Ratih Rinendyaputri Ratih Rinendyaputri Ratih Rinendyaputri Saragih, Golda Rani Savitri, Azalea Perwita Teguh Budipitojo Teguh Budipitojo Tri Wahyu Pangestiningsih Wenni, Asyam Hafidz