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ANTIBACTERIAL EFFECT OF NANNOCHLOROPSIS OCULATA AS ROOT CANAL STERILIZATION MATERIAL ON STREPTOCOCCUS MUTANS BIOFILM Diana Soesilo; Sinta Puspita; Phebe Fedora Christabel
ODONTO : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.8.2.119-125

ABSTRACTBackground: Streptococcus mutans in the most frequent microbiota that causes pulp necrosis because of caries. The microorganism that is colonized and embedded in the biofilm matrix is resistant to antimicrobials compared to planktonic cells. Root canal sterilization materials must have good biocompatibility with tissues. Nannochloropsis oculata is an algae that contains various compounds such as terpenoids, alkaloids, and flavonoids that have potential as antibacterial and antioxidant and can be used as alternative to root canal sterilization. Method: This research was true experimental laboratory research with post-test only control group design. The antibacterial potential of Nannochloropsis oculata was tested using the biofilm method, divided into 5 groups. The control group was: K- (aquadest), K+ (calcium hydroxide), and the treatment group was given Nannochloropsis oculata: P1 (0.625%), P2 (1.25%), and P3 (2.5 %). Congo Red method test was to determine the formation of biofilm that shows black strains on agar. While biofilm test with Microtiter Plate Assay to measure the value of biofilm that were inhibited in Optical Density (OD) value in the ELISA Reader. The lower the value, the more biofilm inhibited, with OD value, inhibition percentage could counted Result: The result of all treatment groups were increasing in percentage inhibition value shows inhibition in biofilm growth (p <0.05). Conclusion: Nannochloropsis oculata had an antibacterial effect on the biofilm of Streptococcus mutans

Peranan sorbitol dalam mempertahankan kestabilan pH saliva pada proses pencegahan karies (The role of sorbitol in maintaining saliva’s pH to prevent caries process) Diana Soesilo; Rinna Erlyawati Santoso; Indeswati Diyatri
Dental Journal (Majalah Kedokteran Gigi) Vol. 38 No. 1 (2005): March 2005
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

People in Indonesia often consume food containing sucrose. If the sucrose consumed is in a large amount, it will decrease saliva’s pH and soon teeth destruction will happen. To avoid it, it is necessary to change sucrose consumption habit into another kind of sugar, namely sorbitol. Sorbitol is preferred to use, because it is cheaper and easier to get. Sorbitol is made from cassava, which is plentifully grown in Indonesia. Sorbitol is not good media for bacteria to grow. Because sorbitol has a diol, so it’s difficult to catalyst by glucosyltransferase enzyme, which is produced by bacteria Streptococcus mutans. The conclusion is that sorbitol is difficult to be fermented by Streptococcus mutans so it will not decrease saliva’s pH.

Perbandingan daya antibakteri serat selulosa sabut kelapa (Cocos nucifera L.) pada konsentrasi berbeda terhadap Streptococcus mutansComparison of the antibacterial power of coconut cellulose fiber (Cocos nucifera L.) at different concentrations against Streptococcus mutans Sinta Puspita; Diana Soesilo; Linda Rochyani; Twi Agnita Cevanti
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 34, No 1 (2022): April 2022
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v34i1.35076

Pendahuluan: Kelemahan dari komposit konvensional adalah terjadinya shrinkage dan stress polimerisasi. Penggunaan serat sebagai bahan pengisi pada resin komposit dapat menurunkan kontraksi polimerisasi. Berdasarkan penelitian sebelumnya serat sabut kelapa memiliki daya antibakteri yang cukup baik karena mengandung golongan senyawa metabolit sekunder yaitu tanin, flavonoid, dan polifenol. Selain itu juga memiliki beberapa senyawa, antara lain asam elagat, asam galat, epikatekin, dan katekin yang juga diperkirakan memiliki aktivitas sebagai anti bakteri. Serat sabut kelapa tidak dapat digunakan secara langsung dalam bentuk aslinya sehingga dibutuhkan modifikasi untuk membersihkan serat. Tujuan penelitian menganalisis perbandingan daya antibakteri serat selulosa sabut kelapa (Cocos nucifera L.) pada konsentrasi berbeda terhadap S. mutans. Metode: Jenis penelitian true experimental dengan desain penelitian posttest only control design. Ekstraksi serat selulosa dari sabut kelapa melalui proses bleaching kemudian sintesis selulosa menggunakan NaOH dan urea selanjutnya di nukleasi dengan etanol sebagai anti solvent organik dan dikeringkan dengan proses sublimasi. Pengujian aktivitas antibakteri menggunakan metode sumur difusi dengan dua konsentrasi uji yaitu kelompok 1 menggunakan anti solvent etanol 70% dan, pada kelompok 2 menggunakan etanol 96%. Kontrol negatif menggunakan aquadest steril. Selanjutnya diamati dan diukur diameter zona hambat dengan jangka sorong. Data yang diperoleh diuji statistik menggunakan independent t-test. Hasil: Daya antibakteri kelompok sampel yang diberi perlakuan etanol 96% lebih tinggi dibandingkan dengan kelompok sampel etanol 70%. Hasil uji independent t-test menunjukkan bahwa nilai p yang signifikan p=0,000<0,05, Simpulan: Serat sabut kelapa yang diberi etanol dengan konsentrasi 96% terbukti memiliki daya antibakteri yang lebih tinggi daripada yang diberi etanol dengan konsentrasi 70%.Kata kunci: daya antibakteri; serat sabut kelapa; Streptococcus mutans ABSTRACTIntroduction: The weakness of conventional composites is the occurrence of shrinkage and polymerisation stress. The use of fiber as a filler in composite resins can reduce polymerisation contraction. Based on previous research, coconut fiber has good antibacterial properties because it contains a class of secondary metabolites, namely tannins, flavonoids, and polyphenols. In addition, it also has several compounds, including ellagic acid, gallic acid, epicatechins and catechins which are also thought to have antibacterial activity. However, coconut fiber cannot be used directly in its original form so modifications are needed to clean the fiber. This study aimed to compare the antibacterial power of coconut cellulose (Cocos nucifera L.) fibers at different concentrations against S. mutans. Methods: This type of research is true experimental with posttest only control design. Extraction of cellulose fiber from coconut fiber through a bleaching process and then synthesis of cellulose using NaOH and urea then nucleated with ethanol as an organic anti-solvent and dried by sublimation process. The antibacterial activity was tested using the diffusion well method with two test concentrations, namely group 1 using 70% ethanol anti-solvent and, in group 2 using 96% ethanol. Negative control using sterile distilled water. Then observed and measured the diameter of the inhibition zone with a caliper. The data obtained were statistically tested using independent t-test. Results: The antibacterial power of the sample group that was treated with ethanol was 96% higher than that of the 70% ethanol sample group. The results of the independent t-test obtained the p-value of p=0.000; p<0.05. Conclusion: Coconut fiber which was given ethanol with a concentration of 96%, proved to have higher antibacterial power than that given ethanol with a concentration of 70%.Keywords: antibacterial activity; coconut fiber; Streptococcus mutans

The Effectiveness Of Anchovy Concentration (Stolephorus insularis) as Antimicrobial to Streptococcus mutans (In Vitro) Almira Fa’Izah; Istien Wardani; Diana Soesilo
Denta Journal Kedokteran Gigi Vol 10 No 1 (2016): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar

Background: Dental and oral diseases which are often found in children is dental caries. Streptococcus mutans is the main cause of caries. Caries can be prevented by using a topical application of fluoride. The Anchovy (Stolephorus insularis) contains protein, vitamins (A, B1, C), and minerals (Fe, Ca, K, F). Calcium fluoride (CaF2) within the anchovy can inhibit the occurrence of dental caries. Purpose: The aim of this study was to determine the antimicrobial ability of anchovy extract (Stolephorus insularis) to Streptococcus mutans. Materials and Methods: This study was a laboratory experimental research with post test only control group design. Diffusion method were applied with 2 controls: negative control used DMSO 1%, positive control used NaF solution, and 3 concentrations of anchovy extract (Stolephorus insularis) 3%, 6%, and 12%, each group were composed of 6 samples. Antimicrobial was assessed by measuring the diameter of the clear zone around the discs contained the anchovy extract (Stolephorus insularis). Data were analyzed by Kruskal-Wallis test followed by Mann-Whitney test. Result: The results from this study showed clear zone around the discs of the anchovy extract (Stolephorus insularis). The more concentration of the extract showed the more antimirobial zone diameter. The average zone of antimicrobial at the concentration of 3% were 7,11 mm, 6% 9,5 mm, 12% 10,78 mm, for the negative control DMSO 1% 6 mm and the positive control NaF solution 8,16 mm. The largest diameter of the clear zone was at concentration of 12% (P < 0,05). Conclusion: The anchovy extract (Stolephorus insularis) had an antimicrobial effect to the growth of Streptococcus mutans.

Invitro Micro Encapsulation of Beta Tri Calcium Phosphate from Anadara granosa Shell Synthesis Diana Soesilo; Aprilia; Moh. Basroni Rizal
Denta Journal Kedokteran Gigi Vol 14 No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v14i2.3

Background: Calcium is a material that is mostly contained in the Anadara-granose shell. Beta-TCP can be obtained from the hydrothermal process from the Anadara-granose shell. Beta-TCP has a chemical composition that approximates the structure of bones and teeth. Objective:The microencapsulation technique aims to increase stability, reduce side effects and toxic effects of drugs, and prolong the release of ingredients. The encapsulation process is an attempt to inhibit the dissolution speed of Calcium to prevent tunnel defects. Methods: Anadara-granose shell powder was subjected to hydrothermal processing for 18 hours and sintering for 3 hours. The beta-TCP powder was dissolved with aquadest using a magnetic stirrer until it was homogeneous, Na-alginate was dissolved in aquadest until it was homogeneous with a magnetic stirrer then the two solutions were mixed and the CaCl2 solution was dropped. The sample was divided into 3 groups; Pure Beta-TCP(K-); 7 hours stirring (P1); 8 hours stirring (P2). After completion of the stirrer, the samples were centrifuged at 2500 rpm for 6 minutes, then freeze-dried for 12 hours. The level test was carried out using complexometry comparing the pure Beta-TCP group with the Beta-TCP stirrer encapsulation process for 7 hours and 8 hours. Results: The data showed that the average calcium level in K(-) group with pure Beta-TCP was 8.63%, the P1 Beta-TCP group with 7 hours stirrer 2.86%, and the P2 Beta-TCP group with 8 hours stirrer 2.12%. Conclusion: In the Anadara-granosa shell nanoencapsulation process, the calcium level gradually decreased with the longer duration of stirring time

Perawatan Internal Bleaching Untuk Estetik Gigi Pasca Perawatan Endodotik Diana Soesilo
Denta Journal Kedokteran Gigi Vol 10 No 2 (2016): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

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Background: In general, everyone likes white teeth as a symbol of beauty and health. Discoloration can be caused by extrinsic and intrinsic factors. One of the factors that cause teeth discoloration is due to the trauma that causes the teeth to be non vital. One alternative treatment that becomes an option is internal bleaching. Case: 37-year-old female patient with # 11 and # 21 non vital post-endodontic treatments and color changes to blackish color especially on cervical parts. Case Management: Gutta percha point taking 2-3 mm below orifice using peeso reamer. Application of Glass Ionomer type 3 cement over gutta percha point as base. Etching with 37% phosphoric acid for 20 second was then washed etching and dried with cotton pellet. The bleaching superoxol material (30-35% Hydrogen Peroxide) was appliedto the pulp chamber and then covered with cotton and Glass Ionomer type 2 cement to prevent leakage of bleaching material. Control is done once a week until the appropriate color of the tooth is obtained. Conclusion: Treatment that can be done to restore the color of the original tooth without making an excessive reduction to the dental crown is by internal bleaching.

ANTIBACTERIAL EFFECT OF NANNOCHLOROPSIS OCULATA AS ROOT CANAL STERILIZATION MATERIAL ON STREPTOCOCCUS MUTANS BIOFILM Diana Soesilo; Sinta Puspita; Phebe Fedora Christabel
Odonto : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

ABSTRACTBackground: Streptococcus mutans in the most frequent microbiota that causes pulp necrosis because of caries. The microorganism that is colonized and embedded in the biofilm matrix is resistant to antimicrobials compared to planktonic cells. Root canal sterilization materials must have good biocompatibility with tissues. Nannochloropsis oculata is an algae that contains various compounds such as terpenoids, alkaloids, and flavonoids that have potential as antibacterial and antioxidant and can be used as alternative to root canal sterilization. Method: This research was true experimental laboratory research with post-test only control group design. The antibacterial potential of Nannochloropsis oculata was tested using the biofilm method, divided into 5 groups. The control group was: K- (aquadest), K+ (calcium hydroxide), and the treatment group was given Nannochloropsis oculata: P1 (0.625%), P2 (1.25%), and P3 (2.5 %). Congo Red method test was to determine the formation of biofilm that shows black strains on agar. While biofilm test with Microtiter Plate Assay to measure the value of biofilm that were inhibited in Optical Density (OD) value in the ELISA Reader. The lower the value, the more biofilm inhibited, with OD value, inhibition percentage could counted Result: The result of all treatment groups were increasing in percentage inhibition value shows inhibition in biofilm growth (p <0.05). Conclusion: Nannochloropsis oculata had an antibacterial effect on the biofilm of Streptococcus mutans

SCHOOL HEALTH PROGRAM BERSAMA SD VITA SCHOOL UNTUK MEWUJUDKAN GENERASI CEMERLANG BEBAS GIGI BERLUBANG Mohammad Basroni Rizal; Diana Soesilo; Icha Artyas; Noengki Prameswari; Dianty Saptaswari; Cane Lukisari; Budi Handayani; Dwi Andriani; Monika Elidasari; Henu Sumekar; Istien Wardhani; Anindita Apsari; Rizko Wira Artha Megantara
Jurnal Pengabdian Masyarakat Pesisir VOLUME 2 NOMOR 1
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v2i1.90

Introduction. Training of Trainer (ToT) program for teachers and parents for a 21-day morning and evening toothbrush activity teaches children and adults about the benefits of good oral hygiene and shows them how to brush their teeth twice a day with fluoride toothpaste to realizing caries-free Indonesia in 2030 Method. This activity used a digital / online system via the zoom application. Participants' knowledge of dental health was measured through pre-test and post-test questionnaires. Results. The results of the statistical analysis used a paired sample t-test to compare the mean pre-test and post-test scores of the ToT participants showed a significance value (p=0.000). Discussion. There was a significant difference in the level of knowledge of maintaining dental and oral health in teachers and parents of students at Vita School before being given DHE material and after being given DHE through the "Training of Trainer" Conclusion. ToT activities were useful for increasing participants' knowledge about dental health

The effect of low viscous coir fiber resin composite immersion time on composition dissolution Yongki Hadinata Wijaya; Twi Agnita Cevanti; Diana Soesilo; Linda Rochyani
Odonto : Dental Journal Vol 10, No 1 (2023): July 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.1.82-89

Background: E glass fiber dental is a fiber that is often used in dentistry. The availability of E glass fiber in Indonesia is still very limited. Various types of non-dental glass fiber materials are found in the market as engineering materials with relatively cheap prices so that they are expected to be used as an alternative to E glass fiber dental, which one of is coconut fiber. The composition of non-dental glass fiber is almost the same as that of dental E glass fiber. The composition affects the mechanical properties and chemical properties of the fiber. Glass fiber compositions such as Na2O and K2O will increase water resistance. Method: This study was conducted using a post test only control group design. The material used in this study was a composite of coconut coir fiber. The subjects of the coco fiber composite were divided into 4 groups for solubility test based on ISO 4049. The results obtained were analyzed using one-way ANOVA (p = 0.000). The results showed the lowest mean component solubility (%) was in the coconut fiber composite group A (0.476Â±0.03) and the highest yield was in the coco coir fiber composite D (0.6Â±0.01). Results: The results of the one-way ANOVA test showed a significant difference in the coco fiber composite in the solubility of the components (p

Peningkatan Derajat Kesehatan Gigi dan Mulut Siswa SD IT Al Uswah melalui Program Training of Traniner (ToT) Ghita Hadi Hollanda; Diana Soesilo; Aulia Dwi Maharani; Agni Febrina Pargaputri; Ari Rosita Irmawati; Yufita Fitriani; Restika Anindya Pinasti; Bunga Fauzia; Moh. Basroni Rizal
Jurnal Pengabdian Masyarakat Pesisir VOLUME 2 NOMOR 2
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v2i2.95

Kesehatan gigi dan mulut di Indonesia masih merupakan masalah kesehatan yang perlu diperhatikan terutama pada anak-anak usia sekolah dasar. Untuk mengatasi permasalahan kesehatan gigi dan mulut di Indonesia dan menunjang program “Indonesia Bebas Karies 2030”, khususnya pada kelompok masyarakat usia sekolah dan dewasa, Persatuan Dokter Gigi Indonesia (PDGI) dan FDI World Dental Federation akan melakukan kegiatan promotif-preventif. Kegiatan tersebut dinamai “Program Sekolah 21 hari Brush Day and Night”. Program ini dilakukan pada 110 siswa-siswi SD IT Al Uswah Surabaya dengan beberapa kegiatan yang dilakukan diantaranya yaitu edukasi kesehatan gigi dan mulut, sikat gigi bersama, pemeriksaan kesehatan gigi dan mulut serta pemberian topical aplikasi fluor sebagai pencegahan terhadap karies pada gigi. Hasil dari pemeriksaan kesehatan gigi dan mulut menunjukkan bahwa siswa-siswi SD IT Al Uswah Surabaya memiliki indeks def-t kategori tinggi sehingga perlu dilakukan tindakan promotif-preventif dan juga mengadakan training of trainer ke guru dan orang tua agar bisa mengajarkan kebiasaan menjaga kebersihan rongga mulut. Hasil akhir dari program ini diharapkan dapat mencegah meningkatnya derajat kesehatan gigi dan mulut SD IT Al Uswah Surabaya.

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