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All Journal Jurnal Kedokteran Brawijaya Indonesian Journal of Human Nutrition Medical Laboratory Technology Journal Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Journal of Issues in Midwifery Asian Journal of Health Research Jurnal Info Kesehatan
Yahya Irwanto
Department Of Obstetric And Gynecology, Faculty Of Medicine, Universitas Brawijaya, Malang, Indonesia
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Journal : Asian Journal of Health Research

 1 
Effect of Phaleria macrocarpa Extract on NF-KB, MMP-2, and MMP-9 Expression in Endometriosis Mice Model Annissa Febriani; Sutrisno Sutrisno; Yahya Irwanto; Irfani Baihaqi; I Wayan Arsana Wiyasa; Bambang Rahardjo
Asian Journal of Health Research Vol. 1 No. 3 (2022): Volume 1 No 3 (December) 2022
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v1i3.35

Abstract

Introduction: Endometriosis is the most common disease that affects the reproductive health and function of women of reproductive age. Treatment for endometriosis includes surgery and medication. Phaleria macrocarpa is a plant native to Indonesia that contains bioactive fractions known to have antiproliferative and apoptotic effects. Therefore, this study aimed to evaluate the effect of Phaleria macrocarpa extract on matrix metalloproteinases (MMP-2 and MMP-9) and NF-KB expression in the endometriosis mice model (Mus musculus). Methods: This study used a Randomized Post-Test Only with Control Group Design. Involves six groups, namely a negative control group (healthy mice without flavonoids from Phaleria macrocarpa fruit extract), a positive control group (an endometriosis model without being given flavonoids from Phaleria macrocarpa fruit extract), and a treatment group, namely a group given four different doses of flavonoids from Phaleria macrocarpa fruit extract: 3.75 milligrams per day, 7.5 milligrams per day, 11.25 milligrams per day and 15 milligrams per day. expressions of NF-KB, MMP-2, and MMP-9 were seen using Immunohistochemistry staining and observed under a microscope with 40x magnification. The data collection used an immunoratio technique with ImageJ analysis software. Furthermore, data analysis using the one-way ANOVA method. Results: In this study, the treatment group with four different doses of Phaleria macrocarpa extract could reduce the expression of MMP-2, MMP-9, and NF-KB. Groups with higher doses of Phaleria macrocarpa extract showed lessen of MMP-2, MMP-9, and NF-KB expression. Conclusion: In the endometriosis mice model, Phaleria macrocarpa fruit can reduce NF-KB, MMP-2, and MMP-9 expression.
The Effect of Flavonoid Extract from Phaleria macrocarpa to Proliferating Factors (MMP-1, MMP-3, MMP-7) in Endometriosis Mice Model Irwanto, Yahya; Wiyono , Teguh; Wardani, Kusuma
Asian Journal of Health Research Vol. 2 No. 3 (2023): Volume 2 No 3 (December) 2023
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v2i3.102

Abstract

Introduction: Endometriosis is an inflammatory disease influenced by estrogen characterized by ectopic growth of the endometrial stroma and glands. Matrix metalloproteinase (MMP) is a proteolytic enzyme that has an important role in the remodeling of the extracellular matrix in normal tissues but also contributes to pathologies such as tumor invasion. The therapy has many drawbacks, including being expensive and in need of herbal therapy as an alternative. Phaleria macrocarpa is a native plant of Indonesia that contains flavonoids. This study aimed to evaluate the effect of Phaleria macrocarpa flavonoid isolate on the development of apoptosis, proliferation, and angiogenesis in mice model endometriosis. Material and Methods: This research is a true experimental study with a Randomized Post-Test Only with a Control Group in the laboratory. Samples are divided into six groups, a control group and an intervention group, administered with flavonoid extract from Phaleria macrocarpa. Data analysis was carried out by using the Independent T-Test with SPSS for Windows 19.0 software. Results: Each control and intervention group consisted of 6 mice. The normality test for each variable shows p> 0.05. Administration of flavonoid in each group shows a significant decrease in MMP-1 (20.4 ± 7.74 vs 65.68 ± 10.97, p=0.000), MMP-3 (53.34 ± 9.66 vs67.47 ± 10.05, p=0.000), and MMP-7 expression (40.52 ± 5.43 vs 54.13 ± 4.08, p=0.000). Conclusion: Flavonoids from the Phaleria macrocarpa fruit extract were able to reduce the expression of MMP-1, MMP-3, and MMP-7 in mice model endometriosis.
The Effect of Curcumin Administration on p53 and Caspase-3 Expression in Cervical Cancer HeLa Cell Culture Purba, Irene Silvia; Irwanto, Yahya; Rahardjo, Bambang; Handayani, Puspita
Asian Journal of Health Research Vol. 3 No. 3 (2024): Volume 3 No 3 (December) 2024
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v3i3.201

Abstract

Introduction: Cervical cancer is a type of cancer with 605,000 new cases globally each year, with a mortality rate of 340,000. Curcuma longa, or curcumin, has the potential to enhance apoptosis in cervical cancer cells through the NF-kB-p53-caspase-3 pathway. Material and Methods: This in vitro experimental study employed a Completely Randomized Design (CRD) using HeLa cell cultures derived from cervical adenocarcinoma (ATCC® CCL2™). Cells were grown in Dulbecco's Modified Eagle Medium (DMEM) and treated with curcumin at concentrations of 25 µg/mL, 50 µg/mL, 100 µg/mL, 150 µg/mL, and 250 µg/mL. Cytotoxicity was assessed using the CCK-8 assay, apoptosis via flow cytometry, and p53 and caspase-3 expression using immunofluorescence. Statistical analysis was performed with IBM Statistics SPSS 25. Results: There was an increase in the percentage of apoptosis, p53 expression, and caspase-3 expression. At a dose of 100 µg/mL, total apoptosis increased significantly, reaching nearly 25% (p=0.000). P53 expression significantly increased at curcumin doses of 50 µg/mL and 100 µg/mL (p=0.021). There was a significant increase in caspase-3 expression, reaching approximately 86.2%, with a maximum effect at a dose of 100 µg/mL (p=0.002). Conclusion: Curcumin shows promising potential as an anticancer agent by reducing cell viability and enhancing apoptotic activity in HeLa cervical cancer cells.
Neoadjuvant Chemotherapy Before Radical Hysterectomy in Cervical Cancer: A Systematic Comparison of Robotic vs Abdominal Approaches Wahyuni, Rizki; Irwanto, Yahya
Asian Journal of Health Research Vol. 4 No. 3 (2025): Volume 4 No 3 (December) 2025
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v4i3.248

Abstract

Introduction: Cervical cancer remains a leading cause of cancer mortality worldwide. Neoadjuvant chemotherapy (NACT) followed by radical hysterectomy (RH) is increasingly used, but the comparative safety and efficacy of robotic‐assisted versus open abdominal RH after NACT remain unclear.    Material and Methods: This PRISMA‐compliant systematic review (January 2015–March 2025) analyzed randomized and observational studies comparing robotic‐assisted RH (RRH) and open RH (ARH) in women with FIGO IB₂–IIA₂ cervical cancer treated with platinum‐based NACT. The primary endpoints were intraoperative and postoperative complications. Seven studies involving 534 patients (284 minimally invasive vs. 250 open) met the inclusion criteria.    Results: Overall complication rates were similar between groups (15–20%). Minimally invasive surgery (MIS) significantly reduced estimated blood loss by 75–315 mL (p<0.05) and shortened hospital stay by 1–2 days (p<0.01). Operative time was comparable, with minor increases in conventional laparoscopy. In nerve‐sparing procedures, MIS provided faster bladder and bowel recovery (post‐void residual normalization 11 vs. 18 days; time to flatus 39 vs. 56 hours; p<0.01) and improved 1‐year urinary and intestinal quality of life.    Conclusion: Six of seven studies found no difference in disease‐free or overall survival between MIS and open RH. One study reported worse outcomes with RRH among patients with FIGO IIB disease and multi‐agent NACT (HR 2.5–3.0; p<0.05), suggesting risk in higher‐stage, heavily pretreated cases. Overall, robotic‐assisted RH appears to be a safe and effective alternative to open surgery for appropriately selected FIGO IB₂–IIA₂ cervical cancer patients after NACT.
Co-Authors A.A. Ketut Agung Cahyawan W Annissa Febriani Bambang Rahardjo Cholili, Muhamad Nofa Dewi, Rose Khasana Diah Ayuningtyas Dwi Wulandari, Recavery Edy Mustofa Fadilah, R. A. Rahmawati Nurul Fatmawati, Fatmawati I Wayan Subage Irfani Baihaqi Kenty Wantri Anita, Kenty Wantri Khasanah, Wasingatin Catur Khoirinda, Ani KUSUMA WARDANI Mudinillah, Adam Nugrahanti Prasetyorini Purba, Irene Silvia Puspita Handayani Rahajeng Rahajeng Rahmi Utami Ratnawati, Putu A.R. Soetomo Soewarto Sutrisno Sutrisno Sutrisno, Sutrisno Tatit Nurseta Tatit Nurseta Wahyuni, Rizki Wijaya, Dyah Ayu Septika Wiyasa, I W.A. Wiyasa, I Wayan Arsana Wiyono , Teguh