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EKSPRESI BCL-2 PADA NEOPLASMA EPITELIAL OVARIUM JINAK, BORDERLINE, DAN GANAS SERTA KORELASINYA DENGAN DERAJAT HISTOPATOLOGIK Dewi, Rose Khasana; Hardika, Arif Satria; Retnani, Diah Prabawati; Rasyid, Harun Al
Majalah Kesehatan FKUB Vol 7, No 1 (2020): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Neoplasma epitelial ovarium diklasifikasikan menjadi 3 kategori yaitu jinak, borderline, dan ganas. Neoplasma ganas ovarium termasuk dalam sepuluh besar penyebab kematian tersering akibat kanker pada perempuan di dunia. Penentuan kategori neoplasma ovarium kadang masih menjadi masalah karena dipengaruhi oleh beberapa faktor, termasuk proliferasi dan apoptosis sel serta invasi stroma. Bcl-2 (B-cell lymphoma 2) merupakan salah satu protein prosurvival yang berfungsi sebagai antiapoptosis dan cukup berperan dalam perkembangan neoplasma. Tujuan penelitian ini adalah untuk mengetahui perbedaan eks-presi Bcl-2 pada neoplasma epitelial ovarium jinak, borderline, ganas serta untuk mengungkap hubungan ekspresi Bcl-2 dengan derajat histopatologik pada neoplasma ganas. Penelitian ini merupakan penelitian analitik cross-sectional. Sampel penelitian berupa 35 blok parafin kasus neoplasma ovarium serosum dan musinosum (13 jinak, 8 borderline, 14 ganas) di RSUD Dr. Saiful Anwar Malang periode 2016-2017. Kelompok neoplasma ganas terdiri dari masing-masing 5 kasus derajat 1 dan 3 (35,7%) serta 4 kasus derajat 2 (28,6%). Perbedaan ekspresi pulasan imunohistokimia Bcl-2 antara neoplasma jinak, borderline, dan ganas dianalisis menggunakan uji Kruskal-Wallis, sedangkan korelasinya dengan derajat histopatologik dianalisis menggunakan uji korelasi Spearman. Hasil penelitian menunjukkan ekspresi Bcl-2 tidak berbeda signifikan antara neoplasma jinak, borderline, dan ganas (p = 0,159). Bcl-2 pada neoplasma ganas menunjukkan eks-presi yang semakin rendah dengan meningkatnya derajat histopatologik (p = 0,021 dan r = -0,608). Ke-simpulannya, tidak terdapat perbedaan ekspresi Bcl-2 yang signifikan antara neoplasma epitelial ovarium jinak, borderline, dan ganas. Ekspresi Bcl-2 berkorelasi negatif yang kuat dengan derajat histopatologik pada neoplasma ganas.Â

Differences in p53 Expression in High-Grade Conventional Osteosarcoma and Giant Cell Tumor of Bone Teguh Prihantoro; Eviana Norahmawati; Rose Khasana Dewi; Holipah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 15 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i15.680

Background: Osteosarcoma is the most common primary tumor of bone. Generally, patients with osteosarcoma have a poor prognosis. So it is very important to be able to diagnose as early as possible. Histopathologically, osteosarcoma has a variety of types similar to non-malignant lesions, such as giant cell tumor of bone. Therefore, caution is needed in diagnosing because if it is misdiagnosed, it will result in different management. This study aims to look at differences in p53 protein expression in high-grade conventional osteosarcoma and giant cell tumor of bone. Methods: This observational analytic study used 30 samples from tissue biopsies/surgeries diagnosed with conventional high-grade osteosarcoma and 30 samples of giant cell tumor of bone at the Anatomical Pathology Installation of Dr. Saiful Anwar General Hospital Malang, Indonesia, between 2018-2022. p53 expression was examined using the immunohistochemical staining method. Positive if stained with brown on the nucleus of the cell, the percentage of 500 malignant cells was calculated in the large field of view of the objective lens (x40) randomly. Univariate and bivariate data analysis was performed using SPSS. Results: The average p53 protein expression in conventional high-grade osteosarcoma was 80.0% ± 26.2, with the lowest value being 19% and the highest value being 100%. Whereas in giant cell tumor of bone, the average p53 expression was 1.7% ± 4.3, with the lowest value being 0% and the highest value being 17.6%. Statistical analysis using the Mann-Whitney test showed that p53 expression in conventional high-grade osteosarcoma and giant cell tumor of bone showed a significant difference (p=0.000). Conclusion: There is a significant difference between p53 expression in high-grade conventional osteosarcoma and giant cell tumor of bone.

Differences in p53 Expression in High-Grade Conventional Osteosarcoma and Giant Cell Tumor of Bone Teguh Prihantoro; Eviana Norahmawati; Rose Khasana Dewi; Holipah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 15 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i15.680

Background: Osteosarcoma is the most common primary tumor of bone. Generally, patients with osteosarcoma have a poor prognosis. So it is very important to be able to diagnose as early as possible. Histopathologically, osteosarcoma has a variety of types similar to non-malignant lesions, such as giant cell tumor of bone. Therefore, caution is needed in diagnosing because if it is misdiagnosed, it will result in different management. This study aims to look at differences in p53 protein expression in high-grade conventional osteosarcoma and giant cell tumor of bone. Methods: This observational analytic study used 30 samples from tissue biopsies/surgeries diagnosed with conventional high-grade osteosarcoma and 30 samples of giant cell tumor of bone at the Anatomical Pathology Installation of Dr. Saiful Anwar General Hospital Malang, Indonesia, between 2018-2022. p53 expression was examined using the immunohistochemical staining method. Positive if stained with brown on the nucleus of the cell, the percentage of 500 malignant cells was calculated in the large field of view of the objective lens (x40) randomly. Univariate and bivariate data analysis was performed using SPSS. Results: The average p53 protein expression in conventional high-grade osteosarcoma was 80.0% ± 26.2, with the lowest value being 19% and the highest value being 100%. Whereas in giant cell tumor of bone, the average p53 expression was 1.7% ± 4.3, with the lowest value being 0% and the highest value being 17.6%. Statistical analysis using the Mann-Whitney test showed that p53 expression in conventional high-grade osteosarcoma and giant cell tumor of bone showed a significant difference (p=0.000). Conclusion: There is a significant difference between p53 expression in high-grade conventional osteosarcoma and giant cell tumor of bone.

HUBUNGAN ANTARA EKSPRESI CYCLIN D1 DAN SMOOTH MUSCLE ACTIN DENGAN KARSINOMA SEL BASAL KELOMPOK RISIKO REKURENSI RENDAH DAN TINGGI Berlian Anggraeni Putri; Diah Prabawati Retnani; Hendy Setyo Yudhanto; Holipah; Rose Khasana Dewi; Hery Susilo
Majalah Kesehatan FKUB Vol. 9 No. 2 (2022): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/majalahkesehatan.2022.009.02.1

Kanker kulit yang tersering dikelompokkan menjadi kanker kulit melanositik dan nonmelanositik. Kanker kulit nonmelanositik menempati urutan kelima terbanyak dari seluruh jenis kanker di dunia dengan jenis yang paling sering adalah karsinoma sel basal. Secara histopatologis karsinoma sel basal dibagi menjadi dua kelompok, yaitu kelompok risiko rekurensi rendah dan tinggi. Sifat Karsinoma sel basal yang proliferatif dan invasif membutuhkan marker yang dapat memprediksi keduanya, di antaranya adalah cyclin D1 dan smooth muscle actin (SMA). Penelitian ini untuk membuktikan adanya hubungan antara ekspresi cyclin D1 dengan karsinoma sel basal kelompok risiko rendah dan tinggi. Desain penelitian adalah observasional analitik dengan metode cross-sectional, sampel penelitian karsinoma sel basal ditetapkan sebanyak 30 sampel, terdiri dari 15 sampel kelompok risiko rekurensi rendah dan 15 sampel kelompok risiko rekurensi tinggi. Ekspresi cyclin D1 dan SMA pada sediaan imunohistokimia dinilai secara semi kuantitatif pada masing-masing kelompok kemudian akan dilakukan uji chi-square. Hasil penelitian ini menunjukkan bahwa ekspresi cyclin D1 memiliki hubungan dengan karsinoma sel basal kelompok risiko rekurensi rendah dan tinggi (p = 0,008 < 0,05), namun tidak berhubungan dengan ekspresi SMA (p = 0,389 > 0,05). Ekspresi cyclin D1 pada karsinoma sel basal kelompok risiko rendah memiliki skor yang lebih rendah dibandingkan dengan kelompok risiko rekurensi tinggi. Sementara pada SMA tidak didapatkan perbedaan yang bermakna. Kesimpulan penelitian ini, ekspresi cyclin D1 memiliki hubungan dengan karsinoma sel basal kelompok risiko rekurensi rendah dan tinggi, sedangkan SMA tidak berhubungan.

Profil Klinikopatologi Meningioma di Instalasi Patologi Anatomi RSUD Dr. Saiful Anwar Malang Periode Tahun 2016-2020 Putri, Anggie Sasmita Kharisma; Diah Prabawati; Dewi, Rose Khasana; Angelina, Aina
Jurnal Klinik dan Riset Kesehatan Vol 2 No 3 (2023): Edisi Juni
Publisher : RSUD Dr. Saiful Anwar Province of East Java

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jk-risk.02.3.3

Background. The most common intracranial neoplasms worldwide is meningioma. It has been classified into three grades by World Health Organization (WHO) in which each grade has several histological variants. The prognosis of meningioma is affected by many factors including histopathological type of tumor, age, and gender. Objective. The aim of this study is to determine the frequency of meningioma based on histopathological type, age, and gender from 2016 to 2020 in Saiful Anwar General Hospital Malang. Methods. This study was a quantitative descriptive study and data was collected from medical records from 2016 to 2020 in Department of Anatomic Pathology, Medical Faculty, Brawijaya University, Saiful Anwar General Hospital Malang. Histopathological type of meningioma, age, and gender were acquired. Results and Discussion. From 2016 to 2020, there were 96 eligible cases of meningioma. Ratio of men to women was 1:12.7. Meningioma was most commonly found in age group 4150 years old (43.75%). The most common histopathological type of meningioma was meningotheliomatous meningioma (37.5%). The average of meningioma cases was 19 per year. In this study, there was an increase in incidence rate of meningioma in 2017, and decreased in 2020. It may happen because The government has a better referral system to provide health care, thus meningioma can be detected earlier. Conclusion. Frequency of meningioma is higher in women than men, and increases with age until 50 years, which then decreases. Meningotheliomatous meningioma is the most common histopathological type in this study.

EFEK PEMBERIAN THYMOQUINONE TERHADAP JUMLAH SEL MAKROFAG PADA PARU TIKUS YANG DIINFEKSI BAKTERI Mycobacterium tuberculosis Angelina, Aina; Iskandar, Agustin; Rambe, Annisa Fadhila Aurelia; Kusuma, Ihda Dian; Dewi, Rose Khasana; Olivianto, Ery
Majalah Kesehatan Vol. 11 No. 3 (2024): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/majalahkesehatan.2024.011.03.1

Penyakit tuberkulosis merupakan salah satu penyebab kematian terbanyak di dunia. Hal ini karena banyaknya kasus resistensi OAT. Imunomodulator dapat meningkatan sistem imun dan membantu pengobatan tuberkulosis. Thymoquinone adalah salah satu komponen aktif dari jintan hitam yang dapat digunakan sebagai imunomodulator. Penelitian ini bertujuan mengetahui efek thymoquinone terhadap jumlah sel makrofag pada paru tikus (Rattus norvegicus) model tuberkulosis yang diamati secara mikroskopis. Tikus dibagi menjadi sepuluh kelompok yaitu lima kelompok perlakuan 14 hari dan lima kelompok perlakuan 21 hari. Tikus diinokulasi oleh bakteri Mycobacterium tuberculosis secara intra-trakeal. Thymoquinone diberikan per oral pada kelompok perlakuan dengan tiga dosis berbeda (25 µg/kgBB, 50 µg/kgBB, dan 75 µg/kgBB). Pengamatan jumlah sel makrofag dilakukan dengan pulasan imunohistokimia menggunakan antibodi CD68. Hasil penelitian ini menunjukkan bahwa pemberian thymoquinone pada dosis 50 µg/kgBB dapat meningkatkan jumlah sel makrofag pada paru tikus model TB. Hasil analisis oneway ANOVA menunjukkan perbedaan signifikan pada kelompok perlakuan 21 hari (p = 0,007;α < 0,05) sedangkan pada kelompok perlakuan 14 hari tidak didapatkan perbedaan signifikan. Kesimpulan penelitian ini ialah pemberian thymoquinone selama 21 hari dapat meningkatkan jumlah sel makrofag pada paru tikus yang diinokulasi bakteri Mycobacterium tuberculosis.

SARM Rad140 Increases Osteoblasts, Muscle Fiber Size, Myonuclei, and Reduces Osteoclasts in Orchidectomized Wistar Rats Budaya, Taufiq Nur; Daryanto, Besut; Seputra, Kurnia Penta; Fabrianta, Dio Mafazi; Ekaputra, Aditya Airlangga; Dewi, RA Rose Khasana; Anita, Kenty Wantri; Dhani, Fauzan Kurniawan; Rofifa, Amira Fithri
Molecular and Cellular Biomedical Sciences Vol 9, No 1 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i1.536

Background: Orchidectomy is a surgical androgen deprivation therapy (ADT) for prostate cancer patients to achieve castrate testosterone levels. Selective androgen receptor modulators (SARMs) are used to mitigate the adverse effects of ADT, including elevated risk of osteoporosis, and reduced skeletal muscle mass. Rad140 is a novel SARMs that has high affinity for the androgen receptors. This study was conducted to determine the effects of SARM Rad140 on the number of osteoblasts, osteoclasts, muscle fiber cross-sectional area (CSA), muscle size, and number of myonuclei in rats underwent orchidectomy.Materials and Methods: An experimental study was conducted using a randomized post-test only control group design. Following orchidectomy, SARM Rad140 was administered orally for six weeks at various doses. Osteoblasts, osteoclasts, muscle fiber CSA, muscle size, and number of myonuclei were measured. Quantitative analysis was performed using one-way ANOVA.Results: There were significant differences in the effects of SARM Rad140 at different doses on osteoblast and osteoclast cells. At higher doses, the osteoblast cell counts in rats tended to increase, while the osteoclast counts tended to decline. The treatment group also showed significant results in the CSA of the gastrocnemius muscle fibers, as well as in the number of myonuclei of the gastrocnemius muscle.Conclusion: SARM Rad140 significantly increased the number of osteoblasts, muscle fiber CSA, and gastrocnemius muscle myonuclei, while decreasing osteoclasts. SARM Rad140 is a promising therapy for osteoporosis and muscle weakening due to ADT.Keywords: SARM Rad140, orchidectomy, osteoblasts, osteoclasts, muscle fiber cross sectional area, myonuclei

The expression of immunohistochemical biomarkers PAX8 and CD117 in platinum resistant ovarian cancer at Saiful Anwar General Hospital, Malang, Indonesia Widodo, Robby Rinaldi; Nurseta , Tatit; Mustofa, Edy; Arianto, Onni Dwi; Dewi, R. A. Rose Khasana
Majalah Obstetri & Ginekologi Vol. 33 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V33I12025.1-10

HIGHLIGHTS Ovarian cancer, a prevalent gynecologic malignancy affecting women, poses a significant challenge due to the development of resistance to platinum-based therapy in its management. PAX8 and CD117 have demonstrated potential as predictive biomarkers for platinum resistance in ovarian cancer; however, studies exploring their immunohistochemical expression in relation to platinum resistance in ovarian cancer patients remain limited. This study aims to evaluate the immunohistochemical expression of CD117 and PAX8 in ovarian cancer tissues and investigate their association with the emergence of platinum resistance. ABSTRACT Objective: Ovarian cancer, a prevalent gynecologic malignancy, frequently presents challenges due to platinum therapy resistance. This study aims to investigate a monitoring approach for post-operative ovarian carcinoma and evaluate the immunohistochemical expression of PAX8 and CD117 in ovarian cancer tissues, assessing their association with platinum resistance development. Materials and Methods: Employing a cross-sectional observational analytical design, this study utilized consecutive sampling of patients meeting predefined inclusion and exclusion criteria. Tissue samples, obtained from biopsy or surgical procedures and processed into representative paraffin blocks, underwent immunohistochemical analysis at the Anatomical Pathology Department of Saiful Anwar Hospital, Malang, Indonesia. Expressions of PAX8 and CD117 were evaluated using immunohistochemistry. Diagnostic performance was assessed through receiver operating characteristic (ROC) curves and Youden index calculations to determine sensitivity and specificity. Results: The study findings revealed that the area under the curve (AUC) for PAX8 and CD117 was 0.785 and 0.809, respectively. PAX8 expression exhibited a positive predictive value of 53.125%, negative predictive value of 87.50%, sensitivity of 18.75%, specificity of 51.85%, and accuracy of 60.0%. For CD117 expression, the corresponding values were 71.4%, 72.73%, 70%, 72.73%, and 70% for positive predictive value, negative predictive value, sensitivity, specificity, and accuracy, respectively. Conclusion: The immunohistochemical expression of PAX8 and CD117 in ovarian cancer tissues may serve as prognostic biomarkers for platinum resistance. Despite these findings, the study acknowledges several limitations that warrant refinement in future research.

Profil Klinikopatologi Meningioma di Instalasi Patologi Anatomi RSUD Dr. Saiful Anwar Malang Periode Tahun 2016-2020 Putri, Anggie Sasmita Kharisma; Retnani, Diah Prabawati; Dewi, Rose Khasana; Angelina, Aina; Diah Prabawati
Jurnal Klinik dan Riset Kesehatan Vol 2 No 3 (2023): Edisi Juni
Publisher : RSUD Dr. Saiful Anwar Province of East Java

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jk-risk.02.3.3

Background. The most common intracranial neoplasms worldwide is meningioma. It has been classified into three grades by World Health Organization (WHO) in which each grade has several histological variants. The prognosis of meningioma is affected by many factors including histopathological type of tumor, age, and gender. Objective. The aim of this study is to determine the frequency of meningioma based on histopathological type, age, and gender from 2016 to 2020 in Saiful Anwar General Hospital Malang. Methods. This study was a quantitative descriptive study and data was collected from medical records from 2016 to 2020 in Department of Anatomic Pathology, Medical Faculty, Brawijaya University, Saiful Anwar General Hospital Malang. Histopathological type of meningioma, age, and gender were acquired. Results and Discussion. From 2016 to 2020, there were 96 eligible cases of meningioma. Ratio of men to women was 1:12.7. Meningioma was most commonly found in age group 4150 years old (43.75%). The most common histopathological type of meningioma was meningotheliomatous meningioma (37.5%). The average of meningioma cases was 19 per year. In this study, there was an increase in incidence rate of meningioma in 2017, and decreased in 2020. It may happen because The government has a better referral system to provide health care, thus meningioma can be detected earlier. Conclusion. Frequency of meningioma is higher in women than men, and increases with age until 50 years, which then decreases. Meningotheliomatous meningioma is the most common histopathological type in this study.

The Effect Flavonoids Phaleria macrocarpa Fruit Extract on Thickness of Trabeculae, Cortex Ratio Femoral Bone and Aortic Intima-Media in Mice Menopause Model Fadilah, R. A. Rahmawati Nurul; Khoirinda, Ani; Sutrisno, Sutrisno; Irwanto, Yahya; Anita, Kenty Wantri; Dewi, R. A. Rose Khasana
JURNAL INFO KESEHATAN Vol 22 No 2 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss2.1553

A deficiency of the hormone estrogen at menopause can lead to an increased rate of the destruction of the bone tissue that leads to bone loss, which can lead to osteoporosis and impaired fat metabolism, which increases the risk of atherosclerosis. Phytoestrogens from flavonoid extract P. Macrocarpa, having effects similar to endogenous estrogens themselves, prevent osteoporosis and atherosclerosis in menopausal women. The purpose of this research is to assess the influence of flavonoids from P. Macrocarpa fruit extract on trabeculae cortex thickness, ratio of femoral bone, and aortic IMT (A-IMT) in a menopausal mouse model. The study was conducted in a true experimental-posttest-only control group design. Using 32 mice; namely KN (normal mice with no treatment), KP (OVX with no treatment), P1(OVX and given flavonoid 3.75 mg/mice/day), P2 (OVX and given flavonoid 7.5 mg/mice/day), P3(OVX and given flavonoid 11.25 mg/mice/day), P4 (OVX and given flavonoid 15 mg/mice/day), the treatment given within 14 days. Then the thickness of the trabeculae, cortex, and intima-media aorta with Hematoxylin-Eosin (HE) staining. In the trabeculae, cortex thickness ratio obtained KN results meaningfully dissimilar to the KP group and the P3 and P4 groups were meaningfully dissimilar from the KP. The A-BMI in KP is meaningfully dissimilar to P1, P2, P3 and P4. The conclusion of the study is flavonoid fruit extract P. Macrocarpa can increase the thickness ratio of trabeculae, and cortex femoral bone of mice menopausal model in groups P3, P4 and can decrease A-IMT starting in groups P1 to P4.

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