Garuda - Garba Rujukan Digital

Expression of Fas Ligand is Higher in Early Stage Cervical Cancer with Lymph Nodes Metastasis Irwanto, Yahya
Indonesian Journal of Obstetrics and Gynecology Volume. 36, No. 4, October 2012
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (239.225 KB)

Objective: To know whether the expression of Fas Ligand has correlation with incidene of metastasis of pelvic lymph node and lymph-vascular stromal invasion (LVSI). Methods: All patients diagnosed of cervical cancer stage IB or IIA who underwent radical hysterectomy in Dr. Cipto Mangunkusumo Central General Hospital from January 2008 until December 2009 were included in analytic cross sectional study. We tested expression of Fas Ligand in cervical cancer specimen by immunohystochemistry with monoclonal antibody. The expression of Fas Ligand was compared between the group of patients with a positive and negative pelvic lymph node and between LVSI positive and negative. The difference of expression in both group were statistically analized with Chi-square test and the correlation Spearman test. Result: Ninety one patients underwent radical hysterectomy for two years and 43 patients were included in these study. The expression of Fas Ligand in 7 patients (16.3%) were negative and the others were positive, with weak, moderate and strong expression were 4 (9.3%), 27 (62.8%), and 5 (11.6%) respectively. The expression of Fas Ligand was significantly higher in the group of patients with positive pelvic lymph node compared to the group of the patients with negative pelvic lymph node (p=0.007) but there was no significant difference between group of the patients with LVSI positive and negative. With Spearman test, we found that the expression of Fas Ligand had a strong correlation with pelvic lymph nodes metastasis (coeff. correlation=0.519 and p=0.00) and have no correlation with LVSI (coef. corellation=0.112 and p=0.474). Conclusion: These finding suggested that expression of Fas Ligand in cervical cancer patients has a strong correlation with the incidence of pelvic lymph node metastasis and none with LVSI. [Indones J Obstet Gynecol 2012; 36-4: 194-9] Keywords: cervical cancer, fas ligand, immunohystochemistry, LVSI, pelvic lymph node metastasis

Curcumin Administration on Proliferation and Apoptosis Index in Complete Hydatidiform Mole CellCulture Nurseta, Tatit; Irwanto, Yahya; Imelda, Imelda
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 1, January 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To investigate that curcumin can decreasing proliferation index and increasing apoptosis index. Method: This is an experimental non-blinded study with post test control group design, at Cell/Tissue Culture Laboratory at Medical Faculty of Brawijaya University. This study using CHM trophoblastic cell culture from CHM curettage patient, exposed by several doses of curcumin, 0, 50, 100, 200, 400 and 800 μM, then examined by the method of MTT proliferation index and apoptosis index by the method of labeling DNA fragmentation TUNEL system. Data analyzed by one-way ANOVA. Result: In this study, the mean values obtained decrease in proliferation index with increasing doses of curcumin. Giving a dose of 200 μM curcumin, 400 μM and 800 μM proved highly significant (p = 0.001) reduced proliferation index compared with the control and curcumin dose of 50 μM and 100 μM. From this research shows that there are significant differences in the increase of apoptosis index (p = 0.001) between the control group with curcumin dose group 200 μM, 400 μM and 800 μM. But there was no significant difference in the mean of apoptosis index among the three dose groups. Conclusion: Giving curcumin dose of 200 μM can decreasing the proliferation index and increasing the apoptosis index increases in CHM trophoblastic cell culture. [Indones J Obstet Gynecol 2016; 1: 37-41] Keywords: apoptosis index, curcumin, proliferation index

Curcumin Administration on Proliferation and Apoptosis Index in Complete Hydatidiform Mole CellCulture Nurseta, Tatit; Irwanto, Yahya; Imelda, Imelda
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 1, January 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To investigate that curcumin can decreasing proliferation index and increasing apoptosis index. Method: This is an experimental non-blinded study with post test control group design, at Cell/Tissue Culture Laboratory at Medical Faculty of Brawijaya University. This study using CHM trophoblastic cell culture from CHM curettage patient, exposed by several doses of curcumin, 0, 50, 100, 200, 400 and 800 μM, then examined by the method of MTT proliferation index and apoptosis index by the method of labeling DNA fragmentation TUNEL system. Data analyzed by one-way ANOVA. Result: In this study, the mean values obtained decrease in proliferation index with increasing doses of curcumin. Giving a dose of 200 μM curcumin, 400 μM and 800 μM proved highly significant (p = 0.001) reduced proliferation index compared with the control and curcumin dose of 50 μM and 100 μM. From this research shows that there are significant differences in the increase of apoptosis index (p = 0.001) between the control group with curcumin dose group 200 μM, 400 μM and 800 μM. But there was no significant difference in the mean of apoptosis index among the three dose groups. Conclusion: Giving curcumin dose of 200 μM can decreasing the proliferation index and increasing the apoptosis index increases in CHM trophoblastic cell culture. [Indones J Obstet Gynecol 2016; 1: 37-41] Keywords: apoptosis index, curcumin, proliferation index

Expression of Fas Ligand is Higher in Early Stage Cervical Cancer with Lymph Nodes Metastasis Irwanto, Yahya
Indonesian Journal of Obstetrics and Gynecology Volume. 36, No. 4, October 2012
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To know whether the expression of Fas Ligand has correlation with incidene of metastasis of pelvic lymph node and lymph-vascular stromal invasion (LVSI). Methods: All patients diagnosed of cervical cancer stage IB or IIA who underwent radical hysterectomy in Dr. Cipto Mangunkusumo Central General Hospital from January 2008 until December 2009 were included in analytic cross sectional study. We tested expression of Fas Ligand in cervical cancer specimen by immunohystochemistry with monoclonal antibody. The expression of Fas Ligand was compared between the group of patients with a positive and negative pelvic lymph node and between LVSI positive and negative. The difference of expression in both group were statistically analized with Chi-square test and the correlation Spearman test. Result: Ninety one patients underwent radical hysterectomy for two years and 43 patients were included in these study. The expression of Fas Ligand in 7 patients (16.3%) were negative and the others were positive, with weak, moderate and strong expression were 4 (9.3%), 27 (62.8%), and 5 (11.6%) respectively. The expression of Fas Ligand was significantly higher in the group of patients with positive pelvic lymph node compared to the group of the patients with negative pelvic lymph node (p=0.007) but there was no significant difference between group of the patients with LVSI positive and negative. With Spearman test, we found that the expression of Fas Ligand had a strong correlation with pelvic lymph nodes metastasis (coeff. correlation=0.519 and p=0.00) and have no correlation with LVSI (coef. corellation=0.112 and p=0.474). Conclusion: These finding suggested that expression of Fas Ligand in cervical cancer patients has a strong correlation with the incidence of pelvic lymph node metastasis and none with LVSI. [Indones J Obstet Gynecol 2012; 36-4: 194-9] Keywords: cervical cancer, fas ligand, immunohystochemistry, LVSI, pelvic lymph node metastasis

Perbedaan Disfungsi Sexual Wanita yang Melahirkan Secara Pervaginam dengan Episiotomy Mediolateral dan Seksio Sesarea Irwanto, Yahya; Mustofa, Edy
Journal of Issues in Midwifery Vol 2, No 3 (2018)
Publisher : Journal of Issues in Midwifery

Generally, the causes of Postpartum Female Sexual Dysfunction (PPFSD) are dyspareunia, perineal pain, the discomfort of incision lesion including surgery, reduction of libido, lack of lubrication in the vagina, changes in seeing body image to be negative, and anorgasm irelated to pain and trauma. Perineal pain and dyspareunia are post partum problems which occur frequently and interfere with normal sexual function, that usually are caused by perineal trauma, episiotomy, and child birth instrumentation.This study was conducted by a cross-sectional study. Research subjects consisted of women post vaginal delivery with mediolateral episiotomy and postcesarean section. Each group consisted of 45 people. After three months post partum sexual function as sessed using FSFI scores (Female Sexual Function Index) as well as an assess ment of demographic data include age, religion, education, employment, insurance coverage/BPJS, gestational age, birthweight, stitches condition, medical treatment, nursing, assistance of baby sitter and problems in the family. The data had to fulfill the inclusion and exclusion criteria in the form of question naires were further tested withc hi-square analysis and independent t-test.In the demographic data analysis withc hi-squareanalys is didn’t find any significant differences between the two groups, with p>0.05. The sexual dysfunction among women after vaginal delivery with mediolateral episiotomy and cesarean section there was no significant difference p=0.081, and p>0.05. On the FSFI scores domains there are significant differences between the two group son the variables of sexual desire, orgasm, pain and FSFI total scores.

PENGARUH PEMBERIAN KAPSUL EKSTRAK GARLIK TERHADAP PERUBAHAN TEKANAN DARAH DAN JUMLAH TROMBOSIT PADA PENDERITA PREEKLAMPSI RINGAN Irwanto, Yahya; Wahyudi, Imam; Soewarto, Soetomo
Jurnal Kedokteran Brawijaya Vol 20, No 3 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

The objectives is to investigate the efectivity of Â extract garlic capsule compared to standart therapytoward the changes of bloodÂ presure and thrombocyt level in the mild-preeclampsy patients. This study was a single-blind randomised clinical trial. The subject ofÂ study were the mild-preeclampsy pregnant women within 20 â€“ 36 weeks of gestational age whose came to the Poli Hamil RSUD dr.Â Saiful Anwar. The subject divided into 2 groups, the treatment group received both of the standart therapy of mild-preeclampsy andÂ extract garlic capsule, while the controlling grouponly received the standart therapy of mild-preeclampsy. After 2 weeks, the bloodÂ pressure and the trombocyt count was evaluated. Thesystolic blood pressure of the treatment group wasdecrease at average ofÂ 10.12 mmHg, from 140.25 Â± 5.57 before treatment to 130.12 Â± 10.84 mmHg after treatment, while in thecontrololling group thereÂ was an increase at average of 5.56 mmHg from 140.44Â± 6.14 mmHg to 146Â±13.81 mmHg. The T Test and ANCOVA analyzingÂ showed a significant result (P value were 0.001 and0.0008).

Risk of Malignancy Index 4 (RMI4) and Risk of Malignancy Index 3 (RMI3) as Diagnostic Tests for Adnexal Tumor: Risk of Malignancy Index 4 (RMI4) dan Risk of Malignancy Index 3 (RMI3) sebagai Alat Diagnostik untuk Tumor Adneksa Tatit Nurseta; Yahya Irwanto; Nugrahanti Prasetyorini; Bambang Rahardjo; I Wayan Subage
Indonesian Journal of Obstetrics and Gynecology Volume 8 No. 4 October 2020
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32771/inajog.v8i4.1342

Objective: Comparing scoring with RMI3 and RMI4 in establishing the diagnosis of adnexal tumour in RSUD Dr. Saiful Anwar Malang.Methods: Prospective cohort study with samples of all patients suspected of having an adnexal tumour diagnosed in gynecology policlinic using gynecological oncology policlinic medical records at RSUD Dr. Saiful Anwar in the form of age, demographics, menopause status, Ca125, ultrasound results.Results: Between the RMI3 results and the results of histopathology, a contingency coeffi cient of 0.596 was obtained with a p-value of 0.000015 (p <0.05), with a PPV of 83%, an NPV of 91%. Between the RMI4 results and the histopathology results, a contingency coeffi cient of 0.657 with a p-value of 0.0000004 (p <0.05) was obtained, with a PPV of 92%, NPV of 95%. On the ROC curve, the area of the predicted results using the RMI4 score is higher than the RMI3 score.Conclusions: Using the RMI4 score results in more accurate predictions than the RMI3 score in detecting adnexal tumour malignancies.Keywords: adnexal tumours, diagnostic tests, ovarian tumours, risk of malignancy index, ultrasound. Abstrak Tujuan: Membandingkan Skoring RMI3 dan RMI4 dalam menegakkan diagnosis tumor adneksa di RSUD dr. Saiful Anwar Malang.Metode: penelitian kohort prospective dengan sampel semua pasien yang dicurigai menderita tumor adneksa yang didiagnosis di poli Ginekologi menggunakan data rekam medis poli Ginekologi Onkologi RSUD dr. Saiful Anwar berupa usia, demografi , status menopause, Ca125, hasil USG.Hasil: Antara hasil RMI3 dengan hasil hispatologi, didapatkan koefi sien kontingensi sebesar 0,596 dengan p-value 0,000015 (p<0,05), dengan PPV 83%, NPV 91%. Antara hasil RMI4 dengan hasil histopatologi, didapatkan koefisien kontingensi sebesar 0,657 dengan p-value 0,0000004 (p<0,05), dengan PPV 92%, NPV 95%. Pada kurva ROC, luas area hasil prediksi dengan menggunakan skor RMI4 lebih tinggi daripada skor RMI3.Kesimpulan: Menggunakan skor RMI4 menghasilkan prediksi yang lebih tepat daripada skor RMI3 dalam mendeteksi keganasan tumor adneksa.Kata kunci: risk of malignancy index, tumor adneksa, tumor ovarium, uji diagnostik, USG.

Pengaruh Pemberian Ekstrak Etanol Biji Pepaya (Carica papaya L.) terhadap Jumlah Folikel Preantral, Antral dan Berat Ovarium Tikus Putih Betina (Rattus norvegicus) Galur Wistar Diah Ayuningtyas; Kenty Wantri Anita; Yahya Irwanto
Journal of Issues in Midwifery Vol. 6 No. 2 (2022): Vol. 6 No. 2
Publisher : Journal of Issues in Midwifery

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.JOIM.2022.006.02.1

Biji Pepaya (Carica papaya L.) merupakan salah satu bagian tanaman pepaya yang digunakan sebagai obat tradisional. Bahan aktif yang terdapat pada biji pepaya ini dapat mempengaruhi hormon yang dibutuhkan dalam perkembangan folikel ovarium sehingga berpengaruh pada berat ovarium. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak etanol biji terhadap jumlah folikel dan berat ovarium tikus putih betina galur wistar. Studi eksperimental menggunakan randomised post test only control group design dilakukan terhadap hewan coba tikus putih betina (Rattus norvegicus) galur Wistar. Pembagian kelompok meliputi kontrol (K) (0 mg/g BB), perlakuan 1 (P1) (0,1 mg/g BB), perlakuan 2 (P2) (0,2 mg/g BB), dan perlakuan 3 (P3) (0,3 mg/g BB). Parameter dalam penelitian ini meliputi jumlah folikel (folikel preantral dan antral) dan berat ovarium. Hasil penelitian menunjukkan bahwa kenaikan jumlah dosis ekstrak etanol biji pepaya yang diberikan tidak berbeda secara signifikan pada penurunan jumlah folikel preantral (p-value=0,974), namun berpengaruh secara signifikan pada penurunan jumlah folikel antral (p-value=0,044). Terbukti adanya perbedaan rerata berat ovarium yang signifikan pada kenaikan dosis ekstrak etanol biji pepaya yang diberikan kepada tikus putih betina (p-value=0,003). Hasil uji korelasi Rank Spearman yang bermakna hanya ditunjukkan dari hubungan dosis perlakuan dan jumlah folikel antral (p-value=0,016). Kesimpulan: pemberian ekstrak etanol biji pepaya terbukti dapat mempengaruhi penurunan jumlah folikel antral, namun tidak terbukti menurunkan jumlah folikel preantral dan berat ovarium tikus putih betina galur wistar. Dosis ekstrak etanol biji pepaya yang memiliki pengaruh paling besar terhadap jumlah folikel antral tikus putih betina pada dosis 0,3 mg/g BB.

Effect of Phaleria macrocarpa Extract on NF-KB, MMP-2, and MMP-9 Expression in Endometriosis Mice Model Annissa Febriani; Sutrisno Sutrisno; Yahya Irwanto; Irfani Baihaqi; I Wayan Arsana Wiyasa; Bambang Rahardjo
Asian Journal of Health Research Vol. 1 No. 3 (2022): Volume 1 No 3 (December) 2022
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v1i3.35

Introduction: Endometriosis is the most common disease that affects the reproductive health and function of women of reproductive age. Treatment for endometriosis includes surgery and medication. Phaleria macrocarpa is a plant native to Indonesia that contains bioactive fractions known to have antiproliferative and apoptotic effects. Therefore, this study aimed to evaluate the effect of Phaleria macrocarpa extract on matrix metalloproteinases (MMP-2 and MMP-9) and NF-KB expression in the endometriosis mice model (Mus musculus). Methods: This study used a Randomized Post-Test Only with Control Group Design. Involves six groups, namely a negative control group (healthy mice without flavonoids from Phaleria macrocarpa fruit extract), a positive control group (an endometriosis model without being given flavonoids from Phaleria macrocarpa fruit extract), and a treatment group, namely a group given four different doses of flavonoids from Phaleria macrocarpa fruit extract: 3.75 milligrams per day, 7.5 milligrams per day, 11.25 milligrams per day and 15 milligrams per day. expressions of NF-KB, MMP-2, and MMP-9 were seen using Immunohistochemistry staining and observed under a microscope with 40x magnification. The data collection used an immunoratio technique with ImageJ analysis software. Furthermore, data analysis using the one-way ANOVA method. Results: In this study, the treatment group with four different doses of Phaleria macrocarpa extract could reduce the expression of MMP-2, MMP-9, and NF-KB. Groups with higher doses of Phaleria macrocarpa extract showed lessen of MMP-2, MMP-9, and NF-KB expression. Conclusion: In the endometriosis mice model, Phaleria macrocarpa fruit can reduce NF-KB, MMP-2, and MMP-9 expression.

The Effect of Flavonoid Extract from Phaleria macrocarpa to Proliferating Factors (MMP-1, MMP-3, MMP-7) in Endometriosis Mice Model Irwanto, Yahya; Wiyono , Teguh; Wardani, Kusuma
Asian Journal of Health Research Vol. 2 No. 3 (2023): Volume 2 No 3 (December) 2023
Publisher : Ikatan Dokter Indonesia Wilayah Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55561/ajhr.v2i3.102

Introduction: Endometriosis is an inflammatory disease influenced by estrogen characterized by ectopic growth of the endometrial stroma and glands. Matrix metalloproteinase (MMP) is a proteolytic enzyme that has an important role in the remodeling of the extracellular matrix in normal tissues but also contributes to pathologies such as tumor invasion. The therapy has many drawbacks, including being expensive and in need of herbal therapy as an alternative. Phaleria macrocarpa is a native plant of Indonesia that contains flavonoids. This study aimed to evaluate the effect of Phaleria macrocarpa flavonoid isolate on the development of apoptosis, proliferation, and angiogenesis in mice model endometriosis. Material and Methods: This research is a true experimental study with a Randomized Post-Test Only with a Control Group in the laboratory. Samples are divided into six groups, a control group and an intervention group, administered with flavonoid extract from Phaleria macrocarpa. Data analysis was carried out by using the Independent T-Test with SPSS for Windows 19.0 software. Results: Each control and intervention group consisted of 6 mice. The normality test for each variable shows p> 0.05. Administration of flavonoid in each group shows a significant decrease in MMP-1 (20.4 ± 7.74 vs 65.68 ± 10.97, p=0.000), MMP-3 (53.34 ± 9.66 vs67.47 ± 10.05, p=0.000), and MMP-7 expression (40.52 ± 5.43 vs 54.13 ± 4.08, p=0.000). Conclusion: Flavonoids from the Phaleria macrocarpa fruit extract were able to reduce the expression of MMP-1, MMP-3, and MMP-7 in mice model endometriosis.

Title

Found 19 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 19 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 19 Documents
Search