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Induksi Keragaman Somaklonal Bunga Kertas (Zinnia sp.) Sebagai Upaya Pengembangan Bunga Potong Daerah Tropis Kuswandi, Paramita Cahyaningrum; Sugiyarto, Lili
Jurnal Sains Dasar Vol 2, No 1 (2013): April 2013
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4380.432 KB) | DOI: 10.21831/jsd.v2i1.3578

Abstract

Abstrak Keragaman bunga kertas saat ini berasal dari persilangan atau mutasi yang dilakukan oleh manusia. Penelitian ini bertujuan untuk menginduksi keragaman bunga kertas dengan metode kultur jaringan. Penelitian dilakukan di Laboratorium Kultur Jaringan dan Kebun Percobaan FMIPA, UNY. Metode yang digunakan dalam penelitian ini adalah induksi menggunakan sitokinin BAP (benzyl amino purin) dalam media MS. Eksplan diperoleh dari tanaman bunga kertas yang sudah dikecambahkan di tray perkecambahan dan penanaman  in vitro. Sterilisasi untuk penanaman dengan teknik kultur jaringan menggunakan detergen, alkohol 70%, Clorox 10% dan 5%,  serta aquadest steril. Media yang digunakan adalah media MS (Murashige dan Skoog) ditambah dengan BAP (konsentrasi 2,4,6, dan 15ppm). Hasil penelitian menunjukkan penambahan BAP dalam media MS mampu memicu pertumbuhan kalus pada eksplan nodia dan daun Zinnia sp. Konsentrasi 4 dan 6 ppm BAP mampu menginduksi tunas dari kalus pada eksplan nodia dan keragaman hanya dapat dilihat pada tipe kalus. Terdapat perbedaan tipe kalus jika konsentrasi BAP sangat tinggi (15 ppm). Pengamatan pada sel-sel ujung akar hanya menunjukkan sel-sel berukuran kecil dengan inti sel tanpa terlihat kromosom di dalamnya. Perlu penelitian lebih lanjut untuk induksi tunas atau embrio somatik dari kalus hasil induksi BAP pada media. Pengamatan kromosom dapat diperbaiki dengan penambahan zat kimia lain atau perubahan waktu pengambilan sampel dan perendaman. Kata Kunci : keragaman, Zinnia, BAP, kromosom
APPLICATON OF MYCORRIZA ON PLANTING MEDIA OF TWO TOMATO VARIETIES TO INCREASEVEGETABLE PRODUCTIVITY IN DROUGHT CONDITION Kuswandi, Paramita Cahyaningrum; Sugiyarto, Lili
Jurnal Sains Dasar Vol 4, No 1 (2015): April 2015
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.868 KB) | DOI: 10.21831/jsd.v4i1.8432

Abstract

A method used for the development of dry areas/marginal lands is the improvement of soil structure and addition to the media to support the growth and development of crops. Tomatoes have the potential to be developed in marginal lands due to its high nutrition, high in demand and easy to be cultivated. One of the method used to improve planting media is the addition of microorganism such as mycorrhiza which can help the absorbtion of water and nutrition for plants. The interval of irrigation is used as a simulation of drought. This research aim was to observe the effect of mycorrhiza in the soil on the growth and development of tomato with several treatments of irrigation. The method used was the addition of 4 g of mycorrhiza  per polybag (size 30x30 cm2), using Complete Randomized Design. There were 6 combinations of treatments. The treatments were : 3 interval of irrigation (every day, every 7 days and every 14 days), and 2 treatments of mycorrhiza (0 g and 4 g). There were 3 repetition for each combination of treatments. The results showed that the addition of mycorrhiza can increase significantly plant fresh and dry weight and also root length. The difference in plant height, number of branches, number of leaves, plant growth rate and percentage of infection were caused by the difference in irrigation interval. The difference in the varieties used also contribute to a difference in the percentage of infection. Further research must be made on the effect of mycorrhiza with addition of inorganic fertilizer to increase the growth and development of tomato plants in water stressed condition. Keywords:   mycorriza, tomato, draught simulation
KEANEKARAGAMAN ANGGREK ALAM DAN KEBERADAAN MIKORIZA ANGGREK DI DUSUN TURGO PAKEM, SLEMAN YOGYAKARTA Sugiyarto, Lili; Umniyatie, Siti; Henuhili, Victoria
Jurnal Sains Dasar Vol 5, No 2 (2016): October 2016
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1069.878 KB) | DOI: 10.21831/jsd.v5i2.13715

Abstract

Penelitian ini bertujuan untuk melihat keanekaragaman, mikoriza simbion, dan keberadaan mikoriza di akar anggrek alam di Dusun Turgo Pakem Yogyakarta. Metode yang digunakan dengan mengidentifikasi jenis anggrek, mengisolasi dan mengkarakterisasi fungi mikoriza adalah media PDA (potato dextrose agar) yang dilanjutkan dengan identifikasi setiap fungi yang diperoleh. Pengukuran faktor lingkungan abiotik meliputi faktor edafik yaitu kelembaban, pH, suhu tanah, dan mikroklimat yaitu suhu udara, kelembaban udara, dan intensitas cahaya. Infeksi mikoriza diamati dengan mengamati keberadaan fungi pada jaringan akar. Hasil pengamatan menunjukkan adanya 8 spesies anggrek alam yaitu : Zeuxine petakinensis, endrobium sagittatum, Bulbophyllum chaetonium, Dendrobium mutabile, Eria retusa, Eria oblitterata, Pholidota carnea, Rhomboda velutina. Beberapa anggrek spesies di hutan tersebut dalam keadaan dorman sehingga tidak dapat diidentifikasi.  Keberadaan mikoriza dari hasil pengamatan mikroskopis menunjukkan bahwa pada umumnya akar anggrek spesies yang ditemui berasosiasi simbiose dengan mikoriza yang masing-masing menunjukkan jenis fungi yang berbeda. Fungi yang dapat didentifikasi diantaranya termasuk dalam genus Rhizoctonia, Glomus, Chaetonium, Scopulariopsis dan TrichodermaKata kunci: spesies anggrek, mikoriza, fungi anggrek Abstract This research aims to identify the diversity of orchids and to find the existence of mycorrrhizal symbiont in wild orchid roots in Turgo village, Pakem Sleman.                 The samples of orchids and orchid roots were done by taking samples in Puspa Forest of Turgo Village, Pakem Sleman Yogyakarta. This research were exploration research on existing orchids’ diversity. The methods that have been used to identify the existing orchid species in the forest, isolation, and characterization mycorrhizal fungi is PDA (potato dextrose agar). Measurement of environmental factors for including edhapic factors were humidity, pH, soil temperature, and microclimate factors were air temperature, air humidity, and light intensity. The mycorrhizal infection was done by observing of mycorrhizal fungi existence.            The results of the research are retrieved 8 species of orchids are Zeuxine petakinensis, Dendrobium sagittatum, Bulbophyllum chaetonium, Dendrobium mutabile, Eria retusa, Eria oblitterata, Pholidota carnea, Rhomboda velutina. Some orchids in the forest are dormant, therefore it couldn’t be identified. The mycorrhizal symbiosis from microscopic characterization had been known as different on each mychorriza. The mycorrhizal fungi that have been identified were genus of Rhizoctonia, Glomus, Chaetonium, Scopulariopsis and Trichoderma. Keywords: orchid species, mychorrhiza, orchid fungi
EFFECT OF 2,4-DICLOROFENOKSIASETAT (2,4-D) AND BENZYL AMINOPURIN (BAP) ON THE GROWTH OF BINAHONG LEAF CENTER (ANREDERA CORDIFOLIA L.) AND ANALYSIS OF FLAVONOID TOTAL CONTENT Lili Sugiyarto; Paramita Cahyaningrum Kuswandi
Jurnal Penelitian Saintek Vol 19, No 1: April 2014
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1164.539 KB) | DOI: 10.21831/jps.v19i1.2322

Abstract

The aim of this research was to study the effect of  various concentration of Plant Growth Regulator in MS (Murashige and Skoog) media on callus growth of binahong leaf and total flavonoid content. The method used in the propagation of callus was the leaf explant of binahong with a Completely Randomized Design (CRD). The leaf explants were planted on MS media with different 2,4-D concentrations (1;2;3 ppm), 0,5ppm IBA+0,5ppm BAP; 0,5ppm IBA+1,0 ppm BAP ; 1,0 ppm IBA+0,5 ppm BAP, each with 15 repetition. The parameters observed in this research were initiation time, type, colour, diameter, the number of callus and total flavonoid content.  The result showed that the optimum growth of callus is at 3 weeks and after  that it declined or stayed stagnant. The result of the analysis of variance (ANOVA) showed that there is no significant difference in the media used in this research. The total flavonoid content of fresh leaf sample  is higher than callus sample. 
OPTIMASI MEDIA KULTUR IN VITRO ANGGREK Dendrobium nobile BERBASIS PUPUK DENGAN PENAMBAHAN AIR KELAPA DAN VITAMIN B1 Ayu Purnamasari; Ratnawati Ratnawati; Aloysius Suyitno; Lili Sugiyarto; Ixora Sartika Mercuriani
Jurnal Penelitian Saintek Vol 25, No 2 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v25i2.34267

Abstract

Tujuan penelitian ini adalah mengetahui pengaruh kombinasi penambahan air kelapa dan vitamin B1 terhadap pertumbuhan anggrek Dendrobium nobile serta mengetahui konsentrasi vitamin B1 yang optimum untuk pertumbuhan anggrek pada media kultur in vitro berbasis pupuk. Penelitian ini merupakan penelitian eksperimen dengan menggunakan rancangan tersarang (nested design) dengan faktor I adalah perlakuan air kelapa (dengan air kelapa dan tanpa air kelapa) dan faktor II adalah variasi konsentrasi vitamin B1 (0 ml.L-1, 0,25 ml.L-1,0,5 ml.L-1, dan 1 ml.L-1). Sampel yang digunakan adalah bibit anggrek D. nobile umur 11 bulan dalam kultur in vitro yang mempunyai keseragaman karakter pertumbuhan. Data penelitian ini dianalisis menggunakan analisis ragam (Anova) metode rancangan tersarang dan uji Least Significant Difference (LSD). Hasil penelitian menunjukkan bahwa penambahan air kelapa memberikan pengaruh yang signifikan terhadap pertambahan diameter batang dan berat basah tanaman sedangkan penambahan vitamin B1 memberikan pengaruh yang signifikan terhadap pertambahan jumlah daun, jumlah akar, dan panjang akar anggrek. Konsentrasi vitamin B1 yang optimum untuk pertumbuhan anggrek adalah 0,5 ml.L-1 baik pada media yang mengandung ataupun tidak mengandung air kelapa.OPTIMATION FERTILIZER BASED IN VITRO CULTURE MEDIA COCONUT WATER AND B1 VITAMIN ADDITION FOR Dendrobium nobileThis study was aimed at determining the effect of the coconut water and vitamin B1 as a combination on the growth of Dendrobium nobile orchids and determining the optimum concentration of vitamin B1 for orchid growth in fertilizer-based in vitro culture media. This study was an experimental study using a nested design which factor I was the treatment of coconut water (with and without coconut water) and factor II was the variation in the concentration of vitamin B1 (0 ml.L-1, 0.25 ml. L-1, 0.5 ml.L-1, and 1 ml.L-1). The samples used were 11 months old D. nobile orchid seeds in in vitro culture which had simultaneous growth characters. The data were analyzed using Anova, nested design method and Least Significant Difference (LSD) test. The results show that the addition of coconut water has a significant effect on the increase in stem diameter and plant wet weight. The addition of vitamin B1 has a significant effect on the increase in the number of leaves, the number of roots, and the length of the orchids. The optimum concentration of vitamin B1 for orchid growth is 0.5 ml.L-1 both in media with or without coconut water.
Callus induction of binahong leaves (Anredera cordifolia L.) for the development of traditional medicinal plant Lili Sugiyarto; Paramita Cahyaningrum Kuswandi
Jurnal Sains Dasar Vol 3, No 1 (2014): April 2014
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.665 KB) | DOI: 10.21831/jsd.v3i1.2787

Abstract

A plant that has a good prospect in the development and cultivation as medicinal plant is binahong (Anredera cordifolia L.). Secondary metabolites have been used as coloring substance, food coloring, and medicine. Callus induction using tissue culture has been used to produce secondary metabolite with a higher concentration than obtained directly from the plant. The aim of this research was to induce callus from the leaf of Binahong using various concentrations of Plant Growth Hormones in MS media. The method used in the propagation of callus used the leaf explant of binahong with Completely Randomized Design (CRD). The leaf explants were planted on MS media with different 2,4-D concentrations (1;2;3 ppm), 0,5ppm IBA+0,5ppm BAP; 0,5ppm IBA+1,0 ppm BAP; and 1,0 ppm IBA+0,5 ppm BAP, each with 15 repetitions. The result showed that the addition of 2,4-D (1 and 2 ppm) was able to induce callus from Binahong leaves on 3 and 5 days after planting, and also 10 days after planting in the controlled and combination treatment. In the 2,4-D media (1 and 2 pm), the callus produced were watery white and compact, while in the 3 ppm 2,4-D media the callus were white and friable. In the media with a combination of BAP and INA the callus were green and compact. The highest average diameter of the callus after 8 weeks in the 1 ppm 2,4-D media reached 2.07 cm followed by the 2 ppm and 3 ppm 2,4-D media producing a diameter of 1.8 cm. In the combination media (BAP and IBA), the callus diameter was less than 0.3 cm. The highest percentage of callus formed was in the media MS+1 ppm 2,4-D and a combination of MS +0,5ppm IBA+0,5ppm BAP that reached 100%. The lowest was found in the control (MS) which reached only 20%. The optimum growth of callus was at 3 weeks and after  that it declined or stayed stagnant. The result of the analysis of variance (ANOVA) showed that there was no significant difference in the media used in this research. Key words: induction, binahong leaf, callus, plant growth
Eksplorasi Metode Sterilisasi dan Macam Media Untuk Perbanyakan Durian (Durio zibethinus, L.) Secara In Vitro Lili Sugiyarto; Paramita Cahyaningrum Kuswandi
Jurnal Sains Dasar Vol 2, No 1 (2013): April 2013
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.801 KB) | DOI: 10.21831/jsd.v2i1.2374

Abstract

Abstrak Durian (Durio zibethinus, L.) memiliki nilai ekonomis tinggi baik sebagai salah satu komoditas hortikultura yaitu buah untuk konsumsi di pasar lokal maupun untuk ekspor. Salah satu hambatan adalah persediaan bibit yang tidak mencukupi dan metode perbanyakan yang merusak pohon induk dengan karakter unggul. Penelitian ini bertujuan untuk memperoleh metode sterilisasi yang tepat dan media untuk perbanyakan durian secara in vitro. Penelitian dilakukan di Laboratorium Kultur Jaringan dan Kebun Percobaan FMIPA, UNY. Metode yang digunakan dalam penelitian ini adalah induksi menggunakan sitokinin BAP (benzyl amino purin) dalam media MS. Eksplan diperoleh dari bibit durian yang sudah dikecambahkan di polibag dengan media tanah dan kompos. Sterilisasi untuk penanaman dengan teknik kultur jaringan menggunakan detergen, clorox 20% dan 10%, alkohol 70%, dan aquades steril untuk nodia, sedangkan sterilisasi eksplan daun dengan clorox 10% dan 5%. Media yang digunakan adalah media MS (Murashige dan Skoog) ditambah dengan BAP (konsentrasi 2 dan 4 ppm), serta 2,4-D (0.4, 1.0, dan 1.5 ppm). Hasil penelitian menunjukkan penambahan BAP dalam media MS mampu memicu pertumbuhan tunas pada eksplan nodia durian pada konsentrasi 2 dan 4 ppm. Penambahan 2,4-D mampu menginduksi kalus pada daun durian. Pada konsentrasi 1 ppm kalus yang dihasilkan berwarna putih, berair dengan daun yang tidak terlalu menggulung sedangkan konsentrasi 0,4 dan 1,5 ppm menghasilkan kalus hijau dengan daun yang menggulung. Perlu perlakuan media yang lebih bervariasi baik pada eksplan nodia maupun daun untuk menghasilkan planlet melalui kalus atau pembentukan tunas secara langsung.   Kata kunci: durian, sterilisasi, BAP, 2,4-D, tunas, kalus   Abstract One of the problems that can occur in the development of durian (Durio zibethinus, L.)  is the availability of seedlings and the method of propagation can cause over-exploitation of the mother plant. The aim of this research was to obtain the method of sterilization and the right media for in vitro propagation of durian. The research was carried out in the Tissue Culture Laboratory and Experimental Garden Faculty of Mathematics and Science, Yogyakarta State University. The method used in this research was induction using the cytokinin BAP (benzylamino purine) in MS media. The explants were obtained from durian seedlings that were grown in polybags with soil and compost media. Sterilization for the tissue culture technique was done using detergent, alcohol 70%, and clorox (20% and 10%), and also sterile aquadest for nodia, while leaf exsplant sterilization was done using clorox 10% and 5%. The media used were MS (Murashige and Skoog) with BAP (2 dan 4 ppm) for the nodia and also with 2,4-D (0.4, 1.0, dan 1.5 ppm) for the leaf explants. The result showed that the BAP in the MS media was able to induce the growth of shoots from the nodia at concentrations of 2 and 4 ppm. The addition of 2,4-D was able to induce the growth of callus on the leaf explants. Using 1 ppm 2,4-D, the callus was white and transparent and the leaves did not curl so much as in the treatments with 0.4 and 1.5 ppm 2,4-D which caused the leaves to curl. More variation in the treatments or media both for the nodia and leaves explants can be further investigated to produce plantlets via callus induction or direct organogenesis. Key words: durian, sterilization, BAP, 2,4-D,  shoot, callus
PENGARUH VARIASI TANAMAN BARIER SEBAGAI NATURAL PEST CONTROL TERHADAP POPULASI HAMA DAN PRODUKTIVITAS PADI Tien Aminatun; Budiwati Budiwati; Lili Sugiyarto; Amin Khusnadiyah; Anisa Setya Hidayah; Ema Imtihana
Jurnal Penelitian Saintek Vol 25, No 1 (2020)
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.946 KB) | DOI: 10.21831/jps.v25i1.29999

Abstract

Tujuan dari penelitian ini adalah untuk menganalisis pengaruh variasi jenis tanaman barier terhadap dinamika populasi serangga hama pada tanaman padi dan produktivitas tanaman padi. Penelitian ini dilakukan di Kebun Percobaan Fakultas Pertanian UGM Banguntapan Bantul. Variabel bebas dari penelitian ini adalah perlakuan variasi tanaman barier yaitu kontrol (tanpa barier), gulma alami, bunga matahari, dan bunga kenikir. Variabel terikatnya adalah dinamika populasi serangga hama dan produktivitas tanaman padi. Pengamatan serangga hama dan musuh alaminya dilakukan terbatas pada pagi hari antara pukul 08.00-11.00 dengan metode scan sampling yang dilakukan pada 3 minggu sekali. Data produktivitas tanaman padi yang diambil meliputi berat kering gabah hasil panen per plot perlakuan. Untuk melihat pengaruh perlakuan variasi jenis tanaman barier terhadap populasi serangga hama tanaman padi dan produktivitas tanaman padi dilakukan uji ANOVA. Hasil penelitian menunjukkan tidak terdapat perbedaan yang signifikan dari pengaruh variasi jenis tanaman barier terhadap perbedaan dinamika populasi serangga hama maupun terhadap produktivitas tanaman padi, tetapi perlu diadakan penelitian lanjutan yang dikaitkan dengan variabel lain yang mempengaruhi produktivitas tanaman padi.THE EFFECT OF BARRIER PLANT VARIATION AS NATURAL PEST CONTROL ON PEST POPULATION AND RICE PRODUCTIONThis study was aimed at analyzing the effect of the barrier plant variation to the population dynamic of rice pest insect and on rice crop productivity. The study was conducted in the experimental Garden of Faculty of Agriculture of Gadjah Mada University in Banguntapan Bantul. The independent variable was the treatment of barrier plant variation, i.e. control (without barrier), natural weed, sunflower and kenikir flower plants, while the dependent variables were population dynamic of pest insect and rice crop productivity. Pest insects and their natural enemies observation was carried out in between 08.00-11.00 am by scan sampling every 3 weeks. The retrieval of crop productivity data was done when the harvest per treatment plot. The data obtained was then tested with ANOVA, to find out the effect of the treatments on population dynamic of rice crop pest, as well as rice crop productivity. The results show that there was no significant difference in the effect of barrier plant variation to the differences in population dynamic of pest insects; as well as in the productivity of rice crop, but it is necessary to conduct advanced research that is associated with other variables that affect rice crop productivity.
PENGENALAN KEANEKARAGAMAN JENIS ANGGREK DAN POTENSI BISNISNYA Ixora Sartika Mercuriani; Lili Sugiyarto
Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA Vol 3, No 2 (2019): Vol 3, No 2 (2019)
Publisher : Yogyakarta State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.23 KB) | DOI: 10.21831/jpmmp.v3i1.16720

Abstract

Program pengabdian kepada masyarakat ini bertujuan menumbuhkan semangat untuk melestarikan tanaman anggrek dengan  mengenalkan keanekaragaman jenis anggrek yang dapat digunakan sebagai wahana belajar siswa, mengenalkan teknik  persilangan anggrek, dan mengenalkan budidaya anggrek untuk alternatif bisnis keluarga. Kegiatan ini diikuti oleh guru dan karyawan SD Muhammadiyah Sleman yang berjumlah 27 orang. Metode kegiatan ini adalah pemberian materi oleh tim pengabdi tentang keanekaragaman anggrek dan metode budidayanya, perhitungan biaya bisnis secara garis besar, dilanjutkan dengan pelatihan teknik persilangan anggrek dan aklimatisasi anggrek. Hasil pengabdian menunjukkan semua peserta (100%) telah paham tentang keanekaragaman anggrek, teknik persilangan anggrek dan budidaya tanaman anggrek melalui metode aklimatisasi anggrek yang sebelum adanya pelatihan ini lebih dari 90% peserta belum pernah melakukan teknik perbanyakan anggrek tersebut. Hasil penjaringan angket kepuasan peserta adalah baik untuk semua aspek kecuali kesesuaian waktu pelaksanaan. Para peserta juga sangat antusias dalam mengikuti pelatihan. Kegiatan dilanjutkan dengan pendampingan, evaluasi dan perbaikan. Kata kunci :
PENGARUH VARIASI DOSIS KMnO4 TERHADAP KADAR GULA PEREDUKSI DAN KEBERADAAN JAMUR PADA PASCAPANEN BUAH TOMAT (Lycopersicon esculentum Mill) VARIETAS SERVO Shinta Kartika Dewi; Yuliati Yuliati; Lili Sugiyarto
Kingdom (The Journal of Biological Studies) Vol 5, No 5 (2016): Jurnal Prodi Biologi
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v5i5.5884

Abstract

Penelitian ini bertujuan untuk mengetahui dosis efektif KMnO4 yang diberikan terhadap mutu buah tomat pascapanen,  pengaruh pemberian variasi dosis KMnO4 terhadap kadar gula pereduksi yang terkandung dalam buah tomat varietas Servo, pengaruh pemberian variasi dosis KMnO4 terhadap keberadaan mikroba pada buah tomat varietas Servo dan pengaruh pemberian variasi dosis KMnO4 terhadap warna buah tomat varietas ServoJenis Penelitian berupa penelitian observasi dengan mengetahui pengaruh beberapa dosis KMnO4 terhadap kadar gula pereduksi, keberadaan jamur dan warna buah pada tomat pascapanen. Populasi Penelitian adalah buah tomat varietas Servo dengan sampel 180 buah tomat yang sudah matang dan berat seragam yaitu 20-70 gram. Pengambilan sampel menggunakan teknik purposive sampling. Data hasil pengamatan dianalisis dengan menggunakan analisis univariate dengan uji Duncan’s Multiple Range Test (DMRT).Hasil penelitian menunjukkan bahwa dosis KMnO4 yangefektif dalam menghambat pematangan buah yaitu konsentrasi 135 ppm. Dosis ini memberikan pengaruh terhadap buah tomat dengan adanya perlambatan tertinggi dalam proses perubahan kadar gula pereduksi dan warna buah. Parameter keberadaan jamur tidak menunjukkan respon terhadap pemberian variasi dosis KMnO4 . Kata kunci: Tomat varietas Servo, Kalium Permanganat (KMnO4), gula  pereduksi, jamur