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Journal : Jurnal Pembelajaran dan Biologi Nukleus

Analysis of Amylase Activity in Bacteria Isolated from Hot Spring of Pentadio Resort Nur'ain Marton Angio; Aryati Abdul; Syam S. Kumaji; Wirnangsi Din Uno; Yuliana Retnowati; Magfirahtul Jannah
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS (JPBN) Vol 10, No 1 (2024): Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5506

Abstract

The hot spring of Pentadio Resort is one of the geothermal areas likely to be the place of bacteria or microorganisms that can produce amylolytic enzymes. This research aims to analyze bacteria in the Pentadio Resort hot springs that possess the potential to produce amylase enzymes, analyze the characteristics of bacterial isolates that produce amylase enzymes in Pentadio Resort hot springs and determine the types of bacteria that produce amylase enzymes in Pentadio Resort hot springs. The research employs a qualitative and descriptive method. The research involved several methods, including sample collection, media preparation, isolation and selection of thermophilic bacterial isolates, purification of thermophilic bacterial isolates, selection of isolates producing amylase enzymes, identification of selected isolates (motility test, biochemical test, gram staining), and molecular identification. The research yielded seven isolates, and isolate code A6 demonstrated the potential to produce amylase enzymes with a clear zone size of 23,26 mm, bacillus, gram positive, and positive motility. Subsequently, molecular identifidation was carried out in which, based on the results of phylogenetic tree reconstractiopisalate A6 was related to the bacteria Bacillus cereus strain MD 152, which belongs to the Bacillus cereus bacteria
Development of Interactive E-Books Oriented Towards Science Literacy Skills on Immune System Material Kartika, Dika; Uno, Wirnangsi Din; Dama, Lilan; Ibrahim, Mustamin; Solang, Margaretha; Hasan, Ani M.
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4965

Abstract

This research aims to get interactive e-book learning media  oriented to science literacy skills on immune system material. The e-book learning criteria  produced in this study have been valid, practical, and effective. This type of research is development research that uses 4D models (Define, Design, Develop, Disseminate). This study used 6 instruments, namely: interview instruments, validation instruments, student response instruments, learning implementation assessment instruments, student activity assessment instruments, and learning outcome assessment instruments (pre-test and post-test). The results found that the validity test based on the assessment results of validators of material experts, media experts, question experts and teachers obtained x ̅ scores of 98%, 97%, 93.33%  and 91.33% (very good) respectively. The practicality test for the results of learning implementation in this study was the first meeting and the second meeting got a score of x ̅ 97%, the results of student activities in the first and second meetings got a score of x ̅  respectively 87%, and 90%, and the results of student responses in this study got a  score x ̅ is 86% (all of those results belong to the "very practical" category). The effectiveness test based on the results of N-Gain analysis in this study received a value of 0.80 (effective). The author concludes  that the interactive e-book developed is valid, practical, and effectively used in the Biology learning process at SMA Negeri 2 Gorontalo