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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) International Journal of Biosciences and Biotechnology Itepa : Jurnal Ilmu dan Teknologi Pangan Jurnal Farmasi Udayana INDONESIAN JOURNAL OF BIOMEDICAL SCIENCES The Journal of Pure and Applied Chemistry Research Indonesian Journal of Chemistry Journal of Health Sciences and Medicine
Ketut Ratnayani
Program Studi Kimia, Fakultas MIPA Universitas Udayana, Bali-Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Electrical & Electronics Engineering Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : The Journal of Pure and Applied Chemistry Research

 1 
Effect of Solvent Type and Germination Time on The Level of Free Amino Acid and Peptides of Germinated Pigeon Pea (Cajanus cajan (L.) Millsp) Extract Ketut Ratnayani; Ni Wayan Wisaniyasa; Ni Putu Wiwik Oktayuni
The Journal of Pure and Applied Chemistry Research Vol 11, No 2 (2022): Edition May-August 2022
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2022.011.02.672

Abstract

Germination can activate the degradation of storage protein in legumes releasing peptides and free amino acids for seed growth. These compounds have many benefits in many fields, especially in food and health. This study aimed to determine the effect of solvent type and germination time on the level of free amino acids (FAA) and the level of peptides (MW < 10 kDa) of germinated pigeon pea extract. The extraction of free amino acids and the dissolved protein from germinated pigeon pea flour was carried out using two kinds of solvents, namely water, and 0.1 N HCl. The variation of germination times of pigeon pea seeds was carried out at 12, 24, 36, 48, 60, 72, 84, and 96 hours. The level of FAA was determined spectrophotometrically using the ninhydrin method after the deproteination step, while the level of peptides was determined spectrophotometrically using the biuret method. The results showed that using 0.1 N HCl as a solvent produced a higher level of FAA and peptides in the extract than using water. The increase of germination time can increase the level of FAA with the peak FAA level achieved on 36 hours. The increase of germination time can increase the level of peptides with the peak of peptides level achieved on 84 hours of germination times. These results showed that seed germination is a potential method for producing free amino acids and peptides which accumulate at a specific time.
Co-Authors A. A. Ayu Septri Juwarni A. W. Dwiputri Acintya, N. M. T. C. Amanda Awalia Ramadhani Ana Listya Anak Agung Istri Agung Mayun Laksmiwati Ariati, N. K. Darma Asih Yuliana Deniariasih, N.W. Dewi, I G. A. K. S. P. Dita Rizkiyanti Helen Helda Prastika I G. A. M. A. S. Gitadewi I Gusti Ayu Kunti Sri Panca Dewi I Ketut Suter I Made Oka Adi Parwata I Nengah Kencana Putra I Nengah Wirajana I Wayan Sudiarta I Wayan Suirta I. A. Preharsini Kusuma I. A. R. Astiti Asih Ida Bagus Swardana Indriani Wisnu Susanto Panjaitan James Sibarani Lia Kusumaningrum Liangky Syane S Listiyanti, N. K. L. M. A. Pratiwi M. Nazib Made Arsa Maman Sudiarto Ni G. A. M. Dwi Adhi Suastuti Ni Komang Ariati Ni Luh Putu Mega Wahyuni Ni Made Puspawati Ni Made Suaniti Ni P. Indah Septian P. Ni Putu Frida Oktaningtias Widiarthi Ni Putu Wiwik Oktayuni Ni Wayan Agustini Ni Wayan Wisaniyasa NYOMAN SEMADI ANTARA Putra, A. A. B. Putu Eka Purnama Putu Lita Astriani Putu Setya Sri Antary Ro’yal Aini Sagun Chandra Yowani Suarsa, I W. Syamsul Arifin Tutut Hardikawati