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All Journal Jurnal Kimia (Journal of Chemistry) METAMORFOSA Journal of Biological Sciences Jurnal Veteriner Jurnal Biologi Udayana CHEMISTRY PROGRESS SIMBIOSIS JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Advances in Tropical Biodiversity and Environmental Sciences

Dwi Ariani Yulihastuti

Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana, Kampus Bukit Jimbaran, Badung, Bali

Author-ID : 2925880

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Veterinary

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PENENTUAN JENIS DAN KADAR ZAT PEWARNA MERAH PADA MAKANAN YANG BEREDAR DI SEKOLAH DASAR DI KELURAHAN JIMBARAN, KECAMATAN KUTA SELATAN, KABUPATEN BADUNG Ni Komang Lisna Putri; Ni Luh Suriani; Dwi Ariani Yulihastuti
Jurnal Biologi Udayana Vol 16 No 2 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.689 KB)

Penelitian bertujuan untuk mengetahui jenis zat pewarna merah yang dominan dan kadar zat pewarna merah pada makanan yang beredar di Sekolah Dasar di Jimbaran. Penelitian dilakukan dari bulan Agustus-September 2012. Total jumlah sampel yang diambil adalah 16 sampel makanan yang berasal dari 8 sekolah yang mana 2 sampel diambil dari masing-masing makanan. Analisa dilakukan dengan 2 metode uji yaitu uji kualitatif untuk menentukan jenis pewarna dengan kertas kromatografi dan uji kuantitatif untuk menentukan kadar zat pewarna sintetis menggunakan metode spektrofotometri. Hasil penelitian menunjukkan bahwa terdapat 3 jenis zat pewarna makanan yang digunakan yaitu Rhodamin B, Allura Red dan Ponceau 4R. Kadar rata-rata pewarna tertinggi terdapat pada Ponceau 4R (10,046 ppm) dan terendah terdapat pada Allura Red (6,4456 ppm). Konsentrasi tersebut masih berada dibawah batas maksimum penggunaan pewarna makanan yang ditetapkan oleh pemerintah yaitu 70-300 ppm.

HUBUNGAN ANTROPOMETRI TULANG PANJANG TERHADAP TINGGI BADAN MAHASISWA SUKU BATAK TOBA DI KOTA BEKASI Ezra Oktavia Ambarita; Iriani Setyawati; Dwi Ariani Yulihastuti
SIMBIOSIS Vol 10 No 1 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Forensic anthropology is the application of a specific branch of biological anthropology based on human bones and anatomy. Anthropometry is a method of measuring body parts. Height measurement is a forensic anthropological parameter that helps build a person's biological profile. Height can be determined by the size of the long bones including the humerus, radius, ulna, femur, tibia, and fibula. This research on height was carried out on Batak Toba students. This study aims to determine the anthropometric relationship of long bone length to the height of Batak Toba students in the city of Bekasi. This research is an analytic observational study with a cross sectional approach. This research was conducted in the city of Bekasi with the research subjects totaling 60 students consisting of 30 men and 30 women with an age range of 18-22 years from the Toba Batak tribe who did not have bone disorders. Samples were taken by purposive sampling technique. Data analysis was performed by Pearson Correlation (r) test. The parameters analyzed in this study were length of humerus, radius, ulna, femur, tibia, fibula, height, sitting height, weight, and body mass index. The results of the Pearson Correlation test analysis (r) showed that there is a significant relationship between the length of the humerus, radius, ulna, femur, tibia, fibula and sitting height with a strong to perfect correlation, while the relationship between body mass index and height there is no significant difference and there is no correlation to Batak Toba students in the city of Bekasi. Keywords: height, long bones, anthropometry, Batak Toba

STRUKTUR HISTOLOGI TRAKEA TIKUS PUTIH (Rattus sp.) YANG TERPAPAR ASAP ROKOK SETELAH DIBERI EKSTRAK BUAH JUWET (Syzygium cumini L.) I Komang Angga Kristiawan; Ni Made Rai Suarni; Dwi Ariani Yulihastuti
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

This study aimed to determine the effect of juwet fruit extract on histological structure of rat(Rattus sp) trachea which exposed to cigarette smoke. This research used Completely Randomized Design (CRD), with four treatments: the control group (K0) treated with 0.5 % CMC–Na, (K1) group is exposed to cigarette smoke, (K2) group were given juwetfruit extract, and (K3) group is exposed to cigarette smoke and juwet fruit extracts. Each treatment consisted of 6 rats as replication. The exposure to cigarette smoke is given from an aerator pump lit cigarettes. Juwet fruit extract and 0.5 % CMC - Na was orally administered (gavage method) for 48 days. The existence of comperative descriptive observed cilia. And goblet number, high epithelium and lumen diameter Data were analyzed with ANOVA and If they were 5 % significantly different would be followed by Duncan test. Results showed that the extract of the fruit juwet significant effect on the histological structure of the trachea mice that had been exposed to smoke.

Labia and Lingua Histopathology of Bali Cow (Bos sondaicus) on Hepatogenous Photosensitization Symptoms in Pakutatan Village, Jembrana, Bali Kadek Mardika; Iriani Setyawati; Dwi Ariani Yulihastuti
Advances in Tropical Biodiversity and Environmental Sciences Vol 2 No 2 (2018): ATBES
Publisher : Institute for Research and Community Services Udayana University

Hepatogenous photosensitization is one of the ruminant diseases with symptoms of dermatitis or eczema of the skin accompanied by liver damage. The disease is caused by the compounds of toxic lantadene A and lantadene B which are secondary metabolites of Lantana camara plant. This research was carried out on January 2017. The material used in this study was preserved organs of the labia and lingua of dead three year old cow (Bos sondaicus). Samples were taken from Pakutatan Village, Jembrana, Bali. Identification of organ samples, histological preparation and histopathological examination were conducted at the Disease Investigation Center (DIC) 6, Denpasar, Bali. The preparation of labia and lingua samples used the paraffin method with Hematoxylin and Eosin staining. The tissue structural damages found were necrosis, inflammatory cell infiltration, congestion and tissue bleeding. The data obtained were analyzed statistically by One Way Anova test with a confidence level of 95%. Based on the results, dead Bali cow which consumed a large numbers of Lantana camara plants showed that the highest number of cell damage was at the picnotic stage of cell necrosis (cell death) in the labia organ significantly (P<0.005), while the highest bacterial invasion was found in the labia organ with an average percentage of 12.40%.

Determination of Flavonoid, Tannin and Vitamin C Content from Methanol Extract Wrapping Stone Banana (Musa brachycarpa), Ketip Banana (Musa Paradisiaca Forma Typiaca) and Kepok Banana (Musa acuminata) Ni Putu Adriani Astiti; Dwi Ariani Yulihastuti
Advances in Tropical Biodiversity and Environmental Sciences Vol 1 No 2 (2017): ATBES
Publisher : Institute for Research and Community Services Udayana University

Research has been done to investigate the levels of flavanoid, tannin, and vitamin C of methanol extract of Stone banana (Musa brachycarpa), Ketip banana (Musa paradisiaca forma typiaca ), and Kepok banana (Musa acuminata). Jukut Ares is a Balinese traditional cuisine frequently served in any events of celebrations. The use of young stems of such banana varieties as the main raw materials in the process of making jukut ares has not been scientifically elucidated. Antioxidants are compounds with ability to inhibit oxidation-related reactions due to free radicals that cause disturbance of saturated fatty acids of cell membrane, blood vessels, DNA, as well as adipose tissues which lead to occurrence of various diseases. Plants components will have antioxidant activities if they contain compounds (phenol and flavanoid) with ability to scavenge free radicals. Tannin is a water based phenolic compound and vitamin C is also a water based vitamin that play important roles in preventing us from various diseases. Extraction was conducted by applying maceration method and to investigate the levels of flavanoid, spectrophotometric UV vis with aluminum chloride (AlCl3) was applied. The level of vitamin C of the materials was conducted by Iodine titration method, while the level of tannin was determind by applying spectrophotometri. The results showed that methanol extract of Ketip banana stem contained the highest level of flavanoid with a quantity of 53.13 mg/100gr QE, and this was followed by stone banana (36.28) and kepok banana (32.07) The highest vitamin C level was indicated in the extract of ketip banana (418.32 mg/100gr), which is followed by kapok banana and stone banana. In the calculation of tannin content, pisang ketip showed the highest level, while pisang batu contained the lowest level of tannin.

Kuantitas Dan Kualitas DNA Hasil Ekstraksi Dari Bercak Darah Pada Pisau Pasca Paparan Sinar Ultraviolet Dan Matahari Putri Arie Prasetyoningrum; I Ketut Junitha; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p19

Examination of Deoxyribonucleic Acid is the primary identification method in forensic cases. Blood stains found at the scene of a crime are often affected by various environmental factors, one of which is ultraviolet light from the sun. Purpose of this study is to compare the quantity and quality of DNA extracted from blood stains on the blade after exposure to ultraviolet light and the sun for 0, 15 and 30 days. The method used is a factorial design: light source (UVA, indirect sunlight and direct sunlight); and duration of treatment (0, 15 and 30 days). Blood stains are made by dripping a 50?L of human blood on one side of the blade and then given treatment. DNA analysis includes: extraction with Chelex 5%; DNA quantity and quality test with SimpliNano spectrophotometer; total DNA quality test with agarose gel electrophoresis; and PCR. Quantitative data analysis using Univariate test followed by Duncan test. The results showed that the quantity of DNA in samples exposed to UVA and sunlight (directly) increased on the 15th day due to DNA fragmentation while samples exposed to sunlight (indirectly) decreased the quantity of DNA with the duration of treatment. DNA quality in the form of DNA purity produces an extract of DNA that is not pure. The total DNA quality with agarose gel electrophoresis on all samples showed that the longer the treatment (0, 15 and 30 days) resulted in a dimmer or thinner band of DNA luminescence with smears.

In vitro assessment of antifungal activity of cinnamon leaves extract against the Colletotrichum sp. causes of anthracnose on tomato Sudirga, Sang Ketut; Darmadi, Anak Agung Ketut; Wijaya, I Made Saka; Yulihastuti, Dwi Ariani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 2 (2024): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.224284-294

Tomato is a horticultural plant widely cultivated in Bali. Over the three years, the productivity and quality of this plant have tended to decrease due to pathogen-derived diseases, such as anthracnose caused by Colletotrichum sp. Until recently, control of this pathogenic fungus has relied on chemical-based fungicides, which lead to many long-term complications, including pathogen resistance, environmental pollution, the extinction of non-target microorganisms, and negative impacts on human health. Therefore, alternative methods for plant disease control are urgently needed to combat these pathogen attacks. The use of plant-derived active compounds has been intensively researched worldwide as a more environmentally friendly alternative. The main objective of this research was to investigate the effectiveness of Cinnamomum burmanii acetone extract in inhibiting the growth of Colletotrichum sp., the causative agent of anthracnose in tomatoes, through an in vitro approach. A non-factorial randomized complete design was applied in the experiment. The results showed that the crude extract of cinnamon leaves inhibited the growth of the Colletotrichum sp. with an MIC value of 0.9%, an inhibition zone of 2.55 mm, and an optimal inhibitory concentration of 2%, producing an inhibition zone of 11.10 mm. A GC-MS analysis was conducted to identify the active compounds in the cinnamon leaf extract. Sixteen active compounds were identified, nine of which are known to have antimicrobial activity.

Co-Authors Anak Agung Ketut Darmadi Ezra Oktavia Ambarita I Ketut Junitha I Komang Angga Kristiawan I Wayan Sudiarta IRIANI SETYAWATI Ivani Dayanara Kadek Mardika Ni Komang Lisna Putri Ni LUH ARPIWI Ni Luh Made Yuli Indra Yanti Ni Luh Suriani Ni Made Estriana Pranadya Ni Made Rai Suarni Ni Made Suartini Ni Putu Adriani Astiti Ni Putu Diantariani Ni Wayan Sudatri Putri Arie Prasetyoningrum Putu Anjarina Retno Kawuri Ruth Ellisa Christiani SANG KETUT SUDIRGA Wijaya, I Made Saka

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