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PENENTUAN JENIS DAN KADAR ZAT PEWARNA MERAH PADA MAKANAN YANG BEREDAR DI SEKOLAH DASAR DI KELURAHAN JIMBARAN, KECAMATAN KUTA SELATAN, KABUPATEN BADUNG Ni Komang Lisna Putri; Ni Luh Suriani; Dwi Ariani Yulihastuti
Jurnal Biologi Udayana Vol 16 No 2 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Penelitian bertujuan untuk mengetahui jenis zat pewarna merah yang dominan dan kadar zat pewarna merah pada makanan yang beredar di Sekolah Dasar di Jimbaran. Penelitian dilakukan dari bulan Agustus-September 2012. Total jumlah sampel yang diambil adalah 16 sampel makanan yang berasal dari 8 sekolah yang mana 2 sampel diambil dari masing-masing makanan. Analisa dilakukan dengan 2 metode uji yaitu uji kualitatif untuk menentukan jenis pewarna dengan kertas kromatografi dan uji kuantitatif untuk menentukan kadar zat pewarna sintetis menggunakan metode spektrofotometri. Hasil penelitian menunjukkan bahwa terdapat 3 jenis zat pewarna makanan yang digunakan yaitu Rhodamin B, Allura Red dan Ponceau 4R. Kadar rata-rata pewarna tertinggi terdapat pada Ponceau 4R (10,046 ppm) dan terendah terdapat pada Allura Red (6,4456 ppm). Konsentrasi tersebut masih berada dibawah batas maksimum penggunaan pewarna makanan yang ditetapkan oleh pemerintah yaitu 70-300 ppm.

Kuantitas Dan Kualitas DNA Hasil Ekstraksi Dari Bercak Darah Pada Pisau Pasca Paparan Sinar Ultraviolet Dan Matahari Putri Arie Prasetyoningrum; I Ketut Junitha; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p19

Examination of Deoxyribonucleic Acid is the primary identification method in forensic cases. Blood stains found at the scene of a crime are often affected by various environmental factors, one of which is ultraviolet light from the sun. Purpose of this study is to compare the quantity and quality of DNA extracted from blood stains on the blade after exposure to ultraviolet light and the sun for 0, 15 and 30 days. The method used is a factorial design: light source (UVA, indirect sunlight and direct sunlight); and duration of treatment (0, 15 and 30 days). Blood stains are made by dripping a 50?L of human blood on one side of the blade and then given treatment. DNA analysis includes: extraction with Chelex 5%; DNA quantity and quality test with SimpliNano spectrophotometer; total DNA quality test with agarose gel electrophoresis; and PCR. Quantitative data analysis using Univariate test followed by Duncan test. The results showed that the quantity of DNA in samples exposed to UVA and sunlight (directly) increased on the 15th day due to DNA fragmentation while samples exposed to sunlight (indirectly) decreased the quantity of DNA with the duration of treatment. DNA quality in the form of DNA purity produces an extract of DNA that is not pure. The total DNA quality with agarose gel electrophoresis on all samples showed that the longer the treatment (0, 15 and 30 days) resulted in a dimmer or thinner band of DNA luminescence with smears.

In vitro assessment of antifungal activity of cinnamon leaves extract against the Colletotrichum sp. causes of anthracnose on tomato Sudirga, Sang Ketut; Darmadi, Anak Agung Ketut; Wijaya, I Made Saka; Yulihastuti, Dwi Ariani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 2 (2024): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.224284-294

Tomato is a horticultural plant widely cultivated in Bali. Over the three years, the productivity and quality of this plant have tended to decrease due to pathogen-derived diseases, such as anthracnose caused by Colletotrichum sp. Until recently, control of this pathogenic fungus has relied on chemical-based fungicides, which lead to many long-term complications, including pathogen resistance, environmental pollution, the extinction of non-target microorganisms, and negative impacts on human health. Therefore, alternative methods for plant disease control are urgently needed to combat these pathogen attacks. The use of plant-derived active compounds has been intensively researched worldwide as a more environmentally friendly alternative. The main objective of this research was to investigate the effectiveness of Cinnamomum burmanii acetone extract in inhibiting the growth of Colletotrichum sp., the causative agent of anthracnose in tomatoes, through an in vitro approach. A non-factorial randomized complete design was applied in the experiment. The results showed that the crude extract of cinnamon leaves inhibited the growth of the Colletotrichum sp. with an MIC value of 0.9%, an inhibition zone of 2.55 mm, and an optimal inhibitory concentration of 2%, producing an inhibition zone of 11.10 mm. A GC-MS analysis was conducted to identify the active compounds in the cinnamon leaf extract. Sixteen active compounds were identified, nine of which are known to have antimicrobial activity.

KUALITAS SPERMATOZOA DAN BERAT ORGAN REPRODUKSI MENCIT (Mus musculus) SETELAH PEMBERIAN EKSTRAK BIJI KLABET (Trigonella foenum-graecum L.) Maulidya Rosa Putri Utama; Dwi Ariani Yulihastuti; Sang Ketut Sudirga
SIMBIOSIS Simbiosis Volume 12 No. 1 2024
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2024.v12.i01.p04

Indonesia is one of the countries known to have the highest biodiversity in the world. There are 18 types of herbs that have the potential as antifertility in men, one of which is the klabet plant or fenugreek. Klabet is believed by ordinary people to have properties to increase the production and quality of spermatozoa. According to previous research klabet has toxic effects that can inhibit fertility due to the content of phytochemicals in it. This study aims to determine the effect of fenugreek seed extract on the quality of spermatozoa and the weight of the reproductive organs of mice. The mice used were 20 male mice weighing about 25-30 grams divided into 4 treatments namely control (P0), 56 mg/kgBB (P1), 112 mg/kgBB (P2), 168 mg/kgBB (P3) with 5 repetitions. Giving treatment for 24 days. The method used is RAL (Complete Randomized Design) and the resulting data is analyzed using ANOVA. The parameters studied were testicular weight, epididymis weight, motility, morphology and number of male mouse spermatozoa. The results showed that there was a real difference (p≤0.05) between the control and treatment of the klabet seed extract on the quality of spermatozoa and the weight of the testicle but not significantly different (p≥0.05) to the weight of the epididyimis. Concentration of mice spermatozoa decreased by 10.60%, progressive spermatozoa motility decreased by 55.61%, nomal morphology decreased by 57.92%, while testicular weight decreased by 38.46%. The most effective dose of fenugreek seed extract used as an antifertility agent was a dose of 168 mg/kgBW (P3).

POLA PERTUMBUHAN SISWA DI SD LABORATORIUM UNDIKSHA SINGARAJA DAN SDN 3 PANJI, KABUPATEN BULELENG, BALI Ni Nyoman Dian Anjani; Ni Made Rai Suarni; Dwi Ariani Yulihastuti
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i04.

Growth is a change that occurs quantitatively that can be observed in human physique, such as height, and body weight. The purpose of this research was to determine the growth patterns of student at Elementary School (SD) LAB Undiksha Singaraja and SDN 3 Panji. The research method used purposive sampling, carried out at SD LAB Undiksha Singaraja and SDN 3 Panji with 60 probands aged 10-12 years in January 2023. Observations were made on student height, student weight, student Body Mass Index (BMI), student age at SD LAB Undiksha Singaraja and SDN 3 Panji. Data analysis used the T Test. The results of statistical analysis show that there are insignificant differences in the growth patterns of students at SDN 3 Panji and SD LAB Undiksha Singaraja. The research results showed that the average height and weight of students at SDN 3 Panji was lower than at SD LAB Undiksha Singaraja. The majority of students' BMI at SDN 3 Panji is in the underweight category, namely 60% for boys and 66.67% for girls. The majority of students' BMI at SD LAB Undiksha Singaraja is in the normal category, namely 46.67% for boys and 53.33% for girls. Keywords: Body Mass Index, Panji, growth, Undiksha

Deteksi Cemaran Total Bakteri, Coliform, Escherichia coli, dan Salmonella sp. pada Daging Ayam Broiler (Gallus domesticus) yang Dijual di Beberapa Pasar Daerah Kabupaten Badung, Bali Kadek Aryndha Sukma Prabaswari; Retno Kawuri; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 2 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i02.p4

Chicken meat is a source of animal protein which is easily damaged due to its high water content. Chicken meat that is bought and sold could potentially become a place for the growth of bacteria that cause foodborne diseases.This study aims to determine total bacterial contamination, Coliform, Escherichia coli, and Salmonella sp. on broiler chicken meat in Badung Regency and determine the suitability of broiler chicken meat samples following food safety standards. Samples were taken using a purposive sampling method in six sub-districts in Badung Regency, namely Petang, Abiansemal, Mengwi, North Kuta, Kuta and South Kuta sub-districts at traditional markets that sell broiler chicken meat. The samples were tested using the pour plate and streak platting method. Parameters tested include Total Plate Number (ALT), Coliform, Escherichia coli, and Salmonella sp. on broiler chicken meat samples. Coliform and Escherichia coli bacteria were detected using Petrifilm ready-to-use media, while Salmonella sp. tested on selective Salmonella-Shigella Agar (SSA) media and catalase test and Gram staining. The research result showed that the contamination rates in broiler chicken breast meat were: Total Plate Number (ALT) 105 CFU/g to 107 CFU/g; Coliform and Escherichia coli 101 CFU/g to 104 CFU/g; Salmonella sp. positive in all samples, so that the samples tested did not meet the safety quality requirements for chicken meat according to SNI 3924: 2009.

Deteksi Cemaran Total Bakteri, Coliform, Escherichia coli, dan Salmonella sp. pada Daging Ayam Broiler (Gallus domesticus) yang Dijual di Beberapa Pasar Daerah Kabupaten Badung, Bali Kadek Aryndha Sukma Prabaswari; Retno Kawuri; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 2 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i02.p4

Chicken meat is a source of animal protein which is easily damaged due to its high water content. Chicken meat that is bought and sold could potentially become a place for the growth of bacteria that cause foodborne diseases.This study aims to determine total bacterial contamination, Coliform, Escherichia coli, and Salmonella sp. on broiler chicken meat in Badung Regency and determine the suitability of broiler chicken meat samples following food safety standards. Samples were taken using a purposive sampling method in six sub-districts in Badung Regency, namely Petang, Abiansemal, Mengwi, North Kuta, Kuta and South Kuta sub-districts at traditional markets that sell broiler chicken meat. The samples were tested using the pour plate and streak platting method. Parameters tested include Total Plate Number (ALT), Coliform, Escherichia coli, and Salmonella sp. on broiler chicken meat samples. Coliform and Escherichia coli bacteria were detected using Petrifilm ready-to-use media, while Salmonella sp. tested on selective Salmonella-Shigella Agar (SSA) media and catalase test and Gram staining. The research result showed that the contamination rates in broiler chicken breast meat were: Total Plate Number (ALT) 105 CFU/g to 107 CFU/g; Coliform and Escherichia coli 101 CFU/g to 104 CFU/g; Salmonella sp. positive in all samples, so that the samples tested did not meet the safety quality requirements for chicken meat according to SNI 3924: 2009.

DETEKSI CEMARAN TOTAL BAKTERI DAN IDENTIFIKASI Salmonella sp. PADA TELUR GARAM YANG DIJUAL DI PASAR TRADISIONAL DI DENPASAR BALI : DETECTION OF TOTAL BACTERIAL CONTAMINATION AND IDENTIFICATION OF Salmonella sp. ON SALT EGGS SOLD AT THE TRADITIONAL MARKET IN DENPASAR BALI Dimung, Marieta Demarilac; Parwanayoni, Ni Made Susun; Yulihastuti, Dwi Ariani
SIMBIOSIS Simbiosis Volume 13 No. 2 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i02.p07

ABSTRACT Salt eggs are a type of egg that undergoes a five-hour boiling process and then coated with salt. Eggs are subject to damage, both from external sources and during microbial development. One of the microorganisms that contaminate eggs is Salmonella sp. This study aims to identify Salmonella sp. and examine the detection of total bacterial contamination in salted eggs sold in traditional markets in Denpasar Bali. Samples of this study were taken from 10 traditional markets located in Denpasar Bali randomly and three times repeated. The type of research conducted is descriptive research which describes the total bacteria in salt eggs and the presence or absence of Salmonella sp. The data collected is displayed using tables, figures, and in-depth explanations based on the findings of relevant literature reviews. Based on the results of the Alt test, it shows that at a dilution of 10-3 salt egg samples from Peguyangan and Kertha Boga Pemogan markets have more than 300 colonies (TBUD). This number still meets the SNI requirements, which is a maximum of 1 x 105 CFU. The results of the Salmonella sp. bacteria detection test are that all salt egg samples are free of Salmonella sp. bacteria.

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