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All Journal Jurnal Veteriner Majalah Obstetri dan Ginekologi Biomolecular and Health Science Journal Indonesian Andrology and Biomedical Journal JIMKI: Jurnal Ilmiah Mahasiswa Kedokteran Indonesia

Aswin, R. Haryanto

Departemen Biologi Kedokteran, Fakultas Kedokteran, Universitas Airlangga, Surabaya

Author-ID : 2938341

Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Veterinary

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Hyaluronan Expression on Vitrified Oocytes Before and After In Vitro Maturation (EKSPRESI HYALURONAN PADA OOSIT YANG DIVITRIFIKASI SEBELUM DAN SESUDAH MATURASI IN VITRO) Zakiyatul Faizah; R. Haryanto Aswin; Hamdani Lunardhi; Widjiati Widjiati
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Oocyte vitrification is a major challenge in assisted reproductive technology. Oocyte vitrification with cumulus cells provide benefits in the process of maturation and fertilization. Vitrification leads to rapid temperature changes, therefore the decreasing in temperature could damage the cells even when the morphology was normal. Vitrification of mature oocytes is common because of its low sensitiveness towards low temperatures than immature oocytes. The aim of the research was to compare the effect of vitrification before and after in vitro maturation to the expression of hyaluronan. Maturation was operated in medium TC 50 ?L in CO2 incubators for 24 hours. Vitrification started with washing oocyte in PBS basic medium supplemented with 20% serum for 1-2 minutes, then in equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing was processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose (K1); 2) PBS + 20% serum + 0.25 M sucrose (K2); and 3).PBS + 20% serum + 0.1 M sucrose (K3). Immunocytochemical stain was performed to evaluate the hyaluronan expression. Remmele scale index (Immunoreactive score, IRS) was used to read the result. There was no differences of hyaluronan expression in oocyte and cumulus group of K1, K2 and K3 at p< 0.05, statistically. We concluded that there was no difference of hyaluronan expression on oocyte and cumulus between vitrified oocyte of pre and post in vitro maturation which indicated that oocyte could be vitrified in the immature and mature state.

Ekspresi Transforming Growth Factor-beta dan Growth Differentiation Factor-9 Oosit Domba yang Divitrifikasi Sesudah dan Sebelum Maturasi In Vitro Zakiyatul Faizah; Raden Haryanto Aswin
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Oocyte vitrification today became a hope to preserve fertility. Its was a major challenge because of oocyte characteristic in every phase. Immature oocytes were more sensitive to osmotic stress and the membrane wes less stable while mature oocyte have spindles that were very susceptible to temperature decrease. The study aim to compare the effect of vitrification before and after in vitro maturation to the expression TGF beta and GDF9. Oocyte of ewes divided into control groups (K0), K1 maturation prior vitrification, K2 vitrification prior maturation. Vitrification begins with washing oocytes in PBS supplemented of 20%serum for 1-2 minutes, followed by equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing was processed by in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2).PBS + 20% serum + 0.25 M sucrose, and 3).PBS + 20% serum + 0.1 M sucrose. Immunocytochemical stain was performed to evaluate TGF ? and GDF9 expression. Remmele scale index (IRS) was used to read the result. TGF beta expression both in oocyte and cummulus of K0 and K1 was significant statistically difference (p<0.05) compare with K2. GDF9 expression both in oocyte and cummulus of K0 and K1 was significant statistically difference (p<0.05) compare with K2. We concluded that immature oocyte give better expression of TGF â and GDF9 than mature oocyte.

Co-Authors Berliana Hamidah Faisal Yusuf Ashari Faizah, Zakiyatul Hamdani Lunardhi Izdihar Ramadhanty Abiyasa Putri Izzatul Fithriyah Judie Hartono Judie Hartono Muhammad Miftahussurur Natasya Ariesta Selyardi Putri Ninik Darsini Relly Yanuari Primariawan, Relly Yanuari RINA YUDIWATI BM, RINA Seso Sulijaya Suyono Tri Hartini Yuliawati Ummi Maimunah Widjiati Widjiati Williana Suwirman Williana Suwirman Yoshio Yamaoka

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