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All Journal Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Entomologi Indonesia Berkala Penelitian Hayati Nandur
MADE SRITAMIN
Program Studi Agroekoteknologi Pertanian Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : Nandur

 1 
Isolasi dan Skrining Bakteri Selulolitik pada Feses Luwak I Putu Eko Arymanto Wira Kesuma; I Nyoman Wijaya; Made Sritamin
Nandur Vol 1 No 3 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

Civet (Paradoxurus hermaphroditus) are carnivorous animals, but also often eat fruit and one of them is coffee cherries. The coffee cherries that are eaten are not completely digested, so the coffee beans come out with the civet feces. In the civet digestion there are various kinds of bacteria that help the digestive process, one of them is cellulolytic bacteria. It is possible that coffee beans in civet feces contain cellulolytic bacteria.This study aims to determine the presence of cellulolytic bacteria in civet feces. The results of bacterial isolation in civet feces obtained 6 isolates that had different morphological characters. Cellulolytic bacteria screening results showed that 2 out of 6 isolates were positive as cellulolytic bacteria, namely FL2 isolate with a cellulolytic index of 2.38 (high reaction) and FL3 isolate with a cellulolytic index of 1.24 (medium reaction). The isolates FL2 and FL3 had different morphological characters in colony shape, margins and color, but had similarities in elevation, gram color and cell shape.
Identifikasi Senyawa Fitokimia pada Daun Kersen (Muntingia calabura Linn.) dan Uji Toksisitasnya Terhadap Larva Udang Artemia salina Leach Ade Febrianto Umbu Romu Kolambani; Made Sritamin; I Nyoman Wijaya
Nandur Vol 1 No 4 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Singapore cherry (Muntingia calabura L.) is a plant that has shady trees, is often used as a shade and is easy to grow in various places. All parts of the cherry plant, including roots, bark, fruit, and leaves, contain secondary metabolites that can be used as traditional medicine. The purpose of this study was to determine the phytochemical compounds contained in cherry leaf extract and to determine the level of acute toxicity (LC50) using the Brine Shrimp Lethality Test (BSLT) method. Identification of phytochemical compounds was carried out by gas chromatography-mass spectrometry and acute toxicity test (LC50) was carried out using the BSLT method using the shrimp larvae test insect Artemia salina Leach. The acute toxicity test (LC50) consisted of 6 concentration treatments, namely 500 ppm, 250 ppm, 150 ppm, 100 ppm, 50 ppm, along with negative controls, each of which was repeated three times. The LC50 value is obtained from probit analysis in Microsoft Office Excel. There are 3 groups of bioactive compounds with the highest peak area in singapore cherry leaf extract, namely groups of flavonoids, phenols, and alkaloid. The result of acute toxicity test (LC50) of singapore cherry leaves extract was 84,029 ppm.
Studi Komparasi Jenis Pelarut Ekstraksi terhadap Kandungan Fitokimia Daun Tanaman Kubis Ungu Hengky Surya Bhuana; Made Sritamin; I Gede Putu Wirawan
Nandur Vol 1 No 4 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Red cabbage is a plant that is rich in antioxidant, one of its secondary metabolites, antosianin that gave the purple color on red cabbage has multiple health effects for human such as preventing cancer, tumor, and many more. The use of this secondary metabolites in plants nowadays is very popular, not only for its pharmacological effects, but also as an antibacterial, coloring agent, flavoring agent, natural pesticide, etc. Therefore, this study is needed to look for another potentially benefical compound in the plant, such as red cabbage, a plant that is more than just a salad. The aim of this study was to determine the effect of the types of extraction solvents on phytochemical compound of red cabbage plant leaves. The dried off plant macerated with two kinds of solvents, aquades, and ethanol. After about +3 days, both of the extract got evaporated with rotary evaporator. The yields of evaporating will be tested with GCMS analysis. The result showed that kind of extraction solvents do affect the yield from evaporating, and GC-MS analysis. Aquades showed to have a better capability in both of extraction, or on the analysis with GC-MS.
Isolasi dan Identifikasi Bakteri Selulolitik pada Buah Kopi Arabika (Coffea arabica L.) Nona Au Waling; Made Sritamin; I Nyoman Wijaya
Nandur Vol 1 No 3 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Cellulolytic bacteria are microorganisms that can produce cellulase enzymes. Cellulolytic bacteria contain cellulase enzymes that play a role in the cellulose degradation processes. The purpose of this study was to isolate cellulolytic bacteria that capable to degrading cellulose from arabica coffee cherries collected from Catur Village, Kintamani. The results of bacterial isolation at 10-6 and 10-8 dilutions obtained 10 bacterial colonies. From the results of bacterial purification, 4 different bacterial isolates had different morphological characters. The results of the isolation of the colonies showed differences in a round and irregular shape, had raised and convex surfaces had red, cream, white and yellow colors, had curved and smooth edges. Tests were carried out on four bacterial isolates to determine the clear zone index. Only the L isolate in the congo red test had a diameter of clear zone 2.5 mm and with score 3 based on cellulolytic index value L isolate categorized as strong degrading cellulose bacteria.
Pembentukan Mutan Hipovirulen Fusarium oxysporum f.sp cubense Penyebab Layu pada Tanaman Pisang (Musa paradisiaca L.) Menggunakan Asam Nitrat Fransen Sinaga; Made Sritamin; I Ketut Suada
Nandur Vol 1 No 1 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

The Formation of Fusarium oxysporum f.sp. cubense Hypovirulent Mutant Causes Wilt in Banana Plants (Musa Paradisiaca L.) Using Nitric Acid. Fusarium wilt disease is a type of disease that can cause a quantitative decrease in banana plant production. Fusarium wilt disease is classified as a disease that is difficult to control, because it is underground and forms chlamydospores. Control of Fusarium with biological agents has a positive impact on the environment, one way of biological control using antagonistic microorganisms. Biological agents used are biological agents that are hypovirulent. Hypovirulent properties will compete with host pathogens in competition for space, food, and toxin excretion, thereby suppressing fusarium fungi to enter the vascular tissue of banana plants. Chemical mutagens are mutagenic agents that can cause permanent changes in the composition of DNA. Nitric acid can cause DNA cross-linking in the same strands. Nitric acid was reported as a suitable mutagen to increase the production of lactic acid Lactobacillus delbrueckii. Nitric acid can change the acid-base pair of organisms so that mutations occur. obtained a mutant that is hypovirulent which can suppress the growth of fusarium fungus as a pathogen in banana plants. The results showed that the FOC fungus isolates with 1% treatment had hypovirulent properties compared to 0.05%, 0.1% and 0.5% treatments. Hypovirulent properties are derived from the timing of symptoms. In mutant FOC, 1% of symptoms appeared on day 6. The growth of hypovirulent mutants was expected to be higher than that of pathogenic FOCs. Based on the research, it was found that the fungi had the lowest pathogenicity level, as well as high diameter growth, total spore density, and high amount of inhibition.
Co-Authors Abd. Rasyid Syamsuri Ade Febrianto Umbu Romu Kolambani ANAK AGUNG GEDE GARBA YOGANTARA Aulanni ‘am Aulanni ‘am BAYU ARYA BAGASKARA CATUR KOESUMA WARDHIANY DARWIN SILALAHI DELVIANA PANJAITAN DEWA AYU RATIH DIMINDA ELA SRI ERTINA BR GINTING EKA BHUWANA BONAULI BARITA Evy Prima Fransen Sinaga Gresia Eldina GUSTI PUTU DINTYA PUTRA Haryadi, Celvin Hengky Surya Bhuana I DEWA PUTU SINGARSA I G. N. Bagus I G. Ngurah Bagus I G.N. Bagus I Gede Ketut Susrama I GEDE PUTU WIRAWAN I Gusti Ayu Diah Yuniti I Gusti Ngurah Bagus I Ketut Suada I KETUT SUMIARTHA I M. Sudarma I Made Mega Adnyana I N. Wijaya I N. WIJAYA I NYOMAN TRYADI CAHYA NUGRAHA I NYOMAN WIJAYA I Putu Eko Arymanto Wira Kesuma I. G. N Bagus I. N. Wijaya IDA BAGUS MADE DWI JAYA KATARINA IKA NOVIANITA KETUT AYU YULIADHI Liliek Sulistyowati LUH PUTU SUGIARI M Sudarma M. Puspawati Made Adnyana MAGNA DWIPAYANA MUSLIM MUSLIM NI KADEK BUDARTINI NI KADEK DWI PASARI Ni Kadek Emi Sintha Dewi Ni Kadek Sintya Dewi NI KADEK SINTYA DEWI NI LUH MADE PRADNYAWATHI Ni Made Puspawati NI MADE PUTRI YUDANTARI Nona Au Waling NURUL KHOTIMAH PUTU ANA DIANTARI PUTU AYU MEITA YUDIA DEWI PUTU BAMBANG IKA WINJALISTA PUTU PADMA MAHESWARI Rindang Dwiyani RISMA IMROATUS SHOLIHAH Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sasadara, Maria Malida Vernandes Shara Yulita Harianja SHEILA SIMANJUNTAK SUNARTI TAMBUNAN TRIAN WANGSA PURWANTO TRISNA AGUNG PHABIOLA VLORA VERONICA SIPANGKAR WAYAN ADIARTAYASA Wijaya I N. Yundari, Yundari