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Journal : Agrotrop : Journal on Agriculture Science

Pengaruh Kehadiran Gulma terhadap Jumlah Populasi Hama Utama Kubis pada Pertanaman Kubis KETUT AYU YULIADHI; TRISNA AGUNG PHABIOLA; MADE SRITAMIN
Agrotrop : Journal on Agriculture Science Vol 3 No 1 (2013)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (36.78 KB)

Abstract

The Influence of The Presence of Weeds on The Population Number of main Pest of Cabbage on The Cabbage Croppings The aim of the research was to find out the development of population number of Plutella xylostella and Crocidolomia pavonana in the precence and the absence of weeds on the cabbage croppings. The results showed that there were 23 species of weeds in association with plant cabbage, which was made up of 7 species of grasses, 3 species of weeds from Cyperaceae family, and 13 species of broadleaved weeds. Main pests of cabbage on cabbage croppings (Plutella xylostella and Crocidolomia pavonana) was found more on cabbage plants in the absence of weeds compared than that of the presence of weeds.
Perbanyakan Tanaman Jeruk Siam (Citrus nobilis L.) dengan Teknik Kultur in vitro Menggunakan Biji Tanaman Terinfeksi Penyakit Citrus Vein Phloem Degeneration (CVPD) BAYU ARYA BAGASKARA; I GEDE PUTU WIRAWAN; MADE SRITAMIN; I GUSTI AYU DIAH YUNITI
Agrotrop : Journal on Agriculture Science Vol 8 No 2 (2018)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.164 KB) | DOI: 10.24843/AJoAS.2018.v08.i02.p09

Abstract

Citrus (Citrus nobilis L.) Propagation Through Culture In Vitro Using Seed From Infected Plants Of Citrus Vein Phloem Degeneration (CVPD) Disease. The sample was taken in Belancan Village, Kintamani District, Bangli Regency, and invitro culture was conducted in UPT. Genetics Resource and Molecular Biology Laboratory in February to June 2018. This research purposes were to get the new citrus plant from citrus seed infected of CVPD disease explants with culture in vitro and found the presence of the bacterium Liberobacter asiaticus in results of citrus plants culture in vitro. The sampling method is using plants appearance. Obtained sample 1 is a plant that shows severe symptoms of CVPD disease and sample 2 plants that show no symptoms of CVPD disease. Seed Sample is cultured with MS0 medium for 8 WAP then proceed to acclimatization. The results of this research showed that the seed explants from both samples were able to grow well and did not have the characteristic of CVPD disease, but explant seed sample 2 in vitro culture result showed that the bacterial DNA bacterium Liberobacter asiaticus pathogen CVPD disease does exist in length 1160bp, so it cannot be expected to produce CVPD-free crops.
Transformasi Genetik Tanaman Kentang (Solanum tuberosum L.) dengan Gen acvB Menggunakan Vektor Agrobacterium tumefaciens DARWIN SILALAHI; I GEDE PUTU WIRAWAN; MADE SRITAMIN
Agrotrop : Journal on Agriculture Science Vol 11 No 1 (2021)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2021.v11.i01.p07

Abstract

Agrobacterium tumefaciens Mediated Genetic Transformation of acvB Gene in Potato (Solanum tuberosum L.). Genetic transformations are now routinely applied to plant mediated by Agrobacterium tumefaciens as the most convenient technique. This study aimed to prove the success of A. tumefaciens mediated genetic transformation in potato. A. tumefaciens LBA (pBI 121) and explant of potato shoot were used in this study. Explants were grown in vitro on Murashige and Skoog media. Transformation was implemented using smear technique by smearing A. tumefaciens to injured explant. Experimental groups consisted of two groups: control group which did not receive transformation treatment and treatment group receiving transformation treatment. Explant growth was observed through the presence of shoots, branches and the shoot height. Explants in the treatment group resulted in a higher number of shoots, branches, and shoot heights compared to control. Phenol compounds appear in explant epidermal tissue, indicating the wounds produced by A. tumefaciens infection, thus the gene predicted to be transformed. Identification by PCR is needed to prove the existence of the acvB gene in potato plants genome, using acvB specific PCR primer as the marker, such as (5?-CCCT CTAG AGAC CCGC GCCA AGGCG-3?) and (5?CGCG TCGA CCTT GTCG GAAAG -3?) with 540-bp in base pair size produced.
Kultur Jaringan Jeruk Keprok Tejakula (Citrus reticulata var. Tejakula) Menggunakan Tunas Muda dan Biji Serta Deteksi CVPD dengan Teknik Polymerase Chain Reaction (PCR) VLORA VERONICA SIPANGKAR; I NYOMAN WIJAYA; MADE SRITAMIN
Agrotrop : Journal on Agriculture Science Vol 10 No 1 (2020)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.488 KB) | DOI: 10.24843/AJoAS.2020.v10.i01.p06

Abstract

Tissue Culture Citrus (Citrus reticulata var. Tejakula) using Shoot and Seed and the Detection of CVPD with Polymerase Chain Reaction (PCR). The sample was taken in Pecatu Village, Kuta Selatan District, Badung Regency and continued with in vitro culture and PCR analysis at UPT. Genetic Resource and Molecular Biology Laboratory, was start from December of 2018 until May of 2019. The purpose of this research was to create a CVPD free Citrus reticulata var. Tejakula seedling from tissue culture. The sample was chosen based on visual characteristic. Two samples were taken from citrus plant that show CVPD symptoms and citrus plant that did not show CVPD symptoms. The seed and shoot were cultured using MS media for 12 week after planting (WAP). From this research showed that both explant from the shoot and seed were able to grow well. The explant from citrus sample that showed CVPD symptoms was infected by Liberibacter asiaticus and the explants from citrus that did not show CVPD symptoms was not invected by Liberibacter asiaticus, according to PCR analysis.
Identifikasi Senyawa Fitokimia dan Uji Aktivitas Antioksidan Ekstrak Etanol Limbah Kulit Biji Kakao (Theobroma cacao L.) Varietas Trinitario Haryadi, Celvin; Wirawan, I Gede Putu; Sritamin, Made
Agrotrop : Journal on Agriculture Science Vol 13 No 3 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i03.p03

Abstract

Identification of Phytochemical Compounds and Antioxidant Activity Test of Ethanol Extract of Cocoa Bean Shell Waste (Theobroma cacao L.) Trinitario Varieties. Cocoa is one of the mainstay commodities of plantations in Indonesia, contributing to the country's foreign exchange earnings. Byproducts such as fruit skins and pulp are produced during the production of dry cocoa beans, and waste in the form of cocoa bean husks is produced during the processing of dry cocoa beans into chocolate products. So far, cocoa bean husk waste has not been used optimally, leading to a low economic value. The purpose of this study was to determine the bioactive compounds in the skin and the levels of antioxidant compounds in cocoa bean husk waste so that it could be used as a raw material for medicine. The GC-MS analysis method was used to identify bioactive compounds in cocoa bean husk waste, and the DPPH method was used to test antioxidant activity. According to the results of the GC-MS analysis, the cocoa bean husk waste extract contains four compounds with quality value higher than 90. These four compounds are Alpha-Copaene, Copaene, Caryophyllene, and Caffeine. The antioxidant test results of the cocoa bean husk waste extract revealed an IC50 value of 86,213 ppm, indicating strong antioxidant activity because the IC50 value is between 50-100 ppm.
Co-Authors Abd. Rasyid Syamsuri Ade Febrianto Umbu Romu Kolambani ANAK AGUNG GEDE GARBA YOGANTARA Aulanni ‘am Aulanni ‘am BAYU ARYA BAGASKARA CATUR KOESUMA WARDHIANY DARWIN SILALAHI DELVIANA PANJAITAN DEWA AYU RATIH DIMINDA ELA SRI ERTINA BR GINTING EKA BHUWANA BONAULI BARITA Evy Prima Fransen Sinaga Gresia Eldina GUSTI PUTU DINTYA PUTRA Haryadi, Celvin Hengky Surya Bhuana I DEWA PUTU SINGARSA I G. N. Bagus I G. Ngurah Bagus I G.N. Bagus I Gede Ketut Susrama I GEDE PUTU WIRAWAN I Gusti Ayu Diah Yuniti I Gusti Ngurah Bagus I Ketut Suada I KETUT SUMIARTHA I M. Sudarma I Made Mega Adnyana I N. WIJAYA I N. Wijaya I NYOMAN TRYADI CAHYA NUGRAHA I NYOMAN WIJAYA I Putu Eko Arymanto Wira Kesuma I. G. N Bagus I. N. Wijaya IDA BAGUS MADE DWI JAYA KATARINA IKA NOVIANITA KETUT AYU YULIADHI Liliek Sulistyowati LUH PUTU SUGIARI M Sudarma M. Puspawati Made Adnyana MAGNA DWIPAYANA MUSLIM MUSLIM NI KADEK BUDARTINI NI KADEK DWI PASARI Ni Kadek Emi Sintha Dewi Ni Kadek Sintya Dewi NI KADEK SINTYA DEWI NI LUH MADE PRADNYAWATHI Ni Made Puspawati NI MADE PUTRI YUDANTARI Nona Au Waling NURUL KHOTIMAH PUTU ANA DIANTARI PUTU AYU MEITA YUDIA DEWI PUTU BAMBANG IKA WINJALISTA PUTU PADMA MAHESWARI Rindang Dwiyani RISMA IMROATUS SHOLIHAH Sang Ayu Made Putri Suryani Sang Ayu Made Putri Suryani, Sang Ayu Made Putri Sasadara, Maria Malida Vernandes Shara Yulita Harianja SHEILA SIMANJUNTAK SUNARTI TAMBUNAN TRIAN WANGSA PURWANTO TRISNA AGUNG PHABIOLA VLORA VERONICA SIPANGKAR WAYAN ADIARTAYASA Wijaya I N. Yundari, Yundari