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PENGEMBANGAN DAN VALIDASI METODE KROMATOGRAFI LAPIS TIPIS DENSITOMETRI UNTUK PENETAPAN KADAR TEOFILIN DAN EFEDRIN HIDROKLORIDA SECARA SIMULTAN PADA SEDIAAN TABLET Lestyo Wulandari; Yuni Retnaningtyas; Diyanul Mustafidah
Jurnal Kimia Terapan Indonesia Vol 15, No 1 (2013)
Publisher : Research Center for Chemistry - LIPI

The combination of Theophylline and Ephedrine isoften used to induce an additive effect in alleviating thesymptoms of respiratory disorders. This research is aimed todevelop a Thin Layer Chromatography (TLC) densitometrymethodfor the simultaneous determination of Theophyllineand Ephedrine hydrochloride in tablet dosage form. Themethod employed TLC aluminium plates precoated withsilica gel 60F254 as the stationary phase and the mixture ofethyl acetate: glacial acetic acid: aquabidest (11: 5 : 1 v/v)asthe eluen system. For detection of Ephedrinehydrochloride, the TLC plate must be re-eluted with 0,5%ninhydrin solution in aquabidest, and then heated at 105Â°Cfor 15 min. Quantitative evaluation was performed bymeasuring the absorbance-reflectance of the analyte spot at279 nm and 505 nm for Theophylline and Ephedrinehydrochloride, respectively. validation of the method showthat TLC Densitometryis selective and specific,showed agood linearity, precise, and accurate and can be used forroutine analysis of tablet in industrial quality controllaboratories.Keywords:TLCDensitometryV, alidationT, heophyllineE, phedrinehydrochloride

Penggunaan Indikator Film Edible berbasis Antosianin Hibiscus rosa-sinensis L untuk Monitoring Kesegaran Tomat Ceri Dewi Enggar Fitriani; Bambang Kuswandi; Lestyo Wulandari
Pustaka Kesehatan Vol 10 No 1 (2022): Volume 10 No.1, 2022
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v10i1.12616

Fruit and vegetable are food products that are in great demand by the public. One of the fruit and vegetable products that can be enjoyed by the community is cherry tomatoes. Therefore, they required for quality of cherry tomato products, in this regard, the method was needed to determine the freshness of cherry tomatoes easily and practically. The purpose of this study was to develop an edible freshness sensor based on anthocyanin of shoe flower extract (Hibiscus rosa-sinensis L) with edible membrane from a mixture of chitosan and rice starch. The edible freshness sensor can be applied as a freshness sensor and determine the freshness level of cherry tomatoes with various parameters. The freshness evaluation of cherry tomatoes included weight loss test, pH, texture, and organoleptic test. The color change of the edible freshness sensor can be detected by the nude eye and analyzed by using ImageJ program to determine the value of the mean red of RGB. The results showed the color change of the freshness sensor which was dark grey when the cherry tomatoes were fresh, dark purple when the cherry tomatoes were still fresh and reddish-purple when cherry tomatoes were no longer fresh. The value of mean red from the edible freshness sensor increase with decreasing the freshness level of cherry tomatoes

Uji aktivitas antibakteri ekstrak etanol dan fraksi daun gempol (Nauclea orientalis L.) terhadap bakteri Escherichia coli dan Staphylococcus aureus Nia Kristiningrum; Reny Diastri Noviriana; Lestyo Wulandari
Jurnal Ilmiah Farmasi Vol. 18 No. 1 (2022): Jurnal Ilmiah Farmasi
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.vol18.iss1.art1

Background: The leaves of gempol (N. orientalis L.), a family of Rubiaceae, have antibacterial activity. Objective: The aim of this research was to determine the antibacterial activity of gempol leaf ethanolic extract and its fractions. Methods: The antibacterial activity test was carried out using the disk diffusion method. The positive and negative controls used were a disk of 10 μg gentamicin and 10% DMSO solution, respectively. The test solution concentrations for each sample, including for the ethanolic extract, hexane fraction, ethyl acetate, and residue of gempol leaves, were 0.25%, 0.5%, 1%, 2%, and 5%. Results: The antibacterial activity of the ethanolic extract, hexane fraction, ethyl acetate fraction, and residue of gempol leaves against E. coli and S. aureus was significantly different from each other. The residue had the highest antibacterial activity followed by that of the ethyl acetate fraction, hexane fraction, and ethanolic extract. Conclusion: The antibacterial activity of gempol leaves against S. aureus was greater than against E. coli.Keywords: Gempol, Nauclea orientalis, extract, fractions, antibacterial, diffusion IntisariLatar Belakang: Daun gempol (N. orientalis L.) merupakan salah satu famili Rubiaceae yang telah diketahui memiliki aktivitas anthelmintik dan antibakteri.Tujuan: penelitian ini adalah untuk mengetahui aktivitas antibakteri berdasarkan zona hambat ekstrak etanol dan fraksi daun gempol.Metode: yang digunakan ialah metode difusi cakram yang menghasilkan diameter zona hambat sebagai hasil dari uji aktivitas antibakteri. Kontrol positif yang digunakan yaitu cakram gentamisin 10 μg sedangkan kontrol negatif yang digunakan yaitu DMSO 10%. Larutan uji meliputi ekstrak etanol, fraksi heksana, etil asetat, dan residu daun gempol dengan masing-masing konsentrasi yaitu 0,25%; 0,5%; 1%; 2%; dan 5%.Hasil: Aktivitas antibakteri dari ekstrak etanol, fraksi heksan, etil asetat dan residu daun gempol terhadap E. coli dan S. aureus menunjukkan perbedaan yang signifikan. Residu memiliki aktivitas antibakteri tertinggi diikuti oleh fraksi etil asetat, fraksi heksana dan ekstrak etanol.Kesimpulan: Aktivitas antibakteri pada S. aureus lebih besar dari pada E. coli.Kata kunci: Gempol, Nauclea orientalis, ekstrak, fraksi, antibakteri, difusi

AKTIVITAS ANTIOKSIDAN FRAKSI AIR DAN FRAKSI ETER KOMBINASI EKSTRAK METANOL DAUN KOPI ARABIKA (Coffea arabica L.) DAN KULIT BATANG KAYU MANIS (Cinnamomum burmannii Nees ex BI.): Antioxidant Activity of Water and Ether Fraction Combination of Arabica Coffee Leaves (Coffea arabica L.) Extract and Cinnamon burmanii Bark (Cinnamomum burmannii Nees ex Bi.) Retnaningtyas, Yuni; Wulandari, Lestyo; Fauzan Arrozi
Jurnal Tumbuhan Obat Indonesia Vol. 16 No. 1 (2023): July 2023
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jtoi.v16i1.604

Antioxidants are compounds that can inhibit an oxidation reaction, by binding to free radicals that play a role in the emergence of several degenerative diseases. Arabica coffee (Coffea arabica) and cinnamon (Cinnamomum burmannii) are plants that have antioxidant activity so that when combined they have the potential to produce a synergistic effect. The purpose of this study was to test the antioxidant effects of the combination of methanol extract of Arabica coffee leaves and cinnamon bark in both the water and ether fractions. Antioxidant activity was determined using the DPPH method, as a positive control vitamin C was used, and antioxidant activity was expressed in the form of IC50. The results showed a synergistic effect of the two extracts where the antioxidant activity of both the water fraction and the ether fraction in the combined form was greater than the single form. The highest antioxidant activity was shown by the water fraction of the combination of methanol extract of Arabica coffee leaves and cinnamon bark in a ratio (1:2) with an IC50 value of 8.759 ± 0.050 g/mL. This antioxidant activity is lower than the antioxidant activity of vitamin C with an IC50 value of 3.267 ± 0.007 g/mL

Antioxidant Assay and Total Flavonoid Determination of Ethanolic Extract of Walnut (Canarium indicum L.) Leaves and Its Fractions Lukmanto, Lukmanto; Fadhila, Nadila Ikhda Ramadhani; Wulandari, Lestyo; Puspitasari, Endah
Jurnal ILMU DASAR Vol 24 No 1 (2023)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v24i1.23017

Antioxidants are compounds that inhibit free radicals. Many plants have antioxidant activity because they contain antioxidant compounds such as flavonoids. This study aimed to evaluate the antioxidant activity, phytochemical contents, total flavonoid content and to establish the relationship of total flavonoid content and antioxidant activity of extract and fractions of walnut (Canarium indicum L.) leaves. Antioxidant activity of ethanolic extract, n-hexane fraction, ethyl acetate fraction, and ethanolic-water fraction of walnut leaves was examined by using the DPPH method and determination of total flavonoid content was determined spectrophotometrically. The results showed that antioxidant activity of ethanolic extract, n-hexane fraction, ethyl acetate fraction, and ethanolic-water fraction based were 25.294 ± 0.055; 175.245 ± 0.4999; 20.135 ± 0.009; and 28.806 ± 0.0424 μg/ml, respectively. The phytochemical content of ethanol extract of walnut leaves are saponins, flavonoids, tannins, and polyphenols. The total flavonoid content of ethanolic extract, n-hexane fraction, ethyl acetate fraction and ethanolic-water fraction were 2.624 ± 0.012; 0.499 ± 0.023; 3.846 ± 0.006; and 1.596 ± 0.006 gram quercetin equivalent/gram extract, respectively. Correlation between antioxidant activity and total flavonoid content revealed that 63.2 % of antioxidant activity was influenced by the presence of flavonoid compounds.

Penentuan Aktivitas Antioksidan dan Antidiabetes Ekstrak Daun Matoa (Pometia pinnata J.R. Forst. & G. Forst.) secara In Vitro Wulandari, Lestyo; Nugraha, Ari Satya; Himmah, Ulfa Aliyatul
Jurnal Kefarmasian Indonesia VOLUME 11, NOMOR 2, AGUSTUS 2021
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v11i2.3196

Matoa (Pometia pinnata J.R. Forst. & G. Forst.) Matoa (Pometia pinnata J.R. Forst. & G. Forst.) is one of the plants that is used as a traditional medicine for diabetes mellitus due to an imbalance between the amount of ROS and antioxidants in the body. Therefore, it was carried out in vitro to see the antioxidant and antidiabetic activity in matoa leaf extract. The extraction of matoa leaves was carried out using the ultrasonication method for 30 minutes with methanol, ethanol, and ethyl acetate as solvents. Antioxidant activity is release through DPPH free radical inhibition, through the antidiabetic potential released by inhibiting the work of the α-amylase enzyme. Phytochemical test results showed the presence of secondary metabolites in the form of flavonoids, polyphenols, tannins, alkaloids, and terpenoids. The results of the research on methanol, ethanol, and ethyl acetate extracts of matoa leaves showed high antioxidant activity with IC50 values of 6.416 ± 0.176 ppm, 8.622 ± 0.066 ppm, and 170.637 ± 4.441 ppm, respectively, but they were less potent than vitamin C as a comparison which is 1.646 ± 0.015 ppm. Inhibition of the α-amylase enzyme showed IC50 values of 91.037 ± 0.750 ppm, 105,166 ± 2,423 ppm, and 785,436 ± 11,740 ppm in each of the methanol, ethanol, and ethyl acetate extracts while the IC50 value of acarbose as a comparison was 23,479 ± 0.347 ppm. The statistical data analysis of Pearson correlation showed that it had a positive relationship between the antioxidant and antidiabetic activity of matoa leaf extract as seen from the R-value of 0.998. The higher antioxidant activity, so the higher potential for inhibition of α-amylase enzyme.

Determination of Total Citronelal Levels and Development of FTIR-Chemometric Classification Model of Cymbopogon winterianus Jowitt Oil from Different Altitudes of Planting Areas Jazila, Yusrin; Kristiningrum, Nia; Wulandari, Lestyo
Jurnal Jamu Indonesia Vol. 8 No. 1 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i1.279

Cymbopogon winterianus Jowitt oil (CWJ oil) is a vegetable oil with very wide uses in food sector, cosmetics, medicines and chemical industry. Citronelal compound is one of the quality requirements that determine the quality of CWJ oil. This study aims to develop a chemometric-FTIR classification model and to determine the total citronellal content of Cymbopogon winterianus Jowitt oil grown in low and medium lands. The sample used was citronella oil from Jember and Banyuwangi regions. The cleaned CWJ herbal samples were withered, chopped and distilled to produce CWJ oil. CWJ oil was scanned using FTIR spectroscopy and then the FTIR spectras were analyzed using LDA, SVM and SIMCA to form a CWJ oil classification model. The selected classification model was then validated using leave one out cross validation and external validation. The LDA classification model showed the best results with 100% accuracy of model recognition and validation. The results of the determination of total citronellal levels of CWJ oil using the hydroxylamine method showed that samples of CWJ oil from Banyuwangi had higher levels of total citronellal than samples from Jember. The average levels of total citronellal CWJ oil from Banyuwangi and Jember regions were 32.84%, and 26.06%, respectively.

Penentuan Aktivitas Antioksidan dan Antidiabetes Ekstrak Daun Kepundung (Baccaurea racemosa Muell.Arg.) secara In Vitro Wulandari, Lestyo; Nugraha, Ari Satia; Azhari, Nuri Putri
JSFK (Jurnal Sains Farmasi & Klinis) Vol 7 No 1 (2020): J Sains Farm Klin 7(1), April 2020
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.7.1.60-66.2020

Â Kepundung (Baccaurea racemosa Muell.Arg.) merupakan salah satu tanaman berkhasiat di Indonesia yang mengandung flavonoid, polifenol, tanin, dan terpenoid yang dapat berfungsi sebagai antioksidan. Kandungan senyawa dengan aktivitas antioksidan dapat dikaitkan dengan potensi aktivitas antidiabetes dari tanaman ini. Penelitian dilakukan untuk mengetahui aktivitas antioksidan dan antidiabetes ekstrak metanol, etanol, dan etil asetat dari daun kepundung. Ekstraksi dilakukan dengan metode ultrasonik. Pengujian aktivitas antioksidan dilakukan secara kuantitatif dengan metode DPPH, sedangkan pengujian aktivitas antidiabetes menggunakan metode penghambatan enzim Î±-amilase. Nilai IC50 pada penentuan aktivitas antioksidan dan antidiabetes ditentukan dengan menggunakan spektrofotometer UV-Vis pada panjang gelombang maksimum. Hasil penentuan antioksidan menunjukkan bahwa nilai IC50 ekstrak metanol, etanol, dan etil asetat masing-masing adalah 9,38 Â± 0,15; 10,55 Â± 0,09; dan 946,70 Â± 2,31 ppm. Ini menunjukkan ekstrak metanol dan etanol memberikan hasil terbaik dalam aktivitas antioksidan. Nilai IC50 aktivitas antidiabetes in vitro ekstrak metanol, etanol, dan etil asetat masing-masing adalah 67,63 Â± 0,36; 67,46 Â± 0,23; dan 841,04 Â± 1,52 ppm. Hasil ini juga menunjukkan bahwa ekstrak metanol dan etanol daun kepundung memiliki aktivitas antidiabetes yang hampir sama

Determination of Total Citronelal Levels and Development of FTIR-Chemometric Classification Model of Cymbopogon winterianus Jowitt Oil from Different Altitudes of Planting Areas Jazila, Yusrin Nur; Kristiningrum, Nia; Wulandari, Lestyo
Jurnal Jamu Indonesia Vol. 8 No. 1 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i1.279

Cymbopogon winterianus Jowitt oil (CWJ oil) is a vegetable oil with very wide uses in food sector, cosmetics, medicines and chemical industry. Citronelal compound is one of the quality requirements that determine the quality of CWJ oil. This study aims to develop a chemometric-FTIR classification model and to determine the total citronellal content of Cymbopogon winterianus Jowitt oil grown in low and medium lands. The sample used was citronella oil from Jember and Banyuwangi regions. The cleaned CWJ herbal samples were withered, chopped and distilled to produce CWJ oil. CWJ oil was scanned using FTIR spectroscopy and then the FTIR spectras were analyzed using LDA, SVM and SIMCA to form a CWJ oil classification model. The selected classification model was then validated using leave one out cross validation and external validation. The LDA classification model showed the best results with 100% accuracy of model recognition and validation. The results of the determination of total citronellal levels of CWJ oil using the hydroxylamine method showed that samples of CWJ oil from Banyuwangi had higher levels of total citronellal than samples from Jember. The average levels of total citronellal CWJ oil from Banyuwangi and Jember regions were 32.84%, and 26.06%, respectively.

Penentuan Kadar Flavonoid Total dan Klasifikasi Serbuk Daun Murbei (Morus alba L.) Berbeda Ketinggian Tempat Tanam Syaifullah, Abim; Kristiningrum, Nia; Pangaribowo, Dian Agung; Wulandari, Lestyo
Journal of Agropharmacy Vol. 1 No. 1 (2024)
Publisher : Faculty of Pharmacy, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Mulberry (Morus alba L.) is a plant from the Moraceae family which has a high flavonoid content. This research aims to determine the ability of NIR spectroscopy and chemometrics to classify mulberry leaf powder based on different planting heights and determine the total flavonoid content using the Uv-Vis spectrophotometric method. The classification of mulberry leaf powder originating from the lowlands, midlands, and highlands provides valid results using NIR spectra and chemometric data from the LDA and SIMCA models with an accuracy value of 100%. The average results of determining total flavonoid content using Uv-Vis spectrophotometry showed that the highest levels were in the highlands, followed by the medium and lowlands with total flavonoid levels respectively, namely 6.453 ± 1.194 mg QE/g powder; 5.889 ± 0.944 mg QE/g powder; and 5.177 ± 0.998 mg QE/g powder. The results of the One Way ANOVA test showed that the total flavonoid content of mulberry leaf powder from different altitude areas did not have a significant difference.

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