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All Journal Jurnal Ilmu Dasar BERKALA SAINSTEK UNEJ e-Proceeding Jurnal Kimia Riset Jurnal Biodjati Formosa Journal of Sustainable Research (FJSR) Indonesian Chimica Letters InSTEM Prosiding Seminar Nasional Pengabdian kepada Masyarakat (SINAPMAS) Journal of Bio-molecule Research And Engineering
Wuryanti Handayani
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Universitas Jember. Center For Development Of Advanced Sciences And Technology, Universitas Jember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Immunology & microbiology Industrial & Manufacturing Engineering Mathematics Physics Social Sciences Other

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Lactic Acid Bacteria Isolated From Digestive Tract of Broilers Treated with Fish Protein Hydrolysate Esti Utarti; Eva Tyas Utami; Achmad Sjaifullah; Wuryanti Handayani; Malika Belkis; Rani Dian Medayani
Jurnal Biodjati Vol 10 No 2 (2025): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v10i2.38422

Abstract

The composition of the feed plays a role in stimulating the activity of the microbiota in the gastrointestinal tract; therefore, the addition of fish protein hydrolysate (FPH) is suspected to influence the activity of microbiota, especially probiotics. Therefore, the presence of probiotics in the gastrointestinal tract affects the weight and quality of the broiler. This research aims to investigate the effect of administering fish protein hydrolysate as a dietary supplement on the composition of lactic acid bacteria (LAB), a potential probiotic candidate. This research was conducted in several stages, including the diversification of feeding broilers, the isolation and purification of LAB from the caecum and small intestine, primary characteristics, pathogenicity tests, and biochemical identification of LAB. Feed diversification was conducted by supplementing diets with 1%, 1.5%, and 2% FPH in 20-day-old for 7 days.. LAB from the small intestine and caecum samples were isolated on MRSA media by adding CaCO3. The Gram test, catalase test, and endospore staining test were carried out to characterize the suspected LAB primarily. The pathogenicity test was conducted by inoculating LAB on blood agar medium. Furthermore, biochemical tests are carried out using the KB020 kit. The results showed that the highest population of LAB in the small intestine (1.57 × 108 CFU/mL) was observed with 2% FPH supplementation. In comparison, the caecum yielded the highest population (1.22 × 108 CFU/mL) under 1.5% FPH. Giving 2% FPH  to broiler chicken feed provides a weight gain of 1.021 kg/head. The primary characteristics of the eight bacterial colony isolates suspected of being LAB were Gram-positive, catalase-negative, and did not form endospores. Eight LAB isolates of probiotic candidates were non-pathogenic as indicated by the occurrence of α-hemolysis and γ-hemolysis. Biochemical identification of probiotic candidates yielded four types of Lactobacillus, namely L. mucosae, L. frumenti, L. sanfranciscensis, and L. ferintoshensis. These LAB strains show promising probiotic potential for use as a feed additive in the broiler production system.
Co-Authors A. A. Istri Ratnawati AA. Istri Ratnadewi Achmad Sjaifullah Agung Budi Santoso Anak Agung Istri Ratnadewi Andika Ade Kurniawan, Andika Ade Andriana Kusuma Pertiwi Asnawati Asnawati Asnawati Aulia, Eldiani Bambang Piluharto Busroni Citra, Priscillia Denny Trias Utomo, Denny Trias Donatus Setyawan Purwo Handoko Dwi Indarti Esti Utarti Esti Utarti Eva Tyas Utami Febriani, Nurul Afifah Firdausa, Fita Kurnia Henry Adi Syahputra Sidabutar I Nyoman Adi Winata Ika Oktavianawati Kristiyono, Rimba Candra Kumalasari, Ardine Kumalasari, Ardine Letisya, Nanda Leyla Novita Brigiyanti Linda Faiqotul Himmah Malika Belkis Muhamad Kiki Afindia Joenata Muhammad Reza Nasrul Amaliyatun Naja NG. Krishnabudi Ni Nyoman Tri Puspaningsih Novita Andarini Nugraha, Ari Satya Nyoman Gede Krishnabudi R. Yuni Ristanti Rani Dian Medayani Riki Juni Krismiadi Rohmawati, Manis S. Sudarko Siti Nur Avida Sudarko Sudarko Sudarko Tri Agus Siswoyo Tri Mulyono Tri Mulyono Utari, Dwi Purwita Yeni Maulidah Muflihah Yudi Aris Sulisitiyo Zain, Romzi Al Amiri