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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK Biogenesis: Jurnal Ilmiah Biologi Global Medical and Health Communication LENSA (Lentera Sains): Jurnal Pendidikan IPA Indonesian Chimica Letters Life Science and Biotechnology

Esti Utarti

Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Jember

Author-ID : 2976204

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Dentistry Education Environmental Science Health Professions Immunology & microbiology Mathematics Nursing Physics Public Health

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SCREENING AND IDENTIFICATION ALKALY-CELLULOLYTIC MOLDS FROM RICE STRAW ON COASTAL-FIELD OF WATU ULO JEMBER Esti Utarti; Su’udah Hasanah; Siswanto Siswanto
Jurnal ILMU DASAR Vol 13 No 1 (2012)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

About 28 molds were obtained from rice straw on coastal-field of Watu Ulo Jember, were screened for their cellulolytic activities at pH alkaline. In semiquantitatively screening on CMC and Avicel plate at pH 8 showed that 27 isolates have cellulolytic activities. Based on clearing zone in CMC plate, isolates 7, 9, 14, 19 and 24 have higher cellulase (CMC-ase) activities index at pH alkaline. Further, in quantitatively examination using rice straw in Basic Salt Mandel’s modification medium showed isolate 19 (0,60 U/ml) that identified as Aspergillus terreus have higher FP-ase activity than isolate 7 (0,56 U/ml), 9 (0,55 U/ml), 14 (0,53 U/ml) and 24 (0,56 U/ml).

Characterization of Crude Protease Bacillus sp 31 Esti Utarti; Lina Nurita; Sattya Arimurti
Jurnal ILMU DASAR Vol 10 No 1 (2009)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.094 KB)

Bacillus sp 31 was bacteria which produce protease. Characterization of protease from Bacillus sp 31 i.e. pH, temperature, influence of metal ion, enzyme kinetic and enzyme termostability is important to get optimal enzyme activity. Protease activity showed values 146.40 U/ml on pH 9 and optimal temperature 60°C by value. Protease activity increased by addition of 159.50 U/ml Fe2+, but its activity decreased by addition of Mg2+, Cu2+, Ca2+, Al2+, Zn2+ dan Mn2+. Maximal velocity (Vmax) of enzyme-catalysed reactions was 21.32 U/ml with Km 1.5x10-3 mg/ml (Michaels-Menten Kinetic). Protease was very stable at 60°C for 4 hours of incubation and 7 hours of half-time.

Growth Pattern and Degradation Activity of Caffeine-degrading Bacteria Consortium Suksma, Nadhea Ayu; Utarti, Esti; Arimurti, Sattya
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.32609

Caffeine-degrading bacteria can be used as agents to degrade caffeine, thereby reducing the concentration of caffeine in organic waste. The decomposition process is carried out by a single bacterium or a consortium of bacteria. Caffeine-degrading bacteria from Sempol, Bondowoso, namely Acinetobacter gerneri KAFS 47, Paracoccus denitrificans KAFS 16 and Pseudomonas plecoglossicida KAFS 34, could be used as a bacterial consortium to promote caffeine degradation. The aim of this study was to analyze associations between caffeine-degrading bacteria isolates, bacterial resistance to antibiotics, growth patterns, and caffeine degradation of a consortium of caffeine-degrading bacteria, and the correlation of bacterial growth with caffeine degradation. The research method used is an analysis of the association between isolates, the development of bacterial consortium growth patterns, and their analysis based on antibiotic resistance, patterning of caffeine degradation, and correlation test (Pearson) of bacterial growth with caffeine degradation. The result of the association test between bacteria showed that the three bacteria had the potential to be used as a consortium of caffeine-degrading bacteria. A. Gerneri, P. denitrificans, and P. plecoglossicida were resistant to the antibiotic cefixime (100 ppm), erythromycin (50 ppm), lincomycin (50 ppm), metronidazole (50 ppm), and sanprima (50 ppm). The growth of the bacterial consortium (54.779 CFU/mL) was higher than that of P. plecoglossicida (49.277 CFU/mL) and lower than that of A. gerneri (93.481 CFU/mL) and P. denitrificans (84.940 CFU/mL) in incubation time of 4 days. However, the consortium of bacteria and P. plecoglossicida were able to degrade caffeine 24 hours faster (3 days) than the other two single isolates (4 days) to degrade 2.5 g/L caffeine in media to 0%. Bacterial growth due to caffeine degradation has a perfect correlation value (>0. 950) and is negative.

Using Lignosellulose Waste as a Xylanase Production Media of Mold Isolated from Rice Straw of Coastal-field Utarti, Esti; Siswanto, S.
Jurnal ILMU DASAR Vol 19 No 2 (2018)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Hemicellulose is one of lignocellulose waste component, so that xylanase is one of importance enzyme of lignocellulose waste biodegradation. Molds as main decomposer lignosellulose waste has enzyme activities higher than yeast and bacteria. The aim of the research is to find mold that have xylanolitic activity using lignocellulose waste as media production. The research consist of isolations and screening mols from coastal-field of watu Ulo Jember, xylanase production using lignocellulose waste and idntification of mold which has the highes xylanase activity. A total of 66 molds isolated from rice straw in coastal-field of Watu Ulo Jember. There were screened for their xylanase activity. In semiquantitatively screen on Oat Spelt Xylan plate, the result showed that 62 have xilanolytic activities. Based on clearing zone production, isolates ESW A1 (3.2), ESW A5 (3.1), ESW C 16 (3.26), ESW D4 (3.0) and ESW D15 (3.21) have xilanase activity index higher than others. Furthermore, quantitative analysis using wheat bran, rice straw and baggase in basic salt Mandel’s modification media showed that xylanase activity of isolate ESW D4 was higher on rice straw 3% as substrate production with activity 2.66 U/mL. Isolate ESW D4 identified as Aspergillus foetidus so that called as Aspergillus foetidus ESW D4. Keywords: rice straw, coastal-field, Aspergillus foetidus ESW-D

Partial Analysis of Probiotic Character of Lactic Acid Bacteria from the Intestines of Broilers Supplemented with Fish Protein Hydrolysate (FPH) Nashrullah, Shafa; Utami, Eva Tyas; Purniasari, Fina Yunita; Anggitasari, Dhanti Fatma; Utarti, Esti
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.53701

This study aims to analyze partial characteristics of probiotics lactic acid bacteria (LAB) from broiler intestines supplemented with fish protein hydrolysate (FPH) 2% (v/w) in feed. A total of 17 LAB isolates were successfully isolated and characterized morphologically and biochemically (catalase test). The isolate showed diverse morphological and biochemical properties, obtained as many as seven isolates that met the partial characteristics of probiotics, namely U10, U11, U13, U16, U31, U33, and U37 which were Gram-positive and no endospores and catalase formations were found. The tolerance test for acid pH and NaCl performed on the seven BAL isolates showed a diverse survival rate with U11 showing the highest tolerance at pH 2 (11.4%) and NaCl concentration up to 8% (32.4%). These findings highlight seven LAB isolates with partial probiotic properties, providing new insights into the development of probiotic candidates from broiler guts to improve gastrointestinal health and productivity.

Production and Characterization od Xylanase from Actinomyces ATG 70 Using CornCob Xylan Substrate Farrennina, Tasya Preira; Putri, Amelia Fahreza; Arimurti, Sattya; Winarsa, Rudju; Utarti, Esti
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.53702

Xilan is a polysaccharide that can be used as a substrate for producing xylanase. Xylan can be obtained from processing xylan-rich agricultural waste such as corn cobs, which contain 12.4% xylan. Xilan can be hydrolyzed into xylose and xylooligosaccharide (XOS) using the enzyme Xilanase. Actinomycetes have dominant xylanolytic species, Actinomyces ATG 70 has semi-qualitative xylanolytic ability with an enzyme activity index of 3.21±0.55 in xylan media. This study was conducted by extracting xylan from corn cobs, rejuvenating Actinomyces ATG 70, preparing the inoculum, creating a standard curve, producing xylanase with pH optimization of the medium, testing xylanase activity, characterizing the pH and temperature of crude xylanase, and identifying the morphology and biochemical characteristics of Actinomyces ATG 70. Optimization of the pH of the xylanase production medium was achieved at pH 8, with xylanase activity of 10.07±0.13 U/mL. The crude xylanase was characterized for pH and temperature effects, and the optimal pH for crude xylanase was found to be pH 6 with xylanase activity of 7.64±0.66 U/mL, and the optimal temperature was 50°C with xylanase activity of 11.17±0.33 U/mL. The identification results showed that Actinomyces ATG 70 belongs to Gram-positive bacteria, with a positive catalase test, white colonies, cream-colored aerial mycelium, and cream-colored pigmentation. The spore structure consists of long chains of conidia, streptococcus-shaped cells, and can form aerial mycelium, thus the isolate belongs to the genus Streptomyces.

Co-Authors AA. Istri Ratnadewi Anggitasari, Dhanti Fatma Anja Meryandini Antonius Suwanto Ayuningtyas, Tantri Raras Azhari, Fitri Bambang Sugiharto Dina Amalia Syahidah Dwi Setyati Edia F.D Edia, Edia F.D Eva Tyas Utami Evi Umayah Ulfa Farrennina, Tasya Preira Ferymon Mahulette Ida Ayu Putu Sri Widnyani Izzay Afkarina Jefri Nur Hidayat Kahar Muzakhar Kartika Senjarini Lina Nurita Maggy T Suhartono Media Ningtyas, Rinda Nashrullah, Shafa Purniasari, Fina Yunita Putri, Amelia Fahreza Riki Juni Krismiadi S Siswanto S Sutoyo S. Siswanto Saniyah Fatkhul Alim Sattya Arimurti Siswanto Siswanto Suksma, Nadhea Ayu Sutoyo Sutoyo Sutoyo Sutoyo Su’udah Hasanah Tri Santi Kurnia Winarsa, Rudju Wuryanti Handayani

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